Force sensitivity of plant gravisensing.

Rotation at 4, 10, 50 and 100 rpm on a horizontal clinostat and in microgravity exerts limited effects on the morphogenesis of lettuce and cress root statocytes and statoliths if compared with the vertical control or 1 g spaceflight reference centrifuge. However, the average distance of statoliths from the distal wall increases. The pattern of plastid location of microgravity-grown and that of clino-rotated samples has been determined at 10, 50, and 100 rpm. Experiments on the centrifuge-clinostat and spaceflight centrifuge (acceleration forces of 0.005 to 1 g) revealed that the average statolith location depends on the amplitude of acropetally or basipetally directed mass acceleration. Decreasing the acropetally directed force from 1 g to 0.4 g dislocates statoliths towards the cell center possibly mediated by the elastic forces of the cytoskeleton. In statocytes formed on the clinostat or in microgravity, the majority of statoliths are located at the center of the cell. To force the statoliths from the center of the statocyte towards one of its poles, a threshold mass acceleration of 0.01 g is required. Statocytes with centrally-located statoliths are considerably more effective in transducing a gravistimulus than those with distally-located plastids. The latent time of the graviresponse is shorter and the response itself is enhanced in roots grown on the clinostat compared to vertically grown samples. The early phases of graviperception are independent of root growth conditions since presentation time and g-threshold are similar for roots grown stationary and those on a clinostat. We propose a sequence of events in gravitropic stimulation that considers not only the lateral displacement of statoliths, as predicted by the starch-statolith hypothesis, but also its longitudinal motion, together with differential gravisensitivity of mechanotransducing structures along the lower-most longitudinal cell wall.     

Deriving the spin rate/orientation from the quiescent spacecraft ABRIXAS using optical observations

With the loss of the battery system of ABRIXAS shortly after launch and consequent absence of telemetry, there was urgent need to determine ABRIXAS' spin rate and orientation in order to assess the possibility of re-establishing telemetry during periods of full-Sun orbits. We, therefore, conducted optical and video observations of ABRIXAS passages with a 1-second time resolution, and later simulated the optical appearance of ABRIXAS for several passages based on a three-dimensional model of the reflectivity properties of the 2.5m × 1.8m × 1.2m size-satellite. Here we present the results of the comparison of our grid of light curve models with the observations and show (i) how the spin rate of ABRIXAS slowed down between June and December 1999 and (ii) what information can be deduced on the temporal change of the orientation of the spin axis. We conclude with discussing the benefit of using ground based optical observation as a cost effective way to develop information about the orientation of a satellite when there is no telemetry.     

Inactivation, mutation induction and repair in Bacillus subtilis spores irradiated with heavy ions.

Studies on the response of bacterial spores to accelerated heavy ions (HZE particles) help in understanding problems of space radiobiology and exobiology. Layers of spores of Bacillus subtilis strains, differing in repair capabilities, were irradiated with accelerated boron, carbon and neon ions of linear energy transfer (LET) values up to 14000 MeV cm2/g. Inactivation as measured by loss of colony forming ability and induction of mutations as measured by reversion to histidine prototrophy and resistance to 150 micrograms/ml sodium azide were tested, as well as the influence of repair processes on these effects. For inactivation, the cross-sectional values sigma plotted as a function of LET follow a saturation curve. The plateau, which is reached around a LET of 2000 MeV cm2/g, occurs at 2.5 x 10(-9) cm2, a value in good agreement with the dimensions of the spore protoplast. Lethal damage produced at LET values < 2000 MeV cm2/g is reparable. Recombination repair is more effective than excision repair. At higher LET values, lethal damage could not be reconstituted by the repair mechanisms studied. In addition, at these high LET values, the frequency of induced mutations was drastically decreased. The data support the assumption of at least two qualitatively different types of lesion, depending on the LET of the affecting heavy ion.     

An Optimally Integrated Track Recovery System for Aerial Bathymetry

An airborne system has been developed for charting shallow coastal and inland waters. The primary components of this system are an aerial survey camera, a profiling laser radar, an analytical stereo plotter, and a multisensor track recovery system (TRS). The TRS comprises a gimbaled inertial navigation system and a number of auxiliary sensors which acquire redundant position and attitude information. The sensor data are combined postmission using a U-D factorized Kalman filter and modified Bryson-Frazier smoother to compute accurate estimates of the orientation parameters of the survey camera at the times of film exposure. These parameters are used to position each overlapping pair of photographs on the analytical plotter to form a stereo image and corresonding analytical stereomodel from which water depth measurements are made. Flight trial results demonstrate that the TRS can achieve radial position and attitude accuracies which exceed 1 m and 2 arcmin root mean square (rms), respectively, and that this level of performance is sufficient to enable water depth measurements to be made to an accuracy of better than 0.65 m (rms).     

Microgravity and the organisms: results of the Spacelab mission D1.

During the Spacelab mission D1 different organisms were investigated at the unicellular and multicellular level respectively. Microgravity affects growth and development of the organisms in a different manner, some processes are enhanced, others are inhibited. On the other hand, there are a lot of parameters. e.g. circadian rhythm or cell and organ polarity, which seem to be exclusively under genetical control.     

Accessing NASA technology with the World Wide Web

NASA Langley Research Center (LaRC) began using the World Wide Web (WWW) in the summer of 1993, becoming the first NASA installation to provide a center-wide home page. This coincided with a reorganization of LaRC to provide a more concentrated focus on technology transfer to both aerospace and non-aerospace industry. Use of WWW and NCSA Mosaic not only provides automated information dissemination, but also allows for the implementation, evolution and integration of many technology transfer and technology awareness applications. This paper describes several of these innovative applications, including the on-line presentation of the entire Technology Opportunities Showcase (TOPS), an industrial partnering showcase that exists on the Web long after the actual 3-day event ended. The NASA Technical Report Server (NTRS) provides uniform access to many logically similar, yet physically distributed NASA report servers. WWW is also the foundation of the Langley Software Server (LSS), an experimental software distribution system which will distribute LaRC-developed software. In addition to the more formal technology distribution projects, WWW has been successful in connecting people with technologies and people with other people     

The Circular Synthetic Radar

A new type of synthetic radar, the circular synthetic radar, uses a simple interferometer whose elements are mounted at the ends of a horizontal boom rotating about a vertical mast. Pulses are radiated alternately ?in-phase? and in ?phase-quadrature.? The returning echoes are also detected incoherently, both ?in-phase? and in ?phase-quadrature.? The four distinct outputs are fed into an on-line computer which, after a Fourier analysis, synthesizes a mapping function of the azimuthal distribution of targets.     

Closed-Form Expressions for Noncoherent Radar Integration Gain and Collapsing Loss

Closed-form formulas allow rapid determination of noncoherent integration gain and integration loss when the single-sample IF signal-to-noise ratio (SNR) is known. In addition, if the required SNR is known for any number of integrated pulses, the required SNR for any other number is easily determined. A closed-form expression is given for radar collapsing loss, expressed in terms of the equivalent integrated signal-to-noise ratio required to produce a given combination of false-alarm and detection probabilities. Alternatively, the single-sample signal-to-noise ratio of a set of samples may be used together with the closed-form expression for integration gain to get the equivalent integrated signal-to-noise ratio.     

Changes in the amino acid code.

The genetic code is characterized by a pattern arising from "wobble-pairing" between codons and anticodons, so that one nucleotide in the first anticodon position can pair with more than one nucleotide in the third position of a codon. Earlier codes may have existed in which there were fewer anticodons than at present, so that these earlier codes contained fewer amino acids. The universal code was formerly thought to be the only currently existing code used by terrestrial species. It is now known that differences exist from the universal code in mitochondrial coding systems, and also that mitochondrial systems differ from each other. These findings lend support to the proposal that archetypal codes preceded the present universal code. Such archetypal codes may have had some resemblances to mitochondrial codes.