Comparative survival analysis of Deinococcus radiodurans and the haloarchaea Natrialba magadii and Haloferax volcanii exposed to vacuum ultraviolet irradiation

The haloarchaea Natrialba magadii and Haloferax volcanii, as well as the radiation-resistant bacterium Deinococcus radiodurans, were exposed to vacuum UV (VUV) radiation at the Brazilian Synchrotron Light Laboratory. Cell monolayers (containing 10(5) to 10(6) cells per sample) were prepared over polycarbonate filters and irradiated under high vacuum (10(-5) Pa) with polychromatic synchrotron radiation. N. magadii was remarkably resistant to high vacuum with a survival fraction of (3.77±0.76)×10(-2), which was larger than that of D. radiodurans (1.13±0.23)×10(-2). The survival fraction of the haloarchaea H. volcanii, of (3.60±1.80)×10(-4), was much smaller. Radiation resistance profiles were similar between the haloarchaea and D. radiodurans for fluences up to 150?J m(-2). For fluences larger than 150?J m(-2), there was a significant decrease in the survival of haloarchaea, and in particular H. volcanii did not survive. Survival for D. radiodurans was 1% after exposure to the higher VUV fluence (1350?J m(-2)), while N. magadii had a survival lower than 0.1%. Such survival fractions are discussed regarding the possibility of interplanetary transfer of viable microorganisms and the possible existence of microbial life in extraterrestrial salty environments such as the planet Mars and Jupiter's moon Europa. This is the first work to report survival of haloarchaea under simulated interplanetary conditions.     

Sunspot dynamics are reflected in human physiology and pathophysiology

Periodic episodes of increased sunspot activity (solar electromagnetic storms) occur with 10-11 and 5-6 year periodicities and may be associated with measurable biological events. We investigated whether this sunspot periodicity characterized the incidence of Pap smear-determined cervical epithelial histopathologies and human physiologic functions. From January 1983 through December 2003, monthly averages were obtained for solar flux and sunspot numbers; six infectious, premalignant and malignant changes in the cervical epithelium from 1,182,421 consecutive, serially independent, screening Pap smears (59°9″N, 4°29″E); and six human physiologic functions of a healthy man (oral temperature, pulse, systolic and diastolic blood pressure, respiration, and peak expiratory flow), which were measured ～5 times daily during ～34,500 self-measurement sessions (44°56″N, 93°8″W). After determining that sunspot numbers and solar flux, which were not annually rhythmic, occurred with a prominent 10-year and a less-prominent 5.75-year periodicity during this 21-year study span, each biological data set was analyzed with the same curve-fitting procedures. All six annually rhythmic Pap smear-detected infectious, premalignant and malignant cervical epithelial pathologies showed strong 10-year and weaker 5.75-year cycles, as did all six self-measured, annually rhythmic, physiologic functions. The phases (maxima) for the six histopathologic findings and five of six physiologic measurements were very near, or within, the first two quarters following the 10-year solar maxima. These findings add to the growing evidence that solar magnetic storm periodicities are mirrored by cyclic phase-locked rhythms of similar period length or lengths in human physiology and pathophysiology.     

Adsorption of amino acids (ALA, CYS, HIS, MET) on zeolites: fourier transform infrared and Raman spectroscopy investigations

Minerals adsorb more amino acids with charged R-groups than amino acids with uncharged R-groups. Thus, the peptides that form from the condensation of amino acids on the surface of minerals should be composed of amino acid residues that are more charged than uncharged. However, most of the amino acids (74%) in today's proteins have an uncharged R-group. One mechanism with which to solve this paradox is the use of organophilic minerals such as zeolites. Over the range of pH (pH 2.66-4.50) used in these experiments, the R-group of histidine (His) is positively charged and neutral for alanine (Ala), cysteine (Cys), and methionine (Met). In acidic hydrothermal environments, the pH could be even lower than those used in this study. For the pH range studied, the zeolites were negatively charged, and the overall charge of all amino acids was positive. The conditions used here approximate those of prebiotic Earth. The most important finding of this work is that the relative concentrations of each amino acid (X=His, Met, Cys) to alanine (X/Ala) are close to 1.00. This is an important result with regard to prebiotic chemistry because it could be a solution for the paradox stated above. Pore size did not affect the adsorption of Cys and Met on zeolites, and the Si/Al ratio did not affect the adsorption of Cys, His, and Met. ZSM-5 could be used for the purification of Cys from other amino acids (Student-Newman-Keuls test, p<0.05), and mordenite could be used for separation of amino acids from each other (Student-Newman-Keuls test, p<0.05). As shown by Fourier transform infrared (FT-IR) spectra, Ala interacts with zeolites through the [Formula: see text] group, and methionine-zeolite interactions involve the COO, [Formula: see text], and CH(3) groups. FT-IR spectra show that the interaction between the zeolites and His is weak. Cys showed higher adsorption on all zeolites; however, the hydrophobic Van der Waals interaction between zeolites and Cys is too weak to produce any structural changes in the Cys groups (amine, carboxylic, sulfhydryl, etc.); thus, the FT-IR and Raman spectra are the same as those of solid Cys.     

Is the presence of oxygen on an exoplanet a reliable biosignature?

We revisit the validity of the presence of O(2) or O(3) in the atmosphere of a rocky planet as being a biosignature. Up to now, the false positive that has been identified applies to a planet during a hot greenhouse runaway, which is restricted to planets outside the habitable zone (HZ) of the star that are closer to the star. In this paper, we explore a new possibility based on abiotic photogeneration of O(2) at the surface of a planet that could occur inside the HZ. The search for such a process is an active field of laboratory investigation that has resulted from an ongoing interest in finding efficient systems with the capacity to harvest solar energy on Earth. Although such a process is energetically viable, we find it to be a very unlikely explanation for the observation of O(2) or O(3) in the atmosphere of a telluric exoplanet in the HZ. It requires an efficient photocatalyst to be present and abundant under natural planetary conditions, which appears unlikely according to our discussion of known mineral photochemical processes. In contrast, a biological system that synthesizes its constituents from abundant raw materials and energy has the inherent adaptation advantage to become widespread and dominant (Darwinist argument). Thus, O(2) appears to continue to be a good biosignature.     

Prebiotic significance of extraterrestrial ice photochemistry: detection of hydantoin in organic residues

The delivery of extraterrestrial organic materials to primitive Earth from meteorites or micrometeorites has long been postulated to be one of the origins of the prebiotic molecules involved in the subsequent apparition of life. Here, we report on experiments in which vacuum UV photo-irradiation of interstellar/circumstellar ice analogues containing H(2)O, CH(3)OH, and NH(3) led to the production of several molecules of prebiotic interest. These were recovered at room temperature in the semi-refractory, water-soluble residues after evaporation of the ice. In particular, we detected small quantities of hydantoin (2,4-imidazolidinedione), a species suspected to play an important role in the formation of poly- and oligopeptides. In addition, hydantoin is known to form under extraterrestrial, abiotic conditions, since it has been detected, along with various other derivatives, in the soluble part of organic matter of primitive carbonaceous meteorites. This result, together with other related experiments reported recently, points to the potential importance of the photochemistry of interstellar "dirty" ices in the formation of organics in Solar System materials. Such molecules could then have been delivered to the surface of primitive Earth, as well as other telluric (exo-) planets, to help trigger first prebiotic reactions with the capacity to lead to some form of primitive biomolecular activity.     

Design of a canard configured TransCruiser using CEASIOM

CEASIOM is a multidisciplinary software environment for aircraft design that has been developed as part of the European Framework 6 SimSAC project. It closely integrates discipline-specific tools such as those used for CAD, grid generation, CFD, stability analysis and control system design. The environment allows the user to take an initial design from geometry definition and aerodynamics generation through to full six degrees of freedom simulation and analysis. Key capabilities include variable fidelity aerodynamics tools and aeroelasticity modules. The purpose of this paper is to demonstrate the potential of CEASIOM by presenting the results of a Design, Simulate and Evaluate (DSE) exercise applied to a novel, project specific, transonic cruiser configuration called the TCR. The baseline TCR configuration is first defined using conventional methods, which is then refined and improved within the CEASIOM software environment. A wind tunnel model of this final configuration was then constructed, tested and used to verify the results generated using CEASIOM.     

On choice of the blade back profile curvature in transonic turbine cascades

Some design and experimental data on three cascades with the same 12° bending angle for the primary nozzle block of a gas turbine are presented. As is seen from the investigation the most efficient at the transonic velocities of the outward flow are the blades, the back curvature of which in the bevel cut zone decreases to the trailing edge. Also presented is the fact that the blade with the increasing back curvature has noticeably larger losses.     

On the effect of considering more realistic particle shape and mass parameters in MMOD risk assessments

One of the primary mission risks tracked in the development of all spacecraft is that due to micro-meteoroids and orbital debris (MMOD). Both types of particles, especially those larger than 0.1 mm in diameter, contain sufficient kinetic energy due to their combined mass and velocities to cause serious damage to crew members and spacecraft. The process used to assess MMOD risk consists of three elements: environment, damage prediction, and damage tolerance. Orbital debris risk assessments for the Orion vehicle, as well as the Shuttle, Space Station and other satellites use ballistic limit equations (BLEs) that have been developed using high speed impact test data and results from numerical simulations that have used spherical projectiles. However, spheres are not expected to be a common shape for orbital debris; rather, orbital debris fragments might be better represented by other regular or irregular solids. In this paper we examine the general construction of NASA’s current orbital debris (OD) model, explore the potential variations in orbital debris mass and shape that are possible when using particle characteristic length to define particle size (instead of assuming spherical particles), and, considering specifically the Orion vehicle, perform an orbital debris risk sensitivity study taking into account variations in particle mass and shape as noted above. While the results of the work performed for this study are preliminary, they do show that continuing to use aluminum spheres in spacecraft risk assessments could result in an over-design of its MMOD protection systems. In such a case, the spacecraft could be heavier than needed, could cost more than needed, and could cost more to put into orbit than needed. The results obtained in this study also show the need to incorporate effects of mass and shape in mission risk assessment prior to first flight of any spacecraft as well as the need to continue to develop/refine BLEs so that they more accurately reflect the shape and material density variations inherent to the actual debris environment.     

Seasonal variation of surface refractivity over Nigeria

The seasonal variation of surface refractivity over Nigeria was studied using two years in-situ meteorological data from eight locations over Nigeria. The result shows that the surface refractivity generally has higher value during rainy season than dry season at all location studied. The results also show that the value of surface refractivity increases from arid region in the north to the coastal area in south. The results also show that local meteorology plays a very important role in refractivity variation.     

NASA strategic planning for 2000 and beyond

This short paper outlines the United States National Aeronautics and Space Administration's development of its 2000 Strategic Plan.