Induced abnormality in Mir- and Earth grown Super Dwarf wheat.

Super-dwarf wheat grown on the Mir space station using the Svet "Greenhouse" exhibited morphological, metabolic and reproductive abnormalities compared with Earth-grown wheat. Of prominent importance were the abnormalities associated with reproductive ontogeny and the total absence of seed formation on Mir. Changes in the apical meristem associated with transition from the vegetative phase to floral initiation and development of the reproductive spike were all typical of 'Super-Dwarf' wheat up to the point of anthesis. Observation of ruptured anthers from the Mir-grown plants revealed what appeared to be normally developed pollen. These pollen gains, however, contained only one nuclei, while normal viable pollen is tri-nucleate. A potentially important difference in the flight experiment, compared with ground reference studies, was the presence of a high level of atmospheric ethylene (1,200 ppb). Ground studies conducted by exposing 'Super-Dwarf' wheat to ethylene just prior to anthesis resulted in manifestation of the same abnormalities observed in the space flight samples.     

Induction and repair of DNA strand breaks in bovine lens epithelial cells after high LET irradiation.

The lens epithelium is the initiation site for the development of radiation induced cataracts. Radiation in the cortex and nucleus interacts with proteins, while in the epithelium, experimental results reveal mutagenic and cytotoxic effects. It is suggested that incorrectly repaired DNA damage may be lethal in terms of cellular reproduction and also may initiate the development of mutations or transformations in surviving cells. The occurrence of such genetically modified cells may lead to lens opacification. For a quantitative risk estimation for astronauts and space travelers it is necessary to know the relative biological effectiveness (RBE), because the spacial and temporal distribution of initial physical damage induced by cosmic radiation differ significantly from that of X-rays. RBEs for the induction of DNA strand breaks and the efficiency of repair of these breaks were measured in cultured diploid bovine lens epithelial cells exposed to different LET irradiation to either 300 kV X-rays or to heavy ions at the UNILAC accelerator at GSI. Accelerated ions from Z=8 (O) to Z=92 (U) were used. Strand breaks were measured by hydroxyapatite chromatography of alkaline unwound DNA (overall strand breaks). Results showed that DNA damage occurs as a function of dose, of kinetic energy and of LET. For particles having the same LET the severity of the DNA damage increases with dose. For a given particle dose, as the LET rises, the numbers of DNA strand breaks increase to a maximum and then reach a plateau or decrease. Repair kinetics depend on the fluence (irradiation dose). At any LET value, repair is much slower after heavy ion exposure than after X-irradiation. For ions with an LET of less than 10,000 keV micrometers-1 more than 90 percent of the strand breaks induced are repaired within 24 hours. At higher particle fluences, especially for low energetic particles with a very high local density of energy deposition within the particle track, a higher proportion of non-rejoined breaks is found, even after prolonged periods of incubation. At the highest LET value (16,300 keV micrometers-1) no significant repair is observed. These LET-dependencies are consistent with the current mechanistic model for radiation induced cataractogenesis which postulates that genomic damage to the surviving fraction of epithelial cells is responsible for lens opacification.     

First estimations of the aerosol optical thickness using Langley Method at Southern Brazil (29.4°S, 53.8°W)

The first estimations of the aerosol optical thickness (AOT) using Langley Method at Southern Space Observatory (SSO) at Southern Brazil (29.4°S, 53.8°W) are presented. In addition to ozone and sulphur dioxide columns, AOT can be obtained using Brewer Spectrophotometer at specific wavelengths: 306.3, 310.1, 313.5, 316.8 and 320.1 nm. The AOT was obtained for the period from November/2002 to May/2003. Very low AOT averages were obtained, whose values were about 0.21 ± 0.03 at 306.3 nm, 0.21 ± 0.02 at 310.1 nm, 0.19 ± 0.02 at 313.5 nm, 0.20 ± 0.02 at 316.8 nm and 0.20 ± 0.02 at 320.0 nm for all period analysed. Different behaviour of AOT were obtained at two daily specific periods of aerosol accumulation, one in the afternoons from November/2002 to February/2003, caused mainly by a mild biomass burning season’s in the region and other in the mornings from March to May/2003, due the high relative humidity presented in the region studied.     

Magnetar descendants in HMXBs?

We investigate the relation between the pulse and orbital periods in high-mass X-ray binaries (HMXBs), in order to find the candidates of magnetar descendants in HMXBs. We suggest that magnetar descendants can be found among the HMXBs with relatively shorter orbital periods and longer pulse periods.     

Spectra and time variability of black-hole binaries in the low/hard state

We propose a jet model for the low/hard state of galactic black-hole X-ray sources which explains the energy spectra from radio to X-rays and a number of timing properties in the X-ray domain such as the time lag spectra, the hardening of the power density spectra and the narrowing of the autocorrelation function with increasing photon energy. The model assumes that (i) there is a magnetic field along the axis of the jet, (ii) the electron density in the jet drops inversely proportional to distance, (iii) the jet is “hotter” near its center than at its periphery, and (iv) the electrons in the jet follow a power-law distribution function. We have performed Monte Carlo simulations of Compton upscattering of soft photons from the accretion disk and have found power-law high-energy spectra with photon-number index in the range 1.5–2 and cutoff at a few hundred keV, power-law time lags versus Fourier frequency with index 0.8, and an increase of the rms amplitude of variability and a narrowing of the autocorrelation function with increasing photon energy as they have been observed in Cygnus X-1. The spectrum at long wavelengths (radio, infrared, optical) is modeled to come from synchrotron radiation of the energetic electrons in the jet. We find flat to inverted radio spectra that extend from the radio up to about the optical band. For magnetic field strengths of the order 10⁵–10⁶ G at the base of the jet, the calculated spectra agree well in slope and flux with the observations.     

Multiband echo tomography of Sco X-1

We present preliminary results of a simultaneous X-ray/optical campaign of the prototypical LMXB Sco X-1 at 1–10 Hz time resolution. Lightcurves of the high excitation Bowen/HeII emission lines and a red continuum at λ_c  6000 Å were obtained through narrow interference filters with ULTRACAM, and these were cross-correlated with simultaneous RXTE X-ray lightcurves. We find evidence for correlated variability, in particular when Sco X-1 enters the Flaring branch. The Bowen/HeII lightcurves lag the X-ray lightcurves with a light travel time which is consistent with reprocessing in the companion star while the continuum lightcurves have shorter delays consistent with reprocessing in the accretion disc.     

Changes in surface UV solar irradiance and ozone over the balkans during the eclipse of August 11, 1999

Intensive measurements of UV solar irradiance, total ozone and surface ozone were carried out during the solar eclipse of 11 August 1999 at Thessaloniki, Greece and Stara Zagora, Bulgaria, located very close to the footprint of the moon's shadow during the solar eclipse with the maximum coverage of the solar disk reaching about 90% and 96% respectively. It is shown that during the eclipse the diffuse component is reduced less compared to the decline of the direct solar irradiance at the shorter wavelengths. A 20-minute oscillation of erythemal UV-B solar irradiance was observed before and after the time of the eclipse maximum under clear skies, indicating a possible 20-minute fluctuation in total ozone presumably caused by the eclipse induced gravity waves. The surface ozone measurements at Thessaloniki display a decrease of around 10–15 ppbv during the solar eclipse. Similarly, ozone profile measurements with a lidar system indicate a decrease of ozone up to 2 km during the solar eclipse. The eclipse offered the opportunity to test our understanding of tropospheric ozone chemistry. The use of a chemical box model suggested that photochemistry can account for a significant portion of the observed surface ozone decrease.     

Life sciences flight hardware development for the International Space Station.

During the construction phase of the International Space Station (ISS), early flight opportunities have been identified (including designated Utilization Flights, UF) on which early science experiments may be performed. The focus of NASA's and other agencies' biological studies on the early flight opportunities is cell and molecular biology; with UF-1 scheduled to fly in fall 2001, followed by flights 8A and UF-3. Specific hardware is being developed to verify design concepts, e.g., the Avian Development Facility for incubation of small eggs and the Biomass Production System for plant cultivation. Other hardware concepts will utilize those early research opportunities onboard the ISS, e.g., an Incubator for sample cultivation, the European Modular Cultivation System for research with small plant systems, an Insect Habitat for support of insect species. Following the first Utilization Flights, additional equipment will be transported to the ISS to expand research opportunities and capabilities, e.g., a Cell Culture Unit, the Advanced Animal Habitat for rodents, an Aquatic Facility to support small fish and aquatic specimens, a Plant Research Unit for plant cultivation, and a specialized Egg Incubator for developmental biology studies. Host systems (Figure 1A, B: see text), e.g., a 2.5 m Centrifuge Rotor (g-levels from 0.01-g to 2-g) for direct comparisons between g and selectable g levels, the Life Sciences Glovebox for contained manipulations, and Habitat Holding Racks (Figure 1B: see text) will provide electrical power, communication links, and cooling to the habitats. Habitats will provide food, water, light, air and waste management as well as humidity and temperature control for a variety of research organisms. Operators on Earth and the crew on the ISS will be able to send commands to the laboratory equipment to monitor and control the environmental and experimental parameters inside specific habitats. Common laboratory equipment such as microscopes, cryo freezers, radiation dosimeters, and mass measurement devices are also currently in design stages by NASA and the ISS international partners.     

He-like Ar xvii triplet observed by RESIK

We present the observations of He-like Ar triplet lines obtained by RESIK spectrometer aboard CORONAS-F. Interpretation of intensity ratios between triplet lines of lower Z elements is known to provide useful diagnostics of plasma conditions within the emitting source. Here, we investigate whether triplet line ratios are useful for interpretation of higher Z element spectra. A high sensitivity, low background and precise absolute calibration of RESIK allow to consider in addition also the continuum contribution. This provides a way to determine the Ar absolute abundance from the observed triplet component ratios. The method is presented and the results are shown for two selected flares. Derived values of Ar absolute abundance for these flares are found to be similar: 2.6 × 10⁻⁶ and 2.9 × 10⁻⁶. They fall in the range between presently accepted Ar photospheric and coronal abundances.