Changes in gravitational force induce alterations in gene expression that can be monitored in the live, developing zebrafish heart.

Little is known about the effect of microgravity on gene expression, particularly in vivo during embryonic development. Using transgenic zebrafish that express the gfp gene under the influence of a beta-actin promoter, we examined the affect of simulated-microgravity on GFP expression in the heart. Zebrafish embryos, at the 18-20 somite-stage, were exposed to simulated-microgravity for 24 hours. The intensity of GFP fluorescence associated with the heart was then determined using fluorescence microscopy. Our measurements indicated that simulated-microgravity induced a 23.9% increase in GFP-associated fluorescence in the heart. In contrast, the caudal notochord showed a 17.5% increase and the embryo as a whole showed only an 8.5% increase in GFP-associated fluorescence. This suggests that there are specific effects on the heart causing the more dramatic increase. These studies indicate that microgravity can influence gene expression and demonstrate the usefulness of this in vivo model of 'reporter-gene' expression for studying the effects of microgravity.     

PC-index fluctuations and intermittency of the magnetospheric dynamics

One minute resolution Polar Cap (PC) index was used for the analysis of magnetospheric dynamics. The 1995–2000 time series analysis revealed that the power spectrum of the PC-index fluctuations is a power law in a wide range of frequencies. However, the obtained exponents differ for low and high frequency regions. The probability distribution functions of the PC-index fluctuations show a strong non-gaussian shape, depending on the time of increment. This indicates that the PC-index exhibits intermittency, previously detected in solar wind and auroral electrojet index fluctuations. The PC-index probability distribution functions were fitted by the functional form proposed by Castaing et al. [Velocity probability density functions of high Reynolds number turbulence. Physica D. 46, 177–200, 1990] to describe intermittency phenomena in ordinary turbulent fluid flows. The agreement between the fitting parameters obtained for the PC index and those reported before for solar wind magnetic field fluctuations is within 30%; which is noticeably less than the difference between the same parameters of solar wind and the AE-index fluctuations. This fact indicates that the PC index reflects the solar wind influence on the high-latitude magnetosphere, especially during the summer.     

The Role of Magnetic Reconnection in CME Acceleration

Observations carried out from the coronagraphs on board space missions (LASCO/SOHO, Solar Maximum and Skylab) and ground-based facilities (HAO/Mauna Loa Observatory) show that coronal mass ejections (CMEs) can be classified into two classes based on their kinematics evolution. These two classes of CMEs are so-called fast and slow CMEs. The fast CME starts with a high initial speed that remains more or less constant; it is also called the constant-speed CME. On the other hand, the slow CME starts with a low initial speed, but shows a gradual acceleration; it is also called the accelerated and slow CME. Low and Zhang [Astrophys. J. 564, L53–L56, 2002] suggested that these two classes of CMEs could be a result of a difference in the initial topology of the magnetic fields associated with the underlying quiescent prominences. A normal prominence magnetic field topology will lead to a fast CME, while an inverse quiescent prominence results in a slow CME, because of the nature of the magnetic reconnection processes. In a recent study given by Wu et al. [Solar Phys. 225, 157–175, 2004], it was shown that an inverse quiescent prominence magnetic topology also could produce a fast CME. In this study, we perform a numerical MHD simulation for CMEs occurring in both normal and inverse quiescent prominence magnetic topology. This study demonstrates three major physical processes responsible for destabilization of these two types of prominence magnetic field topologies that can launch CMEs. These three initiation processes are identical to those used by Wu et al. [Solar Phys. 225, 157–175, 2004]. The simulations show that both fast and slow CMEs can be initiated from these two different types of magnetic topologies. However, the normal quiescent prominence magnetic topology does show the possibility for launching a reconnection island (or secondary O-line) that might be thought of as a “CME’’.     

Top-level modeling of an ALS system utilizing object-oriented techniques.

The possible configuration of an Advanced Life Support (ALS) System capable of supporting human life for long-term space missions continues to evolve as researchers investigate potential technologies and configurations. To facilitate the decision process the development of acceptable, flexible, and dynamic mathematical computer modeling tools capable of system level analysis is desirable. Object-oriented techniques have been adopted to develop a dynamic top-level model of an ALS system.This approach has several advantages; among these, object-oriented abstractions of systems are inherently modular in architecture. Thus, models can initially be somewhat simplistic, while allowing for adjustments and improvements. In addition, by coding the model in Java, the model can be implemented via the World Wide Web, greatly encouraging the utilization of the model. Systems analysis is further enabled with the utilization of a readily available backend database containing information supporting the model. The subsystem models of the ALS system model include Crew, Biomass Production, Waste Processing and Resource Recovery, Food Processing and Nutrition, and the Interconnecting Space. Each subsystem model and an overall model have been developed. Presented here is the procedure utilized to develop the modeling tool, the vision of the modeling tool, and the current focus for each of the subsystem models.     

Escaping near-relativistic electron beams from the solar corona

Processes in the solar corona are prodigious accelerators of energetic ions, and electrons. The angular distribution, composition, and spectra of energetic particles observed near Earth gives information on the acceleration mechanisms. A class of energetic particle observations particularly useful in understanding the solar acceleration is the near-relativistic impulsive beam-like electron events. During five years of operation the Advanced Composition Explorer (ACE) has measured well over 400 electron events. Approximately 25% of these electron events are impulsive beam-like events that are released onto interplanetary field lines predominantly from western solar longitudes. We extend our initial 3 year study during the rise to solar maximum (Haggerty and Roelof, 2002; Simnett et al., 2002) to a five year statistical analysis of these beam-like energetic electron events in relationship to optical flares, microwave emission, soft X-ray emission, metric and decametric type-III radio bursts, and coronal mass ejections.     

Parathyroid hormone-related protein is a gravisensor in lung and bone cell biology.

Parathyroid Hormone-related Protein (PTHrP) has been shown to be essential for the development and homeostatic regulation of lung and bone. Since both lung and bone structure and function are affected by microgravity, we hypothesized that 0 x g down-regulates PTHrP signaling. To test this hypothesis, we suspended lung and bone cells in the simulated microgravity environment of a Rotating Wall Vessel Bioreactor, which simulates microgravity, for up to 72 hours. During the first 8 hours of exposure to simulated 0 x g, PTHrP expression fell precipitously, decreasing by 80-90%; during the subsequent 64 hours, PTHrP expression remained at this newly established level of expression. PTHrP production decreased from 12 pg/ml/hour to 1 pg/ml/hour in culture medium from microgravity-exposed cells. The cells were then recultured at unit gravity for 24 hours, and PTHrP expression and production returned to normal levels. Based on these findings, we have obtained bones from rats flown in space for 2 weeks (Mission STS-58, SL-2). Analysis of PTHrP expression by femurs and tibias from these animals (n=5) revealed that PTHrP expression was 60% lower than in bones from control ground-based rats. Interestingly, there were no differences in PTHrP expression by parietal bone from space-exposed versus ground-based animals, indicating that the effect of weightlessness on PTHrP expression is due to the unweighting of weight-bearing bones. This finding is consistent with other studies of microgravity-induced osteoporosis. The loss of the PTHrP signaling mechanism may be corrected using chemical agents that up-regulate this pathway. In conclusion, PTHrP represents a stretch-sensitive paracrine signaling mechanism that may sense gravity.     

Transient Performance of Gyro in Spinning Spacecraft

Transient performance of a single-axis rate gyroscope mounted in a spacecraft which is spinning about the spin axis of the gyro is presented. Analytical expressions for various time-domain and frequency-domain specifications as functions of the spin rate of the vehicle are obtained. Numerical results are presented which are useful in selecting the gyro parameters if it is to be used for the measurements of the angular velocity in spinning space vehicles.     

Spending without results: lessons from the space station program

The US spent all of the funds originally estimated for the initial development of its orbital space station without producing any significant amount of flight hardware. This article shows how a project with large design costs and significant “non-prime” outlays can quickly deplete program funds. The authors recount the way in which budgetary politics, congressional micro-management, and technological risk conspired to produce this result.     

Evolution of binaries with chemically peculiar primaries

The chemically peculiar (CP) stars are classified into subgroups based on the type of peculiarities. A significant fraction of these are known to be binaries. The faster evolution of the massive component leads to a white dwarf or a neutron star. Further evolution of the binary is analysed taking into consideration, the orbital parameters, effect of magnetic field, spectroscopic peculiarities and finally the statistics of CP binaries and Low Mass X-ray Binaries (LMXB).

The possible consequences of the evolution to lead to the formation of Magnetic Cataclysmic Variables (MCV) and LMXB are discussed.     

The Soviet Union's GLONASS Navigation Satellites

GLONASS (Global Orbiting Navigation Satellite System) is the most recent satellite navigation system developed by the Soviet Union and currently in the pre-operational stage. Obvious parallels exist between GLONASS and the NAVSTAR Global Positioning System (GPS) developed in the United States and also, at present, in a pre-operational phase. In the progress towards operational status, the launch capability for NAVSTAR satellites has been seriously affected by the recent failure of the Space Shuttle Challenger, clearly increasing the prospects of GLONASS reaching operational status first. It is therefore the main purpose of this paper to discuss certain aspects of the GLONASS satellite navigation system, in particular its orbital features and radio-frequency signal characteristics. Comparisons with NAVSTAR are inevitable and for this reason, the paper begins with a brief resume of relevant features of the NAVSTAR GPS system for later reference. The main section of the paper then deals with orbital behaviour, radio frequency signal structure and channelisation using NAVSTAR as a reference point for discussion.