Clinorotation reduces number, but not size, of cartilaginous nodules formed in micromass cultures of mouse limbbud cells.

In previous studies we used a ground based model to investigate the cellular responses to microgravity by exposing micromass cultures of embryonic limb cells to simulated weightlessness on a clinostat. Cultures set up in T-flasks and rotated at 30 rpm showed that clinostatted cultures had less chondrocyte differentiation than stationary or rotation controls, as assessed by number of nodules/culture stained with cartilage specific Alcian blue. In the current study, nodule size and shape of these nodules was assessed by interactive measurement of area, perimeter, circularity, and equivalent diameters, using the Optimas imaging software. Results show no significant difference in any of the measurements, indicating that clinorotation has no effect on expansion of the nodules either by differentiation of cells within the nodule, or by recruitment of cells into the nodule. The reduction in number of nodules without an alteration in size and shape indicates that the effect of simulated microgravity is to reduce the cell interactions required for the initial condensation of cells into a nodule, probably by interference with cell adhesion molecules.     

Performance limits of sensor-scheduling strategies in electronic support

The case is considered in which a frequency-agile receiver (FAR) for electronic support (ES) attempts to intercept radar emissions over a wide search bandwidth. It was recently shown [1,2] that a random strategy exists in which the expected intercept time can be made arbitrarily close to linear as a function of the scan period of the radar. Can a deterministic strategy be devised in which a similar linear relationship exists for the maximum intercept time? By applying the celebrated arithmetic results of van der Waerden [3] and Szemeredi [4], we show that no such strategy is possible.     

Gravitation and celestial mechanics investigations with Galileo

The gravitation and celestial mechanics investigations during the cruise phase and Orbiter phase of the Galileo mission depend on Doppler and ranging measurements generated by the Deep Space Network (DSN) at its three spacecraft tracking sites in California, Australia, and Spain. Other investigations which also rely on DSN data, and which like ours fall under the general discipline of spacecraft radio science, are described in a companion paper by Howard et al. (1992). We group our investigations into four broad categories as follows: (1) the determination of the gravity fields of Jupiter and its four major satellites during the orbital tour, (2) a search for gravitational radiation as evidenced by perturbations to the coherent Doppler link between the spacecraft and Earth, (3) the mathematical modeling, and by implication tests, of general relativistic effects on the Doppler and ranging data during both cruise and orbiter phases, and (4) an improvement in the ephemeris of Jupiter by means of spacecraft ranging during the Orbiter phase. The gravity fields are accessible because of their effects on the spacecraft motion, determined primarily from the Doppler data. For the Galilean satellites we will determine second degree and order gravity harmonics that will yield new information on the central condensation and likely composition of material within these giant satellites (Hubbard and Anderson, 1978). The search for gravitational radiation is being conducted in cruise for periods of 40 days centered around solar opposition. During these times the radio link is least affected by scintillations introduced by solar plasma. Our sensitivity to the amplitude of sinusoidal signals approaches 10^-15 in a band of gravitational frequencies between 10^-4 and 10^-3 Hz, by far the best sensitivity obtained in this band to date. In addition to the primary objectives of our investigations, we discuss two secondary objectives: the determination of a range fix on Venus during the flyby on 10 February, 1990, and the determination of the Earth's mass (GM) from the two Earth gravity assists, EGA1 in December 1990 and EGA2 in December 1992.     

On-board statistical detection and compensation of anomalous pilot-aircraft interactions

A statistical method for the on-board detection and compensation of adverse oscillations in pilot-aircraft systems is presented. A recursively updated linear model representing the pilot-aircraft system is used to continuously check for stability loss. The instability-related oscillations are detected early, using a statistical hypothesis test. Furthermore, a specially designed stability augmentation system assists the pilot during the instability periods. The method's effectiveness is demonstrated via data obtained from a flight simulator and a detailed simulation model.     

The characteristics of sharp (small-scale) boundaries of solar wind plasma and magnetic field structures

We investigate properties of large (>20%) and sharp (<10 min) solar wind ion flux changes using INTERBALL-1 and WIND plasma and magnetic field measurements from 1996 to 1999. These ion flux changes are the boundaries of small-scale and middle-scale solar wind structures. We describe the behavior of the solar wind velocity, temperature and interplanetary magnetic field (IMF) during these sudden flux changes. Many of the largest ion flux changes occur during periods when the solar wind velocity is nearly constant, so these are mainly plasma density changes. The IMF magnitude and direction changes at these events can be either large or small. For about 55% of the ion flux changes, the sum of the thermal and magnetic pressure are in balance across the boundary. In many of the other cases, the thermal pressure change is significantly more than the magnetic pressure change. We also attempted to classify the types of discontinuities observed.