Changes in spectral reflectance of wheat leaves in response to specific macronutrient deficiency.

In wheat (Triticum aestivum L.) plants, deficiency of an essential element may drastically affect growth, appearance, and most importantly yield. Wheat, the focus of this study, is one of the crops studied in the CELSS program. Information about nutrient deficiencies in crops grown in controlled environment is essential to optimize food productivity. The main objective of this study was to determine whether deficiency of Nitrogen (N), Phosphorus (P), Potassium (K), Calcium (Ca) and Magnesium (M) alters spectral reflectance properties of wheat leaves. Plants were grown in the greenhouse and growth chamber, in a modified Hoagland's nutrient solution. Spectral reflectance of fully expanded wheat leaves from 280 to 1100 nm, nutrient concentrations (N, P, K, and Ca) and chlorophyll (Chl) were determined when deficiency symptoms were first evident (approximately 6-7 weeks). Chlorophyll content and fresh and dry weight, were used to assess the severity of the nutrient stress. All nutrient deficiencies affected chlorophyll content and generally increased reflectance in the visible (VIS) 400-700 nm and infrared (IR) 700-1100 nm ranges. Magnesium and nitrogen deficiencies had the most pronounced effect on chlorophyll concentration height, and reflectance. All macronutrient deficiencies tested reduced chlorophyll concentration, increase reflectance in the visible range and caused a shift in the position of the red edge (the point of maximum slope on the reflectance spectrum of vegetation between red and near-infrared wavelengths) toward shorter or longer wavelengths; depending upon the element. In the greenhouse, N and Mg induced the greatest increase in reflectance of 33% and 25% in the VI range and 86% and 53% in the IR range, respectively. However, in the growth chamber, an increase of 97% and 25% occurred in the VI range, and 20% and 33% in the IR range, respectively. In the IR range in the growth chamber, P, K, and Ca deficiency caused a reduction in reflectance (412-770 nm). This research indicates that mineral deficiencies and reflectance are not specific to one environment and could have important implications for the design of CELSS in space, and perhaps the future of terrestrial agriculture.     

Modern aspects of planetary protection and requirements to sterilization of space hardware.

The viewpoint of working group of Russian experts on the problem of planetary protection for future manned and unmanned Mars mission is presented. Recent data of Martian environment and on survival of terrestrial microorganisms in extreme conditions were used for detailed analysis and overview of planetary protection measures in regard to all possible flight situations including accidental landing. The special emphasis on "Mars-94" mission was done. This analysis resulted in revised formulation of spacecraft sterilization requirements and possible measures for their best implementation. New general combined approach to spacecraft sterilization was proposed. It includes penetrating radiation and heat treatment of spacecraft parts and components which is to be carried out before the final assembly of spacecraft and gaseous radiation sterilization of the whole spacecraft during the flight to Mars (or from Mars for return missions).     

Surface tension-driven flows in horizontal liquid metal layers

The problem of surface tension-driven flows in horizontal liquid layers has been studied experimentally, and theoretically by direct numerical simulation and small perturbation analysis. We focus our attention on situations in which the depth of the fluid (liquid tin; small Prandtl number, Pr=0.015) is small enough to ensure the predominance of the surface tension forces over those due to the buoyancy. The surface velocity has been experimentally obtained for liquid tin layer with various aspect ratio (length to height) in the range 5<A<83. The thermal gradients are ranged from 5 to 40°K/cm. In the numerical study, the Navier-Stokes and energy equations are solved by an efficient finite difference technique. The parameters governing the flow behaviour in the liquid are varied to determine their effects on thermocapillary convection: the Reynolds number 10<Re<210⁴ and the aspect ratio 2<A<25; with Pr kept constant at Pr=0.015. The linear eigenequation resulting from small spatial disturbances of the Couette flow solution is solved using an Tau-Chebyshev approximation. A notable feature of the theoretical study is the totally different end circulations. In the region near the cold wall a multicell structure is evident. This agrees with the eigensolution which is of complex type, indicating spatial periodicity. In the hot wall region the flow is accelerated to reach the velocity value for the fully-developed Couette flow which is reached under conditions such as Re/A<20. The transition from viscous to boundary layer regime occurs for a critical value (Re/A)_c of nearly about 200, as deduced from the numerical and experimental results.     

VEGA three-channel spectrometer experiment: Spectroscopy of comet Halley in the visible and near-ultraviolet ranges

A total of 3600 spectra of Comet Halley in the 275–710 nm were obtained on March, 8, 9, 10 and 11, 1986, from the VEGA 2 spacecraft. The emissions of OH, NH, CN, C₃, CH, C₂, NH₂ and H₂O⁺ are identified. From the OH intensity in the (0,0) band: 1.1 Megarayleigh at 5400 km from the nucleus, it can be inferred that the OH production rate was (1.4 ± 0.5)×10³⁰ molecules s⁻¹. The NH, C₃, CH and NH₂ bands became comparatively more intense at distances from the nucleus shorter than 3000km. At 06:40 U.T. when the instrument field of view was 6000×4500 km, two jets were observed. Spectra from the jets show significant differences with other spectra. Inside a jet NH, C₃ and NH₂ are comparatively more intense and the rotational distributions of OH, CN and C₂ are strongly distorted. This shows that part of the observed emissions probably comes from radicals directly produced in the excited state during the initial process of photolysis of the parent molecules.     

A first step towards proton flux forecasting

We present a preliminary version of a potential tool for real time proton flux prediction which provides proton flux profiles and cumulative fluence profiles at 0.5 and 2 MeV of solar energetic particle events, from their onset up to the arrival of the interplanetary shock at the spacecraft position (located at 1 or 0.4 AU). Based on the proton transportation model by Lario et al. [Lario, D., Sanahuja, B., Heras, A.M. Energetic particle events: efficiency of interplanetary shocks as 50 keV E < 100 MeV proton accelerators. Astrophys. J. 509, 415–434, 1998] and the magnetohydrodynamic shock propagation model of Wu et al. [Wu, S.T., Dryer, M., Han, S.M. Non-planar MHD model for solar flare-generated disturbances in the Heliospheric equatorial plane. Sol. Phys. 84, 395–418, 1983], we have generated a database containing “synthetic” profiles of the proton fluxes and cumulative fluences of 384 solar energetic particle events. We are currently validating the applicability of this code for space weather forecasting by comparing the resulting “synthetic” flux profiles with those of several real events.     

Transient fast reconnection in dynamic current sheets with anomalous resistivity

Two-dimensional compressible magnetohydrodynamic simulations of current sheet dynamics under the influence of multiple anomalous resistivity areas and slight asymmetries are presented. Following induced tearing and multiple coalescence, a plasmoid is formed and accelerated. Dominant X-points drive the dynamical evolution and lead to transient occurrence of a Petschek-like reconnection geometry. The dependence of current density extrema, plasmoid bulk velocity and maximum reconnection rate on the Lundquist number is examined.     

Metabolic changes in rats subjected to space flight for 18.5 days in the biosatellite Cosmos 936

From an investigation of the activity of six glucocorticoid dependent liver enzymes, the existence of chronic, transient, stress-induced hypercorticosteronaemia during flight is probable. This hypercorticosteronaemia arises from weightlessness and induces gluconeogenesis. Weightlessness also caused substantial increases in liver glycogen level. The increased lipolytic activity and that of lipoprotein lipase in several groups of animals could be interpreted as enhancement of fat mobilization and utilization under the influence of stress. As this latter enhancement was also found in ground-based controls, it may have been due to the stress of handling rather than to space flight per se.     

Survival in extreme dryness and DNA-single-strand breaks.

A wide variety of organisms (the so-called "anhydrobiotes') is able to survive long periods of time in a state of utmost dehydration and can thus survive in extremely dry environments including artificially imposed or space vacuum. Known strategies of survival include the accumulation of certain polyols, especially disaccharides, which help prevent damage to membranes and proteins. Here we report that DNA in vacuum-dried spores is damaged to a very substantial degree by processes leading to DNA strand breaks. Most of these lesions are obviously repaired during germination, but extensive damage to DNA and enzymes after long exposure times (months to years) finally diminish the chances of survival.     

Biosphere 2 Test Module: a ground-based sunlight-driven prototype of a closed ecological life support system.

Constructed in 1986, the Biosphere 2 Test Module has been used since the end of that year for closed ecological systems experiments. It is the largest closed ecological facility ever built, with a sealed variable volume of some 480 cubic meters. It is built with a skin of steel spaceframes with double-laminated glass panels admitting about 65 percent Photosynthetically Active Radiation (PAR). The floor is of welded steel and there is an underground atmospheric connection via an air duct to a variable volume chamber ("lung") permitting expansion and contraction of the Test Module's air volume caused by changes in temperature and barometric pressure, which causes a slight positive pressure from inside the closed system to the outside thereby insuring that the very small leakage rate is outward. Several series of closed ecological system investigations have been carried out in this facility. One series of experiments investigated the dynamics of higher plants and associated soils with the atmosphere under varying light and temperature conditions. Another series of experiments included one human in the closed system for three, five and twenty-one days. During these experiments the Test Module had subsystems which completely recycled its water and atmosphere; all the human dietary needs were produced within the facility, and all wastes were recycled using a marsh plant/microbe system. Other experiments have examined the capability of individual component systems used, such as the soil bed reactors, to eliminate experimentally introduced trace gases. Analytic systems developed for these experiments include continuous monitors of eleven atmospheric gases in addition to the complete gas chromatography mass spectrometry (GCMS) examinations of potable, waste system and irrigation water quality.