Studies on precellular evolution: the encapsulation of polyribonucleotides by liposomes.

Liposomes are 5 to 50 micron vesicles with an internal aqueous environment, whose amphiphilic lipidic components self-assemble into systems with at least one double-layered membrane. Liposomes have been suggested as possible models of precellular systems formed in the early Archean Earth from lipids of non-enzymatic origin. Since it is generally accepted that RNA molecules preceded double-stranded DNA molecules as genetic material, we have studied the encapsulation of polyribonucleotides within liposomes made from dipalmitoyl phosphatidylcholine, and from egg yolk phosphatidylcholine to which cholesterol was added in some cases. The liposomes were prepared under anoxic conditions following the reverse phase evaporation method described by Szoka and Papahadjopoulos. Quantitative determinations show that approximately 50% of the available lipids form liposomes, and that up to 5% of the polyribonucleotides can be entrapped by them. We have also studied the encapsulation of polyribonucleotides in the presence of 1) urea and cyanamide, two non-electrolytes that have been used as prebiotic condensing agents, and 2) of Zn++ and Pb++, two cations employed in the non-enzymatic template-directed synthesis of polyribonucleotides from activated nucleotides.     

Perception of gravity by plants.

Physical principles can be used to predict some features about the gravity perception system in plants. The nature of the system has made it rather elusive, so this approach represents an additional source of information to help find it. For a gravitational stimulus to be detected, two masses must move relative to each other in a manner which causes a significant amount of work to be done on a receptor. Relative to cellular dimensions, the masses must be large, be dense and move noticeable distances. The main sources of noise are thermal motion and flexing of the plant tissue. Some new models for the function of amyloplasts as statoliths are presented.     

Synaptic plasticity and gravity: ultrastructural, biochemical and physico-chemical fundamentals.

On the basis of quantitative disturbances of the swimming behaviour of aquatic vertebrates ("loop-swimming" in fish and frog larvae) following long-term hyper-g-exposure the question was raised whether or not and to what extent changes in the gravitational vector might influence the CNS at the cellular level. Therefore, by means of histological, histochemical and biochemical analyses the effect of 2-4 x g for 9 days on the gross morphology of the fish brain, and on different neuronal enzymes was investigated. In order to enable a more precise analysis in future-microgravity-experiments of any gravity-related effects on the neuronal synapses within the gravity-perceptive integration centers differentiated electron-microscopical and electronspectroscopical techniques have been developed to accomplish an ultrastructural localization of calcium, a high-affinity Ca2(+)-ATPase, creatine kinase and cytochrome oxidase. In hyper-g animals vs. 1-g controls, a reduction of total brain volume (15%), a decrease in creatine kinase activity (20%), a local increase in cytochrome oxidase activity, but no differences in Ca2+/Mg(2+)-ATPase activities were observed. Ultrastructural peculiarities of synaptic contact formation in gravity-related integration centers (Nucleus magnocellularis) were found. These results are discussed on the basis of a direct effect of hyper-gravity not only on the gravity-sensitive neuronal integration centers but possibly also on the physico-chemical properties of the lipid bilayer of neuronal membranes in general.     

The Search-Coil Magnetometer for MMS

The tri-axial search-coil magnetometer (SCM) belongs to the FIELDS instrumentation suite on the Magnetospheric Multiscale (MMS) mission (Torbert et al. in Space Sci. Rev. (2014), this issue). It provides the three magnetic components of the waves from 1 Hz to 6 kHz in particular in the key regions of the Earth’s magnetosphere namely the subsolar region and the magnetotail. Magnetospheric plasmas being collisionless, such a measurement is crucial as the electromagnetic waves are thought to provide a way to ensure the conversion from magnetic to thermal and kinetic energies allowing local or global reconfigurations of the Earth’s magnetic field. The analog waveforms provided by the SCM are digitized and processed inside the digital signal processor (DSP), within the Central Electronics Box (CEB), together with the electric field data provided by the spin-plane double probe (SDP) and the axial double probe (ADP). On-board calibration signal provided by DSP allows the verification of the SCM transfer function once per orbit. Magnetic waveforms and on-board spectra computed by DSP are available at different time resolution depending on the selected mode. The SCM design is described in details as well as the different steps of the ground and in-flight calibrations.     

REMS: The Environmental Sensor Suite for the Mars Science Laboratory Rover

The Rover Environmental Monitoring Station (REMS) will investigate environmental factors directly tied to current habitability at the Martian surface during the Mars Science Laboratory (MSL) mission. Three major habitability factors are addressed by REMS: the thermal environment, ultraviolet irradiation, and water cycling. The thermal environment is determined by a mixture of processes, chief amongst these being the meteorological. Accordingly, the REMS sensors have been designed to record air and ground temperatures, pressure, relative humidity, wind speed in the horizontal and vertical directions, as well as ultraviolet radiation in different bands. These sensors are distributed over the rover in four places: two booms located on the MSL Remote Sensing Mast, the ultraviolet sensor on the rover deck, and the pressure sensor inside the rover body. Typical daily REMS observations will collect 180 minutes of data from all sensors simultaneously (arranged in 5 minute hourly samples plus 60 additional minutes taken at times to be decided during the course of the mission). REMS will add significantly to the environmental record collected by prior missions through the range of simultaneous observations including water vapor; the ability to take measurements routinely through the night; the intended minimum of one Martian year of observations; and the first measurement of surface UV irradiation. In this paper, we describe the scientific potential of REMS measurements and describe in detail the sensors that constitute REMS and the calibration procedures.     

Trends in the Neutral and Ionized Upper Atmosphere

This article reviews our knowledge of long-term changes and trends in the upper atmosphere and ionosphere. These changes are part of complex and comprehensive pattern of long-term trends in the Earth’s atmosphere. They also have practical impact. For example, decreasing thermospheric density causes the lifetime of orbiting space debris to increase, which is becoming a significant threat to important satellite technologies. Since the first paper on upper atmosphere trends was published in 1989, our knowledge has progressed considerably. Anthropogenic emissions of greenhouse gases affect the whole atmosphere, not only the troposphere. They cause warming in the troposphere but cooling in the upper atmosphere. Greenhouse gases such as carbon dioxide are not the only driver of long-term changes and trends in the upper atmosphere and ionosphere. Anthropogenic changes of stratospheric ozone, long-term changes of geomagnetic and solar activity, and other drivers play a role as well, although greenhouse gases appear to be the main driver of long-term trends. This makes the pattern of trends more complex and variable. A?consistent, although incomplete, scenario of trends in the upper atmosphere and ionosphere is presented. Trends in F2-region ionosphere parameters, in mesosphere-lower thermosphere dynamics, and in noctilucent or polar mesospheric clouds, are discussed in more detail. Advances in observational and theoretical analysis have explained some previous discrepancies in this global trend scenario. An important role in trend investigations is played by model simulations, which facilitate understanding of the mechanisms behind the observed trends.     

Simulations of absorbed dose on the phantom surface of MATROSHKA-R experiment at the ISS

The health risks associated with exposure to various components of space radiation are of great concern when planning manned long-term interplanetary missions, such as future missions to Mars. Since it is not possible to measure the radiation environment inside of human organs in deep space, simulations based on radiation transport/interaction codes coupled to phantoms of tissue equivalent materials are used. However, the calculated results depend on the models used in the codes, and it is therefore necessary to verify their validity by comparison with measured data. The goal of this paper is to compare absorbed doses obtained in the MATROSHKA-R experiment performed at the International Space Station (ISS) with simulations performed with the three-dimensional Monte Carlo Particle and Heavy-Ion Transport code System (PHITS). The absorbed dose was measured using passive detectors (packages of thermoluminescent and plastic nuclear track detectors) placed on the surface of the spherical tissue equivalent phantom MATROSHKA-R, which was exposed aboard the ISS in the Service Zvezda Module from December 2005 to September 2006. The data calculated by PHITS assuming an ISS shielding of 3 g/cm² and 5 g/cm² aluminum mass thickness were in good agreement with the measurements. Using a simplified geometrical model of the ISS, the influence of variations in altitude and wall mass thickness of the ISS on the calculated absorbed dose was estimated. The uncertainties of the calculated data are also discussed; the relative expanded uncertainty of absorbed dose in phantom was estimated to be 44% at a 95% confidence level.     

The Mars Science Laboratory Organic Check Material

Mars Science Laboratory’s Curiosity rover carries a set of five external verification standards in hermetically sealed containers that can be sampled as would be a Martian rock, by drilling and then portioning into the solid sample inlet of the Sample Analysis at Mars (SAM) suite. Each organic check material (OCM) canister contains a porous ceramic solid, which has been doped with a fluorinated hydrocarbon marker that can be detected by SAM. The purpose of the OCM is to serve as a verification tool for the organic cleanliness of those parts of the sample chain that cannot be cleaned other than by dilution, i.e., repeated sampling of Martian rock. SAM possesses internal calibrants for verification of both its performance and its internal cleanliness, and the OCM is not used for that purpose. Each OCM unit is designed for one use only, and the choice to do so will be made by the project science group (PSG).     

Selection of the Mars Science Laboratory Landing Site

The selection of Gale crater as the Mars Science Laboratory landing site took over five years, involved broad participation of the science community via five open workshops, and narrowed an initial >50 sites (25 by 20?km) to four finalists (Eberswalde, Gale, Holden and Mawrth) based on science and safety. Engineering constraints important to the selection included: (1)?latitude (±30°) for thermal management of the rover and instruments, (2)?elevation (<?1?km) for sufficient atmosphere to slow the spacecraft, (3)?relief of <100–130?m at baselines of 1–1000?m for control authority and sufficient fuel during powered descent, (4)?slopes of <30° at baselines of 2–5?m for rover stability at touchdown, (5)?moderate rock abundance to avoid impacting the belly pan during touchdown, and (6)?a?radar-reflective, load-bearing, and trafficable surface that is safe for landing and roving and not dominated by fine-grained dust. Science criteria important for the selection include the ability to assess past habitable environments, which include diversity, context, and biosignature (including organics) preservation. Sites were evaluated in detail using targeted data from instruments on all active orbiters, and especially Mars Reconnaissance Orbiter. All of the final four sites have layered sedimentary rocks with spectral evidence for phyllosilicates that clearly address the science objectives of the mission. Sophisticated entry, descent and landing simulations that include detailed information on all of the engineering constraints indicate all of the final four sites are safe for landing. Evaluation of the traversabilty of the landing sites and target “go to” areas outside of the ellipse using slope and material properties information indicates that all are trafficable and “go to” sites can be accessed within the lifetime of the mission. In the final selection, Gale crater was favored over Eberswalde based on its greater diversity and potential habitability.     

Properties of Ground Level Enhancement Events and the Associated Solar Eruptions During Solar Cycle 23

Solar cycle 23 witnessed the most complete set of observations of coronal mass ejections (CMEs) associated with the Ground Level Enhancement (GLE) events. We present an overview of the observed properties of the GLEs and those of the two associated phenomena, viz., flares and CMEs, both being potential sources of particle acceleration. Although we do not find a striking correlation between the GLE intensity and the parameters of flares and CMEs, the solar eruptions are very intense involving X-class flares and extreme CME speeds (average ～2000?km/s). An M7.1 flare and a 1200?km/s CME are the weakest events in the list of 16 GLE events. Most (80?%) of the CMEs are full halos with the three non-halos having widths in the range 167 to 212?degrees. The active regions in which the GLE events originate are generally large: 1290?msh (median 1010?msh) compared to 934?msh (median: 790?msh) for SEP-producing active regions. For accurate estimation of the CME height at the time of metric type?II onset and GLE particle release, we estimated the initial acceleration of the CMEs using flare and CME observations. The initial acceleration of GLE-associated CMEs is much larger (by a factor of 2) than that of ordinary CMEs (2.3?km/s² vs. 1?km/s²). We confirmed the initial acceleration for two events for which CME measurements are available in the inner corona. The GLE particle release is delayed with respect to the onset of all electromagnetic signatures of the eruptions: type?II bursts, low frequency type?III bursts, soft X-ray flares and CMEs. The presence of metric type?II radio bursts some 17?min (median: 16?min; range: 3 to 48?min) before the GLE onset indicates shock formation well before the particle release. The release of GLE particles occurs when the CMEs reach an average height of ～3.09?R _s (median: 3.18?R _s ; range: 1.71 to 4.01?R _s ) for well-connected events (source longitude in the range W20–W90). For poorly connected events, the average CME height at GLE particle release is ～66?% larger (mean: 5.18?R _s ; median: 4.61?R _s ; range: 2.75–8.49?R _s ). The longitudinal dependence is consistent with shock accelerations because the shocks from poorly connected events need to expand more to cross the field lines connecting to an Earth observer. On the other hand, the CME height at metric type?II burst onset has no longitudinal dependence because electromagnetic signals do not require magnetic connectivity to the observer. For several events, the GLE particle release is very close to the time of first appearance of the CME in the coronagraphic field of view, so we independently confirmed the CME height at particle release. The CME height at metric type?II burst onset is in the narrow range 1.29 to 1.8?R _s , with mean and median values of 1.53 and 1.47?R _s . The CME heights at metric type?II burst onset and GLE particle release correspond to the minimum and maximum in the Alfvén speed profile. The increase in CME speed between these two heights suggests an increase in Alfvénic Mach number from?2 to?3. The CME heights at GLE particle release are in good agreement with those obtained from the velocity dispersion analysis (Reames in Astrophys. J. 693:812, 2009a; Astrophys. J. 706:844, 2009b) including the source longitude dependence. We also discuss the implications of the delay of GLE particle release with respect to complex type?III bursts by ～18?min (median: 16?in; range: 2 to 44?min) for the flare acceleration mechanism. A?similar analysis is also performed on the delay of particle release relative to the hard X-ray emission.