Molecular alterations in tumorigenic human bronchial and breast epithelial cells induced by high LET radiation.

Carcinogenesis is a multi-stage process with sequence of genetic events governing the phenotypic expression of a series of transformation steps leading to the development of metastatic cancer. In the present study, immortalized human bronchial (BEP2D) and breast (MCF-10F) cells were irradiated with graded doses of either 150 keV/micrometer alpha particles or 1 GeV/nucleon 56Fe ions. Transformed cells developed through a series of successive steps before becoming tumorigenic in nude mice. Cell fusion studies indicated that radiation-induced tumorigenic phenotype in BEP2D cells could be completely suppressed by fusion with non-tumorigenic BEP2D cells. The differential expressions of known genes between tumorigenic bronchial and breast cells induced by alpha particles and their respective control cultures were compared using cDNA expression array. Among the 11 genes identified to be differentially expressed in BEP2D cells, three (DCC, DNA-PK and p21(CIP1)) were shown to be consistently down-regulated by 2 to 4 fold in all the 5 tumor cell lines examined. In contrast, their expressions in the fusion cell lines were comparable to control BEP2D cells. Similarly, expression levels of a series of genes were found to be altered in a step-wise manner among tumorigenic MCF-10F cells. The results are highly suggestive that functional alterations of these genes may be causally related to the carcinogenic process.     

Design of the TRIO system-on-chip for aerospace

Several design and testing aspects of the TRIO smart sensor data acquisition chip, developed by JHU/APL for NASA spacecraft applications are presented. TRIO includes a 10 bit self-corrected analog-to-digital converter (ADC), 16/32 analog inputs, a front end multiplexer with selectable aquisition time, a current source, memory, serial and parallel bus, and control logic. So far TRIO is used in many missions including Contour, Messenger, Stereo, Pluto, and the generic JPL X2000 spacecraft bus.     

Critical issues in connection with human missions to Mars: protection of and from the Martian environment.

Human missions to Mars are planned to happen within this century. Activities associated therewith will interact with the environment of Mars in two reciprocal ways: (i) the mission needs to be protected from the natural environmental elements that can be harmful to human health, the equipment or to their operations; (ii) the specific natural environment of Mars should be protected so that it retains its value for scientific and other purposes. The following environmental elements need to be considered in order to protect humans and the equipment on the planetary surface: (i) cosmic ionizing radiation, (ii) solar particle events; (iii) solar ultraviolet radiation; (iv) reduced gravity; (v) thin atmosphere; (vi) extremes in temperatures and their fluctuations; and (vii) surface dust. In order to protect the planetary environment, the requirements for planetary protection as adopted by COSPAR for lander missions need to be revised in view of human presence on the planet. Landers carrying equipment for exobiological investigations require special consideration to reduce contamination by terrestrial microorganisms and organic matter to the greatest feasible extent. Records of human activities on the planet's surface should be maintained in sufficient detail that future scientific experimenters can determine whether environmental modifications have resulted from explorations.     

The importance of space policy teaching in communicating space activities to society

The governments’ priority and budgets for space activities are steadily decreasing and the importance of space activities is not any longer reaching the front pages of the newspaper, as in the 1960s. On the other hand in Europe the people, at large, have shown an important interest and support for space activities. A contribution to bridge the gap between decreasing funding and important support of citizen can come from teaching space policy in universities as well as in special workshops for government, industrial and military circles. The paper will outline a course that fulfils this goal.     

Tunnel/predictor display with two-axis control coordination and simplification

A longitudinal-lateral control coordination and simplification concept is considered for a tunnel/predictor display, which presents the command flight path and a specified future aircraft position in a 3-dimensional format to the pilot, for improving flight path control. A basic element of the proposed concept is the generation of common longitudinal-lateral transfer characteristics of the predictor-aircraft system for the crossover frequency region. Control coordination is supported by a dynamics similarity between the longitudinal and the lateral response characteristics of the predictor position in closed-loop pilot control actions. The control coordination and simplification concept is considered to support the pilot in manual flight path control, directed at an enhancement of his control performance and at a reduction of his workload. Results from pilot-in-the-loop simulation experiments support the theoretical findings and show the effectiveness of the proposed predictor concept with control coordination.     

Modeling stress strain state of thin shells with a degenerating domain

We consider a possibility to apply the method of analyzing the complex-shaped shells, proposed in [1], for determining the stress strain state of thin shells with a degenerating domain. The results of numerical calculations are presented.     

The finite element approximation of vector fields in curvilinear coordinates

We demonstrate that it is possible to express each component of the displacement vector for the interior point of the finite element (FE) through all components of nodal unknowns in curvilinear coordinates. The effectiveness of the valid technique of vector approximation for displacement fields has been verified on an example.     

Scientific Planning and Commanding of the Rosetta Payload

ESA’s Rosetta mission was launched in March 2004 and is on its way to comet 67P/Churyumov-Gerasimenko, where it is scheduled to arrive in summer 2014. It comprises a payload of 12 scientific instruments and a Lander. All instruments are provided by Principal Investigators, which are responsible for their operations. As for most ESA science missions, the ground segment of the mission consists of a Mission Operations Centre (MOC) and a Science Operations Centre (SOC). While the MOC is responsible for all spacecraft-related aspects and the final uplink of all command timelines to the spacecraft, the scientific operations of the instruments and the collection of the data and ingestion into the Planetary Science Archive are coordinated by the SOC. This paper focuses on the tasks of the SOC and in particular on the methodology and constraints to convert the scientific goals of the Rosetta mission to operational timelines.     

Stochastic optimal control guidance law with bounded acceleration

A novel stochastic optimal control guidance law is presented for a missile with bounded acceleration. The nonlinear optimal guidance law (NOGL) is obtained by numerically solving the stochastic optimization problem. Since the certainty equivalence principle is not valid in the investigated problem, the resulting NOGL depends on the conditional probability density function of the estimated states. It is shown that the NOGL is also nonlinear in the estimated zero effort miss distance, and that the probability density function of the miss distance is non-Gaussian. The dependence of the new guidance law on the acceleration limit is investigated and it is shown that only for an extremely large acceleration limit does the proposed guidance law degenerate to the classical optimal linear one