Cellular communication and “non-targeted effects”: Modelling approaches

During the last decade, a large number of experimental studies on the so-called “non-targeted effects”, in particular bystander effects, outlined that cellular communication plays a significant role in the pathways leading to radiobiological damage. Although it is known that two main types of cellular communication (i.e. via gap junctions and/or molecular messengers diffusing in the extra-cellular environment, such as cytokines, NO etc.) play a major role, it is of utmost importance to better understand the underlying mechanisms, and how such mechanisms can be modulated by ionizing radiation. Though the “final” goal is of course to elucidate the in vivo scenario, in the meanwhile also in vitro studies can provide useful insights. In the present paper we will discuss key issues on the mechanisms underlying non-targeted effects and cell communication, for which theoretical models and simulation codes can be of great help. In this framework, we will present in detail three literature models, as well as an approach under development at the University of Pavia. More specifically, we will first focus on a version of the “State-Vector Model” including bystander-induced apoptosis of initiated cells, which was successfully fitted to in vitro data on neoplastic transformation supporting the hypothesis of a protective bystander effect mediated by apoptosis. The second analyzed model, focusing on the kinetics of bystander effects in 3D tissues, was successfully fitted to data on bystander damage in an artificial 3D skin system, indicating a signal range of the order of 0.7–1 mm. A third model for bystander effect, taking into account of spatial location, cell killing and repopulation, showed dose–response curves increasing approximately linearly at low dose rates but quickly flattening out for higher dose rates, also predicting an effect augmentation following dose fractionation. Concerning the Pavia approach, which can model the release, diffusion and depletion/degradation of candidate signals (e.g. cytokines) travelling in the extra-cellular environment, the good agreement with ad hoc experimental data obtained in our laboratory validated the adopted approach, which in the future can be applied also to other candidate signals.     

A mars communication constellation for human exploration and network science

This paper analyses the possibility of exploiting a small spacecrafts constellation around Mars to ensure a complete and continuous coverage of the planet, for the purpose of supporting future human and robotic operations and taking advantage of optical transmission techniques. The study foresees such a communications mission to be implemented at least after 2020 and a high data-rate requirement is imposed for the return of huge scientific data from massive robotic exploration or to allow video transmissions from a possible human outpost.     

Italian industry's approach to space

European space activities began in the 1960's. The development of what was initially scientific research and which later spread to the enthusiastic involvement of Europe's most advanced industry is by now familiar history. The organization of ELDO and ESRO, which eventually gave way to today's ESA, occured in a span of less than a decade. Many major European countries also commenced their national space programmes during this period. This report examines the space initiatives taken by Italian industry.     

The PRISMA payload optomechanical design, a high performance instrument for a new hyperspectral mission

PRISMA (PRecursore IperSpettrale della Missione Applicativa) hyperspectral instrument is an advanced hyperspectral sensor including a panchromatic camera at medium resolution. The instrument is the focus of the new Earth observation mission that a consortium of Italian companies has started developing under contract of Italian Space Agency. Key features of the instrument are the very high requirement for signal-to-noise and the high quality of data that have to be provided. To meet these demanding figures the optical system has been based on a high transmittance optical system, including a single mirror telescope and two prism spectrometers based on an innovative concept to minimize number of optical elements, while high performance detectors have been chosen for the photon detection. To provide the required data quality for the entire mission lifetime an accurate calibration unit (radiometric and spectral) will be included in the instrument optomechanical assembly. The thermo-mechanical design of the instrument is based on innovative concepts, considering that the use of prism spectrometers implies a tight control of temperature variations to guarantee the stability of all instrument features once in orbit. The presented paper describes the concepts and design principle of the optomechanical assembly of the instrument, at the present status of development.     

A hybrid,self-adjusting search algorithm for optimal space trajectory design

The aim of the present paper is to propose a hybrid, self adjusting, search algorithm for space trajectory optimization. By taking advantage of both direct and indirect methods, the present algorithm allows the finding of the optimal solution through the introduction of some new control parameters, whose number is smaller than that of the Lagrange multipliers, and whose range is bounded. Eventually, the optimal solution is determined by means of an iterative self-adjustment of the search domain occurring at “runtime”, without any correction by an external user. This new set of parameters can be found through a reduction process of the degrees of freedom, obtained through the transversality conditions before entering the search loop. Furthermore, such a process shows that Lagrange multipliers are subject to a deep symmetry mirroring the features of the state vector. The algorithm reliability and efficiency is assessed through some test cases, and by reproducing some optimal transfer trajectories: a full three-dimensional, minimum time Mars mission, an optimal transfer to Jupiter, and finally an injection into a circular Moon orbit.     

JIRAM,the Jovian Infrared Auroral Mapper

JIRAM is an imager/spectrometer on board the Juno spacecraft bound for a polar orbit around Jupiter. JIRAM is composed of IR imager and spectrometer channels. Its scientific goals are to explore the Jovian aurorae and the planet’s atmospheric structure, dynamics and composition. This paper explains the characteristics and functionalities of the instrument and reports on the results of ground calibrations. It discusses the main subsystems to the extent needed to understand how the instrument is sequenced and used, the purpose of the calibrations necessary to determine instrument performance, the process for generating the commanding sequences, the main elements of the observational strategy, and the format of the scientific data that JIRAM will produce.     

Validation of numerical prediction of dynamic derivatives: The DLR-F12 and the Transcruiser test cases

The dynamic derivatives are widely used in linear aerodynamic models in order to determine the flying qualities of an aircraft: the ability to predict them reliably, quickly and sufficiently early in the design process is vital in order to avoid late and costly component redesigns. This paper describes experimental and computational research dealing with the determination of dynamic derivatives carried out within the FP6 European project SimSAC. Numerical and experimental results are compared for two aircraft configurations: a generic civil transport aircraft, wing-fuselage-tail configuration called the DLR-F12 and a generic Transonic CRuiser, which is a canard configuration. Static and dynamic wind tunnel tests have been carried out for both configurations and are briefly described within this paper. The data generated for both the DLR-F12 and TCR configurations include force and pressure coefficients obtained during small amplitude pitch, roll and yaw oscillations while the data for the TCR configuration also include large amplitude oscillations, in order to investigate the dynamic effects on nonlinear aerodynamic characteristics. In addition, dynamic derivatives have been determined for both configurations with a large panel of tools, from linear aerodynamic (Vortex Lattice Methods) to CFD. This work confirms that an increase in fidelity level enables the dynamic derivatives to be calculated more accurately. Linear aerodynamics tools are shown to give satisfactory results but are very sensitive to the geometry/mesh input data. Although all the quasi-steady CFD approaches give comparable results (robustness) for steady dynamic derivatives, they do not allow the prediction of unsteady components for the dynamic derivatives (angular derivatives with respect to time): this can be done with either a fully unsteady approach i.e. with a time-marching scheme or with frequency domain solvers, both of which provide comparable results for the DLR-F12 test case. As far as the canard configuration is concerned, strong limitations for the linear aerodynamic tools are observed. A key aspect of this work are the acceleration techniques developed for CFD methods, which allow the computational time to be dramatically reduced while providing comparable results.     

Responses of haloarchaea to simulated microgravity

Various effects of microgravity on prokaryotes have been recognized in recent years, with the focus on studies of pathogenic bacteria. No archaea have been investigated yet with respect to their responses to microgravity. For exposure experiments on spacecrafts or on the International Space Station, halophilic archaea (haloarchaea) are usually embedded in halite, where they accumulate in fluid inclusions. In a liquid environment, these cells will experience microgravity in space, which might influence their viability and survival. Two haloarchaeal strains, Haloferax mediterranei and Halococcus dombrowskii, were grown in simulated microgravity (SMG) with the rotary cell culture system (RCCS, Synthecon). Initially, salt precipitation and detachment of the porous aeration membranes in the RCCS were observed, but they were avoided in the remainder of the experiment by using disposable instead of reusable vessels. Several effects were detected, which were ascribed to growth in SMG: Hfx. mediterranei's resistance to the antibiotics bacitracin, erythromycin, and rifampicin increased markedly; differences in pigmentation and whole cell protein composition (proteome) of both strains were noted; cell aggregation of Hcc. dombrowskii was notably reduced. The results suggest profound effects of SMG on haloarchaeal physiology and cellular processes, some of which were easily observable and measurable. This is the first report of archaeal responses to SMG. The molecular mechanisms of the effects induced by SMG on prokaryotes are largely unknown; haloarchaea could be used as nonpathogenic model systems for their elucidation and in addition could provide information about survival during lithopanspermia (interplanetary transport of microbes inside meteorites).     

Lense-Thirring Precession in the Astrophysical Context

This paper surveys some of the astrophysical environments in which the effects of Lense-Thirring precession and, more generally, frame dragging are expected to be important. We concentrate on phenomena that can probe in situ the very strong gravitational field and single out Lense-Thirring precession in the close vicinity of accreting neutron stars and black holes: these are the fast quasi periodic oscillations in the X-ray flux of accreting compact objects. We emphasise that the expected magnitude of Lense-Thirring/frame dragging effects in the regions where these signals originate are large and thus their detection does not pose a challenge; rather it is the interpretation of these phenomena that needs to be corroborated through deeper studies. Relativistic precession in the spin axis of radio pulsars hosted in binary systems hosting another neutron star has also been measured. The remarkable properties of the double pulsar PSR J0737–3039 has opened a new perspective for testing the predictions of general relativity also in relation to the precession of spinning bodies.     

Buffering Fuzzy Maps in GIS

In this paper, we show how standard GIS operations like the complement, union, intersection, and buffering of maps can be made more flexible by using fuzzy set theory. In particular, we present a variety of algorithms for operations on fuzzy raster maps, focusing on buffer operations for such maps. Furthermore, we show how widely-available special-purpose hardware (in particular, z-buffering in graphics hardware) can be used for supporting buffer operations in fuzzy geographic information systems (GIS).