Presynaptic transporter-mediated release of glutamate evoked by the protonophore FCCP increases under altered gravity conditions

High-affinity Na⁺-dependent glutamate transporters of the plasma membrane mediate the glutamate uptake into neurons, and thus maintain low levels of extracellular glutamate in the synaptic cleft. The study focused on the release of glutamate by reversal of Na⁺-dependent glutamate transporters from rat brain nerve terminals (synaptosomes) under conditions of centrifuge-induced hypergravity. Flow cytometric analysis revealed similarity in the size and cytoplasmic granularity between synaptosomal preparations obtained from control and G-loaded animals (10 G, 1 h). The release of cytosolic l-[¹⁴C]glutamate from synaptosomes was evaluated using the protonophore FCCP, which dissipated synaptic vesicle proton gradient, thus synaptic vesicles were not able to keep glutamate inside and the latter enriched cytosol. FCCP per se induced the greater release of l-[¹⁴C]glutamate in hypergravity as compared to control (4.8 ± 1.0% and 8.0 ± 1.0% of total label). Exocytotic release of l-[¹⁴C]glutamate evoked by depolarization was reduced down to zero after FCCP application under both conditions studied. Depolarization stimulated release of cytosolic l-[¹⁴C]glutamate from synaptosomes preliminary treated with FCCP was considerably increased from 27.0 ± 2.2% of total label in control to 35.0 ± 2.3% in hypergravity. Non-transportable inhibitor of glutamate transporter dl-threo-β-benzyloxyaspartate was found to significantly inhibit high-KCl and FCCP-stimulated release of l-[¹⁴C]glutamate, confirming the release by reversal of glutamate transporters. The enhancement of transporter-mediated release of glutamate in hypergravity was found to result at least partially from the inhibition of the activity of Na/K-ATPase in the plasma membrane of synaptosomes. We suggested that hypergravity-induced alteration in transporter-mediated release of glutamate indicated hypoxic injury of neurons.     

The origin of the prompt optical emission in GRB 060111B

The detection of a bright optical emission measured with good temporal resolution during the prompt phase makes GRB 060111B a rare event that is especially useful for constraining theories of the prompt optical emission. Comparing this burst with other GRBs with evidence of optical peaks, we find that the optical peak epoch (t_p) is anti-correlated with the high energy burst energetic assuming an isotropic energy release (E_iso) in agreement with Liang et al. (2009), and that the steeper is the post-peak afterglow decay, the less is the agreement with the correlation. GRB 060111B is among the latters and it does not match the correlation. The Cannonball scenario is also discussed and we find that this model cannot be excluded for GRB 060111B.     

A model of the effects of heavy ion radiation on human tissue

In heavy ion radiotherapy and space travel humans are exposed to energetic heavy ions (C, Si, Fe and others). This type of irradiation often produces more severe biological effects per unit dose than more common X-rays. A new Monte Carlo model generates a physical space with the complex geometry of human tissue or a cell culture based model of tissue, which is affected by the passage of ionizing radiation. For irradiation, the model relies on a physical code for the ion track structure; for tissues, cellular maps are derived from two- or three-dimensional confocal microscopy images using image segmentation algorithm, which defines cells as pixilated volumes. The model is used to study tissue-specific statistics of direct ion hits and the remote ion action on cells. As an application of the technique, we considered the spatial pattern of apoptotic cells after heavy ion irradiation. The pattern of apoptosis is modeled as a stochastic process, which is defined by the action cross section taken from available experimental data. To characterize the degree of apoptosis, an autocorrelation function that describes the spatial correlation of apoptotic cells is introduced. The values of the autocorrelation function demonstrate the effect of the directionality of the radiation track on the spatial arrangements of inactivated cells in tissue. This effect is intrinsic only to high linear-energy-transfer radiation.     

Modeling of ionospheric scintillation at low-latitude

The presence and movement of plasma density fluctuations in the F-region of the ionosphere are studied by monitoring phase and amplitude of radio waves propagating through the region. In this paper, we have used weak scattering theory and assumed the plasma density fluctuations to behave like phase changing diffraction screen. Appropriate relations for scintillation index S₄, and phase variance δ? are derived and computed for different parameters of the plasma density irregularities of the ionosphere. SROSS-C2 satellite in situ measurements of plasma density fluctuations, which provide direct information about the structure and morphology of irregularities that are responsible for scintillation of radio waves, were used first time to develop a scintillation model for low latitude. It is observed that the scintillation index S₄ and phase variance δ? depends on the strength of the plasma turbulence. Finally, the results obtained from modeling are compared and discussed with the available recent results.     

Results of solar observations by the CORONAS-F payload

The CORONAS-F mission experiments and results have been reviewed. The observations with the DIFOS multi-channel photometer in a broad spectral range from 350 to 1500 nm have revealed the dependence of the relative amplitudes of p-modes of the global solar oscillations on the wavelength that agrees perfectly well with the earlier data obtained in a narrower spectral ranges. The SPIRIT EUV observations have enabled the study of various manifestations of solar activity and high-temperature events on the Sun. The data from the X-ray spectrometer RESIK, gamma spectrometer HELICON, flare spectrometer IRIS, amplitude–temporal spectrometer AVS-F, and X-ray spectrometer RPS-1 have been used to analyze the X- and gamma-ray emission from solar flares and for diagnostics of the flaring plasma. The absolute and relative content of various elements (such as potassium, argon, and sulfur) of solar plasma in flares has been determined for the first time with the X-ray spectrometer RESIK. The Solar Cosmic Ray Complex monitored the solar flare effects in the Earth’s environment. The UV emission variations recorded during solar flares in the vicinity of the 120-nm wavelength have been analyzed and the amplitude of relative variations has been determined.     

Normalized ionization yield function for various nuclei obtained with full Monte Carlo simulations

Several recent results important for production of ion pairs in the Earth atmosphere by various primary cosmic ray nuclei are presented. The direct ionization by various primary cosmic ray nuclei is explicitly obtained. The longitudinal profile of atmospheric cascades is sensitive to the energy and mass (charge) of the primary particle. In this study different cosmic ray nuclei are considered as primaries, namely Helium, Oxygen and Iron nuclei. The cosmic ray induced ionization is obtained on the basis of CORSIKA 6.52 code simulations using FLUKA 2006 and QGSJET II hadronic interaction models. The energy of the primary particles is normalized to GeV per nucleon. In addition, the ionization yield function Y is normalized as ion pair production per nucleon. The obtained ionization yield functions Y for various primaries are compared. The presented results and their application are discussed.     

Changes in hyperspectral reflectance signatures of lettuce leaves in response to macronutrient deficiencies

The adaptation of specific remote sensing and hyperspectral analysis techniques for the determination of incipient nutrient stress in plants could allow early detection and precision supplementation for remediation, important considerations for minimizing mass of advanced life support systems on space station and long term missions. This experiment was conducted to determine if hyperspectral reflectance could be used to detect nutrient stress in Lactuca sativa L. cv. Black Seeded Simpson. Lettuce seedlings were grown for 90 days in a greenhouse or growth chamber in vermiculite containing modified Hoagland’s nutrient solution with key macronutrient elements removed in order to induce a range of nutrient stresses, including nitrogen, phosphorus, potassium, calcium, and magnesium. Leaf tissue nutrient concentrations were compared with corresponding spectral reflectances taken at the end of 90 days. Spectral reflectances varied with growing location, position on the leaf, and nutrient deficiency treatment. Spectral responses of lettuce leaves under macronutrient deficiency conditions showed an increase in reflectance in the red, near red, and infrared wavelength ranges. The data obtained suggest that spectral reflectance shows the potential as a diagnostic tool in predicting nutrient deficiencies in general. Overlapping of spectral signatures makes the use of wavelengths of narrow bandwidths or individual bands for the discrimination of specific nutrient stresses difficult without further data processing.     

Clouds and Earth Radiant Energy System (CERES), a review: Past,present and future

The Clouds and Earth Radiant Energy System (CERES) project’s objectives are to measure the reflected solar radiance (shortwave) and Earth-emitted (longwave) radiances and from these measurements to compute the shortwave and longwave radiation fluxes at the top of the atmosphere (TOA) and the surface and radiation divergence within the atmosphere. The fluxes at TOA are to be retrieved to an accuracy of 2%. Improved bidirectional reflectance distribution functions (BRDFs) have been developed to compute the fluxes at TOA from the measured radiances with errors reduced from ERBE by a factor of two or more. Instruments aboard the Terra and Aqua spacecraft provide sampling at four local times. In order to further reduce temporal sampling errors, data are used from the geostationary meteorological satellites to account for changes of scenes between observations by the CERES radiometers.     

Potential of salt-accumulating and salt-secreting halophytic plants for recycling sodium chloride in human urine in bioregenerative life support systems

This study addresses the possibility of growing different halophytic plants on mineralized human urine as a way to recycle NaCl from human wastes in a bioregenerative life support system (BLSS). Two halophytic plant species were studied: the salt-accumulating Salicornia europaea and the salt-secreting Limonium gmelinii. During the first two weeks, plants were grown on Knop’s solution, then an average daily amount of urine produced by one human, which had been preliminarily mineralized, was gradually added to the experimental solutions. Nutrient solutions simulating urine mineral composition were gradually added to control solutions. NaCl concentrations in the stock solutions added to the experimental and control solutions were 9 g/L in the first treatment and 20 g/L in the second treatment. The mineralized human urine showed some inhibitory effects on S. europaea and L. gmelinii. The biomass yield of experimental plants was lower than that of control ones. If calculated for the same time period (120 d) and area (1 m²), the amount of sodium chloride taken up by S. europaea plants would be 11.7 times larger than the amount taken up by L. gmelinii plants (486 g/m² vs. 41 g/m²). Thus, S. europaea is the better choice of halophyte for recycling sodium chloride from human wastes in BLSS.