Entrapment of bacteria in fluid inclusions in laboratory-grown halite

Cells of the bacterium Pseudomonas aeruginosa, which were genetically modified to produce green fluorescent protein, were entrapped in fluid inclusions in laboratory-grown halite. The bacteria were used to inoculate NaCl-saturated aqueous solutions, which were allowed to evaporate and precipitate halite. The number, size, and distribution of fluid inclusions were highly variable, but did not appear to be affected by the presence of the bacteria. Many of the inclusions in crystals from inoculated solutions contained cells in populations ranging from two to 20. Microbial attachment to crystal surfaces was neither evident nor necessary for entrapment. Cells occurred exclusively within fluid inclusions and were not present in the crystal matrix. In both the inclusions and the hypersaline solution, the cells fluoresced and twitched, which indicates that the bacteria might have remained viable after entrapment. The fluorescence continued up to 13 months after entrapment, which indicates that little degradation of the bacteria occurred over that time interval. The entrapment, fluorescence, and preservation of cells were independent of the volume of hypersaline solution used or whether the solutions were completely evaporated prior to crystal extraction. The results of this study have a wide range of implications for the long-term survival of microorganisms in fluid inclusions and their detection through petrography. The results also demonstrate the preservation potential for microbes in hypersaline fluid inclusions, which could allow cells to survive harsh conditions of space, the deep geologic past, or burial in sedimentary basins.     

Influence on photosynthesis of starlight, moonlight, planetlight, and light pollution (reflections on photosynthetically active radiation in the universe)

Photosynthesis on Earth can occur in a diversity of organisms in the photosynthetically active radiation (PAR) range of 10 nmol of photons m(-2) s(-1) to 8 mmol of photons m(-2) s(-1). Similar considerations would probably apply to photosynthetic organisms on Earth-like planets (ELPs) in the continuously habitable zone of other stars. On Earth, starlight PAR is inadequate for photosynthetically supported growth. An increase in starlight even to reach the minimum theoretical levels to allow for photosynthesis would require a universe that was approximately ten million times older, or with a ten million times greater density of stars, than is the case for the present universe. Photosynthesis on an ELP using PAR reflected from a natural satellite with the same size as our Moon, but at the Roche limit, could support a low rate of photosynthesis at full Moon. Photosynthesis on an ELP-like satellite of a Jupiter-sized planet using light reflected from the planet could be almost 1% of the rate in full sunlight on Earth when the planet was full. These potential contributions to photosynthesis require that the contribution is compared with the rate of photosynthesis driven by direct radiation from the star. Light pollution on Earth only energizes photosynthesis by organisms that are very close to the light source. However, effects of light pollution on photosynthesis can be more widespread if the photosynthetic canopy is retained for more of the year, caused by effects on photoperiodism, with implications for the influence of civilizations on photosynthesis.     

Ranging airport pseudolite for local area augmentation

This paper discusses the integration of an airport pseudolite (APL) into a local area augmented differential GPS based precision approach system. A prototype architecture is described that is being used to develop requirements for the local area augmentation system. Key features of this prototype system are presented along with its current performance. Key features discussed include the use of a multipath limiting antenna, APL signal structure factors, a unique APL automatic gain control, and GPS blanking technique to maximize APL tracking performance, while minimizing the electromagnetic interference to nominal DGPS performance     

Solar Variability and the Earth's Climate: Introduction and Overview

Numerous attempts have been made over the years to link various aspects of solar variability to changes in the Earth's climate. There has been growing interest in this possible connection in recent years, spurred largely by the need to understand the natural causes of climate change, against which the expected global warming due to man's activities will have to be detected. The time scale of concern here is that of decades to centuries, and excludes the longer millennial scale in which orbital variations play a dominant role. The field has long been plagued by the lack of an acceptable physical mechanism by which solar variability can affect climate, but the discovery of variability in the Sun's total irradiance (the solar ``constant' of meteorology) by spacecraft instruments has pointed to a direct mechanism. Other less direct mechanisms that have been suggested involve variations in the Sun's ultraviolet flux and in the plasma outflow of the solar wind. The purpose of this paper is to summarize the current state of the field, emphasizing the proposed mechanisms as an introduction to the more detailed papers that follow. The particular case of sea-surface temperature data will be used as an illustration.     

Shadow detection for mobile robots: Features,evaluation, and datasets

ABSTRACT

Shadows have long been a challenging topic for computer vision. This challenge is made even harder when we assume that the camera is moving, as many existing shadow detection techniques require the creation and maintenance of a background model. This article explores the problem of shadow modelling from a moving viewpoint (assumed to be a robotic platform) through comparing shadow-variant and shadow-invariant image features — primarily color, texture and edge-based features. These features are then embedded in a segmentation pipeline that provides predictions on shadow status, using minimal temporal context. We also release a public dataset of shadow-related image sequences, to help other researchers further develop shadow detection methods and to enable benchmarking of techniques.     

Post-midnight equatorial irregularity distributions and vertical drift velocity variations during solstices

Longitudinal distributions of post-midnight equatorial ionospheric irregularity occurrences observed by ROCSAT-1 (1st satellite of the Republic of China) during moderate to high solar activity years in two solstices are studied with respect to the vertical drift velocity and density variations. The post-midnight irregularity distributions are found to be similar to the well-documented pre-midnight ones, but are different from some published distributions taken during solar minimum years. Even though the post-midnight ionosphere is sinking in general, longitudes of frequent positive vertical drift and high density seems to coincide with the longitudes of high irregularity occurrences. Large scatters found in the vertical drift velocity and density around the dip equator in different ROCSAT-1 orbits indicate the existence of large and frequent variations in the vertical drift velocity and density that seem to be able to provide sufficient perturbations for the Rayleigh-Taylor (RT) instability to cause the irregularity occurrences. The need of seeding agents such as gravity waves from atmospheric convective clouds to initiate the Rayleigh-Taylor instability may not be necessary.     

The Venus ionosphere

Physical properties of the Venus ionosphere obtained by experiments on the US Pioneer Venus and the Soviet Venera missions are presented in the form of models suitable for inclusion in the Venus International Reference Atmosphere. The models comprise electron density (from 120 km), electron and ion temperatures, and relative ion abundance in the altitude range from 150 km to 1000 km for solar zenith angles from 0° to 180°. In addition, information on ion transport velocities, ionopause altitudes, and magnetic field characteristics of the Venus ionosphere, are presented in tabular or graphical form. Also discussed is the solar control of the physical properties of the Venus ionosphere.     

Detection of suprathermal ionospheric O+ ions inside the plasmasphere

The plasma diagnostic experiments on the AUREOL-3 satellite have revealed flows of low energy 0⁺ ions deep inside the night plasmasphere during a large substorm. Flux gradients of the 0⁺ ions were accompanied by enhancements of ELF electric field noise. The appearance of suprathermal ions at $\text{L ? 2.5 – 3}$ is interpreted within the framework of electrostatic ion-cyclotron acceleration of ionospheric ions in the diffuse auroral zone /12/ followed by a radial displacement of these ions inside the plasmasphere driven by azimuthal electric fields during substorm activity. Electrostatic oscillations observed inside the plasmasphere are apparently associated with gradient instability at the sharp boundaries of suprathermal ion flows.     

Stability Analysis of a Buck Regulator Employing Input Filter Compensation

The interaction between the input filter and the regulator often causes serious degradation of performance. The reduction in loop gain due to input filter interaction can result in system instability. An exact stability analysis of the buck regulator system is presented. The input filter parameter values are varied and system instability is predicted for the case without feedforward. The eigenvalues of the system can be brought back into the unit circle and the system thus stabilized with the addition of the feedforward loop. Measurements made for the cases with and without feedforward confirm the analytical prediction.     

Project Trinidad

The first intercontinental satellite communication link, which involved the NASA Echo I balloon, a transmitting station on the Island of Trinidad, and a receiving station at Floyd, NY, is discussed. Rome Air Development Center (RADC) experimental stations were set up using a great deal of ingenuity and makeshift equipment and on a very small budget. Much of the equipment was salvaged from unrelated systems, while some was hand-built for this project. The implementation of the system, the testing of the system using moon bounce, the first orbital launch of the Echo I balloon, and the early difficulties encountered in the project are reviewed