A simplified closed artificial ecosystem--recycling of organic matter into wheat plants.

A simplified closed system consisting of a plant growth chamber coupled to a decomposition chamber was used to study carbon exchange dynamics. The CO2 produced via the decomposition of wheat straw was used for photosynthetic carbon uptake by wheat plants. The atmosphere of the two chambers was connected through a circuit of known flow rate. Thus, monitoring the CO2 concentrations in both compartments allowed measurement of the carbon exchange between the chambers, and estimation of the rate of respiration processes in the decomposition chamber and photosynthetic rate in the producer chamber. The objective for CELSS research was to simulate a system where a compartment producing food via photosynthesis, would be supplied by CO2 produced from respiration processes. The decomposition of biomass by the decomposer simulated both the metabolism of a crew and the result of a recycling system for inedible biomass. Concerning terrestrial ecosystems, the objective was to study organic matter decomposition in soil and other processes related to permanent grasslands.     

Analysis of static loading for a helicopter tubular skid landing gear

We compare the results of analyzing the stress strain state for the tubular skid landing gear with regard for the physically and geometrically nonlinear scheme of deformation; the analysis is carried out by the finite element method and with the aid of a special beam-type design model. The analysis has been performed for a real helicopter design. The peculiarities of deforming the aircraft structures of the type under study are shown based on the comparison results.     

Reanalysis of radiation belt electron phase space density using various boundary conditions and loss models

Data assimilation is becoming an increasingly important tool for understanding the near Earth hazardous radiation environments. Reanalysis of the radiation belts can be used to identify the electron acceleration mechanism and distinguish local acceleration from radial diffusion. However, for any practical applications we need to determine how reliable is reanalysis, and how significant is the dependence of the results on the assumptions of the code and choice of boundary conditions. We present the sensitivity of reanalysis of the radiation belt electron phase space density (PSD) to the assumed location of the outer boundary, using the VERB code and a Kalman filter. We analyze the sensitivity of reanalysis to changes in the electron-loss throughout the domain, and the sensitivity to the assumed boundary condition and its effect on the innovation vector. All the simulations presented in this study for all assumed loss models and boundary conditions, show that peaks in the phase space density of relativistic electrons build up between 4.5 and 6 R_E during relativistic electron flux enhancements in the outer radiation belt. This clearly shows that peaks build up in the heart of the electron radiation belt independent of the assumptions in the model, and that local acceleration is operating there. The work here is also an important step toward performing reanalysis using observations from current and future missions.     

Single ion actions: the induction of micronuclei in V79 cells exposed to individual protons.

Understanding the effects of single-particles from conventional radiation biology experiments is problematic due to the stochastics of particle tracks. This complicates the determinations of risk associated with low doses. We have developed a charged particle microbeam, which allows individually counted particles to be delivered to precise cellular locations. The system is capable of delivering a single charged particle with > 99% efficiency. Of these particles 90% are delivered with a resolution of +/- 2 micrometers and 96% with a resolution of +/- 5 micrometers. We have carried out preliminary studies in Chinese hamster V79 cells to monitor the effectiveness of low energy protons at inducing cytological damage. We have used the micronucleus assay as a measure of predominantly lethal chromosome damage. The effects of a single 3.2 MeV proton delivered individually to cells could be measured, with less than 2% of the exposed cells producing micronuclei 24 hours later. The yield of micronuclei formation was essentially linear up to the highest dose (30 particles per cell nucleus) delivered. Ultimately, the ability to target particles to different parts of the cell nucleus may start to impact on models available for chromosome aberration formation and chromosomal Organisation and mechanisms underlying genomic instability.     

Particulated growth media for optimal liquid and gaseous fluxes to plant roots in microgravity.

An important and yet relatively under researched area of plant growth in microgravity, deals with the rooting environment of plants. A comprehensive approach for selecting the physical characteristics of root growth media which optimizes the dynamic availability of water and dissolved nutrients, and gases to plant roots was developed and tested. Physically-based and parametric models describing the relationship between content and fluxes of liquids and gases were used to cast a multi-objective optimization problem. This methodology reveals that a medium's ability to supply liquid and gas fluxes optimally is dependent upon physiological target values, system operation limits and root module design which dictate the medium's range of soil water characteristic and particle size distribution. Optimized media parameters designate a particle size distribution from which a particulated growth media was constructed and matched to the optimized media parameters. This methodology should improve the selection of optimal media properties for plant growth in microgravity as well as other porous media applications.     

Radiation environment on the Mir orbital station during solar minimum.

The Mir station has been in a 51.65 degrees inclination orbit since March 1986. In March 1995, the first US astronaut flew on the Mir-18 mission and returned on the Space Shuttle in July 1995. Since then three additional US astronauts have stayed on orbit for up to 6 months. Since the return of the first US astronaut, both the Spektr and Priroda modules have docked with Mir station, altering the mass shielding distribution. Radiation measurements, including the direct comparison of US and Russian absorbed dose rates in the Base Block of the Mir station, were made during the Mir-18 and -19 missions. There is a significant variation of dose rates across the core module; the six locations sampled showed a variation of a factor of nearly two. A tissue equivalent proportional counter (TEPC) measured a total absorbed dose rate of 300 microGy/day, roughly equally divided between the rate due to trapped protons from the South Atlantic Anomaly (SAA) and galactic cosmic radiation (GCR). This dose rate is about a factor of two lower than the rate measured by the thinly shielded (0.5 g cm-2 of Al) operational ion chamber (R-16), and about 3/2 of the rate of the more heavily shielded (3.5 g cm-2 of Al) ion chamber. This is due to the differences in the mass shielding properties at the location of these detectors. A comparison of integral linear energy transfer (LET) spectra measured by TEPC and plastic nuclear track detectors (PNTDs) deployed side by side are in remarkable agreement in the LET region of 15-1000 keV/micrometer, where the PNTDs are fully efficient. The average quality factor, using the ICRP-26 definition, was 2.6, which is higher than normally used. There is excellent agreement between the measured GCR dose rate and model calculations, but this is not true for trapped protons. The measured Mir-18 crew skin dose equivalent rate was 1133 microSv/day. Using the skin dose rate and anatomical models, we have estimated the blood-forming organ (BFO) dose rate and the maximum stay time in orbit for International Space Station crew members.     

Specification of the Earth’s plasmasphere with data assimilation

In this paper we report on initial work toward data assimilative modeling of the Earth’s plasmasphere. As the medium of propagation for waves which are responsible for acceleration and decay of the radiation belts, an accurate assimilative model of the plasmasphere is crucial for optimizing the accurate prediction of the radiation environments encountered by satellites. On longer time-scales the plasmasphere exhibits significant dynamics. Although these dynamics are modeled well by existing models, they require detailed global knowledge of magnetospheric configuration which is not always readily available. For that reason data assimilation can be expected to be an effective tool in improving the modeling accuracy of the plasmasphere. In this paper we demonstrate that a relatively modest number of measurements, combined with a simple data assimilation scheme, inspired by the ensemble Kalman filtering data assimilation technique does a good job of reproducing the overall structure of the plasmasphere including plume development. This raises hopes that data assimilation will be an effective method for accurately representing the configuration of the plasmasphere for space weather applications.     

ASTROCULTURE (TM) root metabolism and cytochemical analysis.

Physiology of the root system is dependent upon oxygen availability and tissue respiration. During hypoxia nutrient and water acquisition may be inhibited, thus affecting the overall biochemical and physiological status of the plant. For the Astroculture (TM) plant growth hardware, the availability of oxygen in the root zone was measured by examining the changes in alcohol dehydrogenase (ADH) activity within the root tissue. ADH activity is a sensitive biochemical indicator of hypoxic conditions in plants and was measured in both spaceflight and control roots. In addition to the biochemical enzyme assays, localization of ADH in the root tissue was examined cytochemically. The results of these analyses showed that ADH activity increased significantly as a result of spaceflight exposure. Enzyme activity increased 248% to 304% in dwarf wheat when compared with the ground controls and Brassica showed increases between 334% and 579% when compared with day zero controls. Cytochemical staining revealed no differences in ADH tissue localization in any of the dwarf wheat treatments. These results show the importance of considering root system oxygenation in designing and building nutrient delivery hardware for spaceflight plant cultivation and confirm previous reports of an ADH response associated with spaceflight exposure.     

Spore dosimetry of solar UV radiation: applications to monitoring of daily irradiance and personal exposure.

Environmental UV radiation can be quantified using spore dosimetry, which measures the inactivation of repair-deficient Bacillus subtilis spores dried on a membrane filter. The system exhibits highly selective sensitivity to UV radiation, not being affected by various environmental adversities, such as high and low temperature and humidity. Biologically-effective dose rate and cumulative dose of ambient radiation are measurable under various conditions at various places on the earth, including tropical, temperate, and polar sites. Applications to monitor the exposure at the surface of organisms including humans and plants have also been advanced.     

Six-impulse maneuvers for rendezvous of spacecraft in near-circular noncoplanar orbits

The problem of a rendezvous of two spacecraft in close near-circular noncoplanar orbits is considered. The angles of applying velocity impulses and their orientation are determined from necessary conditions of optimality derived using the basis vector theory. For non-degenerate six-impulse solutions the analytical formulas are found that approximate the dependence of moments of applying velocity impulses and angles determining their orientation on the rendezvous duration. The total characteristic velocity of six-impulse solutions (or five-impulse solutions derived from them) is compared to the total characteristic velocity obtained when solving the Lambert problem.