Long clinostation influence on the localization of free and weakly bound calcium in cell walls of Funaria hygrometrica moss protonema cells.

The pyroantimonate method was used to study the localization of free and weakly bound calcium in cells of moss protonema of Funaria hygrometrica Hedw. cultivated on a clinostat (2rev/min). Electroncytochemical study of control cells cultivated at 1 g revealed that granular precipitate marked chloroplasts, mitochondria, Golgi apparatus, lipid drops, nucleoplasma, nucleolus, nucleus membranes, cell walls and endoplasmic reticulum. In mitochondria the precipitate was revealed in stroma, in chloroplast it was found on thylakoids and envelope membranes. The cultivation of protonema on clinostat led to the intensification in cytochemical reaction product deposit. A considerable intensification of the reaction was noted in endomembranes, vacuoles, periplasmic space and cell walls. At the same time analysis of pectinase localization was made using the electroncytochemical method. A high reaction intensity in walls in comparison to that in control was found out to be a distinctive peculiarity of the cells cultivated on clinostat. It testifies to the fact that increasing of free calcium concentrations under conditions of clinostation is connected with pectinic substances hydrolysis and breaking of methoxy groups of pectins. Data obtained are discussed in relation to problems of possible mechanisms of disturbance in calcium balance of plant cells and the role of cell walls in gomeostasis of cell grown under conditions of simulated weightlessness.     

Interpretation of data on laser sensing of aerosol atmosphere from space

A single-frequency lidar, using aerosol scattering as an informative component, is the simplest and reliable facility for remote sensing of the atmosphere. The information on vertical distribution of atmospheric aerosol which can be obtained using such a lidar is necessary for investigating the physics of atmospheric processes and forecast of optical state of the atmosphere. At the same time, the interpretation of data on single-frequency sounding is associated with some difficulties of fundamental character, mainly due to insufficient software of the experiment. Under such conditions the problems of optimal processing of lidar returns aiming at extracting the useful information on aerosol are of great importance, especially if one takes into account the hindering effects of atmospheric background and optical noises. This paper presents a statistical approach to this problem, and the possibilities of single-frequency sounding from space are analyzed.     

Phase separated membrane bioreactor: results from model system studies.

The operation and evaluation of a bioreactor designed for high intensity oxygen transfer in a microgravity environment is described. The reactor itself consists of a zero headspace liquid phase separated from the air supply by a long length of silicone rubber tubing through which the oxygen diffuses in and the carbon dioxide diffuses out. Mass transfer studies show that the oxygen is film diffusion controlled both externally and internally to the tubing and not by diffusion across the tube walls. Methods of upgrading the design to eliminate these resistances are proposed. Cell growth was obtained in the fermenter using Saccharomyces cerevisiae showing that this concept is capable of sustaining cell growth in the terrestrial [correction of terrestial] simulation.     

An empirical model of electron density for low and middle latitudes below 200 km

Our empirical model of electron density (n_e) for quiet and weakly disturbed geomagnetic conditions (Kp not greater 4) takes account of comparative analysis of existing models and of experimental data obtained by rockets and incoherent scatter radar. The model describes the n_e distribution in the 80 to 200 km height range at low and middle latitudes, and to some extent, in the subauroral region. It is presented in analytical form thus allowing one to calculate electron density profiles for any time. The electron density distribution at 140 km depends on the season (day of the year) and on the solar zenith angle. Profile variations during the day are for one season shown. Different from other models, ours specifies the variations during sunrise and sunset and reflects the particular profile shape at night admitting the occurrence of an intermediate layer.     

Soft X-ray characteristics of white dwarfs observed by EXOSAT

EXOSAT has observed 19 hot white dwarfs with alleged strong soft X-ray emission. Positive detection of a large fraction of this sample was obtained, among these practically all hot DA dwarfs. High-resolution spectral data, acquired with the 500 1/mm grating spectrometer, indicates no traces of He in the atmosphere of HZ43, i.e. n(He)/n(H) ? 10^?5 at a photospheric temperature of 60000 K (log g = 8). In contrast, the hot DA1 dwarf Feige 24 shows the presence of an appreciable He-abundance (n(He)/n(H) ? 10^?3); however no simple homogeneously mixed H/He atmosphere can explain the observed spectral shape.     

Circulation of energetic ions of terrestrial origin in the magnetosphere

Energetic ion composition measurements have now been performed from earth orbiting satellites for more than a decade. As early as 1972 we knew that energetic (keV) ions of terrestrial origin represented a non-negligible component of the storm time ring current. We have now assembled a significant body of knowledge concerning energetic ion composition throughout much of the earth's magnetosphere. We know that terrestrial ions are a common component of the hot equatorial magnetospheric plasma in the ring current and the plasma sheet out to ? 23 R_E. During periods of enhanced geomagnetic activity this component may become dominant. There is also clear evidence that the terrestrial component (specifically O⁺) is strongly dependent on solar cycle. Terrestrial ion source, transport, and acceleration regions have been identified in the polar auroral region, over the polar caps, in the magnetospheric boundary layers, and within the magnetotail lobes and plasma sheet boundary layer. Combining our present knowledge of these various magnetospheric ion populations, it is concluded that the primary terrestrial ion circulation pattern associated with enhanced geomagnetic activity involves direct injection from the auroral ion acceleration region into the plasma sheet boundary layer and central plasma sheet. The observed terrestrial component of the magnetospheric boundary layer and magnetotail lobes are inadequate to provide the required influx. They may, however, contribute significantly to the maintenence of the plasma sheet terrestrial ion population, particularly during periods of reduced geomagnetic activity. It is further concluded, on the basis of the relative energy distributions of H⁺ and O⁺ in the plasma sheet, that O⁺ probably contributes significantly to the ring current population at energies inaccessible to present ion composition instrumentation (? 30 keV).     

Interaction of Titan's atmosphere with Saturn's magnetosphere

The Voyager 1 measurements made during the Titan flyby reveal that Saturn's rotating magnetospheric plasma interacts directly with Titan's neutral atmosphere and ionosphere. This results from the lack of an intrinsic magnetic field at Titan. The interaction induces a magnetosphere which deflects the flowing plasma around Titan and forms a plasma wake downstream. Within the tail of the induced magnetosphere, ions of ionospheric origin flow away from Titan. Just outside Titan's magnetosphere, a substantial ion-exosphere forms from an extensive hydrogen-nitrogen exosphere. The exospheric ions are picked up and carried downstream into the wake by the plasma flowing around Titan. Mass loading produced by the addition of exospheric ions slows the wake plasma down considerably in the vicinity of the magnetopause.     

Neoplastic cell transformation by high-LET radiation: molecular mechanisms.

Experimental data on molecular mechanisms are essential for understanding the bioeffects of radiation and for developing biophysical models, which can help in determining the shape of dose-response curves at very low doses, e.g., doses less than 1 cGy. Although it has been shown that ionizing radiation can cause neoplastic cell transformation directly, that high-LET heavy ions in general can be more effective than photons in transforming cells, and that the radiogenic cell transformation is a multi-step process [correction of processes], we know very little about the molecular nature of lesions important for cell transformation, the relationship between lethal and transformational damages, and the evolution of initial damages into final chromosomal aberrations which alter the growth control of cells. Using cultured mouse embryo cells (C3H10T1/2) as a model system, we have collected quantitative data on dose-response curves for heavy ions with various charges and energies. An analysis of these quantitative data suggested that two DNA breaks formed within 80 angstroms may cause cell transformation and that two DNA breaks formed within 20 angstroms may be lethal. Through studies with restriction enzymes which produce DNA damages at specific sites, we have found that DNA double strand breaks, including both blunt- and cohesive-ended breaks, can cause cell transformation in vitro. These results indicate that DNA double strand breaks can be important primary lesions for radiogenic cell transformation and that blunt-ended double strand breaks can form lethal as well as transformational damages due to misrepair or incomplete repair in the cell. The RBE-LET relationship is similar for HGPRT gene mutation, chromosomal deletion, and cell transformation, suggesting common lesions may be involved in these radiation effects. The high RBE of high-LET radiation for cell killing and neoplastic cell transformation is most likely related to its effectiveness in producing DNA double strand breaks in mammalian cells. At present the role of oncogenes in radiation cell transformation is unclear.     

Software intensive systems safety analysis

Two important elements in the avionics suite of modern aircraft are: the flight control system (FCS) and the flight management system (FMS). The FCS provides the capability to stabilize and control the aircraft, while the FMS is responsible for flight planning and navigation. A clear trend in the aerospace industry is to place greater reliance on software systems, and many FCS and FMS subsystems are implemented primarily in software. For example, within the FCS is the flight guidance system (FGS) that generates roll and pitch guidance commands. Similarly, within the FMS is the vertical navigation (VNAV) function that acts like a third crew member in the cockpit, ordering mode change requests and resetting target altitude values to enable the aircraft to track the vertical flight plan. We have developed formal, executable models of the requirements for the mode logic of a FGS and for portions of the VNAV functionality. We have also conducted a comprehensive software safety analysis on the FGS mode logic model, and are completing the analysis of the VNAV model. This analysis uses as its starting point several "traditional" safety analysis techniques such as a functional hazard assessment (FHA), a fault tree analysis (FTA), and a failure mode effects analysis (FMEA). However, we are also using formal methods techniques known as model checking and theorem proving to verify the presence of safety properties in the model. This paper summarizes the (now completed) safety analysis that was performed on the FGS model, and highlights the similarities and differences with the (still on-going) safety analysis of the FMS model. In particular, we summarize progress made to date in the use of formal methods to verify the presence of the required safety properties in the models themselves.     

Tissue responses to low protracted doses of high LET radiations or photons: early and late damage relevant to radio-protective countermeasures.

Early and late murine tissue responses to single or fractionated low doses of heavy charged particles, fission-spectrum neutrons or gamma rays are considered. Damage to the hematopoietic system is emphasized, but results on acute lethality, host response to challenge with transplanted leukemia cells and life-shortening are presented. Low dose rates per fraction were used in some neutron experiments. Split-dose lethality studies (LD 50/30) with fission neutrons indicated greater accumulation of injury during a 9 fraction course (over 17 days) than was the case for gamma-radiation. When total doses of 96 or 247 cGy of neutrons or gamma rays were given as a single dose or in 9 fractions, a significant sparing effect on femur CFU-S depression was observed for both radiation qualities during the first 11 days, but there was not an earlier return to normal with dose fractionation. During the 9 fraction sequence, a significant sparing effect of low dose rate on CFU-S depression was observed in both neutron and gamma-irradiated mice. CFU-S content at the end of the fractionation sequence did not correlate with measured LD 50/30. Sustained depression of femur and spleen CFU-S and a significant thrombocytopenia were observed when a total neutron dose of 240 cGy was given in 72 fractions over 24 weeks at low dose rates. The temporal aspects of CFU-S repopulation were different after a single versus fractionated neutron doses. The sustained reduction in the size of the CFU-S population was accompanied by an increase in the fraction in DNA synthesis. The proliferation characteristics and effects of age were different for radial CFU-S population closely associated with bone, compared with the axial population that can be readily aspirated from the femur. In aged irradiated animals, the CFU-S proliferation/redistribution response to typhoid vaccine showed both an age and radiation effect. After high single doses of neutrons or gamma rays, a significant age- and radiation-related deficiency in host defense mechanisms was detected by a shorter mean survival time following challenge with transplantable leukemia cells. Comparison of dose-response curves for life shortening after irradiation with fission-spectrum neutrons or high energy silicon particles indicated high initial slopes for both radiation qualities at low doses, but for higher doses of silicon, the effect per Gy decreased to a value similar to that for gamma rays. The two component life-shortening curve for silicon particles has implications for the potential efficacy of radioprotectants. Recent studies on protection against early and late effects by aminothiols, prostaglandins, and other compounds are discussed.