Gravisensing: ionic responses, cytoskeleton and amyloplast behavior.

In Zea mays L., changes in orientation of stems are perceived by the pulvinal tissue, which responds to the stimulus by differential growth resulting in upward bending of the stem. Gravity is perceived in the bundle sheath cells, which contain amyloplasts that sediment to the new cell base when a change in the gravity vector occurs. The mechanism by which the mechanical signal is transduced into a physiological response is so far unknown for any gravity perceiving tissue. It is hypothesized that this involves interactions of amyloplasts with the plasma membrane and/or ER via cytoskeletal elements. To gain further insights into this process we monitored amyloplast movements in response to gravistimulation. In a pharmacological approach we investigated how the dynamics of plastid sedimentation are affected by actin and microtubule (MT) disrupting drugs. Dark grown caulonemal filaments of the moss Physcomitrella patens respond to gravity vector changes with a reorientation of tip growth away from the gravity vector. MT distributions in tip cells were monitored over time and MTs were seen to accumulate preferentially on the lower flank of the tip 30 min after a 90 degree turn. Using a self-referencing Ca2+ selective ion probe, we found that growing caulonemal filaments exhibit a Ca2+ influx at the apical dome, similar to that reported previously for other tip growing cells. However, in gravistimulated Physcomitrella filaments the region of Ca2+ influx is not confined to the apex, but extends about 60 micrometers along the upper side of the filament. Our results indicate an asymmetry in the Ca2+ flux pattern between the upper and side of the filament suggesting differential activation of Ca2+ permeable channels at the plasma membrane.     

Analysis of the Cyclotron Facility calibration and aircraft dosimetry results from the LIULIN-3M instrument.

The LIULIN-3M instrument is a further development of the LIULIN dosimeter-radiometer, used on the MIR spacestation during the 1988-1994 time period. The LIULIN-3M is designed for continuous monitoring of the radiation environment during the BION-12 satellite flight in 1999. A semiconductor detector with 1 mm thickness and cm2 area is contained in the instrument. Pulse high analysis technique is used to determine the energy losses in the detector. The final data from the instrument are the flux and the dose rate for the exposure time and 256 channels of absorbed dose spectra based on the assumption that the particle flux is normal to the detector. The LIULIN-3M instrument was calibrated by proton fluxes with different energies at the Indiana University Cyclotron Facility in June 1997 and had been used for radiation measurements during commercial aircraft flights. The calibration procedure and some flight results are presented in this paper.     

Modeling of ionospheric parameter variations in East Asia during the moderate geomagnetic disturbances

The results of modeling of ionospheric disturbances observed in the East Asian region during moderate storms are presented. The numerical model for ionosphere–plasmasphere coupling developed at the ISTP SB RAS is used to interpret the data of observations at ionospheric stations located in the longitudinal sector of 90–130°E at latitudes from auroral zone to equator. There is obtained a reasonable agreement between measurements and modeling results for winter and equinox. In the summer ionosphere, at the background of high ionization by the solar EUV radiation in the quiet geomagnetic period the meridional thermospheric wind strongly impacts the electron concentration in the middle and auroral ionosphere. The consistent calculations of the thermospheric wind permit to obtain the model results which are closer to summer observations. The actual information about the space-time variations of thermosphere and magnetosphere parameters should be taken into account during storms.     

Particle trajectories in seeds of Lactuca sativa and chromosome aberrations after exposure to cosmic heavy ions on Cosmos Biosatellites 8 and 9.

The potentially specific importance of the heavy ions of the galactic cosmic radiation for radiation protection in manned spaceflight continues to stimulate in situ, i.e., spaceflight experiments to investigate their radiobiological properties. Chromosome aberrations as an expression of a direct assault on the genome are of particular interest in view of cancerogenesis being the primary radiation risk for man in space. In such investigations the establishment of the geometrical correlation between heavy ions' trajectories and the location of radiation sensitive biological substructures is an essential task. The overall qualitative and quantitative precision achieved for the identification of particle trajectories in the order of approximately 10 micrometers as well as the contributing sources of uncertainties are discussed. We describe how this was achieved for seeds of Lactuca sativa as biological test organisms, whose location and orientation had to be derived from contact photographies displaying their outlines and those of the holder plates only. The incidence of chromosome aberrations in cells exposed during the COSMOS 1887 (Biosatellite 8) and the COSMOS 2044 (Biosatellite 9) mission was determined for seeds hit by cosmic heavy ions. In those seeds the incidence of both single and multiple chromosome aberrations was enhanced. The results of the Biosatellite 9 experiment, however, are confounded by spaceflight effects unrelated to the passage of heavy ions.     

Oxygen Isotopes in the Solar System

The oxygen three-isotope system has major advantages over the two-isotope systems of hydrogen, carbon, and nitrogen because different fractionation laws govern intraplanetary and interplanetary processes. This permits discriminating between solar nebular processes and parent-body processes. Oxygen isotopes also serve as a sensitive natural tracer for meteorite classification. This revised version was published online in August 2006 with corrections to the Cover Date.     

A program to measure new energetic particle nuclear interaction cross sections.

The Transport Collaboration, consisting of researchers from institutions in France, Germany, Italy and the USA, has established a program to make new measurements of nuclear interaction cross sections for heavy projectiles (Z > or = 2) in targets of liquid H2, He and heavier materials. Such cross sections directly affect calculations of galactic and solar cosmic ray transport through matter and are needed for accurate radiation hazard assessment. To date, the collaboration has obtained data using the LBL Bevalac HISS facility with 20 projectiles from 4He to 58Ni in the energy range 393-910 MeV/nucleon. Preliminary results from the analysis of these data are presented here and compared to other measurements and to cross section prediction formulae.     

A two-dimensional position-sensitive spectroscopic proportional counter for balloon-borne hard X-ray astronomy

A new design of position sensitive spectroscopic proportional counter is described, for use in a balloon borne hard x-ray telescope. Initial position and spectral resolution data from a one-dimensional laboratory prototype are reported. With this device, the final telescope will have an angular resolution of better than 10 minutes of arc.     

Investigation of natural and artificial stimulation of the ionospheric Alfvén resonator at high latitude

A brief review is provided of recent progress in understanding the ionospheric Alfvén resonator (IAR) at high latitude. Firstly, naturally occurring resonances of the IAR as detected by pulsation magnetometers in the auroral zone at Sodankylä and in the polar cap at Barentsburg are considered. The characteristics of the IAR in the two regions are broadly similar, although the effects of solar illumination are less clear at the higher latitudes. Secondly we review recent attempts to stimulate the IAR through high-power radio frequency experiments both in the auroral zone at Tromsø with the European Incoherent SCATter (EISCAT) heater, and within the polar cap at Longyearbyen with the Space Plasma Exploration by Active Radar (SPEAR) facility. In the auroral zone at, Tromsø the stimulated IAR has been observed by ground-based magnetometers, and through electron acceleration observed on the FAST spacecraft. At SPEAR in the polar cap, the stimulated IAR has been investigated, with ground magnetometers, with the first results indicative of a positive detection.