Evaluation of bleomycin-induced chromosome aberrations under simulated microgravity conditions in human lymphocytes using "FISH" techniques

In the present investigation we report the effects of simulated microgravity conditions (clinostat) on the induction of chromosomal aberrations in human lymphocytes in vitro by (R) Bleomycin. Chromosomal aberrations have been analysed by means of fluorescent in situ hybridisation (FISH) and chromosome-specific composite DNA probes (chromosome painting). The results obtained show that, under simulated microgravity conditions, the levels of both symmetrical and asymmetrical (dicentrics, rings), the number of cells bearing "complex" aberrations and hence the total numbers of aberrations were significantly elevated at any of the dose-levels assayed, compared to the parallel treatments performed as 1g control ("ground"). Furthermore, the ratio symmetrical:asymmetrical translocations was markedly elevated under simulated microgravity conditions, compared to the findings usually observed under "normal" 1g conditions. On these bases, we are much inclined to believe that simulated microgravity, rather than limiting the resealing of DNA double strand breaks (DSB's) induced by genotoxic agents is influencing in terms of enhancement the misrejoining of DSB's which is actually responsible for the fixation of the original lesions to DNA into chromosomal aberrations. In addition, the possible different misrepair processes leading to the formation of symmetrical and asymmetrical translocations might be differentially influenced by microgravity being the symmetrical translocations significantly more represented.     

The action of simulated and true weightlessness on the digestive tract of rats

Rats on board the Soviet Cosmos 936 satellite for 18$\text{1}\text{2}$ days showed a decreased glycoprotein secretion from the salivary mucous glands, stomach and intestine, and an increased leucine aminopeptidase and acid phosphatase content from the small intestine. Grimelius positive cells were activated. One group of rats were centrifuged at 1 g during the flight to simulate terrestrial gravity. Some investigations have suggested that under these conditions muscular and cardiac disorders diminished. In the digestive tract the benefits of centrifugation at 1 g are minimal and limited to a few glycoprotein components. The digestive changes are probably the expression of a stress response, unrelated to weightlessness. Similar changes, concomitant with a glycocorticoid hypersecretion, were found in rats after 15 days of hypokinesia on Earth. These digestive changes persisted even in adrenalectomized rats.     

Bayesian approach to retrieval of vertical ozone profile from radiometry data

A technique for retrieving vertical distributions (profiles) of atmospheric gas constituents from data of passive remote sensing of the atmosphere is proposed. The goal of the technique based on the statistical (Bayesian) approach to solution of inverse problems is construction of probability distribution for a sought quantity throughout the interval of the studied heights. It is assumed that initial data contain measurement noise, and a priori information about properties of the profile is used. It is proposed to approximate the sought profile by a function in the form of an artificial neural network. This approximation allows optimal inclusion of a priori information into retrieval procedure, thus ensuring the most effective regularization of the problem. Efficiency of the proposed technique is demonstrated on an example of retrieval of vertical ozone profile from data of ground-based sounding of the atmosphere in the millimeter wavelength range. Results of profile retrieval from model data and from spectra of radiation temperature of the atmosphere measured in the Apatity (67° N, 33° E) in the winter of 2002–2003 are presented.     

Ontogeny of plants under various gravity condition.

The results of experiments performed under conditions of microgravity (MG) or under its simulation on the horizontal clinostat (HC) with the callus, seedlings of various species and embryogenic structures have revealed a definite role of gravity as an ecological factor in the processes of cytomorphogenesis, growth, and development. The transformation of differentiated somatic cells of arabidopsis seed into undifferentiated callus was not inhibited under MG, though modifications of the whole callus morphology and of mean cell and nucleus size were observed. The morphogenesis of polar structures such as root-hair bearing cells of Lactuca primary root has been shown to be modified in the course of differentiation under mass acceleration diminished below 0.1 g. Seed germination and seedling morphogenesis under MG follow their normal course, but a significant stimulation of shoot growth with no effect on primary root growth has been determined. A successful in vitro regeneration of Nicotiana tabacum plantlets from leaf cells and subsequent formation of shoots and roots on a continuously rotating HC as well as the formation of viable seeds during seed-to-seed growth of Arabidopsis plants under MG have indicated that gravity plays but a limited role in the processes of embryogenesis and organogenesis.     

Chromospheric line emission in seismically active flares

Some flares are known to drive seismic transients into the solar interior. The effects of these seismic transients are seen in helioseismic observations of the Sun’s surface thousands of km from their sources in the hour succeeding the impulsive phase of the flare. Energetic particles impinging from the corona into the chromosphere are known to drive strong, downward-propagating shocks in active region chromospheres during the impulsive phases of flares. H observations have served as an important diagnostic of these shocks, showing intense emission with characteristic transient redshifts. In most flares no detectable transients penetrate beneath the active region photosphere. In those that do, there is a strong correlation between compact white-light emission and the signature of seismic emission. This study introduces the first known H observations of acoustically active flares, centered in the core of the line. The morphology of line-core emission H in the impulsive phase of the flare is similar to that of co-spatial line-core emission in NaD₁, encompassing the site of seismic emission but more extended. The latter shows a compact red shift in the region of seismic emission, but a similar feature is known to appear in a conjugate magnetic footpoint from which no seismic emission emanates. Radiative MHD modelling based on the profiles of chromospheric line emission during the impulsive phases of flares can contribute significantly to our understanding of the mechanics of flare acoustic emission penetrating into the solar interior and the conditions under which it occurs.     

Nonconscious intelligence in the universe

Animals lacking humanoid intelligence have evolved systems indistinguishable in function, if not in structure, from systems built by humans. Although radio communication has never been verified in animals, it is completely feasible biologically. If such systems are present in non-intelligent organisms on other planets, then our chances of detecting life in the universe by current SETI methods are greatly enhanced.     

Polarization Studies of Contribution of Aerosol Scattering to the Glow of Twilight Sky

The paper is devoted to studies and a quantitative estimation of the influence of scattering of direct solar radiation by particles of tropospheric aerosols in the period of light twilight, close to sunrise and sunset. Polarization measurements of the twilight sky in two broad spectral bands lying near the standard bands V and R form the basis of these studies. The measurements were carried out in 2002 and 2003 at the Southern Laboratory (Crimea) of the Sternberg Astronomical Institute. The twilight period during which the single aerosol scattering becomes significant is isolated. The character of its influence on the intensity and polarization of the twilight sky is demonstrated. A method is suggested to evaluate polarization of the single aerosol scattering and its contribution to the general brightness of the twilight sky background. The method is based on differential characteristics of the background near the zenith and on their correlations with each other.     

Occurrence of brain tumors in rhesus monkeys exposed to 55-MeV protons.

Twenty-year observation of monkeys exposed to single doses of high energy protons simulating solar particles revealed that the most prevalent fatal cancers were brain tumors in the group of animals exposed to 55-MeV protons. Of 72 animals (50 males and 22 females) receiving 0.25 to 8.0 Gy total body surface dose, nine developed fatal tumors classified as grade IV astrocytoma or glioblastoma multiforme. The latent period for tumor development ranged from 14 months to 20 years, with a median of 5 years. Doses associated with the tumors were 4.0 to 8.0 Gy. Eight males and one female were affected. Depth-dose determinations suggest that the high incidence of cerebral neoplasia is associated with the Bragg Peak energy distribution of the 55-MeV protons. Comparison of the tumor incidence with that in humans with brain exposures incidental to radiotherapy indicates a high biological effectiveness compared with gamma radiation. Studies are in progress to attempt to replicate the results in rodents and establish a dose-response curve for proton-induced brain tumors.     

Atmospheric Ion-induced Aerosol Nucleation

Ion-induced nucleation has been suggested to be a potentially important mechanism for atmospheric aerosol formation. Ions are formed in the background atmosphere by galactic cosmic rays. A possible connection between galactic cosmic rays and cloudiness has been However, the predictions of current atmospheric nucleation models are highly uncertain because the models are usually based on the liquid drop model that estimates cluster thermodynamics based on bulk properties (e.g., liquid drop density and surface tension). Sulfuric acid (H₂SO₄) and water are assumed to be the most important nucleating agents in the free troposphere. Measurements of the molecular thermodynamics for the growth and evaporation of cluster ions containing H₂SO₄ and H₂O were performed using a temperature-controlled laminar flow reactor coupled to a linear quadrupole mass spectrometer as well as a temperature-controlled ion trap mass spectrometer. The measurements were complemented by quantum chemical calculations of the cluster ion structures. The analysis yielded a complete set of H₂SO₄ and H₂O binding thermodynamics extending from molecular cluster ions to the bulk, based on experimental thermodynamics for the small clusters. The data were incorporated into a kinetic aerosol model to yield quantitative predictions of the rate of ion-induced nucleation for atmospheric conditions. The model predicts that the negative ion-H₂SO₄-H₂O nucleation mechanism is an efficient source of new particles in the middle and upper troposphere.     

Microphysics of Quasi-parallel Shocks in Collisionless Plasmas

Shocks in collisionless plasmas require dissipation mechanisms which couple fields and particles at scales much less than the conventional collisional mean free path. For quasi-parallel geometries, where the upstream magnetic field makes a small angle to the shock normal direction, wave-particle coupling produces a broad transition zone with large amplitude, nonlinear magnetic pulsations playing an important role. At high Mach numbers, ion reflection and acceleration are dominant processes which control the structure and dissipation at the shock. Accelerated particles produce a precursor, or foreshock, characterized by low frequency magnetic waves which are convected by the plasma flow into the shock transition zone. The interplay between energetic particles, waves, ion reflection and acceleration leads to a complicated interdependent system. This review discusses the spacecraft observations which have motivated the current view of the high Mach number quasi-parallel shock, and the theories and simulation studies which have led to a better understanding of the microphysics on which the quasi-parallel shock depends.