Molecular alterations in tumorigenic human bronchial and breast epithelial cells induced by high LET radiation.

Carcinogenesis is a multi-stage process with sequence of genetic events governing the phenotypic expression of a series of transformation steps leading to the development of metastatic cancer. In the present study, immortalized human bronchial (BEP2D) and breast (MCF-10F) cells were irradiated with graded doses of either 150 keV/micrometer alpha particles or 1 GeV/nucleon 56Fe ions. Transformed cells developed through a series of successive steps before becoming tumorigenic in nude mice. Cell fusion studies indicated that radiation-induced tumorigenic phenotype in BEP2D cells could be completely suppressed by fusion with non-tumorigenic BEP2D cells. The differential expressions of known genes between tumorigenic bronchial and breast cells induced by alpha particles and their respective control cultures were compared using cDNA expression array. Among the 11 genes identified to be differentially expressed in BEP2D cells, three (DCC, DNA-PK and p21(CIP1)) were shown to be consistently down-regulated by 2 to 4 fold in all the 5 tumor cell lines examined. In contrast, their expressions in the fusion cell lines were comparable to control BEP2D cells. Similarly, expression levels of a series of genes were found to be altered in a step-wise manner among tumorigenic MCF-10F cells. The results are highly suggestive that functional alterations of these genes may be causally related to the carcinogenic process.     

Ground performance of air conditioning and water recycle system for a Space Plant Box.

Researchers from 5 Japanese universities have developed a plant growth facility (Space Plant Box) for seed to seed experiments under microgravity. The breadboard model of the Space Plant Box was fabricated by assembling subsystems developed for microgravity. The subsystems include air conditioning and water recycle system, air circulation system, water and nutrient delivery system, lighting system and plant monitoring system. The air conditioning and water recycle system is simply composed of a single heat exchanger, two fans and hydrophilic fibrous strings. The strings allow water movement from the cooler fin in the Cooling Box to root supporting materials in the Plant Growth Chamber driven by water potential deficit. Relative humidity in the Plant Growth Chamber can be changed over a wide range by controlling the ratio of latent heat exchange to sensible heat exchange on the cooling fin of the heat exchanger. The transpiration rate was successfully measured by circulating air inside the Plant Growth Chamber only. Most water was recycled and a small amount of water needed to be added from the outside. The simple, air conditioning and water recycle system for the Space Plant Box showed good performance through a barley (Hordeum vulgare L.) growth experiment.     

The effect of gravity on surface temperature and net photosynthetic rate of plant leaves.

To clarify the effects of gravity on heat/gas exchange between plant leaves and the ambient air, the leaf temperatures and net photosynthetic rates of plant leaves were evaluated at 0.01, 1.0, 1.5 and 2.0 G of 20 seconds each during a parabolic airplane flight. Thermal images of leaves were captured using infrared thermography at an air temperature of 26 degrees C, a relative humidity of 15% and an irradiance of 260 W m-2. The net photosynthetic rates were determined by using a chamber method with an infrared gas analyzer at an air temperature of 20 degrees C, a relative humidity of 50% and a photosynthetic photon flux of 0.5 mmol m-2 s-1. The mean leaf temperature increased by 1 degree C and the net photosynthetic rate decreased by 13% with decreasing gravity levels from 1.0 to 0.01 G. The leaf temperature decreased by 0.5 degree C and the net photosynthetic rate increased by 7% with increasing gravity levels from 1.0 to 2.0 G. Heat/gas exchanges between leaves and the ambient air were more retarded at lower gravity levels. A restricted free air convection under microgravity conditions in space would limit plant growth by retarding heat and gas exchanges between leaves and the ambient air.     

Development of thermal sensors and drilling systems for lunar and planetary regoliths

We consider some novel concepts for thermal properties experiments aboard lunar landers or rovers, that may lead to an improved understanding of both the structure of the lunar near surface layers and the lunar thermal history. The new instruments could be developed using the experience and heritage from recently developed systems, like the Rosetta Lander thermal conductivity experiment MUPUS and existing designs used for terrestrial measurements of thermal conductivity. We describe shortly the working principle of such sensors and the main challenges faced when using them in the airless regolith layers of the Moon or other airless bodies. In addition new concepts to create appropriate drill holes for thermal and other measurements in the lunar regolith are discussed.     

He-like Ar xvii triplet observed by RESIK

We present the observations of He-like Ar triplet lines obtained by RESIK spectrometer aboard CORONAS-F. Interpretation of intensity ratios between triplet lines of lower Z elements is known to provide useful diagnostics of plasma conditions within the emitting source. Here, we investigate whether triplet line ratios are useful for interpretation of higher Z element spectra. A high sensitivity, low background and precise absolute calibration of RESIK allow to consider in addition also the continuum contribution. This provides a way to determine the Ar absolute abundance from the observed triplet component ratios. The method is presented and the results are shown for two selected flares. Derived values of Ar absolute abundance for these flares are found to be similar: 2.6 × 10⁻⁶ and 2.9 × 10⁻⁶. They fall in the range between presently accepted Ar photospheric and coronal abundances.     

Study of GNSS-measured ionospheric total electron content variations generated by powerful HF-heating

The purpose of this work is to report the experimental evidences for the influence of perturbations in the electron density in the dayside mid-latitude ionosphere, that are caused by high-frequency heating of the F2 layer, on the GNSS signals. The experiments were carried out at the Sura heater (Radio Physical Research Institute, N. Novgorod). During the sessions of ionospheric heating with different time modulations of the radiated power the rays linking the navigational satellites with the ground receiver intersected the heated region. Variations in the total electron content (TEC) were studied; these variations are proportional to the reduced phases of navigational signals. It is shown that with the square-wave modulation of the radiated power (with periods of 1, 6, 10 and 15 min), perturbations with periods of the main modulation of heating and its harmonics appear in the spectrum of TEC variations. Examples are presented of identification of the heating-induced variations in TEC, including determination of the amplitudes and time characteristics of these variations.     

A model of the effects of heavy ion radiation on human tissue

In heavy ion radiotherapy and space travel humans are exposed to energetic heavy ions (C, Si, Fe and others). This type of irradiation often produces more severe biological effects per unit dose than more common X-rays. A new Monte Carlo model generates a physical space with the complex geometry of human tissue or a cell culture based model of tissue, which is affected by the passage of ionizing radiation. For irradiation, the model relies on a physical code for the ion track structure; for tissues, cellular maps are derived from two- or three-dimensional confocal microscopy images using image segmentation algorithm, which defines cells as pixilated volumes. The model is used to study tissue-specific statistics of direct ion hits and the remote ion action on cells. As an application of the technique, we considered the spatial pattern of apoptotic cells after heavy ion irradiation. The pattern of apoptosis is modeled as a stochastic process, which is defined by the action cross section taken from available experimental data. To characterize the degree of apoptosis, an autocorrelation function that describes the spatial correlation of apoptotic cells is introduced. The values of the autocorrelation function demonstrate the effect of the directionality of the radiation track on the spatial arrangements of inactivated cells in tissue. This effect is intrinsic only to high linear-energy-transfer radiation.     

On the effect of considering more realistic particle shape and mass parameters in MMOD risk assessments

One of the primary mission risks tracked in the development of all spacecraft is that due to micro-meteoroids and orbital debris (MMOD). Both types of particles, especially those larger than 0.1 mm in diameter, contain sufficient kinetic energy due to their combined mass and velocities to cause serious damage to crew members and spacecraft. The process used to assess MMOD risk consists of three elements: environment, damage prediction, and damage tolerance. Orbital debris risk assessments for the Orion vehicle, as well as the Shuttle, Space Station and other satellites use ballistic limit equations (BLEs) that have been developed using high speed impact test data and results from numerical simulations that have used spherical projectiles. However, spheres are not expected to be a common shape for orbital debris; rather, orbital debris fragments might be better represented by other regular or irregular solids. In this paper we examine the general construction of NASA’s current orbital debris (OD) model, explore the potential variations in orbital debris mass and shape that are possible when using particle characteristic length to define particle size (instead of assuming spherical particles), and, considering specifically the Orion vehicle, perform an orbital debris risk sensitivity study taking into account variations in particle mass and shape as noted above. While the results of the work performed for this study are preliminary, they do show that continuing to use aluminum spheres in spacecraft risk assessments could result in an over-design of its MMOD protection systems. In such a case, the spacecraft could be heavier than needed, could cost more than needed, and could cost more to put into orbit than needed. The results obtained in this study also show the need to incorporate effects of mass and shape in mission risk assessment prior to first flight of any spacecraft as well as the need to continue to develop/refine BLEs so that they more accurately reflect the shape and material density variations inherent to the actual debris environment.     

Time-dependent cosmic ray modulation

Time-dependent cosmic ray modulation is calculated over multiple solar cycles using our well established two-dimensional time-dependent modulation model. Results are compared to Voyager 1, Ulysses and IMP cosmic ray observations to establish compatibility. A time-dependence in the diffusion and drift coefficients, implicitly contained in recent expressions derived by , ,  and , is incorporated into the cosmic ray modulation model. This results in calculations which are compatible with spacecraft observations on a global scale over consecutive solar cycles. This approach compares well to the successful compound approach of Ferreira and Potgieter (2004). For both these approaches the magnetic field magnitude, variance of the field and current sheet tilt angle values observed at Earth are transported time-dependently into the outer heliosphere. However, when results are compared to observations for extreme solar maximum, the computed step-like modulation is not as pronounced as observed. This indicates that some additional merging of these structures into more pronounced modulation barriers along the way is needed.