Composition and physical properties of starch in microgravity-grown plants.

The effect of spaceflight on starch development in soybean (Glycine max L., BRIC-03) and potato (Solanum tuberosum, Astroculture-05) was compared with ground controls by biophysical and biochemical measurements. Starch grains from plants from both flights were on average 20-50% smaller in diameter than ground controls. The ratio delta X/delta rho (delta X --difference of magnetic susceptibilities, delta rho--difference of densities between starch and water) of starch grains was ca. 15% and 4% higher for space-grown soybean cotyledons and potato tubers, respectively, than in corresponding ground controls. Since the densities of particles were similar for all samples (1.36 to 1.38 g/cm3), the observed difference in delta X/delta rho was due to different magnetic susceptibilities and indicates modified composition of starch grains. In starch preparations from soybean cotyledons (BRIC-03) subjected to controlled enzymatic degradation with alpha-amylase for 24 hours, 77 +/- 6% of the starch from the flight cotyledons was degraded compared to 58 +/- 12% in ground controls. The amylose content in starch was also higher in space-grown tissues. The good correlation between the amylose content and delta X/delta rho suggests, that the magnetic susceptibility of starch grains is related to their amylose content. Since the seedlings from the BRIC-03 experiment showed elevated post-flight ethylene levels, material from another flight experiment (GENEX) which had normal levels of ethylene was examined and showed no difference to ground controls in size distribution, density, delta X/delta rho and amylose content. Therefore the role of ethylene appears to be more important for changes in starch metabolism than microgravity.     

First radiobiological results of LDEF-1 experiment A0015 with Arabidopsis seed embryos and Sordaria fungus spores.

This article highlights the first results of investigations on the general vitality and damage endpoints caused by cosmic ionizing radiation in dry, dormant plant seeds of the crucifer plant Arabidopsis thaliana (L.) Heynh. and the ascomycete Sordaria fimicola after 69 month stay in space. Wild-type and mutant gene marker lines were included in Free Flyer Biostack containers and exposed on earth and side tray of the LDEF-1 satellite. The damage in biological endpoints observed in the seeds increased in the side tray sample compared to the earth tray sample. For the ascospores we found different effects depending on the biological endpoints investigated for both expositions.     

Potential vascular damage from radiation in the space environment.

Cultured endothelial cells of blood vessels have a Do of 2 Gy for X-rays. A dose of 0.5 Gy of X-rays has an acute effect on vessel diameter. The vessels may show other acute effects such as change in permeability including a change in the blood brain barrier. Changes occurring from late effects of chronic exposure in vascular architecture include telangiectasia and decrease in vascular density. Changes in the perivascular connective tissue particularly collagen may play a role in these changes. After charged particle exposure of 15 and 30 Gy, radiation changes in the blood brain barrier and vascular changes are noted in the nervous system. These long term changes are recorded by PET, MRI, and CT imaging. Chronic exposure to alpha particles causes vascular damage in compact bone resulting in bone infarcts. Using tandem scanning confocal microscopy in-situ imaging of the capillaries and collagen of the papillary dermis provides a non-invasive method of serial recording of changes in irradiated microvasculature.     

Soybean cotyledon starch metabolism is sensitive to altered gravity conditions.

We have demonstrated that etiolated soybean seedlings grown under the altered gravity conditions of clinorotation (1 rpm) and centrifugation (5xg) exhibit changes in starch metabolism. Cotyledon starch concentration was lower (-28%) in clinorotated plants and higher (+24%) in centrifuged plants than in vertical control plants. The activity of ADP-glucose pyrophosphorylase in the cotyledons was affected in a similar way, i.e. lower (-37%) in the clinorotated plants and higher (+22%) in the centrifuged plants. Other starch metabolic enzyme activities, starch synthase, starch phosphorylase and total hydrolase were not affected by the altered gravity treatments. We conclude that the observed changes in starch concentrations were primarily due to gravity-mediated differences in ADP-glucose pyrophosphorylase activity.     

Influence of gravity on the circadian timing system.

The circadian timing system (CTS) is responsible for daily temporal coordination of physiological and behavioral functions both internally and with the external environment. Experiments in altered gravitational environments have revealed changes in circadian rhythms of species ranging from fungi to primates. The altered gravitational environments examined included both the microgravity environment of spaceflight and hyperdynamic environments produced by centrifugation. Acute exposure to altered gravitational environments changed homeostatic parameters such as body temperature. These changes were time of day dependent. Exposure to gravitational alterations of relatively short duration produced changes in both the homeostatic level and the amplitude of circadian rhythms. Chronic exposure to a non-earth level of gravity resulted in changes in the period of the expressed rhythms as well as in the phase relationships between the rhythms and between the rhythms and the external environment. In addition, alterations in gravity appeared to act as a time cue for the CTS. Altered gravity also affected the sensitivity of the pacemaker to other aspects of the environment (i.e., light) and to shifts of time cues. Taken together, these studies lead to the conclusion that the CTS is indeed sensitive to gravity and its alterations. This finding has implications for both basic biology and space medicine.     

A modified Gaussian sum approach to estimation of non-Gaussiansignals

A Gaussian sum estimation algorithm has previously been developed to deal with noise processes that are non-Gaussian. Inherent in this algorithm is a serious growing memory problem that causes the number of terms in the Gaussian sum to increase exponentially at each iteration. A modified Gaussian sum estimation algorithm using an adaptive filter is developed that avoids the growing memory problem of the previous algorithm while providing effective state estimation. The adaptive filter is comprised of a fixed set of estimators operating in parallel with each individual estimate possessing its own corresponding weighting term. A simulation example illustrates the new non-Gaussian estimation technique     

Mutual coupling effects and their reduction in wideband directionof arrival estimation

The performance of multiple signal classification (MUSIC) algorithm using three different inputs over a wideband of frequencies is considered. These inputs are: 1) ideal voltages, 2) actual voltages which include coupling effects and are obtained with the method or moments, and 3) corrected voltages which are obtained from the actual voltages so that the mutual coupling effects are removed. Linear arrays of dipoles, sleeve dipoles, and spiral antennas are considered over 200 MHz to 400 MHz band     

Fusion of sensors with dissimilar measurement/tracking accuracies

The case of data fusion of sensors dissimilar in their measurement/tracking errors is considered. It is shown that the fused track performance is similar whether the sensor data are fused at the track level or at the measurement level. The case of a cluster of targets, resolved by one sensor but not the other, is also considered. Under certain conditions the fused track may perform worse than the worst of the sensors. A remedy to this problem through modifications of the association algorithm is presented     

Reconnection during substorms and solar flares

The patterns of reconnection in the Earth magnetotail and in the solar corona above the active region are presented. The electric field and field-aligned currents (FAC) generation in the current sheet are discussed.     

Interaction of shocks with a current sheet and reconnection in the solar corona

Interaction of shocks with a current sheet is investigated within a 2D MHD model based on an improved FCT numerical scheme. Basic parameters of the problem are chosen to correspond to situations in the solar corona with low plasma β and moderate shock strength. Slow and fast MHD shocks are introduced with shock normal parallel to magnetic field lines. The interaction with the current sheet causes distortion of the shock front and this distorts the magnetic field lines and generates electric current. Large current densities are generated especially when the fast MHD shock becomes the intermediate MHD shock at the current sheet. Then peak values of the current density are about 3–4 times larger than the initial undisturbed values in the current sheet.