Carotenoid analysis of halophilic archaea by resonance Raman spectroscopy

Recently, halite and sulfate evaporate rocks have been discovered on Mars by the NASA rovers, Spirit and Opportunity. It is reasonable to propose that halophilic microorganisms could have potentially flourished in these settings. If so, biomolecules found in microorganisms adapted to high salinity and basic pH environments on Earth may be reliable biomarkers for detecting life on Mars. Therefore, we investigated the potential of Resonance Raman (RR) spectroscopy to detect biomarkers derived from microorganisms adapted to hypersaline environments. RR spectra were acquired using 488.0 and 514.5 nm excitation from a variety of halophilic archaea, including Halobacterium salinarum NRC-1, Halococcus morrhuae, and Natrinema pallidum. It was clearly demonstrated that RR spectra enhance the chromophore carotenoid molecules in the cell membrane with respect to the various protein and lipid cellular components. RR spectra acquired from all halophilic archaea investigated contained major features at approximately 1000, 1152, and 1505 cm(-1). The bands at 1505 cm(-1) and 1152 cm(-1) are due to in-phase C=C (nu(1) ) and C-C stretching ( nu(2) ) vibrations of the polyene chain in carotenoids. Additionally, in-plane rocking modes of CH(3) groups attached to the polyene chain coupled with C-C bonds occur in the 1000 cm(-1) region. We also investigated the RR spectral differences between bacterioruberin and bacteriorhodopsin as another potential biomarker for hypersaline environments. By comparison, the RR spectrum acquired from bacteriorhodopsin is much more complex and contains modes that can be divided into four groups: the C=C stretches (1600-1500 cm(-1)), the CCH in-plane rocks (1400-1250 cm(-1)), the C-C stretches (1250-1100 cm(-1)), and the hydrogen out-of-plane wags (1000-700 cm(-1)). RR spectroscopy was shown to be a useful tool for the analysis and remote in situ detection of carotenoids from halophilic archaea without the need for large sample sizes and complicated extractions, which are required by analytical techniques such as high performance liquid chromatography and mass spectrometry.     

An endogenous growth pattern of roots is revealed in seedlings grown in microgravity

In plants, sensitive and selective mechanisms have evolved to perceive and respond to light and gravity. We investigated the effects of microgravity on the growth and development of Arabidopsis thaliana (ecotype Landsberg) in a spaceflight experiment. These studies were performed with the Biological Research in Canisters (BRIC) hardware system in the middeck region of the space shuttle during mission STS-131 in April 2010. Seedlings were grown on nutrient agar in Petri dishes in BRIC hardware under dark conditions and then fixed in flight with paraformaldehyde, glutaraldehyde, or RNAlater. Although the long-term objective was to study the role of the actin cytoskeleton in gravity perception, in this article we focus on the analysis of morphology of seedlings that developed in microgravity. While previous spaceflight studies noted deleterious morphological effects due to the accumulation of ethylene gas, no such effects were observed in seedlings grown with the BRIC system. Seed germination was 89% in the spaceflight experiment and 91% in the ground control, and seedlings grew equally well in both conditions. However, roots of space-grown seedlings exhibited a significant difference (compared to the ground controls) in overall growth patterns in that they skewed to one direction. In addition, a greater number of adventitious roots formed from the axis of the hypocotyls in the flight-grown plants. Our hypothesis is that an endogenous response in plants causes the roots to skew and that this default growth response is largely masked by the normal 1?g conditions on Earth.     

非对称多站在目标跟踪中的精度几何递变

现有靶场测量系统的笛氏坐标精确度估计取决于目标的位置以及测量仪器的数量、位置和精确度等因素。本报告分析了测量仪器的非对称布站问题。这是以往研究成果的推广,并且处理了更为一般性的问题。研究结果证明,对于测角仪器,当测站布局为非对称时,误差估计与所用的方位参考系有一定的关系。导出了几类布站几何的误差表达式,并为某类布站几何找出了最优的几何布站方案。     

Spacelab experiments on space motion sickness

Recent research results from ground and flight experiments on motion sickness and space sickness conducted by the Man Vehicle Laboratory are reviewed. New tools developed include a mathematical model for motion sickness, a method for quantitative measurements of skin pallor and blush in ambulatory subjects, and a magnitude estimation technique for ratio scaling of nausea or discomfort. These have been used to experimentally study the time course of skin pallor and subjective symptoms in laboratory motion sickness. In prolonged sickness, subjects become hypersensitive to nauseogenic stimuli. Results of a Spacelab-1 flight experiment are described in which four observers documented the stimulus factors for and the symptoms/signs of space sickness. The clinical character of space sickness differs somewhat from acute laboratory motion sickness. However SL-1 findings support the view that space sickness is fundamentally a motion sickness. Symptoms were subjectively alleviated by head movement restriction, maintenance of a familiar orientation with respect to the visual environment, and wedging between or strapping onto surfaces which provided broad contact cues confirming the absence of body motion.     

Properties of an Earth-like planet orbiting a Sun-like star: Earth observed by the EPOXI mission

NASA's EPOXI mission observed the disc-integrated Earth and Moon to test techniques for reconnoitering extrasolar terrestrial planets, using the Deep Impact flyby spacecraft to observe Earth at the beginning and end of Northern Hemisphere spring, 2008, from a range of ～1/6 to 1/3 AU. These observations furnish high-precision and high-cadence empirical photometry and spectroscopy of Earth, suitable as "ground truth" for numerically simulating realistic observational scenarios for an Earth-like exoplanet with finite signal-to-noise ratio. Earth was observed at near-equatorial sub-spacecraft latitude on 18-19 March, 28-29 May, and 4-5 June (UT), in the range of 372-4540?nm wavelength with low visible resolving power (λ/Δλ=5-13) and moderate IR resolving power (λ/Δλ=215-730). Spectrophotometry in seven filters yields light curves at ～372-948?nm filter-averaged wavelength, modulated by Earth's rotation with peak-to-peak amplitude of ≤20%. The spatially resolved Sun glint is a minor contributor to disc-integrated reflectance. Spectroscopy at 1100-4540?nm reveals gaseous water and carbon dioxide, with minor features of molecular oxygen, methane, and nitrous oxide. One-day changes in global cloud cover resulted in differences between the light curve beginning and end of ≤5%. The light curve of a lunar transit of Earth on 29 May is color-dependent due to the Moon's red spectrum partially occulting Earth's relatively blue spectrum. The "vegetation red edge" spectral contrast observed between two long-wavelength visible/near-IR bands is ambiguous, not clearly distinguishing between the verdant Earth diluted by cloud cover versus the desolate mineral regolith of the Moon. Spectrophotometry in at least one other comparison band at short wavelength is required to distinguish between Earth-like and Moon-like surfaces in reconnaissance observations. However, measurements at 850?nm alone, the high-reflectance side of the red edge, could be sufficient to establish periodicity in the light curve and deduce Earth's diurnal period and the existence of fixed surface units.     

速度状态参数的最优观测几何

本文指出飞行器速度状态参数的测量也存在最优观测几何。对三维球面测速定位系统分析的结果是:测量飞行器速度状态参数的最优布站几何与测量飞行器位置状态参数的最优布站几何完全一致。基线长度则分两种情况,由速度观测元素(距离变化率R)误差传播系数ε_(V1)导出的最优基线长度与测量飞行器位置状态参数的最优基线长度完全一致;而由位置观测元素(距离R)误差传播系数ε_(V2)导出的最优基线长度只在满足β=(x~2 z~2)/y~2<1.5的条件下存在。同时,飞行器速度矢量的模以正比例关系影响位置观测元素误差传播系数的值,速度矢量的方向只影响笛氏坐标系中各速度分量间的精度分配值。     

远方跟踪站所用的小型计算机程序

现在已编制出一种远方跟踪站所用的小型计算机程序,它每秒钟执行100次,并对方位角和俯仰角进行平滑与外推。指向角误差一般小于0.01度,距离精确度在1米以内。