Functional analysis: a system conceptual design tool [andapplication to ATC system]

Functional analysis is a methodology for analyzing the mission and performance requirements of a system, and translating them into discrete activities or tasks which must be performed by the system. Determination of the system's functionality is the first step in obtaining a conceptual view of a system that is to be designed. The methodology for conducting functional analysis is developed, some of the tools used to support the activity are described, including examples to help solidify the concepts presented     

Modelling of genetically engineered microorganisms introduction in closed artificial microcosms.

The possibility of introducing genetically engineered microorganisms (GEM) into simple biotic cycles of laboratory water microcosms was investigated. The survival of the recombinant strain Escherichia coli Z905 (Apr, Lux+) in microcosms depends on the type of model ecosystems. During the absence of algae blooming in the model ecosystem, the part of plasmid-containing cells E. coli decreased fast, and the structure of the plasmid was also modified. In conditions of algae blooming (Ankistrodesmus sp.) an almost total maintenance of plasmid-containing cells was observed in E. coli population. A mathematics model of GEM's behavior in water ecosystems with different level of complexity has been formulated. Mechanisms causing the difference in luminescent exhibition of different species are discussed, and attempts are made to forecast the GEM's behavior in water ecosystems.     

Magnetospheric Models and Trajectory Computations

The calculation of particle trajectories in the Earth's magnetic field has been a subject of interest since the time of Störmer. The fundamental problem is that the trajectory-tracing process involves using mathematical equations that have `no solution in closed form'. This difficulty has forced researchers to use the `brute force' technique of numerical integration of many individual trajectories to ascertain the behavior of trajectory families or groups. As the power of computers has improved over the decades, the numerical integration procedure has grown more tractable and while the problem is still formidable, thousands of trajectories can be computed without the expenditure of excessive resources. As particle trajectories are computed and the characteristics analyzed we can determine the cutoff rigidity of a specific location and viewing direction and direction and deduce the direction in space of various cosmic ray anisotropies. Unfortunately, cutoff rigidities are not simple parameters due to the chaotic behavior of the cosmic-ray trajectories in the cosmic ray penumbral region. As the computational problem becomes more manageable, there is still the issue of the accuracy of the magnetic field models. Over the decades, magnetic field models of increasing complexity have been developed and utilized. The accuracy of trajectory calculations employing contemporary magnetic field models is sufficient that cosmic ray experiments can be designed on the basis of trajectory calculations. However, the Earth's magnetosphere is dynamic and the most widely used magnetospheric models currently available are static. This means that the greatest uncertainly in the application of charged particle trajectories occurs at low energies.     

1. Transport of Mass,Momentum and Energy in Planetary Magnetodisc Regions

The rapid rotation of the gas giant planets, Jupiter and Saturn, leads to the formation of magnetodisc regions in their magnetospheric environments. In these regions, relatively cold plasma is confined towards the equatorial regions, and the magnetic field generated by the azimuthal (ring) current adds to the planetary dipole, forming radially distended field lines near the equatorial plane. The ensuing force balance in the equatorial magnetodisc is strongly influenced by centrifugal stress and by the thermal pressure of hot ion populations, whose thermal energy is large compared to the magnitude of their centrifugal potential energy. The sources of plasma for the Jovian and Kronian magnetospheres are the respective satellites Io (a volcanic moon) and Enceladus (an icy moon). The plasma produced by these sources is globally transported outwards through the respective magnetosphere, and ultimately lost from the system. One of the most studied mechanisms for this transport is flux tube interchange, a plasma instability which displaces mass but does not displace magnetic flux—an important observational constraint for any transport process. Pressure anisotropy is likely to play a role in the loss of plasma from these magnetospheres. This is especially the case for the Jovian system, which can harbour strong parallel pressures at the equatorial segments of rotating, expanding flux tubes, leading to these regions becoming unstable, blowing open and releasing their plasma. Plasma mass loss is also associated with magnetic reconnection events in the magnetotail regions. In this overview, we summarise some important observational and theoretical concepts associated with the production and transport of plasma in giant planet magnetodiscs. We begin by considering aspects of force balance in these systems, and their coupling with the ionospheres of their parent planets. We then describe the role of the interaction between neutral and ionized species, and how it determines the rate at which plasma mass and momentum are added to the magnetodisc. Following this, we describe the observational properties of plasma injections, and the consequent implications for the nature of global plasma transport and magnetodisc stability. The theory of the flux tube interchange instability is reviewed, and the influences of gravity and magnetic curvature on the instability are described. The interaction between simulated interchange plasma structures and Saturn’s moon Titan is discussed, and its relationship to observed periodic phenomena at Saturn is described. Finally, the observation, generation and evolution of plasma waves associated with mass loading in the magnetodisc regions is reviewed.     

Antideuterons as an indirect dark matter signature: Si(Li) detector development and a GAPS balloon mission

The General AntiParticle Spectrometer (GAPS) is a novel approach for indirect dark matter searches that exploits cosmic antideuterons. GAPS complements existing and planned direct dark matter searches as well as other indirect techniques, probing a different and unique region of parameter space in a variety of proposed dark matter models. The GAPS method involves capturing antiparticles into a target material with the subsequent formation of an excited exotic atom. The exotic atom decays with the emission of atomic X-rays and pions from the nuclear annihilation, which uniquely identifies the captured antiparticle. This technique has been verified through the accelerator testing at KEK in 2004 and 2005. The prototype flight is scheduled from Hokkaido, Japan in 2011, preparatory for a long duration balloon flight from the Antarctic in 2014.     

X-ray induced alterations in the differentiation and mineralization potential of murine preosteoblastic cells

To evaluate the effects of ionizing radiation (IR) on murine preosteoblastic cell differentiation, we directed OCT-1 cells to the osteoblastic lineage by treatment with a combination of β-glycerophosphate (β-GP), ascorbic acid (AA), and dexamethasone (Dex). In vitro mineralization was evaluated based on histochemical staining and quantification of the hydroxyapatite content of the extracellular bone matrix. Expression of mRNA encoding Runx2, transforming growth factor β1 (TGF-β1), osteocalcin (OCN), and p21^CDKN1A was analyzed. Exposure to IR reduced the growth rate and diminished cell survival of OCT-1 cells under standard conditions. Notably, calcium content analysis revealed that deposition of mineralized matrix increased significantly under osteogenic conditions after X-ray exposure in a time-dependent manner. In this study, higher radiation doses exert significant overall effects on TGF-β1, OCN, and p21^CDKN1A gene expression, suggesting that gene expression following X-ray treatment is affected in a dose-dependent manner. Additionally, we verified that Runx2 was suppressed within 24 h after irradiation at 2 and 4 Gy. Although further studies are required to verify the molecular mechanism, our observations strongly suggest that treatment with IR markedly alters the differentiation and mineralization process of preosteoblastic cells.