Fluorescence microscopy as a tool for in situ life detection

The identification of extant and, in some cases, extinct bacterial life is most convincingly and efficiently performed with modern high-resolution microscopy. Epifluorescence microscopy of microbial autofluorescence or in conjunction with fluorescent dyes is among the most useful of these techniques. We explored fluorescent labeling and imaging of bacteria in rock and soil in the context of in situ life detection for planetary exploration. The goals were two-fold: to target non-Earth-centric biosignatures with the greatest possible sensitivity and to develop labeling procedures amenable to robotic implementation with technologies that are currently space qualified. A wide panel of commercially available dyes that target specific biosignature molecules was screened, and those with desirable properties (i.e., minimal binding to minerals, strong autofluorescence contrast, no need for wash steps) were identified. We also explored the potential of semiconductor quantum dots (QDs) as bacterial and space probes. A specific instrument for space implementation is suggested and discussed.     

The Dynamic Albedo of Neutrons (DAN) experiment for NASA's 2009 Mars Science Laboratory

We present a summary of the physical principles and design of the Dynamic Albedo of Neutrons (DAN) instrument onboard NASA's 2009 Mars Science Laboratory (MSL) mission. The DAN instrument will use the method of neutron-neutron activation analysis in a space application to study the abundance and depth distribution of water in the martian subsurface along the path of the MSL rover.     

Mineral and nitrogen balance study: results of metabolic observations on Skylab II 28-day orbital mission

Prediction that the various stresses of flight, particularly weightlessness, would bring about significant derangements in the metabolism of the musculoskeletal system has been based on various observations of long-term immobilized or inactive bed rest. The only attempt at controlled measurement of metabolic changes in space prior to Skylab, a study during the 14-day Gemini VII flight, revealed rather modest losses of important elements. The three astronauts of Skylab II consumed a planned day-by-day, quite constant, dietary intake of major metabolic elements in mixed foods and beverages and provided virtually complete collections of excreta for 31 days preflight, during the 28 days inflight, and for 17 days postflight. Analyses showed that, in varying degree among the crewmen, urinary calcium increased gradually during flight in a pattern similar to that observed in bed-rest studies: the mean plateau peak of urinary calcium excretion in the latter part of flight was double preflight levels. Fecal calcium excretion did not change significantly, but calcium balance, owing to the urinary calcium rise, became either negative or less positive than in preflight measurement. Increased excretion and negative balance of nitrogen and phosphorus indicated appreciable loss of muscle tissue in all three crewmen. Significant losses also occurred inflight in potassium, sodium, and magnesium. Based on the similarity in pattern and degree between these observations and those in bed rest of the losses in calcium, phosphorus, and nitrogen, musculoskeletal integrity would not be threatened in space flights of up to at least 3 months. However, if similar changes occur, indicative of continuing losses of these elements, in the planned Skylab flights for considerably more than 28 days, concern for capable musculoskeletal function should be serious for flights of very many months' duration, and greater research attention will need to be given to development of protective counter-measures.     

The ‘soot line’: Destruction of presolar polycyclic aromatic hydrocarbons in the terrestrial planet-forming region of disks

Interstellar material is highly processed when subjected to the physical conditions that prevail in the inner regions of protoplanetary disks, the potential birthplace of habitable planets. Polycyclic aromatic hydrocarbons (PAHs) are abundant in the interstellar medium, and they have also been observed in the disks around young stars, with evidence for some modification in the latter. Using a chemical model developed for sooting flames, we have investigated the chemical evolution of PAHs in warm (1000–2000 K) and oxygen-rich (C/O < 1) conditions appropriate for the region where habitable planets may eventually form. Our study focuses on (1) delineating the conditions under which PAHs will react and (2) identifying the key reaction pathways and reaction products characterizing this chemical evolution. We find that reactions with H, OH and O are the main pathways for destroying PAHs over disk timescale at temperatures greater than about 1000 K. In the process, high abundances of C₂H₂ persist over long timescales due to the kinetic inhibition of reactions that eventually drive the carbon into CO, CO₂ and CH₄. The thermal destruction of PAHs may thus be the cause of the abundant C₂H₂ that has been observed in disks. We propose that protoplanetary disks have a ‘soot line’, within which PAHs are irreversibly destroyed via thermally-driven reactions. The soot line will play an important role, analogous to that of the ‘snow line’, in the bulk carbon content of meteorites and habitable planets.     

Galileo Probe Nephelometer experiment

The objective of the Nephelometer Experient aboard the Probe of the Galileo mission is to explore the vertical structure and microphysical properties of the clouds and hazes in the atmosphere of Jupiter along the descent trajectory of the Probe (nominally from 0.1 to > 10 bars). The measurements, to be obtained at least every kilometer of the Probe descent, will provide the bases for inferences of mean particle sizes, particle number densities (and hence, opacities, mass densities, and columnar mass loading) and, for non-highly absorbing particles, for distinguishing between solid and liquid particles. These quantities, especially the location of the cloud bases, together with other quantities derived from this and other experiments aboard the Probe, will not only yield strong evidence for the composition of the particles, but, using thermochemical models, for species abundances as well. The measurements in the upper troposphere will provide ground truth data for correlation with remote sensing instruments aboard the Galileo Orbiter vehicle. The instrument is carefully designed and calibrated to measure the light scattering properties of the particulate clouds and hazes at scattering angles of 5.8°, 16°, 40°, 70°, and 178°. The measurement sensitivity and accuracy is such that useful estimates of mean particle radii in the range from about 0.2 to 20 can be inferred. The instrument will detect the presence of typical cloud particles with radii of about 1.0 , or larger, at concentrations of less than 1 cm³.Deceased.     

Potential of IRNSS/NavIC L5 signals for ionospheric studies

Indian Space Research Organization (ISRO) has developed an indigenous system named Indian Regional Navigation Satellite System (IRNSS) or NavIC (Navigation with Indian Constellation), that consists of 7 satellites and transmits navigation signal in L and S bands. ISRO, for validation of the system, has installed many IGS (IRNSS/GPS/SBAS) receivers scattered over the Indian region. Using preliminary data from two geographically widely separated stations over India, this paper presents the results on studies on parameters of IRNSS signal quality and discusses how these parameters may be used to study the ionospheric behavior over the Indian region. The results show the importance and advantages of using IRNSS data for such studies.     

Monopulse Estimation of the Centroid of an Ensemble of Radar Scatterers

The use of data obtained by a monopulse radar to estimate the location of the radar cross-section centroid of an ensemble of scatterers is discussed. Both dish and phased-array antenna radars are treated. Expressions for the bias and variance of the centroid estimates are presented, including the effects of the radar receiver and beam pattern characteristics, receiver noise, and the video waveform sampling granularity, as well as the target properties. The monopulse tracking approach discussed here is contrasted with a raster scan approach presented previously.     

Position and Velocity Estimation Via Bearing Observations

The problem of performing target motion analysis using noisy bearing measurements derived from multiple observation platforms or from a single moving observer is addressed. For the latter case, the properties of an estimator based on a Cartesian model of the process are detailed. Methods of providing estimates both before an observer maneuver, when the process is unobservable, and following an observer maneuver are developed. The results of an experimental study are presented.