Lichens survive in space: results from the 2005 LICHENS experiment

This experiment was aimed at establishing, for the first time, the survival capability of lichens exposed to space conditions. In particular, the damaging effect of various wavelengths of extraterrestrial solar UV radiation was studied. The lichens used were the bipolar species Rhizocarpon geographicum and Xanthoria elegans, which were collected above 2000 m in the mountains of central Spain and as endolithic communities inhabiting granites in the Antarctic Dry Valleys. Lichens were exposed to space in the BIOPAN-5 facility of the European Space Agency; BIOPAN-5 is located on the outer shell of the Earth-orbiting FOTON-M2 Russian satellite. The lichen samples were launched from Baikonur by a Soyuz rocket on May 31, 2005, and were returned to Earth after 16 days in space, at which time they were tested for survival. Chlorophyll fluorescence was used for the measurement of photosynthetic parameters. Scanning electron microscopy in back-scattered mode, low temperature scanning electron microscopy, and transmission electron microscopy were used to study the organization and composition of both symbionts. Confocal laser scanning microscopy, in combination with the use of specific fluorescent probes, allowed for the assessment of the physiological state of the cells. All exposed lichens, regardless of the optical filters used, showed nearly the same photosynthetic activity after the flight as measured before the flight. Likewise, the multimicroscopy approach revealed no detectable ultrastructural changes in most of the algal and fungal cells of the lichen thalli, though a greater proportion of cells in the flight samples had compromised membranes, as revealed by the LIVE/DEAD BacLight Bacterial Viability Kit. These findings indicate that most lichenized fungal and algal cells can survive in space after full exposure to massive UV and cosmic radiation, conditions proven to be lethal to bacteria and other microorganisms. The lichen upper cortex seems to provide adequate protection against solar radiation. Moreover, after extreme dehydration induced by high vacuum, the lichens proved to be able to recover, in full, their metabolic activity within 24 hours.     

How rare is complex life in the Milky Way?

An integrated Earth system model was applied to calculate the number of habitable Earth-analog planets that are likely to have developed primitive (unicellular) and complex (multicellular) life in extrasolar planetary systems. The model is based on the global carbon cycle mediated by life and driven by increasing stellar luminosity and plate tectonics. We assumed that the hypothetical primitive and complex life forms differed in their temperature limits and CO(2) tolerances. Though complex life would be more vulnerable to environmental stress, its presence would amplify weathering processes on a terrestrial planet. The model allowed us to calculate the average number of Earth-analog planets that may harbor such life by using the formation rate of Earth-like planets in the Milky Way as well as the size of a habitable zone that could support primitive and complex life forms. The number of planets predicted to bear complex life was found to be approximately 2 orders of magnitude lower than the number predicted for primitive life forms. Our model predicted a maximum abundance of such planets around 1.8 Ga ago and allowed us to calculate the average distance between potentially habitable planets in the Milky Way. If the model predictions are accurate, the future missions DARWIN (up to a probability of 65%) and TPF (up to 20%) are likely to detect at least one planet with a biosphere composed of complex life.     

CCD互相关粒子图像测速及其在对抗流流场测量中的应用

在常规自相关粒子图像测速（ＰＩＶ）系统的基础上,发展了一个采用ＣＣＤ像机的互相关粒子图像测速系统。用这一新系统测量了对抗流流场的结果表明,这一技术克服了自相关粒子图像测速技术在测量具有滞止点的流场中的困难。实验说明,与自相关粒子图像测速技术相比,这一技术更快捷有效。     

布鲁诺图诺尼航空展

布鲁诺（Brno)位于捷克南部邻近奥地利的位置,距离维也纳不到150公里,这是一个典型东欧城市,市内古迹众多、风景优美,在旅游旺季经常可以看到大批西方游客驻足街头,今年夏天的布鲁诺尤其热闹,美国空军的雷鸟(Thunderblrds)特技小组在欧洲巡回表演活动中造访了这个小城市,为今年的布鲁诺图谔尼航空展(Brno—Turany Airshow)大幅增加了观赏性。     

Survival and germinability of Bacillus subtilis spores exposed to simulated Mars solar radiation: implications for life detection and planetary protection

Bacterial spores have been considered as microbial life that could survive interplanetary transport by natural impact processes or human spaceflight activity. Deposition of terrestrial microbes or their biosignature molecules onto the surface of Mars could negatively impact life detection experiments and planetary protection measures. Simulated Mars solar radiation, particularly the ultraviolet component, has been shown to reduce spore viability, but its effect on spore germination and resulting production of biosignature molecules has not been explored. We examined the survival and germinability of Bacillus subtilis spores exposed to simulated martian conditions that include solar radiation. Spores of B. subtilis that contain luciferase resulting from expression of an sspB-luxAB gene fusion were deposited on aluminum coupons to simulate deposition on spacecraft surfaces and exposed to simulated Mars atmosphere and solar radiation. The equivalent of 42 min of simulated Mars solar radiation exposure reduced spore viability by nearly 3 logs, while germination-induced bioluminescence, a measure of germination metabolism, was reduced by less than 1 log. The data indicate that spores can retain the potential to initiate germination-associated metabolic processes and produce biological signature molecules after being rendered nonviable by exposure to Mars solar radiation.     

A numerical optimization of high altitude testing facility for wind tunnel experiments

High altitude test facilities are required to test the high area ratio nozzles operating at the upper stages of rocket in the nozzle full flow conditions.It is typically achieved by creating the ambient pressure equal or less than the nozzle exit pressure.On average,air/GN2is used as active gas for ejector system that is stored in the high pressure cylinders.The wind tunnel facilities are used for conducting aerodynamic simulation experiments at/under various flow velocities and operating conditions.However,constructing both of these facilities require more laboratory space and expensive instruments.Because of this demerit,a novel scheme is implemented for conducting wind tunnel experiments by using the existing infrastructure available in the high altitude testing(HAT)facility.This article presents the details about the methods implemented for suitably modifying the sub-scale HAT facility to conduct wind tunnel experiments.Hence,the design of nozzle for required area ratio A/A*,realization of test section and the optimized configuration are focused in the present analysis.Specific insights into various rocket models including high thrust cryogenic engines and their holding mechanisms to conduct wind tunnel experiments in the HAT facility are analyzed.A detailed CFD analysis is done to propose this conversion without affecting the existing functional requirements of the HAT facility.     

拉压、扭转载荷下铸铝合金的亿周疲劳性能研究

应用超声疲劳试验机对铸铝合金2-AS5U3G-Y35在扭转和拉压循环载荷下进行了超高周疲劳性能测试.介绍了超声扭转疲劳试验装置的设计.应用35 Hz常规疲劳试验机和20 kHz的超声疲劳试验机完成应力比R=-1的拉压、扭转疲劳试验,进而研究不同载荷条件、加载频率对铸铝合金超高周疲劳性能的影响.S-N曲线显示,铝合金在105～1010疲劳周次间仍发生疲劳断裂,不存在疲劳极限.断口分析表明,在超高周循环拉压载荷下,疲劳裂纹常萌生于试样次表面材料内部缩孔.与循环拉压载荷下的疲劳断裂机理不同,在循环扭转载荷下疲劳裂纹主要萌生于试样表面,疲劳断裂面为一种典型的沿试样轴向45°的螺旋面,即沿最大主应力平面断裂.扭转疲劳断面清晰的剪切条带表明扭转疲劳断裂实质上是剪切断裂.     

Sulfur-oxidizing chemolithotrophic proteobacteria dominate the microbiota in high arctic thermal springs on Svalbard

The thermal springs Trollosen and Fisosen, located on the High Arctic archipelago Svalbard, discharge saline groundwaters rich in hydrogen sulfide and ammonium through a thick layer of permafrost. Large amounts of biomass that consist of filamentous microorganisms containing sulfur granules, as analyzed with energy dispersive X-ray analysis, were found in the outflow. Prokaryotic 16S rRNA gene libraries and quantitative polymerase chain reaction (qPCR) analyses reported bacteria of the γ- and ?-proteobacterial classes as the dominant organisms in the filaments and the planktonic fractions, closely related to known chemolithoautotrophic sulfur oxidizers (Thiotrix and Sulfurovum). Archaea comprised ～1% of the microbial community, with the majority of sequences affiliated with the Thaumarchaeota. Archaeal and bacterial genes coding for a subunit of the enzyme ammonia monooxygenase (amoA) were detected, as well as 16S?rRNA genes of Nitrospira, all of which is indicative of potential complete nitrification in both springs. 16S rRNA sequences related to methanogens and methanotrophs were detected as well. This study provides evidence that the microbial communities in Trollosen and Fisosen are sustained by chemolithotrophy, mainly through the oxidation of reduced sulfur compounds, and that ammonium and methane might be minor, additional sources of energy and carbon.     

The influence of microgravity on invasive growth in Saccharomyces cerevisiae

This study investigates the effects of microgravity on colony growth and the morphological transition from single cells to short invasive filaments in the model eukaryotic organism Saccharomyces cerevisiae. Two-dimensional spreading of the yeast colonies grown on semi-solid agar medium was reduced under microgravity in the Σ1278b laboratory strain but not in the CMBSESA1 industrial strain. This was supported by the Σ1278b proteome map under microgravity conditions, which revealed upregulation of proteins linked to anaerobic conditions. The Σ1278b strain showed a reduced invasive growth in the center of the yeast colony. Bud scar distribution was slightly affected, with a switch toward more random budding. Together, microgravity conditions disturb spatially programmed budding patterns and generate strain-dependent growth differences in yeast colonies on semi-solid medium.     

SOLID3: a multiplex antibody microarray-based optical sensor instrument for in situ life detection in planetary exploration

The search for unequivocal signs of life on other planetary bodies is one of the major challenges for astrobiology. The failure to detect organic molecules on the surface of Mars by measuring volatile compounds after sample heating, together with the new knowledge of martian soil chemistry, has prompted the astrobiological community to develop new methods and technologies. Based on protein microarray technology, we have designed and built a series of instruments called SOLID (for "Signs Of LIfe Detector") for automatic in situ detection and identification of substances or analytes from liquid and solid samples (soil, sediments, or powder). Here, we present the SOLID3 instrument, which is able to perform both sandwich and competitive immunoassays and consists of two separate functional units: a Sample Preparation Unit (SPU) for 10 different extractions by ultrasonication and a Sample Analysis Unit (SAU) for fluorescent immunoassays. The SAU consists of five different flow cells, with an antibody microarray in each one (2000 spots). It is also equipped with an exclusive optical package and a charge-coupled device (CCD) for fluorescent detection. We demonstrated the performance of SOLID3 in the detection of a broad range of molecular-sized compounds, which range from peptides and proteins to whole cells and spores, with sensitivities at 1-2?ppb (ng?mL?1) for biomolecules and 10? to 103 spores per milliliter. We report its application in the detection of acidophilic microorganisms in the Río Tinto Mars analogue and report the absence of substantial negative effects on the immunoassay in the presence of 50?mM perchlorate (20 times higher than that found at the Phoenix landing site). Our SOLID instrument concept is an excellent option with which to detect biomolecules because it avoids the high-temperature treatments that may destroy organic matter in the presence of martian oxidants.