Increased BLSS closure using mineralized human waste in plant cultivation on a neutral substrate

The purpose of this work was to study the full-scale potential use of human mineralized waste (feces and urine) as a source of mineral elements for plant cultivation in a biological life support system (BLSS). Plants that are potential candidates for a photosynthesizing link were grown on a neutral solution containing human mineralized waste. Spring wheat Triticum aestivum L., peas Pisum sativum L. Ambrosia cultivar and leaf lettuce Lactuca sativa L., Vitaminny variety, were used. The plants were grown hydroponically on expanded clay aggregates in a vegetation chamber in constant environmental conditions. During plant growth, a determined amount of human mineralized waste was added daily to the nutrient solution. The nutrient solution remained unchanged throughout the vegetation period. Estimated plant requirements for macro-elements were based on a total biological productivity of 0.04 kg day⁻¹ m⁻². As the plant requirements for potassium exceeded the potassium content of human waste, a water extract of wheat straw containing the required amount of potassium was added to the nutrient solution. The Knop’s solution was used in the control experiments.     

Longitudinal structure of ballooning MHD disturbances in a model magnetosphere

Large-scale toroidal Pc5 pulsations are commonly treated as Alfven oscillations of a magnetic field line. According to observations, their longitudinal structure is described well by theory. At the same time, the longitudinal structure of azimuthal small-scale poloidal Pc5 pulsations is virtually unknown. These pulsations are associated with ballooning disturbances described by a system of coupled equations for Alfvenic and slow magnetosonic (SMS) modes. In this work, the Voigt model is used to describe the equilibrium finite-pressure plasma configuration in an inhomogeneous magnetosphere plasma in a curved magnetic field. Spectral characteristics and the spatial structure of natural ballooning modes are calculated for this model. The model calculations demonstrate the possibility of different longitudinal scales for transverse and longitudinal magnetic components of oscillations near the top of the field line.     

Analytical solution for continuous-time Kalman tracking filterswith colored measurement noise in frequency domain

New expressions are given for analytical solutions to the steady-state Kalman gains of the two-state exponentially correlated velocity (ECV) and the three-state exponentially correlated acceleration (ECA) tracking filters with position measurements by using spectral factorization method. The measurement colored noise model is characterized by a correlation time 1/λ. The vehicle oscillations such as wind-induced-bending is also considered in the modeling of the system which leads to the most generalized state transition matrix     

SPOT-2 and TOPEX/Poseidon precise orbit determination from DORIS doppler tracking

The French earth observation satellite SPOT-2 has served as a testbed for precise orbit determination from DORIS doppler tracking in anticipation of the TOPEX/Poseidon mission. Using the most up-to-data gravity field model, JGM-2, a radial orbit accuracy of about 2–9 cm was achieved, with an rms of fit of the tracking data of about 0.64 mm/s. Furthermore, it was found that the coordinates of the ground stations can be determined with an accuracy of the order of 2–5 cm after removal of common rotations, and translations.

Using a slightly different model for atmospheric drag, but the same gravity model, precise orbits of TOPEX/Poseidon from DORIS tracking data were determined with a radial orbit accuracy of the order of 4–5 cm, which is far within the 13 cm mission requirement. This conclusion is based on the analysis of 1-day overlap of successive 11-day orbits, and the comparisons with orbits computed from satellite laser tracking (SLR) and from the combination of SLR and DORIS tracking. Results indicate a consistency between the different orbits of 1–4 cm, 4–20 cm, and 6–13 cm in the radial, cross-track, and along-track directions, respectively. The residual rms is about 4–5 cm for SLR data and 0.56 mm/s for DORIS tracking. These numbers are roughly twice as large as the system noise levels, reflecting the fact that there are still some modeling errors left.     

Response of the electron energy distribution to an artificially emitted electron beam: Apex experiment

The paper is based on the electron and ion energy spectra measurement on board the main spacecraft of the APEX mission. During the active phase of the experiment an intense electron beam was emitted from the main satellite. The basic cycle of the electron injection is formed by current pulses of different frequencies, duration and intensity. The spacecraft potential changes due to the gun operation were compensated by a low energy Xe plasma generator. The data show that the response of the environment to the beam emission depends not only on injection parameters but on the spacecraft position and orientation with respect to the magnetic field as well. The typical response is an increase of the intensity of the low energy (less than 1 keV) electrons in all directions. In addition, strong field aligned fluxes of electrons and/or ions are observed with energies below the gun energy. An attempt to classify different types of response and to find possible mechanisms which can explain the observed phenomena is made in the present paper.     

The Evolving Sigmoid: Evidence for Magnetic Flux Ropes in the Corona Before, During, and After CMES

It is generally accepted that the energy that drives coronal mass ejections (CMEs) is magnetic in origin. Sheared and twisted coronal fields can store free magnetic energy which ultimately is released in the CME. We explore the possibility of the specific magnetic configuration of a magnetic flux rope of field lines that twist about an axial field line. The flux rope model predicts coronal observables, including heating along forward or inverse S-shaped, or sigmoid, topological surfaces. Therefore, studying the observed evolution of such sigmoids prior to, during, and after the CME gives us crucial insight into the physics of coronal storage and release of magnetic energy. In particular, we consider (1) soft-X-ray sigmoids, both transient and persistent; (2) The formation of a current sheet and cusp-shaped post-flare loops below the CME; (3) Reappearance of sigmoids after CMEs; (4) Partially erupting filaments; (5) Magnetic cloud observations of filament material.     

DNA fragmentation by charged particle tracks.

High-LET (linear energy transfer) charged particles induce DNA double-strand breaks (DSB) in a non-random fashion in mammalian cells. The clustering of DSB, probably determined by track structure as well as chromatin conformation, results in an excess of small- and intermediate-sized DNA fragments. DNA fragmentation in normal human fibroblasts (GM5758) was analyzed by pulsed-field gel electrophoresis after irradiation with photons (60Co) or 125 keV/micrometers nitrogen ions. Compared to conventional DSB analysis, i.e. assays only measuring the fraction of DNA smaller than a single threshold, the relative biological effectiveness (RBE) for DSB induction increased with 100%. Further, the size distribution of DNA fragments showed a significant dependence on radiation quality, with an excess of fragments up to 1 Mbp. Irradiation of naked genomic DNA without histone proteins increased the DSB yields 25 and 13 times for photons and nitrogen ions, respectively. The results suggest possible roles of both track structure and chromatin organization in the distribution of DNA double-strand breaks along the chromosome.     

High temperature effect on microflora of radish root-inhabited zone and nutrient solutions for radish growth.

The effect of high temperatures (35 and 45 degrees C) on microflora of the root zone of radish plants grown in phytotron was evaluated by the response of microorganisms from 9 indicator groups. Phytotron air temperature elevated to 35 degrees C for 20 hours caused no significant changes in qualitative and quantitative composition of the root microflora in experimental plants. By the end of the experiment, the species diversity of microflora had changed. The amount of phytopathogenic microorganisms decreased which can be interpreted as more stable co-existence of microflora with plants. The numbers of microbes from other indicator groups was in dynamic equilibrium. The plants' condition did not deteriorate either. Exposure to the temperature of 45 degrees C for 7 hours have been found to change the numbers and species diversity in the radish root zone microflora. The microorganisms were observed to increase their total numbers at the expense of certain indicator groups. Bacteria increased spore forms at the stage of spores. Colon bacillus bacteria of increased their numbers by the end of experiment by an order. By the end of experiment the roots of experiment plants had microscopic fungi from Mucor, Aspergillus, Trichoderma, Cladosporium genera. The observed changes in the microbial complex seem to be associated with the changes of root emissions and general deterioration of the plants' condition. It is suggested that the response of the microorganisms can be indicative of the condition of plants under investigation.     

Issues of exploration: human health and wellbeing during a mission to Mars.

Today, the tools are in our hands to enable us to travel away from our home planet and become citizens of the solar system. Even now, we are seriously beginning to develop the robust infrastructure that will make the 21st century the Century of Space Travel. But this bold step must be taken with due concern for the health, safety and wellbeing of future space explorers. Our long experience with space biomedical research convinces us that, if we are to deal effectively with the medical and biomedical issues of exploration, then dramatic and bold steps are also necessary in this field. We can no longer treat the human body as if it were composed of muscles, bones, heart and brain acting independently. Instead, we must lead the effort to develop a fully integrated view of the body, with all parts connected and fully interacting in a realistic way. This paper will present the status of current (2000) plans by the National Space Biomedical Research Institute to initiate research in this area of integrative physiology and medicine. Specifically, three example projects are discussed as potential stepping stones towards the ultimate goal of producing a digital human. These projects relate to developing a functional model of the human musculoskeletal system and the heart.     

Mechanisms of generation and dynamics of HF and LF emissions in the experiments with electron beams injected into the earth’s ionosphere

Mechanisms of generation and dynamics of HF and LF emissions related to an electron beam injection from a spacecraft into the ionosphere are studied. The choice of the source and region of emission is substantiated. The plasma current neutralizing the spacecraft charge and the region of spatial charge in the vicinity of the spacecraft are chosen. Mechanisms of the generation of HF and LF emissions are proposed and their characteristics are determined. For the considered ranges of emission, the explanation of the facts related to the difficulties in interpretation of the amplitude value observed experimentally and the behavior of its envelope are presented. It is shown that the results obtained in this work agree well with the data of the conducted experiments.