Neoplastic cell transformation by high-LET radiation: molecular mechanisms.

Experimental data on molecular mechanisms are essential for understanding the bioeffects of radiation and for developing biophysical models, which can help in determining the shape of dose-response curves at very low doses, e.g., doses less than 1 cGy. Although it has been shown that ionizing radiation can cause neoplastic cell transformation directly, that high-LET heavy ions in general can be more effective than photons in transforming cells, and that the radiogenic cell transformation is a multi-step process [correction of processes], we know very little about the molecular nature of lesions important for cell transformation, the relationship between lethal and transformational damages, and the evolution of initial damages into final chromosomal aberrations which alter the growth control of cells. Using cultured mouse embryo cells (C3H10T1/2) as a model system, we have collected quantitative data on dose-response curves for heavy ions with various charges and energies. An analysis of these quantitative data suggested that two DNA breaks formed within 80 angstroms may cause cell transformation and that two DNA breaks formed within 20 angstroms may be lethal. Through studies with restriction enzymes which produce DNA damages at specific sites, we have found that DNA double strand breaks, including both blunt- and cohesive-ended breaks, can cause cell transformation in vitro. These results indicate that DNA double strand breaks can be important primary lesions for radiogenic cell transformation and that blunt-ended double strand breaks can form lethal as well as transformational damages due to misrepair or incomplete repair in the cell. The RBE-LET relationship is similar for HGPRT gene mutation, chromosomal deletion, and cell transformation, suggesting common lesions may be involved in these radiation effects. The high RBE of high-LET radiation for cell killing and neoplastic cell transformation is most likely related to its effectiveness in producing DNA double strand breaks in mammalian cells. At present the role of oncogenes in radiation cell transformation is unclear.     

Software intensive systems safety analysis

Two important elements in the avionics suite of modern aircraft are: the flight control system (FCS) and the flight management system (FMS). The FCS provides the capability to stabilize and control the aircraft, while the FMS is responsible for flight planning and navigation. A clear trend in the aerospace industry is to place greater reliance on software systems, and many FCS and FMS subsystems are implemented primarily in software. For example, within the FCS is the flight guidance system (FGS) that generates roll and pitch guidance commands. Similarly, within the FMS is the vertical navigation (VNAV) function that acts like a third crew member in the cockpit, ordering mode change requests and resetting target altitude values to enable the aircraft to track the vertical flight plan. We have developed formal, executable models of the requirements for the mode logic of a FGS and for portions of the VNAV functionality. We have also conducted a comprehensive software safety analysis on the FGS mode logic model, and are completing the analysis of the VNAV model. This analysis uses as its starting point several "traditional" safety analysis techniques such as a functional hazard assessment (FHA), a fault tree analysis (FTA), and a failure mode effects analysis (FMEA). However, we are also using formal methods techniques known as model checking and theorem proving to verify the presence of safety properties in the model. This paper summarizes the (now completed) safety analysis that was performed on the FGS model, and highlights the similarities and differences with the (still on-going) safety analysis of the FMS model. In particular, we summarize progress made to date in the use of formal methods to verify the presence of the required safety properties in the models themselves.     

Range Contamination and Its Effect on Measurements

Increased sensitivity and dynamic range of the instrumental techniques used in conjunction with experiments on ballistic ranges have brought to the fore many problems arising from contamination in the ranges themselves. This is seldom discussed when experimental results are presented but is frequently the controlling limitation on the accuracy of the measurements. The authors discuss contamination due to dirt and debris resultant from gun operation, gaseous impurities, and projectile-borne impurities as they have occurred at the Re-entry Simulating Range of Lincoln Laboratory. The effects of these contaminants on measurements are discussed and illustrated, and measures for controlling them are outlined. Finally, a particular range operation is described from the standpoint of impurity control.     

The role of soil science in space exploration

New frontiers in soil science are currently found in the investigation of soils in harsh, terrestrial and simulated extraterrestrial environments, the development of new methods and probes for soil characterization, and the eventual investigation, characterization, and development of extraterrestrial soil. Current aspects of space science involving soil studies are presented, including a more detailed soil study program involving the microflora of desert soil ecosystems. Basic precepts are given for preparation, investigation, and use of extraterrestrial soil.Contribution from the Bioscience Group of the Chemistry Section, Space Science Division, Jet Propulsion Laboratory, California Institute of Technology, Pasadena, California. Presented as an invitational paper before the Synapsis Club, University of California, Riverside, California, December 3, 1962.     

Optimization of entry-vehicle shapes during conceptual design

During the conceptual design of a re-entry vehicle, the vehicle shape and geometry can be varied and its impact on performance can be evaluated. In this study, the shape optimization of two classes of vehicles has been studied: a capsule and a winged vehicle. Their aerodynamic characteristics were analyzed using local-inclination methods, automatically selected per vehicle segment. Entry trajectories down to Mach 3 were calculated assuming trimmed conditions. For the winged vehicle, which has both a body flap and elevons, a guidance algorithm to track a reference heat-rate was used. Multi-objective particle swarm optimization was used to optimize the shape using objectives related to mass, volume and range. The optimizations show a large variation in vehicle performance over the explored parameter space. Areas of very strong non-linearity are observed in the direct neighborhood of the two-dimensional Pareto fronts. This indicates the need for robust exploration of the influence of vehicle shapes on system performance during engineering trade-offs, which are performed during conceptual design. A number of important aspects of the influence of vehicle behavior on the Pareto fronts are observed and discussed. There is a nearly complete convergence to narrow-wing solutions for the winged vehicle. Also, it is found that imposing pitch-stability for the winged vehicle at all angles of attack results in vehicle shapes which require upward control surface deflections during the majority of the entry.     

Broadband light source for fiber-optic measurement system in spaceborne applications

Measuring temperatures, mechanical loads and derived quantities precisely and reliably play an important role in spaceflight. With spacecraft becoming increasingly complex, upscaling of present telemetry techniques can become cumbersome. Additionally, there are entirely new sensory requirements, resulting from emerging technologies such as smart structures, active vibration damping and composite material health monitoring. It has been demonstrated in preceding studies that these measurements can be advantageously and efficiently carried out by means of fiber-optic systems. The most prominent fiber-optic strain and temperature sensor is the fiber Bragg grating. Typically, multiple fiber Bragg gratings are used to translate entire temperature and strain fields into an optical wavelength information. For the interrogation of these sensors, a broadband or scanning light source is required. Additional requirements with respect to the light source are high intensity and unpolarized illumination of the gratings. These constraints can be met by a light source that is based on amplified spontaneous emission in a rare-earth-doped fiber. In the presented work, a compact light source, adapted for measurement applications and targeted towards space applications, has been developed. The design of this light source is presented, as well as its implementation. The light source has been designed and tested for selected core aspects of space robustness and the results of these tests are summarized.     

Cosmic Rays From Supernova Remnants: a Brief Description of the Shock Acceleration of gas and Dust

Space Science Reviews - We summarize our model of galactic cosmic-ray (GCR) origin and acceleration, wherein a mixture of interstellar and/or circumstellar gas and dust is accelerated by a...     

Variations of ionospheric fluctuations of phase delay depending on solar activity: Data of the COSMIC experiment

We present the results of processing and analysis of more than 4500 events of radio occultation sounding of the Earth’s atmosphere observed in the course of the COSMIC experiment on the limb path ‘satellite-ionosphere-satellite’. Events observed in December 2011 (when a number of solar flares occurred) and in January 2012 (when a strong solar proton event took place) were analyzed. It is shown that small-scale variations of electron density increase in polar latitudes, equatorial region, and midlatitudes of the southern hemisphere in January 2012. In the same period, an increase of large-scale variations of electron density is observed during daylight hours in the equatorial region and in the southern hemisphere. No noticeable distinctions in comparison with days of quiet Sun were observed in December 2011.