Stability of amino acids and their oligomerization under high-pressure conditions: implications for prebiotic chemistry

The polymerization of amino acids leading to the formation of peptides and proteins is a significant problem for the origin of life. This problem stems from the instability of amino acids and the difficulty of their oligomerization in aqueous environments, such as seafloor hydrothermal systems. We investigated the stability of amino acids and their oligomerization reactions under high-temperature (180-400°C) and high-pressure (1.0-5.5?GPa) conditions, based on the hypothesis that the polymerization of amino acids occurred in marine sediments during diagenesis and metamorphism, at convergent margins on early Earth. Our results show that the amino acids glycine and alanine are stabilized by high pressure. Oligomers up to pentamers were formed, which has never been reported for alanine in the absence of a catalyst. The yields of peptides at a given temperature and reaction time were higher under higher-pressure conditions. Elemental, infrared, and isotopic analyses of the reaction products indicated that deamination is a key degradation process for amino acids and peptides under high-pressure conditions. A possible NH(3)-rich environment in marine sediments on early Earth may have further stabilized amino acids and peptides by inhibiting their deamination.     

A challenge to the highest balloon altitude

Development of a balloon to fly at higher altitudes is one of the most attractive challenges for scientific balloon technologies. After reaching the highest balloon altitude of 53.0 km using the 3.4 μm film in 2002, a thinner balloon film with a thickness of 2.8 μm was developed. A 5000 m³ balloon made with this film was launched successfully in 2004. However, three 60,000 m³ balloons with the same film launched in 2005, 2006, and 2007, failed during ascent. The mechanical properties of the 2.8 μm film were investigated intensively to look for degradation of the ultimate strength and its elongation as compared to the other thicker balloon films. The requirement of the balloon film was also studied using an empirical and a physical model assuming an axis-symmetrical balloon shape and the static pressure. It was found that the film was strong enough. A stress due to the dynamic pressure by the wind shear is considered as the possible reason for the unsuccessful flights. A 80,000 m³ balloon with cap films covering 9 m from the balloon top will be launch in 2011 to test the appropriateness of this reinforcement.     

A new balloon base in Japan

Since 1971, numerous balloons have been launched from the Japanese balloon base, the Sanriku Balloon Center (SBC). Through these years, balloon technologies have been developed continuously and many scientific achievements have resulted. Recently, however, because of the limited area of the launching pad of the SBC, we have been faced with the difficulty of safely launching large balloons. To solve this issue, we decided to move the balloon base from the SBC to the Taiki Aerospace Research Field (TARF) in northern Japan. The TARF had an existing huge hanger and a paved launch pad capable of being utilised for balloon operations. To evolve the TARF into a new balloon base, new balloon facilities have been constructed at the TARF and equipment was transferred from the SBC to the TARF during July 2007 and March 2008. The SBC was closed in September 2007, and the new base became operational in May 2008. The new base at the TARF is designed to launch larger balloons with greater safety and to perform balloon operations more effectively than ever before. In the summer of 2008, we carried out the first series of the balloon campaign at the TARF, and succeeded in two engineering flights of stratospheric balloons. By the success of these flights, we have verified that the whole system of the new balloon base is well established.     

Properties of tandem balloons connected by extendable suspension wires

The tandem balloon system has been known as a candidate system for long duration flight balloons. In this paper, the properties of the system are analytically studied in a new way by introducing an extendable suspension wire in the Sky Anchor configuration, which consists of a zero-pressure main balloon suspending a payload and a super-pressure balloon suspended below the payload. It was found that extension of the suspension wire between the payload and the super-pressure balloon can extend the capability of the tandem system; the altitude of the zero-pressure balloon can be changed without any consumables except some energy, and the day–night oscillation of the balloon altitude can be suppressed. This property is useful as the vehicle for long duration flights. It is also pointed out that the method to control the altitude of a balloon using an additional suspended super-pressure balloon can also be applied for super-pressure balloons.     

Microbial survival: the paleome: a sedimentary genetic record of past microbial communities

Molecular genetic methods were used to analyze the remnants of microbial ecosystems contained within an ancient oceanic microbial habitat that was recovered from a continental drilled core of black shale approximately 100 million years in age. Bacterial ribosomal RNA genes were vertically amplified from the six different depths of a black shale core associated with a phosphate-rich stratum, defined as one of the mid-Cretaceous oceanic anoxic events (OAEs). Although the black shale core was recovered from a terrestrial coring effort, the recovered 16S rRNA gene sequences showed affinity to microbial communities previously seen in deep-sea sedimentary environments (i.e., the microbial assemblage was easily recognizable as a marine community). In particular, a number of 16S rRNA gene clones of oceanic sulfate-reducing bacteria within the delta-Proteobacteria predominated at the OAE layer. The recovered bacterial DNA signatures are consistent with the interpretation that the sequences are derived from the past microbial communities buried in either sea-bottom or subseafloor environments during the sedimentation process and, after ceasing growth, preserved until the present.     

Search for primordial antiparticles with BESS

The Balloon-borne Experiment with a Superconducting Spectrometer (BESS) has been carried out to search for primordial antiparticles in cosmic rays. In ten flights from 1993 to 2004, it measured the cosmic-ray antiproton spectrum in the energy range 0.1–4.2 GeV at various solar activity conditions. It also searched for antideuterons and antihelium nuclei, and it made precise measurement of cosmic-ray particle spectra. The BESS program has been extended to long duration balloon (LDB) flights in Antarctica (BESS-Polar) with the goal of achieving unprecedented sensitivity in the search for primordial antiparticles. This report describes recent results from BESS and progress of the BESS-Polar program.     

Search for antihelium: Progress with BESS

In this paper, we report searches for antihelium in cosmic rays using two recently flown magnetic rigidity spectrometers. BESS-TeV had extended rigidity with an MDR of 1.4 TV and had a flight duration of one day. BESS-Polar was optimized for collecting power. It was flown for 8.5 days and had an MDR of 240 GV. The former flight allows us to explore a previously unexplored rigidity band and the latter flight yields a factor of three improvement in the overall BESS limit. No antihelium candidate was found in the rigidity ranges of 1–500 GV, and 0.6–20 GV, among 7 × 10⁴ events taken with BESS-TeV, and 8 × 10⁶ events taken with BESS-Polar, respectively.     

Cosmic ray H/H ratio measured from BESS in 2000 during solar maximum

The Balloon-borne Experiment with a Superconducting Spectrometer (BESS) was flown from Lynn Lake, Manitoba, Canada in August, 2000, during the maximum solar modulation period, with an average residual atmospheric overburden of 4.3 g/cm². Precise spectral measurements of cosmic ray hydrogen isotopes from 0.178 GeV/n to 1.334 GeV/n were made during the 28.7 h of flight. This paper presents the measured energy spectra and their ratio, ²H/¹H. The results are also compared with previous measurements and theoretical predictions.     

The pGAPS experiment: An engineering balloon flight of prototype GAPS

The General Anti-Particle Spectrometer (GAPS) project is being carried out to search for primary cosmic-ray antiparticles especially for antideuterons produced by cold dark matter. GAPS plans to realize the science observation by Antarctic long duration balloon flights in the late 2010s. In preparation for the Antarctic science flights, an engineering balloon flight using a prototype of the GAPS instrument, “pGAPS”, was successfully carried out in June 2012 in Japan to verify the basic performance of each GAPS subsystem. The outline of the pGAPS flight campaign is briefly reported.