An examination of the carbon isotope effects associated with amino acid biosynthesis

Stable carbon isotope ratios (delta(13)C) were determined for alanine, proline, phenylalanine, valine, leucine, isoleucine, aspartate (aspartic acid and asparagine), glutamate (glutamic acid and glutamine), lysine, serine, glycine, and threonine from metabolically diverse microorganisms. The microorganisms examined included fermenting bacteria, organotrophic, chemolithotrophic, phototrophic, methylotrophic, methanogenic, acetogenic, acetotrophic, and naturally occurring cryptoendolithic communities from the Dry Valleys of Antarctica. Here we demonstrated that reactions involved in amino acid biosynthesis can be used to distinguish amino acids formed by life from those formed by nonbiological processes. The unique patterns of delta(13)C imprinted by life on amino acids produced a biological bias. We also showed that, by applying discriminant function analysis to the delta(13)C value of a pool of amino acids formed by biological activity, it was possible to identify key aspects of intermediary carbon metabolism in the microbial world. In fact, microorganisms examined in this study could be placed within one of three metabolic groups: (1) heterotrophs that grow by oxidizing compounds containing three or more carbon-to-carbon bonds (fermenters and organotrophs), (2) autotrophs that grow by taking up carbon dioxide (chemolitotrophs and phototrophs), and (3) acetoclastic microbes that grow by assimilation of formaldehyde or acetate (methylotrophs, methanogens, acetogens, and acetotrophs). Furthermore, we demonstrated that cryptoendolithic communities from Antarctica grouped most closely with the autotrophs, which indicates that the dominant metabolic pathways in these communities are likely those utilized for CO(2 )fixation. We propose that this technique can be used to determine the dominant metabolic types in a community and reveal the overall flow of carbon in a complex ecosystem.     

Superlow-Frequency MHD-Eigenoscillations With a Discrete Spectrum in Parabolic Model of Magnetosphere

A new concept of the global magnetospheric resonator is suggested for fast magnetosonic waves in which the role of the resonator is played by the near part of the plasma sheet. It is shown that the magnetosonic wave is confined in this region of the magnetosphere throughout its boundaries. The representative value of the resonator's eigenfrequency estimated at f ≈ 1 mHz is in good agreement with observational data on ultraThe theory explains the ground-based localization of the oscillations observed in the midnight-morning sector of the high-latitude magnetosphere.      

A Mechanism of Solar Variability Effect on Radiative Balance of the Earth Atmosphere

Possible mechanisms of solar-climatic connections, which may be of importance as over short and long time intervals, are discussed. The variations of energetic balance of Earth's climatic system for the last fifty years are estimated. It is ascertained that the disbalance between the flux of solar energy that comes to the Earth and radiates to space is of 0.1% for the last ten years. The suggested mechanism makes it possible to explain not only the observed variation of the enthalpy of the Earth's climatic system for the period 1910-1980, but also the climate anomalies during last thousand years: the climate optimum in 12 century, and"small glacial period" in 16-17 centuries.      

一种利用功率反演和线性约束最小方差算法的自适应天线

介绍了一种新的基于功率反演和线性约束最小方差的算法,以高度抑制GPS接收机的干扰信号。这种结构通过提调整天线阵列的权值,实时地接收并改变来自各方向的GPS信号,同时对不同方向的干扰信号有高的抑制比。对固定和移动的干扰都做了仿真,仿真表明这种结构有很深的零点,对固定干扰信号的抑制比可达到115dB,对移动干扰信号的抑制比可达到94dB。     

UMR: A utility-maximizing routing algorithm for delay-sensitive service in LEO satellite networks

This paper develops a routing algorithm for delay-sensitive packet transmission in a low earth orbit multi-hop satellite network consists of micro-satellites. The micro-satellite low earth orbit(MS-LEO) network endures unstable link connection and frequent link congestion due to the uneven user distribution and the link capacity variations. The proposed routing algorithm,referred to as the utility maximizing routing(UMR) algorithm, improve the network utility of the MS-LEO network for carrying flows with strict end-to-end delay bound requirement. In UMR, first, a link state parameter is defined to capture the link reliability on continuing to keep the end-to-end delay into constraint; then, on the basis of this parameter, a routing metric is formulated and a routing scheme is designed for balancing the reliability in delay bound guarantee among paths and building a path maximizing the network utility expectation. While the UMR algorithm has many advantages, it may result in a higher blocking rate of new calls. This phenomenon is discussed and a weight factor is introduced into UMR to provide a flexible performance option for network operator. A set of simulations are conducted to verify the good performance of UMR, in terms of balancing the traffic distribution on inter-satellite links, reducing the flow interruption rate,and improving the network utility.     

SOLID3: a multiplex antibody microarray-based optical sensor instrument for in situ life detection in planetary exploration

The search for unequivocal signs of life on other planetary bodies is one of the major challenges for astrobiology. The failure to detect organic molecules on the surface of Mars by measuring volatile compounds after sample heating, together with the new knowledge of martian soil chemistry, has prompted the astrobiological community to develop new methods and technologies. Based on protein microarray technology, we have designed and built a series of instruments called SOLID (for "Signs Of LIfe Detector") for automatic in situ detection and identification of substances or analytes from liquid and solid samples (soil, sediments, or powder). Here, we present the SOLID3 instrument, which is able to perform both sandwich and competitive immunoassays and consists of two separate functional units: a Sample Preparation Unit (SPU) for 10 different extractions by ultrasonication and a Sample Analysis Unit (SAU) for fluorescent immunoassays. The SAU consists of five different flow cells, with an antibody microarray in each one (2000 spots). It is also equipped with an exclusive optical package and a charge-coupled device (CCD) for fluorescent detection. We demonstrated the performance of SOLID3 in the detection of a broad range of molecular-sized compounds, which range from peptides and proteins to whole cells and spores, with sensitivities at 1-2?ppb (ng?mL?1) for biomolecules and 10? to 103 spores per milliliter. We report its application in the detection of acidophilic microorganisms in the Río Tinto Mars analogue and report the absence of substantial negative effects on the immunoassay in the presence of 50?mM perchlorate (20 times higher than that found at the Phoenix landing site). Our SOLID instrument concept is an excellent option with which to detect biomolecules because it avoids the high-temperature treatments that may destroy organic matter in the presence of martian oxidants.     

A two-tiered approach to assessing the habitability of exoplanets

In the next few years, the number of catalogued exoplanets will be counted in the thousands. This will vastly expand the number of potentially habitable worlds and lead to a systematic assessment of their astrobiological potential. Here, we suggest a two-tiered classification scheme of exoplanet habitability. The first tier consists of an Earth Similarity Index (ESI), which allows worlds to be screened with regard to their similarity to Earth, the only known inhabited planet at this time. The ESI is based on data available or potentially available for most exoplanets such as mass, radius, and temperature. For the second tier of the classification scheme we propose a Planetary Habitability Index (PHI) based on the presence of a stable substrate, available energy, appropriate chemistry, and the potential for holding a liquid solvent. The PHI has been designed to minimize the biased search for life as we know it and to take into account life that might exist under more exotic conditions. As such, the PHI requires more detailed knowledge than is available for any exoplanet at this time. However, future missions such as the Terrestrial Planet Finder will collect this information and advance the PHI. Both indices are formulated in a way that enables their values to be updated as technology and our knowledge about habitable planets, moons, and life advances. Applying the proposed metrics to bodies within our Solar System for comparison reveals two planets in the Gliese 581 system, GJ 581 c and d, with an ESI comparable to that of Mars and a PHI between that of Europa and Enceladus.