Efficacy of an ototoxic aminoglycoside (gentamicin) on the differentiation of the inner ear of cichlid fish.

Previous investigations revealed that the growth of fish inner ear otoliths depends on the amplitude and the direction of gravity, thus suggesting the existence of a (negative) feedback mechanism. In the course of these experiments, it was shown that altered gravity both affected otolith size (and thus the provision of the proteinacious matrix) as well as the incorporation of calcium. It is hitherto unknown, as of whether sensory hair cells are involved either in the regulation of otolith growth or in the provision of otolithic material (such as protein or inorganic components) or even both. The ototoxic aminoglycoside gentamicin (GM) damages hair cells in many vertebrates (and is therefore used for the treatment of Meniere's disease in humans). The present study was thus designed to determine as of whether vestibular sensory cells are needed for otolith growth by applying GM in order to induce a (functionally relevant) loss of these cells. Developing cichlid fish Oreochromis mossambicus were therefore immersed in 120 mg/l GM for 10 or 21 days. At the beginning and at the end of the experimental periods, the fish were incubated in the calcium-tracer alizarin complexone (AC). After the experiment, otoliths were dissected and the area grown during GM-exposure (i.e., the area enclosed by the two AC labellings) was determined planimetrically. The results showed that incubating the animals in a GM-solution had no effect on otolith growth, but the development of otolith asymmetry was affected. Ultrastructural examinations of the sensory hair cells revealed that they had obviously not been affected by GM-treatment (no degenerative morphological features observed). Overall, the present results suggest that hair cells are not affected by GM concerning their possible role in (general) otolith growth, but that these cells indeed might have transitionally been impaired by GM resulting in a decreased capacity of regulating otolith symmetry.     

Atmospheric Science with InSight

In November 2018, for the first time a dedicated geophysical station, the InSight lander, will be deployed on the surface of Mars. Along with the two main geophysical packages, the Seismic Experiment for Interior Structure (SEIS) and the Heat-Flow and Physical Properties Package (HP³), the InSight lander holds a highly sensitive pressure sensor (PS) and the Temperature and Winds for InSight (TWINS) instrument, both of which (along with the InSight FluxGate (IFG) Magnetometer) form the Auxiliary Sensor Payload Suite (APSS). Associated with the RADiometer (RAD) instrument which will measure the surface brightness temperature, and the Instrument Deployment Camera (IDC) which will be used to quantify atmospheric opacity, this will make InSight capable to act as a meteorological station at the surface of Mars. While probing the internal structure of Mars is the primary scientific goal of the mission, atmospheric science remains a key science objective for InSight. InSight has the potential to provide a more continuous and higher-frequency record of pressure, air temperature and winds at the surface of Mars than previous in situ missions. In the paper, key results from multiscale meteorological modeling, from Global Climate Models to Large-Eddy Simulations, are described as a reference for future studies based on the InSight measurements during operations. We summarize the capabilities of InSight for atmospheric observations, from profiling during Entry, Descent and Landing to surface measurements (pressure, temperature, winds, angular momentum), and the plans for how InSight’s sensors will be used during operations, as well as possible synergies with orbital observations. In a dedicated section, we describe the seismic impact of atmospheric phenomena (from the point of view of both “noise” to be decorrelated from the seismic signal and “signal” to provide information on atmospheric processes). We discuss in this framework Planetary Boundary Layer turbulence, with a focus on convective vortices and dust devils, gravity waves (with idealized modeling), and large-scale circulations. Our paper also presents possible new, exploratory, studies with the InSight instrumentation: surface layer scaling and exploration of the Monin-Obukhov model, aeolian surface changes and saltation / lifing studies, and monitoring of secular pressure changes. The InSight mission will be instrumental in broadening the knowledge of the Martian atmosphere, with a unique set of measurements from the surface of Mars.     

Studies on precellular evolution: the encapsulation of polyribonucleotides by liposomes.

Liposomes are 5 to 50 micron vesicles with an internal aqueous environment, whose amphiphilic lipidic components self-assemble into systems with at least one double-layered membrane. Liposomes have been suggested as possible models of precellular systems formed in the early Archean Earth from lipids of non-enzymatic origin. Since it is generally accepted that RNA molecules preceded double-stranded DNA molecules as genetic material, we have studied the encapsulation of polyribonucleotides within liposomes made from dipalmitoyl phosphatidylcholine, and from egg yolk phosphatidylcholine to which cholesterol was added in some cases. The liposomes were prepared under anoxic conditions following the reverse phase evaporation method described by Szoka and Papahadjopoulos. Quantitative determinations show that approximately 50% of the available lipids form liposomes, and that up to 5% of the polyribonucleotides can be entrapped by them. We have also studied the encapsulation of polyribonucleotides in the presence of 1) urea and cyanamide, two non-electrolytes that have been used as prebiotic condensing agents, and 2) of Zn++ and Pb++, two cations employed in the non-enzymatic template-directed synthesis of polyribonucleotides from activated nucleotides.     

Perception of gravity by plants.

Physical principles can be used to predict some features about the gravity perception system in plants. The nature of the system has made it rather elusive, so this approach represents an additional source of information to help find it. For a gravitational stimulus to be detected, two masses must move relative to each other in a manner which causes a significant amount of work to be done on a receptor. Relative to cellular dimensions, the masses must be large, be dense and move noticeable distances. The main sources of noise are thermal motion and flexing of the plant tissue. Some new models for the function of amyloplasts as statoliths are presented.     

The Search-Coil Magnetometer for MMS

The tri-axial search-coil magnetometer (SCM) belongs to the FIELDS instrumentation suite on the Magnetospheric Multiscale (MMS) mission (Torbert et al. in Space Sci. Rev. (2014), this issue). It provides the three magnetic components of the waves from 1 Hz to 6 kHz in particular in the key regions of the Earth’s magnetosphere namely the subsolar region and the magnetotail. Magnetospheric plasmas being collisionless, such a measurement is crucial as the electromagnetic waves are thought to provide a way to ensure the conversion from magnetic to thermal and kinetic energies allowing local or global reconfigurations of the Earth’s magnetic field. The analog waveforms provided by the SCM are digitized and processed inside the digital signal processor (DSP), within the Central Electronics Box (CEB), together with the electric field data provided by the spin-plane double probe (SDP) and the axial double probe (ADP). On-board calibration signal provided by DSP allows the verification of the SCM transfer function once per orbit. Magnetic waveforms and on-board spectra computed by DSP are available at different time resolution depending on the selected mode. The SCM design is described in details as well as the different steps of the ground and in-flight calibrations.     

Synaptic plasticity and gravity: ultrastructural, biochemical and physico-chemical fundamentals.

On the basis of quantitative disturbances of the swimming behaviour of aquatic vertebrates ("loop-swimming" in fish and frog larvae) following long-term hyper-g-exposure the question was raised whether or not and to what extent changes in the gravitational vector might influence the CNS at the cellular level. Therefore, by means of histological, histochemical and biochemical analyses the effect of 2-4 x g for 9 days on the gross morphology of the fish brain, and on different neuronal enzymes was investigated. In order to enable a more precise analysis in future-microgravity-experiments of any gravity-related effects on the neuronal synapses within the gravity-perceptive integration centers differentiated electron-microscopical and electronspectroscopical techniques have been developed to accomplish an ultrastructural localization of calcium, a high-affinity Ca2(+)-ATPase, creatine kinase and cytochrome oxidase. In hyper-g animals vs. 1-g controls, a reduction of total brain volume (15%), a decrease in creatine kinase activity (20%), a local increase in cytochrome oxidase activity, but no differences in Ca2+/Mg(2+)-ATPase activities were observed. Ultrastructural peculiarities of synaptic contact formation in gravity-related integration centers (Nucleus magnocellularis) were found. These results are discussed on the basis of a direct effect of hyper-gravity not only on the gravity-sensitive neuronal integration centers but possibly also on the physico-chemical properties of the lipid bilayer of neuronal membranes in general.     

Simulations of absorbed dose on the phantom surface of MATROSHKA-R experiment at the ISS

The health risks associated with exposure to various components of space radiation are of great concern when planning manned long-term interplanetary missions, such as future missions to Mars. Since it is not possible to measure the radiation environment inside of human organs in deep space, simulations based on radiation transport/interaction codes coupled to phantoms of tissue equivalent materials are used. However, the calculated results depend on the models used in the codes, and it is therefore necessary to verify their validity by comparison with measured data. The goal of this paper is to compare absorbed doses obtained in the MATROSHKA-R experiment performed at the International Space Station (ISS) with simulations performed with the three-dimensional Monte Carlo Particle and Heavy-Ion Transport code System (PHITS). The absorbed dose was measured using passive detectors (packages of thermoluminescent and plastic nuclear track detectors) placed on the surface of the spherical tissue equivalent phantom MATROSHKA-R, which was exposed aboard the ISS in the Service Zvezda Module from December 2005 to September 2006. The data calculated by PHITS assuming an ISS shielding of 3 g/cm² and 5 g/cm² aluminum mass thickness were in good agreement with the measurements. Using a simplified geometrical model of the ISS, the influence of variations in altitude and wall mass thickness of the ISS on the calculated absorbed dose was estimated. The uncertainties of the calculated data are also discussed; the relative expanded uncertainty of absorbed dose in phantom was estimated to be 44% at a 95% confidence level.     

Properties of Ground Level Enhancement Events and the Associated Solar Eruptions During Solar Cycle 23

Solar cycle 23 witnessed the most complete set of observations of coronal mass ejections (CMEs) associated with the Ground Level Enhancement (GLE) events. We present an overview of the observed properties of the GLEs and those of the two associated phenomena, viz., flares and CMEs, both being potential sources of particle acceleration. Although we do not find a striking correlation between the GLE intensity and the parameters of flares and CMEs, the solar eruptions are very intense involving X-class flares and extreme CME speeds (average ～2000?km/s). An M7.1 flare and a 1200?km/s CME are the weakest events in the list of 16 GLE events. Most (80?%) of the CMEs are full halos with the three non-halos having widths in the range 167 to 212?degrees. The active regions in which the GLE events originate are generally large: 1290?msh (median 1010?msh) compared to 934?msh (median: 790?msh) for SEP-producing active regions. For accurate estimation of the CME height at the time of metric type?II onset and GLE particle release, we estimated the initial acceleration of the CMEs using flare and CME observations. The initial acceleration of GLE-associated CMEs is much larger (by a factor of 2) than that of ordinary CMEs (2.3?km/s² vs. 1?km/s²). We confirmed the initial acceleration for two events for which CME measurements are available in the inner corona. The GLE particle release is delayed with respect to the onset of all electromagnetic signatures of the eruptions: type?II bursts, low frequency type?III bursts, soft X-ray flares and CMEs. The presence of metric type?II radio bursts some 17?min (median: 16?min; range: 3 to 48?min) before the GLE onset indicates shock formation well before the particle release. The release of GLE particles occurs when the CMEs reach an average height of ～3.09?R _s (median: 3.18?R _s ; range: 1.71 to 4.01?R _s ) for well-connected events (source longitude in the range W20–W90). For poorly connected events, the average CME height at GLE particle release is ～66?% larger (mean: 5.18?R _s ; median: 4.61?R _s ; range: 2.75–8.49?R _s ). The longitudinal dependence is consistent with shock accelerations because the shocks from poorly connected events need to expand more to cross the field lines connecting to an Earth observer. On the other hand, the CME height at metric type?II burst onset has no longitudinal dependence because electromagnetic signals do not require magnetic connectivity to the observer. For several events, the GLE particle release is very close to the time of first appearance of the CME in the coronagraphic field of view, so we independently confirmed the CME height at particle release. The CME height at metric type?II burst onset is in the narrow range 1.29 to 1.8?R _s , with mean and median values of 1.53 and 1.47?R _s . The CME heights at metric type?II burst onset and GLE particle release correspond to the minimum and maximum in the Alfvén speed profile. The increase in CME speed between these two heights suggests an increase in Alfvénic Mach number from?2 to?3. The CME heights at GLE particle release are in good agreement with those obtained from the velocity dispersion analysis (Reames in Astrophys. J. 693:812, 2009a; Astrophys. J. 706:844, 2009b) including the source longitude dependence. We also discuss the implications of the delay of GLE particle release with respect to complex type?III bursts by ～18?min (median: 16?in; range: 2 to 44?min) for the flare acceleration mechanism. A?similar analysis is also performed on the delay of particle release relative to the hard X-ray emission.     

The Radiation Assessment Detector (RAD) Investigation

The Radiation Assessment Detector (RAD) on the Mars Science Laboratory (MSL) is an energetic particle detector designed to measure a broad spectrum of energetic particle radiation. It will make the first-ever direct radiation measurements on the surface of Mars, detecting galactic cosmic rays, solar energetic particles, secondary neutrons, and other secondary particles created both in the atmosphere and in the Martian regolith. The radiation environment on Mars, both past and present, may have implications for habitability and the ability to sustain life. Radiation exposure is also a major concern for future human missions. The RAD instrument combines charged- and neutral-particle detection capability over a wide dynamic range in a compact, low-mass, low-power instrument. These capabilities are required in order to measure all the important components of the radiation environment. RAD consists of the RAD Sensor Head (RSH) and the RAD Electronics Box (REB) integrated together in a small, compact volume. The RSH contains a solid-state detector telescope with three silicon PIN diodes for charged particle detection, a thallium doped Cesium Iodide scintillator, plastic scintillators for neutron detection and anti-coincidence shielding, and the front-end electronics. The REB contains three circuit boards, one with a novel mixed-signal ASIC for processing analog signals and an associated control FPGA, another with a second FPGA to communicate with the rover and perform onboard analysis of science data, and a third board with power supplies and power cycling or “sleep”-control electronics. The latter enables autonomous operation, independent of commands from the rover. RAD is a highly capable and highly configurable instrument that paves the way for future compact energetic particle detectors in space.     

The Mars Science Laboratory Organic Check Material

Mars Science Laboratory’s Curiosity rover carries a set of five external verification standards in hermetically sealed containers that can be sampled as would be a Martian rock, by drilling and then portioning into the solid sample inlet of the Sample Analysis at Mars (SAM) suite. Each organic check material (OCM) canister contains a porous ceramic solid, which has been doped with a fluorinated hydrocarbon marker that can be detected by SAM. The purpose of the OCM is to serve as a verification tool for the organic cleanliness of those parts of the sample chain that cannot be cleaned other than by dilution, i.e., repeated sampling of Martian rock. SAM possesses internal calibrants for verification of both its performance and its internal cleanliness, and the OCM is not used for that purpose. Each OCM unit is designed for one use only, and the choice to do so will be made by the project science group (PSG).