Microbially induced iron precipitation associated with a neutrophilic spring at Borra Caves, Vishakhapatnam, India

The present investigation uncovers various pieces of evidence for the possible biologically induced mineralization in iron mats associated with a pH-neutral spring in the Borra caves, Vishakhapatnam, India. Electron microscopy [scanning electron microscopy (SEM) and transmission electron microscopy (TEM)] demonstrated large numbers of (i) hollow tubes (diameter ～1?μm) resembling sheaths of the iron-oxidizing bacteria Leptothrix, (ii) thin (diameter <1?μm) solid fibers of uncertain origin, (iii) nanoscale subspherical to irregularly shaped particles encrusting tubes and fibers, and (iv) aggregates of broken and partially disintegrated sheaths, fibers, and particles embedded in extracellular polymeric substances (EPS) occasionally including microbial cells. X-ray microanalyses by energy dispersive spectroscopy (EDS) revealed that the mat accumulated largely Fe but also smaller amounts of Si and traces of P and Ca. Particles rich in Si and Al (possibly kaolinite) and Ca (carbonate) were also observed. High-resolution TEM/EDS of unstained ultrathin sections suggests that microbial sheaths were highly mineralized by amorphous to cryptocrystalline Fe-rich phases and less frequently by other fine-grained and fibrous authigenic claylike minerals. Total number of microorganisms in the iron mats was 5.8×10(5) cells, g sed(-1) (wet weight). Analysis of the 16S rRNA gene diversity revealed microorganisms assigned to eight different phyla [Proteobacteria (62%), Chloroflexi (8%), Bacteroidetes (7%), Planctomycetes (1%), Actinobacteria (5%), Acidobacteria (6%), Nitrospira (1%), Firmicutes (5%)]. Within the Proteobacteria, Betaproteobacteria was the predominant class, which accounted for 28% of the sequences. Within this class some obvious similarities between the obtained sequences and sequences from other cave systems could be seen, especially sequences affiliated with Leptothrix, Siderooxidans, Crenothrix, Comamonadaceae, Dechloromonas, and many uncultured Betaproteobacteria. Four (4%) of the sequences could not be assigned to phylum level but were affiliating with the candidate division TM7 (2%), candidate division OP11 (1%), and candidate division WWE3 (1%). The results allow us to infer a possible relationship of microbial sheaths, EPS, and the iron precipitates to microbial community diversity in the Borra cave springs. Understanding biogenic iron oxides in caves has important astrobiological applications as it provides a potential tool for the detection of extraterrestrial life.     

LIFE: Life Investigation For Enceladus A Sample Return Mission Concept in Search for Evidence of Life

Abstract Life Investigation For Enceladus (LIFE) presents a low-cost sample return mission to Enceladus, a body with high astrobiological potential. There is ample evidence that liquid water exists under ice coverage in the form of active geysers in the "tiger stripes" area of the southern Enceladus hemisphere. This active plume consists of gas and ice particles and enables the sampling of fresh materials from the interior that may originate from a liquid water source. The particles consist mostly of water ice and are 1-10?μ in diameter. The plume composition shows H(2)O, CO(2), CH(4), NH(3), Ar, and evidence that more complex organic species might be present. Since life on Earth exists whenever liquid water, organics, and energy coexist, understanding the chemical components of the emanating ice particles could indicate whether life is potentially present on Enceladus. The icy worlds of the outer planets are testing grounds for some of the theories for the origin of life on Earth. The LIFE mission concept is envisioned in two parts: first, to orbit Saturn (in order to achieve lower sampling speeds, approaching 2 km/s, and thus enable a softer sample collection impact than Stardust, and to make possible multiple flybys of Enceladus); second, to sample Enceladus' plume, the E ring of Saturn, and the Titan upper atmosphere. With new findings from these samples, NASA could provide detailed chemical and isotopic and, potentially, biological compositional context of the plume. Since the duration of the Enceladus plume is unpredictable, it is imperative that these samples are captured at the earliest flight opportunity. If LIFE is launched before 2019, it could take advantage of a Jupiter gravity assist, which would thus reduce mission lifetimes and launch vehicle costs. The LIFE concept offers science returns comparable to those of a Flagship mission but at the measurably lower sample return costs of a Discovery-class mission. Key Words: Astrobiology-Habitability-Enceladus-Biosignatures. Astrobiology 12, 730-742.     

A new spectral index to detect Poaceae grass abundance in Mongolian grasslands

The objectives of the present study were to develop a new index based on remotely-sensed data for detecting the abundance of grasses in the family Poaceae, which has a high palatability for livestock in Mongolia, and to map the distribution of these grasses in the semi-arid Mongolian steppes. We measured ground-based spectral reflectance of pure plant leaves – including Poaceae grasses – and soils, as well as in-situ in the Mongolian grasslands. The hyper-spectral data, taken by a spectroradiometer, were converted into four multi-spectral bands (i.e., blue, green, red, and NIR) to simulate satellite-based imagery data. In order to magnify the characteristics of the spectral signal of Poaceae, NGBDI (Normalized Green-Blue Difference Index), NGRDI (Normalized Green-Red Difference Index), NDVI (Normalized Difference Vegetation Index), NNBDI (Normalized NIR-Blue Difference Index) were calculated from the four multi-spectral reflectance values. Poaceae Abundance Index (PAI) was derived by combining these four normalized difference indices. PAI was found out to be a good indicator to discriminate Poaceae grass from the other plant spectral data.     

Removing orbital debris with lasers

Orbital debris in low Earth orbit (LEO) are now sufficiently dense that the use of LEO space is threatened by runaway collision cascading. A problem predicted more than thirty years ago, the threat from debris larger than about 1 cm demands serious attention. A promising proposed solution uses a high power pulsed laser system on the Earth to make plasma jets on the objects, slowing them slightly, and causing them to re-enter and burn up in the atmosphere. In this paper, we reassess this approach in light of recent advances in low-cost, light-weight modular design for large mirrors, calculations of laser-induced orbit changes and in design of repetitive, multi-kilojoules lasers, that build on inertial fusion research. These advances now suggest that laser orbital debris removal (LODR) is the most cost-effective way to mitigate the debris problem. No other solutions have been proposed that address the whole problem of large and small debris. A LODR system will have multiple uses beyond debris removal. International cooperation will be essential for building and operating such a system.     

Realization of modes of satellite attitude motion with a small level of microaccelerations using electromechanical executive devices

Quasi-static microaccelerations are estimated for a satellite specially designed to perform space experiments in the field of microgravity. Three modes of attitude motion of the spacecraft are considered: passive gravitational orientation, orbital orientation, and semi-passive gravitational orientation. In these modes the lengthwise axis of the satellite is directed along the local vertical, while solar arrays lie in the orbit plane. The second and third modes are maintained using electromechanical executive devices: flywheel engines or gyrodynes. Estimations of residual microaccelerations are performed with the help of mathematical modeling of satellite’s attitude motion under the action of gravitational and aerodynamic moments, as well as the moment produced by the gyro system. It is demonstrated that all modes ensure rather low level of quasi-static microaccelerations on the satellite and provide for a fairly narrow region of variation for the vector of residual microacceleration. The semi-passive gravitational orientation ensures also a limited proper angular momentum of the gyro system.     

Activation of the tail open part during the magnetospheric storm

In each polar cap (PC) we mark out “old PC” observed during quiet time before the event under consideration, and “new PC” that emerges during the substorm framing the old one and expanding the PC total area. Old and new PCs are the areas for the magnetosphere old and new tail lobes, respectively. The new lobe variable magnetic flux Ψ₁ is usually assumed to be active, i.e. it provides the electromagnetic energy flux (Poynting flux) ɛ′ transport from solar wind (SW) into the magnetosphere. The old lobe magnetic flux Ψ₂ is supposed to be passive, i.e. it remains constant during the disturbance and does not participate in the transporting process which would mean the old PC electric field absolute screening from the convection electric field created by the magnetopause reconnection. In fact, screening is observed, but far from absolute. We suggest a model of screening and determine its quantitative characteristics in the selected superstorm. The coefficient of a screening is the β = Ψ₂/Ψ₀₂, where Ψ₀₂ = const is open magnetic flux through the old PC measured prior to the substorm, and Ψ₂ is variable magnetic flux through the same area measured during the substorm. We consider three various regimes of disturbance. In each, the coefficient β decreased during the loading phase and increased at the unloading phase, but the rates and amplitudes of variations exhibited a strong dependence on the regime. We interpreted decrease in β as a result of involving the old PC magnetic flux Ψ₂, which was considered to be constant earlier, in the Poynting flux ɛ′ transport process from solar wind into the magnetosphere. Transport process weakening at the subsequent unloading phase creates increase in β. Estimates showed that coefficient β during each regime and the computed Poynting flux ɛ′ varied manifolds. In general, unlike the existing substorm conception, the new scenario describes an unknown earlier of tail lobe activation process during a substorm growth phase that effectively increases the accumulated tail energy for the expansion and recovery phases.     

Relationship between the fluxes of relativistic electrons at geosynchronous orbit and the level of ULF activity on the Earth’s surface and in the solar wind during the 23rd solar activity cycle

We present the results of a cross-correlation analysis made on the basis of Spearman’s rank correlation method. The quantities to correlate are daily values of the fluence of energetic electrons at a geosynchronous orbit, intensities of ground and interplanetary ultra-low-frequency (ULF) oscillations in the Pc5 range, and parameters of the solar wind. The period under analysis is the 23rd cycle of solar activity, 1996–2006. Daily (from 6 h to 18 h of LT) magnetic data at two diametrically opposite observatories of the Intermagnet network are taken as ground-based measurements. The fluxes of electrons with energies higher than 2 MeV were measured by the geosynchronous GOES satellites. The data of magnetometers and plasma instruments installed on ACE and WIND spacecraft were used for analysis of the solar wind parameters and of the oscillations of the interplanetary magnetic field (IMF). Some results elucidating the role played by interplanetary ULF waves in the processes of generation of magneospheric oscillations and acceleration of energetic electrons are obtained. Among them are (i) high and stable correlation of ground ULF oscillations with waves in the solar wind; (ii) closer link of mean daily amplitudes of both interplanetary and ground oscillations with ‘tomorrow’ values of the solar wind velocity than with current values; and (iii) correlation of the intensity of ULF waves in the solar wind, normalized to the IMF magnitude, with fluxes of relativistic electrons in the magnetosphere.     

SSA: Where does Europe stand now?

With the growing need for monitoring and awareness of all the objects in space (including debris) now recognized, the types of effort required towards this end are discussed, with the emphasis on European activities. A number of European documents acknowledging the problem are cited and initiatives to promote space situational awareness (SSA) are examined. While there is currently no provision in the EU budget beyond 2013 for protecting ground and space assets from space environmental threats, an encouraging degree of bilateral cooperation between member states suggests that an SSA capability is emerging in Europe.     

Integral method for calculating a turbofan engine thrust reverser jet interacting with an external stream

An integral method is proposed for calculating a jet propagating from the turbofan engine thrust reverser and interacting with a stream formed as a result of the after-landing aircraft run. The calculation results for the PS-90 engine are presented. The calculation data obtained show that the mathematical model developed adequately describes a qualitative pattern of main parameter variation in the sector jet propagating in a stream.     

Investigation of gas dynamics numerical schemes based on the solution of one-dimensional test problems

The application of the optimized dispersion relation preserving scheme (DRP-scheme) in combination with the explicit optimized two-layer Runge-Kutta scheme is presented to solve a system of one-dimensional and quasi-one-dimensional Euler equations using as an example the solution of four test problems, namely, discontinuity disintegration in a tube (Sod’s problem); transfer of the lowamplitude Gaussian pulse; acoustic wave propagation through the transonic nozzle; acoustic wave-shock interaction. Also given are the comparison of the calculation results using different schemes: DRP, CABARET, CE-SE and the standard Lax-Wendroff schemes as well as the solutions obtained with the use of software packages.