Presynaptic transporter-mediated release of glutamate evoked by the protonophore FCCP increases under altered gravity conditions

High-affinity Na⁺-dependent glutamate transporters of the plasma membrane mediate the glutamate uptake into neurons, and thus maintain low levels of extracellular glutamate in the synaptic cleft. The study focused on the release of glutamate by reversal of Na⁺-dependent glutamate transporters from rat brain nerve terminals (synaptosomes) under conditions of centrifuge-induced hypergravity. Flow cytometric analysis revealed similarity in the size and cytoplasmic granularity between synaptosomal preparations obtained from control and G-loaded animals (10 G, 1 h). The release of cytosolic l-[¹⁴C]glutamate from synaptosomes was evaluated using the protonophore FCCP, which dissipated synaptic vesicle proton gradient, thus synaptic vesicles were not able to keep glutamate inside and the latter enriched cytosol. FCCP per se induced the greater release of l-[¹⁴C]glutamate in hypergravity as compared to control (4.8 ± 1.0% and 8.0 ± 1.0% of total label). Exocytotic release of l-[¹⁴C]glutamate evoked by depolarization was reduced down to zero after FCCP application under both conditions studied. Depolarization stimulated release of cytosolic l-[¹⁴C]glutamate from synaptosomes preliminary treated with FCCP was considerably increased from 27.0 ± 2.2% of total label in control to 35.0 ± 2.3% in hypergravity. Non-transportable inhibitor of glutamate transporter dl-threo-β-benzyloxyaspartate was found to significantly inhibit high-KCl and FCCP-stimulated release of l-[¹⁴C]glutamate, confirming the release by reversal of glutamate transporters. The enhancement of transporter-mediated release of glutamate in hypergravity was found to result at least partially from the inhibition of the activity of Na/K-ATPase in the plasma membrane of synaptosomes. We suggested that hypergravity-induced alteration in transporter-mediated release of glutamate indicated hypoxic injury of neurons.     

Relation between galactic and solar cosmic radiation at aviation altitude

Cosmic radiation bombards us at high altitude with ionizing particles; the radiation has a galactic component, which is normally dominant, and a component of solar origin. Cosmic ray particles are the primary source of ionization in the atmosphere above 1 km; below 1 km radon is a dominant source of ionization in many areas.     

Optical,ultraviolet, and infrared observations of isolated neutron stars

Forty years passed since the optical identification of the first isolated neutron star (INS), the Crab pulsar. 25 INSs have been now identified in the optical (O), near-ultraviolet (nUV), or near-infrared (nIR), hereafter UVOIR, including rotation-powered pulsars (RPPs), magnetars, and X-ray-dim INSs (XDINSs), while deep investigations have been carried out for compact central objects (CCOs), Rotating RAdio transients (RRATs), and high-magnetic field radio pulsars (HBRPs). In this review I describe the status of UVOIR observations of INSs, their emission properties, and I present the results from recent observations.     

Contamination of short GRBs by giant magnetar flares: Significance of downward revision in distance to SGR 1806–20

We highlight how the downward revision in the distance to the star cluster associated with SGR 1806–20 by Bibby et al. (2008) reconciles the apparent low contamination of BATSE short GRBs by intense flares from extragalactic magnetars without recourse to modifying the frequency of one such flare per 30 years per Milky Way galaxy. We also discuss the variety in progenitor initial masses of magnetars based upon cluster ages, ranging from ∼50 M_⊙ for SGR 1806–20 and AXP CXOU J164710.2–455216 in Westerlund 1 to ∼17 M_⊙ for SGR 1900+14 according to Davies et al. (2009) and presumably also 1E 1841–045 if it originated from one of the massive RSG clusters #2 or #3.     

Two magnetars: SGR 1627–41 and 1E 1547–5408

We describe the results obtained with Target of Opportunity observations of the galactic sources SGR 1627–41 and 1E 1547–5408. These two transients show several similarities supporting the interpretation of Anomalous X-ray Pulsars and Soft Gamma-ray Repeaters as a single class of strongly magnetized neutron stars.     

Using gamma-ray burst prompt emission to probe relativistic shock acceleration

It is widely accepted that the prompt transient signal in the 10 keV–10 GeV band from gamma-ray bursts (GRBs) arises from multiple shocks internal to the ultra-relativistic expansion. The detailed understanding of the dissipation and accompanying acceleration at these shocks is a currently topical subject. This paper explores the relationship between GRB prompt emission spectra and the electron (or ion) acceleration properties at the relativistic shocks that pertain to GRB models. The focus is on the array of possible high-energy power-law indices in accelerated populations, highlighting how spectra above 1 MeV can probe the field obliquity in GRB internal shocks, and the character of hydromagnetic turbulence in their environs. It is emphasized that diffusive shock acceleration theory generates no canonical spectrum at relativistic MHD discontinuities. This diversity is commensurate with the significant range of spectral indices discerned in prompt burst emission. Such system diagnostics are now being enhanced by the broad-band spectral coverage of bursts by the Fermi Gamma-Ray Space Telescope; while the Gamma-Ray Burst Monitor (GBM) provides key diagnostics on the lower energy portions of the particle population, the focus here is on constraints in the non-thermal, power-law regime of the particle distribution that are provided by the Large Area Telescope (LAT).     

In situ analysis of Europa ices by short-range melting probes

A key aspect for understanding the astrobiological potential of planets and moons in the Solar system is the analysis of material embedded in or underneath icy layers on the surface. In particular in case of the icy crust of Jupiters moon Europa such investigation would be of greatest interest. For a Europa lander to be launched in the 2020–2030 timeframe, we propose to use a simplified instrumented melting probe which is able to access and sample depths of a few meters without the necessity of heavy and complicated drilling equipment.     

Study of GNSS-measured ionospheric total electron content variations generated by powerful HF-heating

The purpose of this work is to report the experimental evidences for the influence of perturbations in the electron density in the dayside mid-latitude ionosphere, that are caused by high-frequency heating of the F2 layer, on the GNSS signals. The experiments were carried out at the Sura heater (Radio Physical Research Institute, N. Novgorod). During the sessions of ionospheric heating with different time modulations of the radiated power the rays linking the navigational satellites with the ground receiver intersected the heated region. Variations in the total electron content (TEC) were studied; these variations are proportional to the reduced phases of navigational signals. It is shown that with the square-wave modulation of the radiated power (with periods of 1, 6, 10 and 15 min), perturbations with periods of the main modulation of heating and its harmonics appear in the spectrum of TEC variations. Examples are presented of identification of the heating-induced variations in TEC, including determination of the amplitudes and time characteristics of these variations.     

Return to Europa: Overview of the Jupiter Europa orbiter mission

Missions to explore Europa have been imagined ever since the Voyager mission first suggested that Europa was geologically very young. Subsequently, the Galileo spacecraft supplied fascinating new insights into this satellite of Jupiter. Now, an international team is proposing a return to the Jupiter system and Europa with the Europa Jupiter System Mission (EJSM). Currently, NASA and ESA are designing two orbiters that would explore the Jovian system and then each would settle into orbit around one of Jupiter’s icy satellites, Europa and Ganymede. In addition, the Japanese Aerospace eXploration Agency (JAXA) is considering a Jupiter magnetospheric orbiter and the Russian Space Agency is investigating a Europa lander.     

Compact frequency standard using atoms trapped on a chip

We present a compact atomic frequency standard based on the interrogation of magnetically trapped ⁸⁷Rb atoms. Two photons, in the microwave and radiofrequency domain excite the atomic transition. At a magnetic field of 3.23 G this transition from ∣F = 1, m_F = −1〉 to ∣F = 2, m_F = 1〉 is 1st order insensitive to magnetic field variations. Long Ramsey interrogation times can thus be achieved, leading to a projected stability in the low 10⁻¹³ at 1 s. This makes this device a viable alternative to LITE and HORACE as a good candidate for replacing or complementing the rubidium frequency standards and passive hydrogen masers already on board of the GPS, GLONASS, and GALILEO satellites. Here we present preliminary results. We use an atom chip to cool and trap the atoms. A coplanar waveguide is integrated to the chip to carry the Ramsey interrogation signal, making the physics package potentially as small as (5 cm)³. We describe the experimental apparatus and show preliminary Ramsey fringes of 1.25 Hz linewidth. We also show a preliminary frequency stability σ_y = 1.5 × 10⁻¹²τ^−1/2 for 10 < τ < 10³ s. This represents one order of magnitude improvement with respect to previous experiments.