Development and research program for a soil-based bioregenerative agriculture system to feed a four person crew at a Mars base.

For humans to survive during long-term missions on the Martian surface, bioregenerative life support systems including food production will decrease requirements for launch of Earth supplies, and increase mission safety. It is proposed that the development of "modular biospheres"--closed system units that can be air-locked together and which contain soil-based bioregenerative agriculture, horticulture, with a wetland wastewater treatment system is an approach for Mars habitation scenarios. Based on previous work done in long-term life support at Biosphere 2 and other closed ecological systems, this consortium proposes a research and development program called Mars On Earth(TM) which will simulate a life support system designed for a four person crew. The structure will consist of 6 x 110 square meter modular agricultural units designed to produce a nutritionally adequate diet for 4 people, recycling all air, water and waste, while utilizing a soil created by the organic enrichment and modification of Mars simulant soils. Further research needs are discussed, such as determining optimal light levels for growth of the necessary range of crops, energy trade-offs for agriculture (e.g. light intensity vs. required area), capabilities of Martian soils and their need for enrichment and elimination of oxides, strategies for use of human waste products, and maintaining atmospheric balance between people, plants and soils.     

Induced abnormality in Mir- and Earth grown Super Dwarf wheat.

Super-dwarf wheat grown on the Mir space station using the Svet "Greenhouse" exhibited morphological, metabolic and reproductive abnormalities compared with Earth-grown wheat. Of prominent importance were the abnormalities associated with reproductive ontogeny and the total absence of seed formation on Mir. Changes in the apical meristem associated with transition from the vegetative phase to floral initiation and development of the reproductive spike were all typical of 'Super-Dwarf' wheat up to the point of anthesis. Observation of ruptured anthers from the Mir-grown plants revealed what appeared to be normally developed pollen. These pollen gains, however, contained only one nuclei, while normal viable pollen is tri-nucleate. A potentially important difference in the flight experiment, compared with ground reference studies, was the presence of a high level of atmospheric ethylene (1,200 ppb). Ground studies conducted by exposing 'Super-Dwarf' wheat to ethylene just prior to anthesis resulted in manifestation of the same abnormalities observed in the space flight samples.     

Induction and repair of DNA strand breaks in bovine lens epithelial cells after high LET irradiation.

The lens epithelium is the initiation site for the development of radiation induced cataracts. Radiation in the cortex and nucleus interacts with proteins, while in the epithelium, experimental results reveal mutagenic and cytotoxic effects. It is suggested that incorrectly repaired DNA damage may be lethal in terms of cellular reproduction and also may initiate the development of mutations or transformations in surviving cells. The occurrence of such genetically modified cells may lead to lens opacification. For a quantitative risk estimation for astronauts and space travelers it is necessary to know the relative biological effectiveness (RBE), because the spacial and temporal distribution of initial physical damage induced by cosmic radiation differ significantly from that of X-rays. RBEs for the induction of DNA strand breaks and the efficiency of repair of these breaks were measured in cultured diploid bovine lens epithelial cells exposed to different LET irradiation to either 300 kV X-rays or to heavy ions at the UNILAC accelerator at GSI. Accelerated ions from Z=8 (O) to Z=92 (U) were used. Strand breaks were measured by hydroxyapatite chromatography of alkaline unwound DNA (overall strand breaks). Results showed that DNA damage occurs as a function of dose, of kinetic energy and of LET. For particles having the same LET the severity of the DNA damage increases with dose. For a given particle dose, as the LET rises, the numbers of DNA strand breaks increase to a maximum and then reach a plateau or decrease. Repair kinetics depend on the fluence (irradiation dose). At any LET value, repair is much slower after heavy ion exposure than after X-irradiation. For ions with an LET of less than 10,000 keV micrometers-1 more than 90 percent of the strand breaks induced are repaired within 24 hours. At higher particle fluences, especially for low energetic particles with a very high local density of energy deposition within the particle track, a higher proportion of non-rejoined breaks is found, even after prolonged periods of incubation. At the highest LET value (16,300 keV micrometers-1) no significant repair is observed. These LET-dependencies are consistent with the current mechanistic model for radiation induced cataractogenesis which postulates that genomic damage to the surviving fraction of epithelial cells is responsible for lens opacification.     

First estimations of the aerosol optical thickness using Langley Method at Southern Brazil (29.4°S, 53.8°W)

The first estimations of the aerosol optical thickness (AOT) using Langley Method at Southern Space Observatory (SSO) at Southern Brazil (29.4°S, 53.8°W) are presented. In addition to ozone and sulphur dioxide columns, AOT can be obtained using Brewer Spectrophotometer at specific wavelengths: 306.3, 310.1, 313.5, 316.8 and 320.1 nm. The AOT was obtained for the period from November/2002 to May/2003. Very low AOT averages were obtained, whose values were about 0.21 ± 0.03 at 306.3 nm, 0.21 ± 0.02 at 310.1 nm, 0.19 ± 0.02 at 313.5 nm, 0.20 ± 0.02 at 316.8 nm and 0.20 ± 0.02 at 320.0 nm for all period analysed. Different behaviour of AOT were obtained at two daily specific periods of aerosol accumulation, one in the afternoons from November/2002 to February/2003, caused mainly by a mild biomass burning season’s in the region and other in the mornings from March to May/2003, due the high relative humidity presented in the region studied.     

The Scanning Sky Monitor (SSM) on ASTROSAT

The Scanning Sky Monitor is one of the experiments onboard the ASTROSAT, an Indian multiwavelength astronomy satellite mission. This experiment will detect and monitor X-ray transients in the energy band 2–10 keV. It is similar in design to the ASM on RXTE. It consists of position-sensitive proportional counters with one-dimensional mask. We describe the configuration of the experiment. We also discuss some of the results obtained using a detector which has already been fabricated and tested in our laboratory.     

Magnetar descendants in HMXBs?

We investigate the relation between the pulse and orbital periods in high-mass X-ray binaries (HMXBs), in order to find the candidates of magnetar descendants in HMXBs. We suggest that magnetar descendants can be found among the HMXBs with relatively shorter orbital periods and longer pulse periods.     

Orbital evolution studies of two HMXB pulsars

High mass X-ray binary (HMXB) pulsars are of two types, persistent and transient. 4U1538−52 is a persistent HMXB whose orbit was previously measured to be circular but the RXTE observations revealed an eccentric orbit. We observed this system with RXTE-PCA in August 2003 and our timing analysis supports the eccentric orbit of the system. However, we do not find any evidence for orbital evolution.

Rotational and tidal interactions between the stars of a closed binary system result in apsidal motion which can be measured in systems with eccentric orbit. 4U0115+63 is a Be-transient HMXB whose eccentric orbit was well-determined during its 1978 outburst. We report preliminary results from analysis of data obtained during the 1999 outburst of this source with the RXTE-PCA.     

Multiband echo tomography of Sco X-1

We present preliminary results of a simultaneous X-ray/optical campaign of the prototypical LMXB Sco X-1 at 1–10 Hz time resolution. Lightcurves of the high excitation Bowen/HeII emission lines and a red continuum at λ_c  6000 Å were obtained through narrow interference filters with ULTRACAM, and these were cross-correlated with simultaneous RXTE X-ray lightcurves. We find evidence for correlated variability, in particular when Sco X-1 enters the Flaring branch. The Bowen/HeII lightcurves lag the X-ray lightcurves with a light travel time which is consistent with reprocessing in the companion star while the continuum lightcurves have shorter delays consistent with reprocessing in the accretion disc.     

FEM code simulation of the magnetospheric proton fluxes

We report a study of the numeric solution to the diffusive transport equation for energetic protons magnetically trapped in the Earth's equatorial magnetosphere. The analysis takes into account the pertinent physical processes in this region, including deceleration of protons by Coulomb collisional interactions with free and bound electrons, the charge exchange process, cosmic ray albedo neutron decay source, and electric and magnetic radial diffusion. These results were obtained using the Finite Element Method with magnetic moment and geomagnetic L-shell as free variables. Steady state boundary conditions were imposed at L=1 as zero distribution function and at L=7 with proton distribution function extracted from ATS 6 satellite observations. The FEM-code yields unidirectional proton flux in the energy range of 0.1–1000 MeV at the equatorial top of the geomagnetic lines, and the results are found to be in satisfactorily agreement with the empirical NASA AP-8 model proton flux within the energy range of 0.5–100 MeV. Below 500 keV, the empirical AP-8 model proton fluxes are several orders of magnitude greater than those computed with the FEM-code at L<3. This discrepancy is difficult to explain by uncertainties of boundary spectrum parameters or transport coefficients.     

Life sciences flight hardware development for the International Space Station.

During the construction phase of the International Space Station (ISS), early flight opportunities have been identified (including designated Utilization Flights, UF) on which early science experiments may be performed. The focus of NASA's and other agencies' biological studies on the early flight opportunities is cell and molecular biology; with UF-1 scheduled to fly in fall 2001, followed by flights 8A and UF-3. Specific hardware is being developed to verify design concepts, e.g., the Avian Development Facility for incubation of small eggs and the Biomass Production System for plant cultivation. Other hardware concepts will utilize those early research opportunities onboard the ISS, e.g., an Incubator for sample cultivation, the European Modular Cultivation System for research with small plant systems, an Insect Habitat for support of insect species. Following the first Utilization Flights, additional equipment will be transported to the ISS to expand research opportunities and capabilities, e.g., a Cell Culture Unit, the Advanced Animal Habitat for rodents, an Aquatic Facility to support small fish and aquatic specimens, a Plant Research Unit for plant cultivation, and a specialized Egg Incubator for developmental biology studies. Host systems (Figure 1A, B: see text), e.g., a 2.5 m Centrifuge Rotor (g-levels from 0.01-g to 2-g) for direct comparisons between g and selectable g levels, the Life Sciences Glovebox for contained manipulations, and Habitat Holding Racks (Figure 1B: see text) will provide electrical power, communication links, and cooling to the habitats. Habitats will provide food, water, light, air and waste management as well as humidity and temperature control for a variety of research organisms. Operators on Earth and the crew on the ISS will be able to send commands to the laboratory equipment to monitor and control the environmental and experimental parameters inside specific habitats. Common laboratory equipment such as microscopes, cryo freezers, radiation dosimeters, and mass measurement devices are also currently in design stages by NASA and the ISS international partners.