Subcellular components of the amphibian egg: insights provided by gravitational studies.

Most cytoplasmic regions of fertilized amphibian eggs move with respect to the gravity vector in experimentally gravity oriented eggs. The pattern and extent of this movement varies among different batches of eggs. This variation in apparent cytoplasmic viscosity (or, conversely, cytoplasmic mobility) can be correlated with variations in subsequent morphogenesis of experimental, gravitationally manipulated eggs. Therefore, the proper interpretation of gravity experiments with amphibian eggs requires that one understand the subcellular basis for this variation on cytoplasmic mobility. Variation in the packing of the major cytoplasmic organelle, the yolk platelets, or the organization and amount of cytoskeletal components may explain the variation in cytoplasmic mobility. Evidence is presented that the variation in yolk volume density (fraction of total cytoplasmic volume occupied by yolk platelets) does not account for the variation in cytoplasmic mobility in Xenopus laevis eggs. Experimental evidence from cold-shocked inverted eggs indicates that microtubules may be involved in determining cytoplasmic mobility. However, quantitative evidence that the microtubule levels and state of the microtubules (polymerized vs. non-polymerized) in the whole Xenopus laevis egg does not correlate directly with cytoplasmic mobility is presented. The apparent conflict these data represent regarding the role of the cytoskeleton in determining cytoplasmic mobility is discussed.     

Plant cell plasma membrane structure and properties under clinostatting.

Structural-functional organization of plasma membrane of pea roots seedling was investigated by methods of chemiluminescence, fluorescence probes, chromatography and freeze-fracture studies under normal conditions and clinostatting. Phase character of lipid peroxidation intensity was fixed. The initial phase of this process is characterized by lipid peroxidation decreasing with its next induction. The primary changes depending on free-radical mechanisms of lipid peroxidation were excellently revealed by chemiluminescence. Plasmalemma microviscosity increased on the average of 15-20% under microgravity at the initial stages of its phenomenon. There were major changes of phosphatidilcholine and phosphatidilethanolamine contents. The total quantity of phospholipids remained rather stable. Changes of phosphatide acid concentration point to degradation and phospholipids biosynthesis. There were increases of unsaturated fatty acids mainly at the expense of linoleic and linolenic acids and also a decrease of saturated fatty acid content at the expense of palmitic and stearic acids. Unsaturation index of fatty acids increased as well. On the whole fatty acid composition was variable in comparison with phospholipids. Probably it is one of mechanisms of maintaining of microviscosity within definite limits. Considerable structural changes in organization of plasmalemma protein-lipid complex were not revealed by the freeze-fracture studies.     

Gravitational biology and the mammalian circadian timing system.

Mammals have evolved under the influence of many selective pressures. Two of these pressures have been the static force of gravity and the daily variations in the environment due to the rotation of the earth. It is now clear that each of these pressures has led to specific adaptations which influence how organisms respond to changes in either gravity or daily time cues. However, several unpredicted responses to altered gravitational environments occur within the homeostatic and circadian control systems. These results may be particularly relevant to biological and medical issues related to spaceflight. This paper demonstrates that the homeostatic regulation of rat body temperature, heart rate, and activity become depressed following exposure to a 2 G hyperdynamic field, and recovers within 5-6 days. In addition, the circadian rhythms of these same variables exhibit a depression of rhythm amplitude; however, recovery required a minimum of 7 days.     

Long-term preservation of microbial ecosystems in permafrost.

It has been established that significant numbers (up to 10 million cells per gram of sample) of living microorganisms of various ecological and morphological groups have been preserved under permafrost conditions, at temperatures ranging from -9 to -13 degrees C and depths of up to 100 m, for thousands and sometimes millions of years. Preserved since the formation of permafrost in sand-clay sediments of the Pliocene-Quaternary period and in paleosols and peats buried among them, these cells art the only living organisms that have survived for a geologically significant period of time. The complexity of the microbial community preserved varies with the age of the permafrost. Eukaryotes are found only in Holocene sediments; while prokaryotes are found to greater ages, i.e., Pliocene and Pleistocene. The diversity of microorganisms decreases with increasing age of sediments, and as a result cocci and corynebacteria are predominant. Enzyme activity (catalase and hydrolytic enzymes) and photosynthetic pigments (chlorophyll and pheophytin have also been detected in permafrost sediments. These results permit us to outline some approaches to the search for traces of life in the permafrost of Martian sediments by borehole core sampling. It is in the deep horizons (and not on the planet surface), isolated by permafrost from the external conditions, that results similar to those obtained on Earth can be expected.     

A question raised from the observation of dynamic cusp formation: When and where does particle acceleration occur?

We present observations of a C9.4 flare on 2002 June 2 in EUV (TRACE) and X-rays (RHESSI). The multiwavelength data reveal: (1) the involvement of a quadrupole magnetic configuration; (2) loop expansion and ribbon motion in the pre-impulsive phase; (3) gradual formation of a new compact loop with a long cusp at the top during the impulsive phase of the flare; (4) appearance of a large, twisted loop above the cusp expanding outward immediately after the hard X-ray peak; and (5) X-ray emission observed only from the new compact loop and the cusp. In particular, the gradual formation of an EUV cusp feature is very clear. The observations also reveal the timing of the cusp formation and particle acceleration: most of the impulsive hard X-rays (>25 keV) were emitted before the cusp was seen. This suggests that fast reconnection occurred during the restructuring of the magnetic configuration, resulting in more efficient particle acceleration, while the reconnection slowed after the cusp was completely formed and the magnetic geometry was stabilized. This observation is consistent with the observations obtained with Yohkoh/Soft X-ray Telescope (SXT) that soft X-ray cusp structures only appear after the major impulsive energy release in solar flares. These observations have important implications for the modeling of magnetic reconnection and particle acceleration.     

Sikkim's Teesta River fog, mist and dust particle monitoring using monostatic LIDAR

Fog, mist, and atmospheric dust particles, having the dimension of one micrometer or less, play an important role in the deterioration of visibility, as well as in causing local warming in the atmosphere. With an attempt to reduce the deterioration, a scientific approach has to be taken to determine their origins. A monostatic LIDAR may be one of the best instruments for such work. The authors are tempted to develop such a LIDAR for fog, mist, and dust particle monitoring over River Teesta at Sikkim. LIDAR is an acronym for Light Detection And Ranging. What can we do with LIDAR? Measure distance, measure speed, measure rotation, measure chemical composition and concentration, and measure cross-sections of the targets. The digital technique is always utilized for its development which results in better security, lower power consumption, higher power efficiency, higher reliability, lower transmitter power, lower multipath effect, higher interference suppression as compared to an analog system. The commercial systems like disdometer, rain radar, mobile robot, etc., utilizing LIDAR principles are operational in different parts of the world. The authors are highly motivated for such LIDAR development and their development effort follows.     

ROSAT observations of the Chamaeleon star forming cloud

Two soft X-ray images of the Chamaeleon I star forming cloud obtained with the ROSAT Position Sensitive Proportional Counter are presented. Seventy reliable, and perhaps 19 additional, X-ray sources are found. Up to Ninety percent of these sources are certainly or probably identified with T Tauri stars formed in the cloud. Twenty to 35 are probably previously unrecognized ‘weak’ T Tauri (WTT) stars. T Tauri X-ray luminosities range from log , or 10² – 10⁴ times solar levels, with mean in the 0.2–2.5 keV band. The X-ray luminosities of well-studied Chamaeleon cloud members are correlated with a complex of four stellar properties: effective temperature, mass, radius and bolometric luminosity. The spatial distribution, H-R diagram locations of the stars indicate WTT and CTT are coeval. The total premain sequence population of the cloud is likely to be > 100 stars, with WTT stars outnumbering ‘classical’ T Tauri (CTT) stars by 2:1.     

Evaluation of experiments involving the study of plant orientation and growth under different gravitational conditions.

The manifestation of gravitropic reaction in plants has been considered from the phylogenetic point of view. A chart has been suggested according to which it is supposed that the first indications of the ability to identify the direction of the gravitational vector were inherent in the most ancient eukaryotes, which gave rise to green, brown, yellow-green, golden and diatomaceous algae as well as fungi. The experiments on the role of gravity in plant ontogenesis are being continued. The sum total of the data obtained in a number of experiments in space shows that under these conditions a structurally modified but normally functioning gravireceptive apparatus is formed. The data confirming the modification, under changed gravity, of the processes of integral and cellullar growth of the axial organs of seedlings as well as of the anatomo-morphological structure and developmental rates of plants during their prolonged growth in space are presented. It is assumed that this fact testifies to the presence of systems interacting with gravity during plant ontogenesis. At the same time the necessity for further experiments in order to differentiate an immediate biological effect of gravity from the ones conditioned by it indirectly due to the changes in the behavior of liquids and gases is pointed out. The methodological aspects of biological experiments in space as the main source of reliable information on the biological role of gravity are discussed.     

Planetary protection policy overview and application to future missions.

The 1967 treaty on the peaceful uses of outer space reflected both concerns associated with the unknown nature of the space environment and the desire of the world scientific community to preserve the pristine nature of celestial objects until such times as they could be studied in an effective manner. Since 1967, NASA has issued policy directives that have adopted the guidelines of COSPAR for protecting the planets from contamination by Earth organisms and for protecting the Earth from the unknown. This paper presents the current status of planetary protection (quarantine) policy within NASA, and a prospectus on how planetary protection and back contamination issues might be addressed in relation to future missions envisioned for development by NASA either independently, or in cooperation with the space agencies of other nations.     

Planetary protection issues for sample return missions.

Sample return missions from a comet nucleus and the Mars surface are currently under study in the US, USSR, and by ESA. Guidance on Planetary Protection (PP) issues is needed by mission scientists and engineers for incorporation into various elements of mission design studies. Although COSPAR has promulgated international policy on PP for various classes of solar system exploration missions, the applicability of this policy to sample return missions, in particular, remains vague. In this paper, we propose a set of implementing procedures to maintain the scientific integrity of these samples. We also propose that these same procedures will automatically assure that COSPAR-derived PP guidelines are achieved. The recommendations discussed here are the first step toward development of official COSPAR implementation requirements for sample return missions.