Entrapment of bacteria in fluid inclusions in laboratory-grown halite

Cells of the bacterium Pseudomonas aeruginosa, which were genetically modified to produce green fluorescent protein, were entrapped in fluid inclusions in laboratory-grown halite. The bacteria were used to inoculate NaCl-saturated aqueous solutions, which were allowed to evaporate and precipitate halite. The number, size, and distribution of fluid inclusions were highly variable, but did not appear to be affected by the presence of the bacteria. Many of the inclusions in crystals from inoculated solutions contained cells in populations ranging from two to 20. Microbial attachment to crystal surfaces was neither evident nor necessary for entrapment. Cells occurred exclusively within fluid inclusions and were not present in the crystal matrix. In both the inclusions and the hypersaline solution, the cells fluoresced and twitched, which indicates that the bacteria might have remained viable after entrapment. The fluorescence continued up to 13 months after entrapment, which indicates that little degradation of the bacteria occurred over that time interval. The entrapment, fluorescence, and preservation of cells were independent of the volume of hypersaline solution used or whether the solutions were completely evaporated prior to crystal extraction. The results of this study have a wide range of implications for the long-term survival of microorganisms in fluid inclusions and their detection through petrography. The results also demonstrate the preservation potential for microbes in hypersaline fluid inclusions, which could allow cells to survive harsh conditions of space, the deep geologic past, or burial in sedimentary basins.     

Doppler characteristics of HF spread Doppler clutter recorded at Alice Springs,Australia

Mean night-time peak power, Doppler shift and Doppler width of spread Doppler clutter (SDC) received by a high frequency backscatter radar located at Alice Springs, Australia from 2000 to 2018 is presented as a function of azimuth, sunspot number, time of year and frequency. The sampled region covers 90 degrees from West to North and includes the northern and southern equatorial anomalies.SDC peak power diminished across all azimuths during the winter solstice from around May to August (local winter) coinciding with the global decrease in F layer density due to the annual non-seasonal F2 anomaly but was generally constant during the equinoxes. In contrast, SDC Doppler width and inbound Doppler shift both increased during the equinoxes and exhibited azimuthal dependence related to the eastward equatorial plasma drift.SDC peak power increased with increasing sunspot number with frequency dependence during winter but not summer. Inbound Doppler shift and Doppler width increased with increasing sunspot number during equinox but not solstice with a strong dependence on azimuth and a weak dependence on frequency.     

A novel satellite image encryption algorithm based on hyperchaotic systems and Josephus problem

With the rapid growth of the number of Earth observation satellite (EOS) supporting critical applications, it is required to improve the security techniques to protect the sensitive data and images during the transmission between the satellites and the ground stations. This paper introduces a new satellite image encryption algorithm based on the Linear Feedback Shift Register (LFSR) generator, SHA 512 hash function, hyperchaotic systems, and Josephus problem. LFSR generates a matrix that is used to construct the 512-bits value of the hash function. These bits are used to set the initial values and parameters of the proposed encryption algorithm. Firstly, the six dimensions (6-D) hyperchaotic system is divided into three parts, where every two equations are considered as one part. Secondly, the 1-D hyperchaotic logistic-tent system is considered as the controller to select one part. The selected part is used to generate a matrix that is XORed with the original image. Thirdly, the scrambling operation by Josephus sequences is applied to the output of the previous step by scrambling the rows and the columns according to the selected part to produce the pre-encrypted image. Finally, if the number of iterations is less than the required number which is considered as a parameter of the secret key, the previous operations will be repeated in the pre-encrypted image; otherwise, the pre-encrypted image is considered as the final cipher image. Experimental and analyses results show that the proposed algorithm has good performance in terms of high level of security, large enough key-space, tolerance to Single Event Upsets (SEU) as well as low time complexity.     

Equatorial anomaly according to the Interkosmos-19 data and IRI model: A comparison

The comparison of the IRI model with the foF2 distribution in the equatorial anomaly region obtained by topside sounding onboard the Interkosmos-19 satellite has been carried out. The global distribution of foF2 in terms of LT-maps was constructed by averaging Intercosmos-19 data for summer, winter, and equinox. These maps, in fact, represent an empirical model of the equatorial anomaly for high solar activity F10.7 ~ 200. The comparison is carried out for the latitudinal foF2 profiles in the characteristic longitudinal sectors of 30, 90, 210, 270, and 330°, as well as for the longitudinal variations in foF2 over the equator. The largest difference between the models (up to 60%) for any season was found in the Pacific longitudinal sector of 210°, where there are a few ground-based sounding stations. Considerable discrepancies, however, are sometimes observed in the longitudinal sectors, where there are many ground-based stations, for example, in the European or Indian sector. The discrepancies reach their maximum at 00 LT, since a decay of the equatorial anomaly begins before midnight in the IRI model and after midnight according to the Interkosmos-19 data. The discrepancies are also large in the morning at 06 LT, since in the IRI model, the foF2 growth begins long before sunrise. In the longitudinal variations in foF2 over the equator at noon, according to the satellite data, four harmonics are distinguished in the June solstice and at the equinox, and three harmonics in the December solstice, while in the IRI model only two and one harmonics respectively are revealed. In diurnal variations in foF2 and, accordingly, in the equatorial anomaly intensity, the IRI model does not adequately reproduce even the main, evening extremum.     

Space Station habitability research.

The purpose and scope of the Habitability Research Group within the Space Human Factors Office at the NASA/Ames Research Center is described. Both near-term and long-term research objectives in the space human factors program pertaining to the U.S. manned Space Station are introduced. The concept of habitability and its relevancy to the U.S. space program is defined within a historical context. The relationship of habitability research to the optimization of environmental and operational determinants of productivity is discussed. Ongoing habitability research efforts pertaining to living and working on the Space Station are described.     

Autoignition of fuel-oxidizer mixtures in microgravity

The paper examines the limitations of quasi-steady autoignition theory in providing information needed for the characterization of autoignition temperature fields. Time-dependent autoignition theory is then utilized to examine the autoignition behavior of a reactive system where both wall catalysis and gas phase kinetic rates are significant. It is found that a diverse range of space-time temperature-composition-pressure histories is possible, depending on the system's thermo-kinetic characteristics.     

Effects of microgravity on c-fos gene expression in osteoblast-like MC3T3-E1 cells.

The paper summarizes the data on proliferation and gravity-related gene expression of osteoblasts that were obtained from an experiment conducted under simulated and real microgravity conditions. Simulated microgravity conditions obtained in a clinostat depress proliferation of both osteoblast-like MC3T3-E1 and HeLa carcinoma cells. This depression of proliferation occurs in a collagen gel culture in which the flow of culture medium by rotation may be reduced. Interestingly, MC3T3-E1 cells which are probably one of target cells to microgravity are more sensitive than the HeLa cells. Simulated microgravity inhibited the epidermal growth factor (EGF)-induced c-fos gene expression in the MC3T3-El cells. To examine in detail the effect of real microgravity on the EGF signal transduction cascade in osteoblasts, MC3T3-E1 cells were cultured in the Cell Culture Experiment Module of the sounding rocket TR-1A6. The EGF-induced c-fos expression in cells was depressed under short-term microgravity conditions in the sounding rocket, while the phosphorylation of mitogen-activated protein kinase (MAPK) was not affected compared with the controls grown on the ground. These results suggest that an action site of microgravity in the signal transduction pathway may be downstream of MAPK.     

A concept of increasing efficiency for ramjet operation

The vortical mixing concept in the ramjet channel is analyzed. The results of simulating the gasodynamic scheme permit the concept of increasing efficiency for ramjet operation to be substantiated.     

Technique of integral diagnostics for a radio-frequency inductively coupled plasma discharge unit of an RF ion thruster

A technique of integral diagnostics for an RF inductively coupled plasma gas discharge unit of an ion thruster is proposed. This technique includes a priori measurements of antenna coil electrical parameters in free space and in assembled state as well as experimental determination of the antenna coil currents with and without discharge.