应用绿色半导体量子点进行乳腺癌原位成像

乳腺癌是癌症中引起妇女死亡的首要因素。本文采用绿色量子点作为荧光显色材料，建立了一种用于早期乳腺癌诊断的原位成像技术。利用生物素标记的抗HER2／neu抗体与乳腺癌细胞表面表达的HER2结合的特点，然后用偶联绿色量子点的链霉亲和素作为荧光检测标记物，与HER2抗原抗体复合物中的生物素发生特异结合，检测出乳腺癌细胞的存在。结果表明，在荧光显微镜下用绿色量子点原位显色的乳腺癌细胞，相对有机染料组显色亮度增强，故检测灵敏度有所提高。     

Gravi- and photostimuli in moss protonema growth movements.

Moss protonemal growth direction is controlled by at least three factors, photo-, gravi- and autotropism. It is possible to experimentally separate these factors and to control selectively their morphological appearance. In darkness protonema grow negatively gravitropically, and unilateral illumination initiated positive phototropism. Red light suppressed auto- and gravitropism, blue light suppressed only gravitropism. Green light allowed both gravi- and autotropism. The effect of light on gravitropism might involve changes in starch synthesis.     

Multi-channel data structure and real-time compression algorithm research

The development of space telemetry technology has brought forward the need for large capacity memory of any solid-state recorder; data compression therefore, becomes more and more important. The compression feasibility and potentiality of telemetry data are examined by analyzing the statistical characteristics of actual telemetry data recovered from recorders. Aiming at the disadvantage of present data formats in data compression for multi-channel telemetry data acquisition systems, this introduces a data packet structure, and a real-time compression algorithm for low complex hardware design. The principles and implementations of data package compression are described. Simulation results show that this technology can meet the requirements of multi-channel real-time data compression with a high compression ratio and a fast compression speed, which possesses great application value.     

Diagnostics of equatorial and low latitude ionosphere by TEC mapping over Brazil

The total electron content (TEC) in the equatorial and low-latitude ionosphere over Brazil was monitored in two dimensions by using 2011 data from the ground-based global navigation satellite system (GNSS) receiver network operated by the Brazilian Institute for Geography and Statistics. It was possible to monitor the spatial and temporal variations in TEC over Brazil continuously during both day and night with a temporal interval of 10 min and a spatial resolution of about 400 km. The daytime equatorial ionization anomaly (EIA) and post-sunset plasma enhancement (PS-EIA) were monitored over an area corresponding to a longitudinal extension of 4000 km in South America. Considerable day-to-day variation was observed in EIA and PS-EIA. A large latitudinal and longitudinal gradient of TEC indicated a significant ionospheric range error in application of the GNSS positioning system. Large-scale plasma bubbles after sunset were also mapped over a wide range. Depletions with longitudinally separated by more than 800 km were observed. They were extended by more than 2000 km along the magnetic field lines and drifted eastward. It is expected that 2-dimensional TEC mapping can serve as a useful tool for diagnosing ionospheric weather, such as temporal and spatial variation in the equatorial plasma trough and crest, and particularly for monitoring the dynamics of plasma bubbles.     

基于树形马氏链模型的可靠性分析方法

复杂系统的极限状态函数非线性程度较高，在进行可靠性分析时，易导致失效概率的计算误差大、效率低，针对上述问题，提出了树形马氏链（TMC）算法和基于该算法的可靠性分析方法。树形马氏链是对原始马尔可夫链的改进，其状态转移过程更加灵活，具备局部多链并行和自适应探索失效域边界的特性。树形马氏链通过多候选状态点扩大对失效域信息的收集，生成能充分反映失效分布特征的样本，对该样本进行自适应核密度估计得到近似最优的重要抽样分布密度函数，从而提高计算结果的准确度。文末的数值算例和工程算例验证了算法性能，计算结果表明算法对设计点、抽样起点的位置不敏感，处理强非线性及复杂串联系统问题时，能在少样本量下得到相对高准确度的计算结果，且在样本量改变时，计算结果相对稳定可靠；工程算例给出了所提方法在实际问题下的效率，体现了所提方法的工程应用价值。     

Physical–chemical properties of plasma membrane and function of erythrocytes of cosmonauts after long-term space flight

We studied microfluidity and selective ion permeability of plasma membranes and O₂-binding properties of erythrocytes of cosmonauts during early rehabilitation after a long-term space flight (LTSF). Microfluidity of plasma membranes in surface regions was found to undergo a reversible decrease during 13–15 days following LTSF, which was accompanied by a reversible increase in relative cholesterol content. Cosmonauts’ erythrocytes revealed an increased activity of Na/H-exchanger and K_Ca-channel as well as a decrease in number of discocytes and increase in number of echinocytes, stomatocytes and knizocytes. Total hemoglobin content as well as oxyhemoglobin content were lowered after the LTSF, while the affinity of hemoglobin to O₂ was advanced. It is suggested that the changes in Hb properties, microfluidity and selective permeability of plasma membranes following the elevated cholesterol content in the membranes can decrease tissue supply with O₂.     

A study of long-term climatology of ionospheric irregularities by using GPS phase fluctuations at the Brazilian longitudes

We have examined the ionospheric plasma irregularities that were recorded by using three ground-based receivers of the global positioning system (GPS) located at Brazilian longitudes during the period of a complete solar cycle, 1995–2005. The statistic results show that ionospheric irregularities are very easy to occur in December solstice months but rare to occur in June solstice months. Besides, the occurrence rates of irregularities in both December and June solstice months are little dependent on solar activity. However, in equinoctial months, the development of irregularities is obviously dependent on solar activity. There is a new finding in this study that if strong irregularities are distinguished from moderate ones, their occurrence rates would increase with solar activity during the December solstice months.     

On the motions of RHESSI flare footpoints

The footpoint motions of flare hard X-ray (HXR) sources are directly related to the reconnection scenario of a solar flare. In this work, we tried to extract the information of footpoint motions for a number of flares observed with RHESSI. We found that the RHESSI flare results of the footpoint motions strongly support the classification proposed from the observations of YOHKOH/HXT. Furthermore, it is found that a flare can consist of two types of footpoint motions. We discussed the connections of the footpoint motions with the two-dimensional reconnection models.     

Nighttime ionosphere–thermosphere coupling observed during an intense geomagnetic storm

The electrodynamics of the ionosphere in the tropical region presents various scientific aspects, which remain subject of intensive investigations and debates by the scientific community. During the year 2002, in a joint project between the Universidade do Vale do Paraíba (UNIVAP) and Universidade Luterana do Brasil (ULBRA), a chain of three Canadian Advanced Digital Ionosondes (CADIs) was established nearly along the geomagnetic meridian direction, for tropical ionospheric studies, such as, changes and response due to geomagnetic disturbances and thermosphere–ionosphere coupling and the generation and dynamics of ionospheric irregularities, in the Brazilian sector. The locations of the three ionosondes stations are São José dos Campos (23.2°S, 45.9°W, dip latitude 17.6°S – under the southern crest of equatorial ionospheric anomaly), Palmas (10.2°S, 48.2°W, dip latitude 5.5°S – near the magnetic equator) and Manaus (2.9°S, 60.0°W, dip latitude 6.4°N – between the geographic and geomagnetic dip equators). It should be pointed out that Palmas and Manaus are located on the opposite sides of the magnetic equator but both are south of the geographic equator. The three CADIs work in time-synchronized mode and obtain ionograms every 5 min. This configuration of the ionospheric sounding stations allowed us to study the F-region dynamics during geomagnetically disturbed period in the meridional direction. Just after the installation and testing of the three CADIs, on September 05, 2002 a coronal mass ejection (CME) left the Sun and about 2 days after the CME left the Sun, it reached the Earth’s magnetosphere and complex and multi step events took place during the period September 07–09. In the study we note that the equatorial stations located north (Manaus, dip latitude 6.4°N) and south (Palmas, dip latitude 5.5°S) of the dip equator presented significant F-layer height asymmetries during the storm main phase. In addition, the low-latitude station SJC (dip latitude 17.6°S) presented decrease in the F-layer densities (negative phase), whereas Palmas presented increase in the F-layer densities (positive phase) during the main phase. This was followed by positive phase at both the stations. During the first night of the recovery phase a strong formation and evolution of large-scale ionospheric irregularities (equatorial spread-F (ESF)) was observed, but on the second night of the recovery phase, there was strong and almost simultaneous sporadic E (Es) formation at all three stations. During the presence of Es, spread-F formation is not observed, indicating the suppression of spread-F, possibly by sporadic E.