Repair of DNA double-strand breaks and cell killing by charged particles.

It has been suggested that it is not simple double-strand breaks (dsb) but the non-reparable breaks which correlate well with the high biological effectiveness of high LET radiations for cell killing (Kelland et al., 1988; Radford, 1986). We have compared the effects of charged particles on cell death in 3 pairs of cell lines which are normal or defective in the repair of DNA dsbs. For the cell lines SL3-147, M10, and SX10 which are deficient in DNA dsb repair, RBE values were close to unity for cell killing induced by charged particles with linear energy transfer (LET) up to 200 keV/micrometer and were even smaller than unity for the LET region greater than 300 keV/micrometer. The inactivation cross section (ICS) increased with LET for all 3 pairs. The ICS of dsb repair deficient mutants was always larger than that of their parents for all the LET ranges, but with increasing LET the difference in ICS between the mutant and its parent became smaller. Since a small difference in ICS remained at LET of about 300 keV/micrometer, dsb repair may still take place at this high LET, even if its role is apparently small. These results suggest that the DNA repair system does not play a major role in protection against the attack of high LET radiations and that a main muse of cell death is non-reparable dsb which are produced at a higher yield compared with low LET radiations. No correlation was observed between DNA content or nuclear area and ICS.     

Low fluence.

The question of the appropriate extrapolation to low dose has long been a subject of controversy. A linear no-threshold model is favored by regulatory bodies as the basis of RBE assignments and estimates of radiation hazards to the general population. This model is largely supported by extensive application of the linear-quadratic survival formula "fitted" statistically to a wide variety of experimental data obtained at doses typically exceeding 1 Gy, and then extrapolated to mGy for practical applications, and even to the prediction of hazards from single electrons. Such extrapolations are questionable at best, and may even prove hazardous for risk evaluations. Fluence and geometry rather than dose based data are proposed as a basis for a limiting "threshold" for a "low dose" extrapolation. The proposed threshold is one where the fluence of particles is one per square micron, where on average only 2/3 of the 1 micrometers2 pixels covering an irradiated area are traversed by one or more particles. The corresponding dose threshold is determined by the LET of the bombarding radiation. For relativistic electrons this dose is about 0.032 Gy.     

ExoMars 2016 Schiaparelli Module Trajectory and Atmospheric Profiles Reconstruction

On 19^th October 2016 Schiaparelli module of the ExoMars 2016 mission flew through the Mars atmosphere. After successful entry and descent under parachute, the module failed the last part of the descent and crashed on the Mars surface. Nevertheless the data transmitted in real-time by Schiaparelli during the entry and descent, together with the entry state vector as initial condition, have been used to reconstruct both the trajectory and the profiles of atmospheric density, pressure and temperature along the traversed path.The available data-set is only a small sub-set of the whole data acquired by Schiaparelli, with a limited data rate (8 kbps) and a large gap during the entry because of the plasma blackout on the communications.This paper presents the work done by the AMELIA (Atmospheric Mars Entry and Landing Investigations and Analysis) team in the exploitation of the available inertial and radar data. First a reference trajectory is derived by direct integration of the inertial measurements and a strategy to overcome the entry data gap is proposed. First-order covariance analysis is used to estimate the uncertainties on all the derived parameters. Then a refined trajectory is computed incorporating the measurements provided by the on-board radar altimeter.The derived trajectory is consistent with the events reported in the telemetry and also with the impact point identified on the high-resolution images of the landing site.Finally, atmospheric profiles are computed tacking into account the aerodynamic properties of the module. Derived profiles result in good agreement with both atmospheric models and available remote sensing observations.     

The Relativistic Proton Spectrometer (RPS) for the Radiation Belt Storm Probes Mission

The Relativistic Proton Spectrometer (RPS) on the Radiation Belt Storm Probes spacecraft is a particle spectrometer designed to measure the flux, angular distribution, and energy spectrum of protons from ～60 MeV to ～2000 MeV. RPS will investigate decades-old questions about the inner Van Allen belt proton environment: a nearby region of space that is relatively unexplored because of the hazards of spacecraft operation there and the difficulties in obtaining accurate proton measurements in an intense penetrating background. RPS is designed to provide the accuracy needed to answer questions about the sources and losses of the inner belt protons and to obtain the measurements required for the next-generation models of trapped protons in the magnetosphere. In addition to detailed information for individual protons, RPS features count rates at a 1-second timescale, internal radiation dosimetry, and information about electrostatic discharge events on the RBSP spacecraft that together will provide new information about space environmental hazards in the Earth’s magnetosphere.     

The organic aerosols of Titan

A dark reddish organic solid, called tholin, is synthesized from simulated Titanian atmospheres by irradiation with high energy electrons in a plasma discharge. The visible reflection spectrum of this tholin is found to be similar to that of high altitude aerosols responsible for the albedo and reddish color of Titan. The real (n) and imaginary (k) parts of the complex refractive index of thin films of Titan tholin prepared by continuous D.C. discharge through a 0.9 N₂/0.1 CH₄ gas mixture at 0.2 mb is determined from x-ray to microwave frequencies. Values of n (1.65) and k (0.004 to 0.08) in the visible are consistent with deductions made by ground-based and spaceborne observations of Titan. Many infrared absorption features are present in k(λ), including the 4.6 μm nitrile band. Molecular analysis of the volatile component of this tholin was performed by sequential and non-sequential pyrolytic gas chromatography/mass spectrometry. More than one hundred organic compounds are released; tentative identifications include saturated and unsaturated aliphatic hydrocarbons, substituted polycyclic aromatics, nitriles, amines, pyrroles, pyrazines, pyridines, pyrimidines, and the purine, adenine. In addition, acid hydrolysis produces a racemic mixture of biological and non-biological amino acids. Many of these molecules are implicated in the origin of life on Earth, suggesting Titan as a contemporary laboratory environment for prebiological organic chemistry on a planetary scale.     

Fuel consumption and collision avoidance strategy in multi-static orbit formations

This paper analyses the fuel consumption of interferometric radar missions employing small satellite formations like, e.g., Cross-track Pendulum, Cartwheel, CarPe, or Trinodal Pendulum. Individual analytic expressions are provided for each of the following contributions: separation from a simultaneously injected master satellite, formation set-up, orbit maintenance, formation maintenance, and distance maintenance. For this, a general system of equations is derived describing the relative motion of the small satellites in a co-rotating reference frame. The transformation into Keplerian elements is carried out. To evaluate fuel consumption, three master satellites are assumed in different orbital heights, which are typical for Earth observation missions. The size of the exemplarily analysed formations is defined by remote sensing aspects and their respective fuel requirements are estimated. Furthermore, a collision avoidance concept is introduced, which includes a formation separation and formation set-up after a desired time period.     

Cardiovascular effects of simulated zero-gravity in humans

Head-down and head-up [correction of heat-up] tilted bedrest (5 degrees) and head out water immersion (HOWI) for 6 hr were compared. Parameters: Cardiac output (rebreathing method), blood pressure (arm cuff), forearm blood flow (venous occlusion plethysmography), total peripheral (TPR), and forearm vascular (FVR) resistances, Hct, Hb, relative plasma volume (PV) changes, and plasma catecholamines (single-isotope assay). During HOWI there was as expected a decrement in TPR, FVR, Mean arterial pressure (MAP, from 100 to 80 mmHg), Hct, and PV, and--as a new finding--catecholamines, which were 30-50% lower compared with both +5 and -5 degrees bedrest. During head down tilt, MAP was elevated (to 100-110 mmHg) and catecholamines did not fall, while TPR and EVR slowly decreased over 6 hr. HOWI is a stronger stimulus than -5 degrees bedrest, probably because HOWI elevates central venous pressure more markedly emptying the peripheral veins, while bedrest permits a distension of veins, which induces an increase in sympathetic nervous activity.