DNA fragmentation by charged particle tracks.

High-LET (linear energy transfer) charged particles induce DNA double-strand breaks (DSB) in a non-random fashion in mammalian cells. The clustering of DSB, probably determined by track structure as well as chromatin conformation, results in an excess of small- and intermediate-sized DNA fragments. DNA fragmentation in normal human fibroblasts (GM5758) was analyzed by pulsed-field gel electrophoresis after irradiation with photons (60Co) or 125 keV/micrometers nitrogen ions. Compared to conventional DSB analysis, i.e. assays only measuring the fraction of DNA smaller than a single threshold, the relative biological effectiveness (RBE) for DSB induction increased with 100%. Further, the size distribution of DNA fragments showed a significant dependence on radiation quality, with an excess of fragments up to 1 Mbp. Irradiation of naked genomic DNA without histone proteins increased the DSB yields 25 and 13 times for photons and nitrogen ions, respectively. The results suggest possible roles of both track structure and chromatin organization in the distribution of DNA double-strand breaks along the chromosome.     

Issues of exploration: human health and wellbeing during a mission to Mars.

Today, the tools are in our hands to enable us to travel away from our home planet and become citizens of the solar system. Even now, we are seriously beginning to develop the robust infrastructure that will make the 21st century the Century of Space Travel. But this bold step must be taken with due concern for the health, safety and wellbeing of future space explorers. Our long experience with space biomedical research convinces us that, if we are to deal effectively with the medical and biomedical issues of exploration, then dramatic and bold steps are also necessary in this field. We can no longer treat the human body as if it were composed of muscles, bones, heart and brain acting independently. Instead, we must lead the effort to develop a fully integrated view of the body, with all parts connected and fully interacting in a realistic way. This paper will present the status of current (2000) plans by the National Space Biomedical Research Institute to initiate research in this area of integrative physiology and medicine. Specifically, three example projects are discussed as potential stepping stones towards the ultimate goal of producing a digital human. These projects relate to developing a functional model of the human musculoskeletal system and the heart.     

Modeling large solar flares

The basic ideas to model the large solar flares are reviewed and illustrated. Some fundamental properties of potential and non-potential fields in the solar atmosphere are recalled. In particular, we consider a classification of the non-potential fields or, more exactly, related electric currents, including reconnecting current layers. The so-called ‘rainbow reconnection’ model is presented with its properties and predictions. This model allows us to understand main features of large flares in terms of reconnection. We assume that in the two-ribbon flares, like the Bastille-day flare, the magnetic separatrices are involved in a large-scale shear photospheric flow in the presence of reconnecting current layers generated by a converging flow.     

Analysis of the Cyclotron Facility calibration and aircraft dosimetry results from the LIULIN-3M instrument.

The LIULIN-3M instrument is a further development of the LIULIN dosimeter-radiometer, used on the MIR spacestation during the 1988-1994 time period. The LIULIN-3M is designed for continuous monitoring of the radiation environment during the BION-12 satellite flight in 1999. A semiconductor detector with 1 mm thickness and cm2 area is contained in the instrument. Pulse high analysis technique is used to determine the energy losses in the detector. The final data from the instrument are the flux and the dose rate for the exposure time and 256 channels of absorbed dose spectra based on the assumption that the particle flux is normal to the detector. The LIULIN-3M instrument was calibrated by proton fluxes with different energies at the Indiana University Cyclotron Facility in June 1997 and had been used for radiation measurements during commercial aircraft flights. The calibration procedure and some flight results are presented in this paper.     

Attractive forces inside a thin nonuniform meteor ring

Attractive forces inside a thin nonuniform meteor ring are found and their features are revealed.     

Super-elite plasma rings and the orbits of planets and satellites isomorphic to the orbits of electrons in the Bohr’s model of the hydrogen atom

This paper continues the series of papers [1–5] and generalizes the previous results to a proto-ring of magnetized plasma whose density decreases in the radial direction. The problem of quantization of the sector and orbital velocities, and of the radii and periods of revolution of elite plasma rings is considered. A new concept of super-elite rings is introduced. Their isomorphism with the orbits of the planets and planetary satellites in the Solar System is proved. This isomorphism also extends to the orbits of electrons in the Bohr’s model of the hydrogen atom.     

Preferred frequencies for SETI observations

SETI observational programs conducted over the last two decades, and most of those planned for the near future, have concentrated on searching for signals at microwave frequencies. Considerations of signal-to-noise ratio at the receiving end indicate that this is the correct approach if the broadcasting society is not concerned with directionality and transmits into a fairly large solid angle. However, if that society desires to transmit only a highly directional beacon, then it is not now possible, given our lack of knowledge of advanced space technology, to predict reliably whether microwave or infrared wavelengths are to be preferred in an optimum search program. Given the realities of current terrestrial technology, either the centimeter or millimeter domain is to be preferred to the infrared, independent of considerations of directionality. In any event, there does not appear to be any cosmically unique (“magic”) frequency at which to conduct SETI.     

Modeling of ionospheric parameter variations in East Asia during the moderate geomagnetic disturbances

The results of modeling of ionospheric disturbances observed in the East Asian region during moderate storms are presented. The numerical model for ionosphere–plasmasphere coupling developed at the ISTP SB RAS is used to interpret the data of observations at ionospheric stations located in the longitudinal sector of 90–130°E at latitudes from auroral zone to equator. There is obtained a reasonable agreement between measurements and modeling results for winter and equinox. In the summer ionosphere, at the background of high ionization by the solar EUV radiation in the quiet geomagnetic period the meridional thermospheric wind strongly impacts the electron concentration in the middle and auroral ionosphere. The consistent calculations of the thermospheric wind permit to obtain the model results which are closer to summer observations. The actual information about the space-time variations of thermosphere and magnetosphere parameters should be taken into account during storms.     

Lithium micro-battery development at the Jet Propulsion Laboratory

Recent successes in the effort to miniaturize spacecraft components using MEMS technology, integrated passive components, and low power electronics have driven the need for very low power, low profile, low mass micro-power sources for micro/nanospacecraft applications. Recent work at JPL has focused upon developing thin film/micro-batteries compatible with temperature sensitive substrates. A process to prepare crystalline LiCoO₂ films with RF sputtering and moderate (<700°C) annealing temperature has been developed. Thin film batteries with cathode films prepared with this process have specific capacities approaching the practical limit for LiCoO₂, with acceptable rate capabilities and discharge voltage profiles. Solid-state micro-scale batteries have also been fabricated with feature sizes on the order of 50 microns     

Effects of bioreactor retention time on aerobic microbial decomposition of CELSS crop residues.

The focus of resource recovery research at the KSC-CELSS Breadboard Project has been the evaluation of microbiologically mediated biodegradation of crop residues by manipulation of bioreactor process and environmental variables. We will present results from over 3 years of studies that used laboratory- and breadboard-scale (8 and 120 L working volumes, respectively) aerobic, fed-batch, continuous stirred tank reactors (CSTR) for recovery of carbon and minerals from breadboard grown wheat and white potato residues. The paper will focus on the effects of a key process variable--bioreactor retention time--on response variables indicative of bioreactor performance. The goal is to determine the shortest retention time that is feasible for processing CELSS crop residues, thereby reducing bioreactor volume and weight requirements. Pushing the lower limits of bioreactor retention times will provide useful data for engineers who need to compare biological and physicochemical components. Bioreactor retention times were manipulated to range between 0.25 and 48 days. Results indicate that increases in retention time lead to a 4-fold increase in crop residue biodegradation, as measured by both dry weight losses and CO2 production. A similar overall trend was also observed for crop residue fiber (cellulose and hemicellulose), with a noticeable jump in cellulose degradation between the 5.3 day and 10.7 day retention times. Water-soluble organic compounds (measured as soluble TOC) were appreciably reduced by more than 4-fold at all retention times tested. Results from a study of even shorter retention times (down to 0.25 days), in progress, will also be presented.