Influence of microgravity on mitogen binding and cytoskeleton in Jurkat cells.

The effects of microgravity on Jurkat cells--a T-lymphoid cell line--was studied on a sounding rocket flight. An automated pre-programmed instrument permitted the injection of fluorescent labelled concanavalin A (Con A), culture medium and/or fixative at given times. An in-flight 1 g centrifuge allowed the comparison of the data obtained in microgravity with a 1 g control having the same history related to launch and re-entry. After flight, the cells fixed either at the onset of microgravity or after a or 12 minute incubation time with fluorescent concanavalin A were labelled for vimentin and actin and analysed by fluorescence microscopy. Binding of Con A to Jurkat cells is not influenced by microgravity, whereas patching of the Con A receptors is significantly lower. A significant higher number of cells show changes in the structure of vimentin in microgravity. Most evident is the appearance of large bundles, significantly increased in the microgravity samples. No changes are found in the structure of actin and in the colocalisation of actin on the inner side of the cell membrane with the Con A receptors after binding of the mitogen.     

Enhancing Maintainability of the AWG-10 Through Built-In-Test Equipment

Multimode airborne weapons systems are now a reality. Because of the increased complexity of these systems, excessive maintenance time has become a problem. This paper describes an approach to reducing maintenance time through the use of semiautomatic built-in-test equipment within the framework of an integrated maintenance program. Emphasis is placed on the methods and hardware involved in the built-in-test mechanization as well as describing the development history of this type of maintenance concept.     

Design of Zigzag FM Signals

Since no practical method is available for synthesizing radar waveforms, a sizable effort has been directed into studies of the matched-filter response, or ambiguity function, of many waveforms. In this paper, we investigate the class of FM signals whose instantaneous frequency varies in a zigzag pattern. The waveforms thus consist of linear FM segments and are relatively easy to generate and process. The paper discusses the relation between the characteristics of the waveform and the features of the associated ambiguity function. The effects studied include those of signal repetition, changes in the FM slope, phase-shift and frequency-shift coding, and staggering of frequency step and segment duration. Ambiguity functions of interesting waveforms illustrate the general results. These ambiguity functions are computer-plotted projections of the three-dimensional surface above the delay-Doppler plane.     

Structural health management technologies for inflatable/deployable structures: Integrating sensing and self-healing

Inflatable/deployable structures are under consideration as habitats for future Lunar surface science operations. The use of non-traditional structural materials combined with the need to maintain a safe working environment for extended periods in a harsh environment has led to the consideration of an integrated structural health management system for future habitats, to ensure their integrity. This article describes recent efforts to develop prototype sensing technologies and new self-healing materials that address the unique requirements of habitats comprised mainly of soft goods. A new approach to detecting impact damage is discussed, using addressable flexible capacitive sensing elements and thin film electronics in a matrixed array. Also, the use of passive wireless sensor tags for distributed sensing is discussed, wherein the need for on-board power through batteries or hardwired interconnects is eliminated. Finally, the development of a novel, microencapuslated self-healing elastomer with applications for inflatable/deployable habitats is reviewed.     

Analysis of nonlinear deformation and stability of reinforced elliptic cylindrical shells under bending with internal pressure

A finite element statement of solving problems on stability of reinforced elliptic cylindrical shells taking into account momentness and nonlinearity of their subcritical stress strain state is presented. The explicit expressions for displacements of noncircular cylindrical shell elements as rigid bodies are determined by integrating the equations obtained by equating the components of linear strains to zero. These expressions were used to construct the form functions for an efficient quadrilateral finite element of natural curvature. An efficient numerical algorithm of nonlinear deformation and stability of shells was developed. The stability of reinforced elliptic cylindrical shells under combined loading by bending moment, transverse boundary force and internal pressure is analyzed. We also examine how the critical loads are affected by the strain nonlinearity and ellipticity of shell deformation at the subcritical stage.     

Lithium Depletion in the Sun: A Study of Mixing Based on Hydrodynamical Simulations

Based on radiation hydrodynamics modeling of stellar convection zones, a diffusion scheme has been devised describing the downward penetration of convective motions beyond the Schwarzschild boundary (overshoot) into the radiative interior. This scheme of exponential diffusive overshoot has already been successfully applied to AGB stars. Here we present an application to the Sun in order to determine the time scale and depth extent of this additional mixing, i.e. diffusive overshoot at the base of the convective envelope. We calculated the associated destruction of lithium during the evolution towards and on the main-sequence. We found that the slow-mixing processes induced by the diffusive overshoot may lead to a substantial depletion of lithium during the Sun's main-sequence evolution. This revised version was published online in June 2006 with corrections to the Cover Date.     

Considerations for the Short-Term Stability of Frequency Multipliers

The effects of frequency multiplication circuits on the short-term stability of an oscillator system are analyzed. The short-term stability of real components such as varactor multipliers are compared to the short-term stability of an ?ideal? frequency multiplier which maintains the stability of the basic oscillator. In addition, it is shown that positive feedback like that used in a synchronized oscillator, while useful for tracking, degrades short-term stability.     

High temperature alters the growth reaction of Pottia protonemata.

Under gravistimulation, dark-grown protonemata of Pottia intermedia revealed negative gravitropism with a growth rate of approximately 28 μm·h⁻¹ at room temperature (20 °C). In 7 days, the protonema formed a bundle of vertically oriented filaments. At an elevated temperature (30 °C), bundles of vertically growing filaments were also formed. However, both filament growth rate and amplitude of the gravicurvature were reduced. Red light (RL) irradiation induced a positive phototropism of most apical protonemal cells at 20 °C. In a following period of darkness, approximately two-thirds of such cells began to grow upward again, recovering their negative gravitropism. RL irradiation at the elevated temperature caused a partial increase in the number of protonemal cells with negative phototropism, but the protonemata did not exhibit negative gravitropism after transfer to darkness. The negative gravitropic reaction was renewed only when protonemata were placed at 20 °C. A dramatic decrease in starch amount in protonemal apical cells, which are sensitive to both gravity and light, occurred at the higher temperature. Such a decrease may be one of the reasons for the inhibition of the protonemal gravireaction at the higher temperature. The observation has a bearing on the starch-statolith theory.     

Sampled-Data Control System Analysis Using the Poisson Summation Rule

A technique is presented which simplifies the analysis of sampled-data control systems. The use of a finite-term approximation to the Poisson summation rule, in conjunction with digital computer evaluation, is presented as a good alternative to use of the Z transform whenever frequency-domain analysis is required. The Poisson summation rule is reviewed and its application to sampled-data system analysis is discussed. The form of the computer program used in the analysis is presented, along with a design criterion to evaluate the accuracy of the technique. A detailed example of a typical application is presented.