Evaluation of bleomycin-induced chromosome aberrations under simulated microgravity conditions in human lymphocytes using "FISH" techniques

In the present investigation we report the effects of simulated microgravity conditions (clinostat) on the induction of chromosomal aberrations in human lymphocytes in vitro by (R) Bleomycin. Chromosomal aberrations have been analysed by means of fluorescent in situ hybridisation (FISH) and chromosome-specific composite DNA probes (chromosome painting). The results obtained show that, under simulated microgravity conditions, the levels of both symmetrical and asymmetrical (dicentrics, rings), the number of cells bearing "complex" aberrations and hence the total numbers of aberrations were significantly elevated at any of the dose-levels assayed, compared to the parallel treatments performed as 1g control ("ground"). Furthermore, the ratio symmetrical:asymmetrical translocations was markedly elevated under simulated microgravity conditions, compared to the findings usually observed under "normal" 1g conditions. On these bases, we are much inclined to believe that simulated microgravity, rather than limiting the resealing of DNA double strand breaks (DSB's) induced by genotoxic agents is influencing in terms of enhancement the misrejoining of DSB's which is actually responsible for the fixation of the original lesions to DNA into chromosomal aberrations. In addition, the possible different misrepair processes leading to the formation of symmetrical and asymmetrical translocations might be differentially influenced by microgravity being the symmetrical translocations significantly more represented.     

数据处理软件与机载数据采集软件的连接

由于飞行测试数据采集和遥测系统引用了能提供动态控制能力的微型计算机,所以软件正日益成为机载数据系统的一个不可缺少的组成部分。通常,软件的重要性总是被机载系统的许多用户所低估,甚至迄今,他们还在担心能否把PCM数据循环图编程到电可编程序只读存储器(EPROM)并将它们插入机载硬件中去。由于较新的可编程序系统使用带有电可擦可编程序只读存储器(E~2PROM)和随机存储器(RAM)的微型计算机来提供实时工程单位(EU)显示和控制能力,因而机载软件就成为整个系统计划的一个中心环节。在这些数据采集系统中,实时数据显示和控制所提出的程序设计要求,使地面站的数据处理软件和飞机上的数据采集软件之间要有软件结构控制和一种软件(固件)连接变得非常重要。本文介绍飞行测试过程中新出现的这类问题的一种解决办法。     

空间辐射对增韧环氧石墨复合材料尺寸稳定性的影响

本文讨论了模拟空间辐射环境对用弹性体增韧环氧石墨复合材料尺寸稳定性的影响。描述了该材料受辐照后(剂量相当于在地球同步轨道上6个月到30年受到的辐照剂量)的响应特性。其结果显示,辐照与环氧基体的相互作用使得复合材料在相当低的总剂量(10~7rads)下即发生脆化。在热交变期间,材料脆化导致热膨胀性能的变化并产生大量的层合板微裂纹。这些性能的变化,限制了这些材料在某些空间飞行器上的使用寿命。     

修补路面用的快硬混凝土

工厂（车间）的楼地面、机场跑道的道面、公路路面、停车库、贮水结构等钢筋砼工程，随着使用年限的增长，其损坏程度也日益严重。而其之修补则需要时间，轻则影响生产，重则波及国计民生。美国新泽西州运输部开发出一种早期强度很高的快硬砼，可使修补（砼养护）的时间为最少。本文仅对此作一简单的介绍。     

Hydrogen in rocks: an energy source for deep microbial communities

To survive in deep subsurface environments, lithotrophic microbial communities require a sustainable energy source such as hydrogen. Though H2 can be produced when water reacts with fresh mineral surfaces and oxidizes ferrous iron, this reaction is unreliable since it depends upon the exposure of fresh rock surfaces via the episodic opening of cracks and fissures. A more reliable and potentially more voluminous H2 source exists in nominally anhydrous minerals of igneous and metamorphic rocks. Our experimental results indicate that H2 molecules can be derived from small amounts of H2O dissolved in minerals in the form of hydroxyl, OH- or O3Si-OH, whenever such minerals crystallized in an H2O-laden environment. Two types of experiments were conducted. Single crystal fracture experiments indicated that hydroxyl pairs undergo an in situ redox conversion to H2 molecules plus peroxy links, O3Si/OO\SiO3. While the peroxy links become part of the mineral structure, the H2 molecules diffused out of the freshly fractured mineral surfaces. If such a mechanism occurred in natural settings, the entire rock column would become a volume source of H2. Crushing experiments to facilitate the outdiffusion of H2 were conducted with common crustal igneous rocks such as granite, andesite, and labradorite. At least 70 nmol of H2/g diffused out of coarsely crushed andesite, equivalent at standard pressure and temperature to 5,000 cm3 of H2/m3 of rock. In the water-saturated, biologically relevant upper portion of the rock column, the diffusion of H2 out of the minerals will be buffered by H2 saturation of the intergranular water film.     

孔、轮廓和表面(十二) 第八章 座标磨削的原理和应用(一)

座标镗床,用于未淬火材料的孔加工是胜任、有效的,但对淬火材料的加工,却无能为力,这是一个急待解决的关键问题。鉴于需要一种能按尺寸进行精密定位和磨孔的手段,摩尔于1940年首先发明了座标磨床。座标磨床是利用座标镗床已有的直线位置控制原理,再加上行星磨削而形成的。由此引     

孔、轮廓和表面(四) 第三章 精度的基础(二)

丝杠或测微螺旋标准——人们希求有一种长度标准,既能用于无级微小增量分度,又能用于测量和位置控制。采用精密螺旋,是这种探索的自然结果。因为只有精密螺旋能够全部满足这种要求。它兼有静态和动态两种测量效     

Production of oxidants by ion irradiation of water/carbon dioxide frozen mixtures

We present new experimental results on the formation of oxidants, such as hydrogen peroxide, ozone, and carbonic acid, under ion irradiation of icy mixtures of water/carbon dioxide at different ratios and temperatures (16 and 80 K). Pure water ice layers and mixtures with carbon dioxide were irradiated by 200 keV He+ ions. We found that the CO(2)/H(2)O ratio progressively decreased to a value of about 0.1, the H(2)O(2) production increased with increasing CO(2) abundance at both 16 and 80 K, and the CO and H(2)CO(3) production increased with increasing CO(2) abundance at 16 K. At 80 K, the synthesis of CO was less efficient because of the high volatility of the molecule that partially sublimed from the target. The production of carbonic acid was connected with the production of CO(3). O(3) was detected only after ion irradiation of CO(2)-rich mixtures. The experimental results are discussed with regard to the relevance they may have in the production of an energy source for a europan or a martian biosphere.