Cave biosignature suites: microbes,minerals, and Mars

Earth's subsurface offers one of the best possible sites to search for microbial life and the characteristic lithologies that life leaves behind. The subterrain may be equally valuable for astrobiology. Where surface conditions are particularly hostile, like on Mars, the subsurface may offer the only habitat for extant lifeforms and access to recognizable biosignatures. We have identified numerous unequivocally biogenic macroscopic, microscopic, and chemical/geochemical cave biosignatures. However, to be especially useful for astrobiology, we are looking for suites of characteristics. Ideally, "biosignature suites" should be both macroscopically and microscopically detectable, independently verifiable by nonmorphological means, and as independent as possible of specific details of life chemistries--demanding (and sometimes conflicting) criteria. Working in fragile, legally protected environments, we developed noninvasive and minimal impact techniques for life and biosignature detection/characterization analogous to Planetary Protection Protocols. Our difficult field conditions have shared limitations common to extraterrestrial robotic and human missions. Thus, the cave/subsurface astrobiology model addresses the most important goals from both scientific and operational points of view. We present details of cave biosignature suites involving manganese and iron oxides, calcite, and sulfur minerals. Suites include morphological fossils, mineral-coated filaments, living microbial mats and preserved biofabrics, 13C and 34S values consistent with microbial metabolism, genetic data, unusual elemental abundances and ratios, and crystallographic mineral forms.     

A Study of the Reflection of Radio Waves from the Plasma Region on the Track behind an Axisymmetric Body Moving in the Earth’s Atmosphere

The results of numerical calculation of the dependences of the electron density, the eigenfrequency and the dielectric plasma permeability on the geometric parameters and the altitude of body motion in the near and far wake behind a thin conical body with a spherical nose blunting have been presented. The electron density maximum has been shown to be located in the region of the neck of the near wake behind the body, which determines the type of this region (supercritical or subcritical). This in turn affects the propagation of radio waves through this plasma region. A comparative analysis was performed for two different bodies with the same ballistic coefficient values. No characteristic distinctions were revealed in the values of electron density or the plasma eigenfrequency in the near and far wake behind these bodies. However, it has been shown that there are differences in the values of the distance from the bottom cross section to the neck of the near wake behind these bodies.     

Interplanetary shocks and sudden impulses during solar maximum (2000) and solar minimum (1995–1996)

In this work a study is performed on the correlation between fast forward interplanetary shock parameters at 1 Astronomical Unit and sudden impulse (SI) amplitudes in the H-component of the geomagnetic field, for periods of solar activity maximum (year 2000) and minimum (year 1995–1996). Solar wind temperature, density and speed, and total magnetic field, were taken to calculate the static pressures (thermal and magnetic) both in the upstream and downstream sides of the shocks. The variations of the solar wind parameters and pressures were then correlated with SI amplitudes. The solar wind speed variations presented good correlations with sudden impulses, with correlation coefficients larger than 0.70 both in solar maximum and solar minimum, whereas the solar wind density presented very low correlation. The parameter better correlated with SI was the square root dynamic pressure variation, showing a larger correlation during solar maximum (r = 0.82) than during solar minimum (r = 0.77). The correlations of SI with square root thermal and magnetic pressure were smaller than with the dynamic pressure, but they also present a good correlation, with r > 0.70 during both solar maximum and minimum. Multiple linear correlation analysis of SI in terms of the three pressure terms have shown that 78% and 85% of the variance in SI during solar maximum and minimum, respectively, are explained by the three pressure variations. Average sudden impulse amplitude was 25 nT during solar maximum and 21 nT during solar minimum, while average square root dynamic pressure variation is 1.20 and 0.86 nPa^1/2 during solar maximum and minimum, respectively. Thus on average, fast forward interplanetary shocks are 33% stronger during solar maximum than during solar minimum, and the magnetospheric SI response has amplitude 20% higher during solar maximum than during solar minimum. A comparison with theoretical predictions (Tsyganenko’s model corrected by Earth’s induced currents) of the coefficient of sudden impulse change with solar wind dynamic pressure variation showed excellent agreement, with values around 17 nT/nPa^1/2.     

Biochemical constraints for survival under Martian conditions.

A wide variety of terrestrial organisms, the so-called "anhydrobiotes," has learned to survive in a state of extreme dehydration in dry environments. Strategies for survival include the accumulation of certain polyols and nonreducing saccharides, which help to prevent damage to membranes and proteins, but at low water partial pressure DNA is also progressively damaged by various lesions, including strand breaks and cross-linking to proteins. These lesions, if they are not too numerous, can be repaired before the first replication step after rehydration, but long-term exposure to dry conditions finally diminishes the chances of survival as these lesions accumulate. If an organism has no chance to repair the accumulated DNA damage during intermittent periods of active life, survival will not exceed a few decades. The restriction of survival by dryness-induced DNA lesions is corroborated by new data on conidia of Aspergillus and the free plasmid pBR 322. Our results will be discussed with respect to the chance of finding dormant life or biochemical fossils on the surface of Mars.     

Imaging the solar corona in the EUV

The SOHO (SOlar and Heliospheric Observatory) satellite was launched on December 2nd 1995. After arriving at the Earth-Sun (L1) Lagrangian point on February 14th 1996, it began to continuously observe the Sun. As one of the instruments onboard SOHO, the EIT (Extreme ultraviolet Imaging Telescope) images the Sun's corona in 4 EUV wavelengths. The He II filter at 304 Å images the chromosphere and the base of the transition region at a temperature of 5 − 8 × 10⁴ K; the Fe IX–X filter at 171 Å images the corona at a temperature of 1.3 × 10⁶ K; the Fe XII filter at 195 Å images the quiet corona outside coronal holes at a temperature of 1.6 × 10⁶ K; and the Fe XV filter at 284 Å images active regions with a temperature of 2.0 × 10⁶ K. About 5000 images have been obtained up to the present. In this paper, we describe also some aspects of the telescope and the detector performance for application in the observations. Images and movies of all the wavelengths allow a look at different phenomena present in the Sun's corona, and in particular, magnetic field reconnection.     

The Impact of Variable Accelerations on Crystal Growth onboard Spacecraft by the Floating Zone Method

The numerical investigation of the impact of time-dependent accelerations (vibrations) on the flow and heat and mass transfer in the melt is carried out for the case of modeling the crystal growth by the floating zone method under conditions of microgravity that exist onboard spacecraft. The effects of the Archimedean buoyancy force and of vibrations of the free surface of fluid are considered separately. When solving the problem of the effect of the vibrations of the free surface of fluid, the previously obtained data were used. It is shown that vibrations of the free surface have a much stronger effect on the processes under consideration than the buoyancy. Some problems that are related to the newly discovered effects are discussed. The use of vibroprotected systems and a rotating magnetic field can help solve these problems. We plan to continue our investigations in future spacecraft experiments, in particular, at the International Space Station, which is under construction at the moment.     

Effect of volatile metabolites of dill, radish and garlic on growth of bacteria

In a model experiment plants were grown in sealed chambers on expanded clay aggregate under the luminance of 150 W/m2 PAR and the temperature of 24 degrees C. Seven bacterial strains under investigation, replicated on nutrient medium surface in Petri dishes, were grown in the atmosphere of cultivated plants. Microbial response was evaluated by the difference between colony size in experiment and in control. In control, bacteria grew in the atmosphere of clean air. To study the effects of volatile metabolites of various plant on microbial growth, the experimental data were compared with the background values defined for each individual experiment. Expanded clay aggregate, luminance, temperature, and sealed chamber (without plants) for the background were the same. Volatile metabolites from 28-days old radish plants have been reliably established to have no effect on the growth of microbes under investigation. Metabolites of 30-days old dill and 50-days old garlic have been established to have reliable bacteriostatic effect on the growth of three bacterial strains. Dill and garlic have been found to have different range of effects of volatile substances on bacterial growth. Volatile metabolites of dill and garlic differed in their effect on the sensitivity spectrum of bacteria. An attempt has been made to describe the obtained data mathematically.