Manipulating light and temperature to minimize environmental stress in the plant component of bioregenerative life support systems.

Our experiments examined enhancing tolerance of the photosynthesizing component to possible deviations in thermal or illumination conditions inside a bioregenerative life support system (BLSS). In the event of one parameter getting beyond its optimum, the values of other parameters may ensure minimal damage to the plant component during the period of environmental stress. With wheat plants (one of key elements of the plant component) as an example the work considers whether it is possible to enhance thermal tolerance by varying light intensity. Increase of air temperature to 35 degrees C or 45 degrees C with light intensity of 60 W/m2 PAR has been shown to substantially inhibit the photosynthesis processes; at 150 W/m2 PAR photosynthesis decreases from 50% to 100%, respectively; when light intensity is increased to 240 W/m2 PAR photosynthesis increased more than 70% at 35 degrees C and decreased at 45 degrees C by only 20%. Thus, light intensity can be increased to avoid or decrease the inhibiting effect of high temperatures. On the other hand, tolerance of wheat plants to prolonged absence of light can be substantially enhanced by decreasing during this period air temperature to temperatures close to 0 degrees C.     

Doppler imaging based on radar target precession

A unique form of target motion reminiscent of the precession of a rotating top is investigated. Images are generated with the radar looking either along the precession axis or orthogonally to it. Features of the two methods are discussed and demonstrated by imaging simulated point target scenes     

The heliosphere

The history and current status of heliospheric physics are outlined very briefly. It is emphasized that the existing Voyager Interstellar Mission should go a long way to answering many of the outstanding questions during the next one or two decades, especially those concerning the nature and structure of the solar wind termination shock.     

Evolution of the Martian water cycle.

The current Martian water cycle is extremely asymmetric, with large amounts of vapor subliming off a permanent north polar water ice cap in northern summer, but with no apparent major source of water vapor in the southern hemisphere. Detailed simulations of this process with a three-dimensional circulation model indicate that the summertime interhemispheric exchange (Hadley cell) is very much stronger than transport by eddies in other seasons. As a result, water ice would be distributed globally were it not for the buffering action of regolith soil adsorption which limits the net flux of water vapor off the north polar cap to amounts that are insignificant even on the scale of thousands of years. It has been suggested that the polar layered deposits are the result of exchange on these long time scales, driven by changes in Martian orbital parameters. We therefore are conducting simulations to test the effect of varied orbital parameters on the Martian water cycle. We find that when the perihelion summer pole is charged with a polar water ice cap, large quantities of water are quickly transfered to the aphelion summer pole, setting up an annual cycle that resembles the present one. Thus, the adsorptivity of the Martian regolith may be in the narrow range where it can limit net transport from the aphelion but not the perihelion pole.     

Spaceflight and clinorotation cause cytoskeleton and mitochondria changes and increases in apoptosis in cultured cells.

The cytoskeleton is a complex network of fibers that is sensitive to environmental factors including microgravity and altered gravitational forces. Cellular functions such as transport of cell organelles depend on cytoskeletal integrity; regulation of cytoskeletal activity plays a role in cell maintenance, cell division, and apoptosis. Here we report cytoskeletal and mitochondria alterations in cultured human lymphocyte (Jurkat) cells after exposure to spaceflight and in insect cells of Drosophila melanogaster (Schneider S-1) after exposure to conditions created by clinostat rotation. Jurkat cells were flown on the space shuttle in Biorack cassettes while Schneider S-1 cells were exposed to altered gravity forces as produced by clinostat rotation. The effects of both treatments were similar in the different cell types. Fifty percent of cells displayed effects on the microtubule network in both cell lines. Under these experimental conditions mitochondria clustering and morphological alterations of mitochondrial cristae was observed to various degrees after 4 and 48 hours of culture. Jurkat cells underwent cell divisions during exposure to spaceflight but a large number of apoptotic cells was also observed. Similar results were obtained in Schneider S-1 cells cultured under clinostat rotation. Both cell lines displayed mitochondria abnormalities and mitochondria clustering toward one side of the cells which is interpreted to be the result of microtubule disruption and failure of mitochondria transport along microtubules. The number of mitochondria was increased in cells exposed to altered gravity while cristae morphology was severely affected indicating altered mitochondria function. These results show that spaceflight as well as altered gravity produced by clinostat rotation affects microtubule and mitochondria organization and results in increases in apoptosis. Grant numbers: NAG 10-0224, NAG2-985.     

An Electric Shock Model for Experiments on Critical Ionization Velocity

To describe the generation of the electric field by a discontinuity of the Hall current, an equation of the third order was obtained by using the electric charge conservation and Ohm's laws. Solutions to this equation are used to model the electric impulses detected in experiments aimed to verify the Alfven hypothesis on the critical ionization velocity in the process of the collision of neutral gas with magnetized plasma. A quantitative agreement with the experiment was attained, and the basic features of the measured signals were modeled under an assumption of strong anomalous resistance behind the discontinuity. Apparently, such an anomalous resistance occurs due to trapping of the current carriers by a small-scale modulation of the electric field.     

Ballooning perturbations in the inner magnetosphere of the Earth: Spectrum,stability and eigenmode analysis

We investigate the generation of ballooning perturbations in the inner magnetosphere of the Earth in the dipole model of the geomagnetic field taking into account ionospheric boundary conditions. The ionosphere is considered as a thin layer with finite conductivity. The eigenmode spectrum is discrete and consists of Alfvén, slow magnetosonic, flute and incompressible modes. Their interaction depends on ionospheric conductivity. The decay rate is small in noon and night sectors and large in dawn and dusk sectors. The lowest stability threshold α/γ ≈ 4.25 is determined by flute modes.     

Calibration techniques for magnetometers implementing on-board de-spinning algorithms

The Fluxgate Magnetometer experiments on-board the European Space Agency’s four spacecraft Cluster Mission have the capability to store sampled magnetic field vectors in the instrument memory, either as a full resolution ‘event capture’ or as spin-resolution vectors transformed into a non-spinning co-ordinate system (de-spun). The latter capability has ensured a dataset is available which extends the partial orbital coverage achieved during nominal operations in the first years of operation. The on-board de-spin is achieved using a Walsh function with Haar coefficients and allows for up to 27 h additional data per non-coverage interval. A number of commissioning orbits were used to verify the accuracy of data collected by the de-spin mode, whereby individual spacecraft were operated separately in a number of standard normal sampling and de-spin mode combinations. Up to the present time, this data has not been available to the Cluster community. We present results here comparing the performance of the on-board de-spin algorithm versus the normal sampling modes over a number of boundary layer crossings, describe the techniques used for calibration and timeline recovery, and outline the context in which the data may be usable in future studies.     

Results from the ESA SREM monitors and comparison with existing radiation belt models

The Standard Radiation Environment Monitor (SREM) is a simple particle detector developed for wide application on ESA satellites. It measures high-energy protons and electrons of the space environment with a 20° angular resolution and limited spectral information. Of the ten SREMs that have been manufactured, four have so far flown. The first model on STRV-1c functioned well until an early spacecraft failure. The other three are on-board, the ESA spacecraft INTEGRAL, ROSETTA and PROBA-1. Another model is flying on GIOVE-B, launched in April 2008 with three L-2 science missions to follow: both Herschel and Planck in 2008, and GAIA in 2011). The diverse orbits of these spacecraft and the common calibration of the monitors provides a unique dataset covering a wide range of B-L* space, providing a direct comparison of the radiation levels in the belts at different locations, and the effects of geomagnetic shielding. Data from the PROBA/SREM and INTEGRAL/IREM are compared with existing radiation belt models.     

Light microscopic analysis of the gravireceptor in Xenopus larvae developed in hypogravity.

The paper describes an investigation of the influence of gravity on the early differentiation of gravity receptors in Xenopus embryos and larvae. There is evidence that the expression of crystals in the saccus endolymphaticus was statistically greater when the embryos developed in near weightlessness (hypogravity) than on earth. The function of these crystals is unknown but they may contribute to the functioning of the vestibular apparatus.