Uncertainties in estimates of the risks of late effects from space radiation.

Methods used to project risks in low-Earth orbit are of questionable merit for exploration missions because of the limited radiobiology data and knowledge of galactic cosmic ray (GCR) heavy ions, which causes estimates of the risk of late effects to be highly uncertain. Risk projections involve a product of many biological and physical factors, each of which has a differential range of uncertainty due to lack of data and knowledge. Using the linear-additivity model for radiation risks, we use Monte-Carlo sampling from subjective uncertainty distributions in each factor to obtain an estimate of the overall uncertainty in risk projections. The resulting methodology is applied to several human space exploration mission scenarios including a deep space outpost and Mars missions of duration of 360, 660, and 1000 days. The major results are the quantification of the uncertainties in current risk estimates, the identification of factors that dominate risk projection uncertainties, and the development of a method to quantify candidate approaches to reduce uncertainties or mitigate risks. The large uncertainties in GCR risk projections lead to probability distributions of risk that mask any potential risk reduction using the "optimization" of shielding materials or configurations. In contrast, the design of shielding optimization approaches for solar particle events and trapped protons can be made at this time and promising technologies can be shown to have merit using our approach. The methods used also make it possible to express risk management objectives in terms of quantitative metrics, e.g., the number of days in space without exceeding a given risk level within well-defined confidence limits.     

Residual chromatin breaks as biodosimetry for cell killing by carbon ions

We have studied the relationship between cell killing and the induction of residual chromatin breaks on various human cell lines and primary cultured cells obtained by biopsy from patients irradiated with either X-rays or heavy-ion beams to identify potential bio-marker of radiosensitivity for radiation-induced cell killing. The carbon-ion beams were accelerated with the Heavy Ion Medical Accelerator in Chiba (HIMAC). Six primary cultures obtained by biopsy from 6 patients with carcinoma of the cervix were irradiated with two different mono-LET beams (LET= 13 keV/μm, 76 keV/μm) and 200kV X rays. Residual chromatin breaks were measured by counting the number of non-rejoining chromatin fragments detected by the premature chromosome condensation (PCC) technique after a 24 hour postirradiation incubation period. The induction rate of residual chromatin breaks per cell per Gy was the highest for 76 keV/μm beams on all of the cells. Our results indicated that cell which was more sensitive to the cell killing was similarly more susceptible to induction of residual chromatin breaks. Furthermore there is a good correlation between these two end points in various cell lines and primary cultured cells. This suggests that the detection of residual chromatin breaks by the PCC technique may be useful as a predictive assay of tumor response to cancer radiotherapy.     

Neurobiological problems in long-term deep space flights.

Future missions in space may involve long-term travel beyond the magnetic field of the Earth, subjecting astronauts to radiation hazards posed by solar flares and galactic cosmic rays, altered gravitation fields and physiological stress. Thus, it is critical to determine if there will be any reversible or irreversible, detrimental neurological effects from this prolonged exposure to space. A question of particular importance focuses on the long-term effects of the space environment on the central nervous system (CNS) neuroplasticity, with the potential acute and/or delayed effects that such perturbations might entail. Although the short-term effects of microgravity on neural control were studied on previous low earth orbit missions, the late consequences of stress in space, microgravity and space radiation have not been addressed sufficiently at the molecular, cellular and tissue levels. The possibility that space flight factors can interact influencing the neuroplastic response in the CNS looms critical issue not only to understand the ontogeny of the CNS and its functional integrity, but also, ultimately the performance of astronauts in extended space forays. The purpose of this paper is to review the neurobiological modifications that occur in the CNS exposed to the space environment, and its potential consequences for extended deep space flight.     

Seasonal-to-interannual variability of chlorophyll-a bloom timing associated with physical forcing in the Gulf of Cádiz

Seasonal-to-interannual variability of the winter-spring bloom in the Gulf of Cádiz, eastern North Atlantic, has been investigated using chlorophyll-a remote sensing (CHL). These data have been obtained from the GlobColour project; the temporal coverage extends from September 1997 to December 2010. In this study we develop a generic quantitative approach for describing the temporal variability in the shape of the winter-spring bloom within a region. Variability in both the timing and magnitude of the bloom in the basin has been evaluated as a function of physical properties in the water column such as Mixed Layer Depth (MLD, GODAS model), sea surface temperature (SST, from AVHRR radiometers), photosynthetically-active radiation (PAR, from ocean color data) and euphotic depth (Z_eu, from ocean color data). The analysis indicated that the timing, size and duration of the phytoplankton bloom in this area are largely controlled by both meteorological and oceanographic conditions at different scales; this means that it is likely to vary widely from one year to another.     

Satellite and ground detection of very dense smoke clouds produced on the islands of the Paraná river delta that affected a large region in Central Argentina

Intense fires were produced on the Paraná river delta islands, Argentina, during most part of 2008, by a combination of an exceptionally dry period and the farmers’ use of a fire land-cleaning technique. In April 2008, those fires significantly affected the nearby regions and their inhabitants, from Rosario city to Buenos Aires mega-city. In this work we present satellite as well as ground Aerosol Optical Depth (AOD) at 550 nm data obtained during the propagation of pollution clouds to the central zone of Argentina. The highest value (1.18) was registered at Buenos Aires by atmospheric remote sensing, using the satellite instrument MODIS/Terra on April 18th 2008 at 10:35 local time (= UT − 3 h). On the same day, ground air quality detectors also measured in this city the highest Total Suspended Particle (TSP) value of the month, 2.02 mg/m³. The AOD(550) daily variation at Rosario Astronomical Observatory, which is located near the Paraná riverside, was derived by combining solar ultraviolet erythemal irradiance data (measured with a YES biometre) with model calculations. On April 25th 2008, from 12:00 to 15:30 local time, a rather high and constant AOD(550) value was registered, with a mean value of (0.90 ± 0.21). Cities located on the side of the Rosario–Buenos Aires highway (San Nicolás, Baradero and San Pedro) were also affected, showing a mean AOD(550) between the Rosario and Buenos Aires values. The particulate matter was collected with gridded samplers placed on the Paraná river islands as well as at the Rosario Observatory. They were analysed with a Scanning Electron Microscope (SEM) and mainly showed a biological origin. Even if normally large particles travel small distances from the source, organic aerosol in the range of 40–100 μm and complex asymmetric structures were registered several kilometres away from the aerosol sources on the islands. Another event of intense UV index attenuation (98.6%) occurred on September 18th 2008, due to very dense smoke clouds that extended over the Rosario area for several hours. The clouds were driven away from the fires by East–northeast and East–southeast winds. The minimum value of this index measured around noon allows to derive a maximum AOD(550)_max = (3.65 ± 0.90) at 12:45 local time. Soot clouds extended over the Paraná river, transporting Burned Biomass Debris (BBD) that deposited on Rosario. In particular, burned leaves and small branches with dimensions of 1–20 cm were collected. The mean (BBD) particles deposited on the ground from 7:00 to 19:00 local time were (0.92 ± 0.20) BBD/(m² h).     

X-ray induced alterations in the differentiation and mineralization potential of murine preosteoblastic cells

To evaluate the effects of ionizing radiation (IR) on murine preosteoblastic cell differentiation, we directed OCT-1 cells to the osteoblastic lineage by treatment with a combination of β-glycerophosphate (β-GP), ascorbic acid (AA), and dexamethasone (Dex). In vitro mineralization was evaluated based on histochemical staining and quantification of the hydroxyapatite content of the extracellular bone matrix. Expression of mRNA encoding Runx2, transforming growth factor β1 (TGF-β1), osteocalcin (OCN), and p21^CDKN1A was analyzed. Exposure to IR reduced the growth rate and diminished cell survival of OCT-1 cells under standard conditions. Notably, calcium content analysis revealed that deposition of mineralized matrix increased significantly under osteogenic conditions after X-ray exposure in a time-dependent manner. In this study, higher radiation doses exert significant overall effects on TGF-β1, OCN, and p21^CDKN1A gene expression, suggesting that gene expression following X-ray treatment is affected in a dose-dependent manner. Additionally, we verified that Runx2 was suppressed within 24 h after irradiation at 2 and 4 Gy. Although further studies are required to verify the molecular mechanism, our observations strongly suggest that treatment with IR markedly alters the differentiation and mineralization process of preosteoblastic cells.     

Ion-acoustic waves in unmagnetized collisionless weakly relativistic plasma of warm-ion and isothermal-electron using time-fractional KdV equation

Collisionless unmagnetized plasma consisting of a mixture of warm ion-fluid and isothermal-electron is considered, assuming that the ion flow velocity has a weak relativistic effect. The reductive perturbation method has been employed to derive the Korteweg–de Vries (KdV) equation for small – but finite-amplitude electrostatic ion-acoustic waves in this plasma. The semi-inverse method and Agrawal’s method lead to the Euler–Lagrange equation that leads to the time fractional KdV equation. The variational-iteration method given by He is used to solve the derived time fractional KdV equation. The calculations show that the fractional order may play the same rule of higher order dissipation in KdV equation to modulate the soliton wave amplitude in the plasma system. The results of the present investigation may be applicable to some plasma environments, such as space-plasmas, laser-plasma interaction, plasma sheet boundary layer of the earth’s magnetosphere, solar atmosphere and interplanetary space.     

Comparison of Antarctic riometer radio wave absorption and THEMIS mission energetic electron fluxes

Simultaneous observations of in situ plasma properties in the tail of the Earth’s magnetosphere and of ground based instruments, lying on the same geomagnetic field lines, have recently proved to yield significant new results. In most cases magnetosphere ionosphere interactions during the night-time northern hemisphere conditions are studied. Here, observations of energetic electrons in the tail of the Earth’s magnetosphere made by the THEMIS mission satellites are compared with auroral radio wave absorption determined by riometers in the Antarctic for sunlit conditions. Days for which satellites and riometers are connected by the same geomagnetic field line are selected using a geomagnetic field model. The six days analysed show clear associations between fluxes and absorptions in some cases. However, these do not necessarily correspond to conjugacy intervals. Hours of positive associations are 1.65 times those for negative associations, all hours and days considered (1.42–3.6 on five days and 0.58 on the other day). These computations are assumed appropriate since the footprints of the satellites used approximately follow corrected geomagnetic parallels for all six days studied. The use of a finer parameterization of geomagnetic models to determine conjugacy may be needed.     

Relativistic electron fluxes and dose rate variations during April–May 2010 geomagnetic disturbances in the R3DR data on ISS

Space radiation has been monitored successfully using the Radiation Risks Radiometer-Dosimeter (R3D) installed at the ESA EXPOSE-R (R3DR) facility outside of the Russian Zvezda module of the International Space Station (ISS) between March 2009 and January 2011. R3DR is a Liulin type spectrometer–dosimeter with a single Si PIN detector 2 cm² of area and 0.3 mm thick. The R3DR instrument accumulated about 2 million measurements of the absorbed dose rate and flux of 10 s resolution. The total external and internal shielding before the detector of R3DR device is 0.41 g cm⁻². The calculated stopping energy of normally incident particles to the detector is 0.78 MeV for electrons and 15.8 MeV for protons. After the Coronal Mass Ejection (CME) at 09:54 UTC on 3 April 2010, a shock was observed at the ACE spacecraft at 0756 UTC on 5 April, which led to a sudden impulse on Earth at 08:26 UTC. Nevertheless, while the magnetic substorms on 5 and 6 of April were moderate; the second largest in history of GOES fluence of electrons with energy >2 MeV was measured. The R3DR data show a relatively small amount of relativistic electrons on 5 April. The maximum dose rate of 2323 μGy day⁻¹ was reached on 7 April; by 9 April, a dose of 6600 μGy was accumulated. By the end of the period on 7 May 2010 a total dose of 11,587 μGy was absorbed. Our data were compared with AE-8 MIN, CRESS and ESA-SEE1 models using SPENVIS and with similar observations on American, Japanese and Russian satellites.     

Fast Probabilistic Particle Identification algorithm using silicon strip detectors

Active detectors used as radiation monitors in space are not usually able to perform Particle Identification (PID). Common techniques need energy loss spectra with high statistics to estimate ion abundances. The ALTEA-space detector system is a set of silicon strip particle telescopes monitoring the radiation environment on board the International Space Station since July 2006 with real-time telemetry capabilities. Its large geometrical factor due to the concurrent use of six detectors permits the acquisition of good energy loss spectra even in a short period of observation. In this paper we present a novel Fast Probabilistic Particle Identification (FPPI) algorithm developed for the ALTEA data analysis in order to perform nuclear identification with low statistics and, with some limitations, also in real time.