Soybean cultivar selection for Bioregenerative Life Support Systems (BLSSs) – Hydroponic cultivation

Four soybean cultivars (‘Atlantic’, ‘Cresir’, ‘Pr91m10’ and ‘Regir’), selected through a theoretical procedure as suitable for cultivation in BLSS, were evaluated in terms of growth and production. Germination percentage and Mean Germination Time (MGT) were measured. Plants were cultivated in a growth chamber equipped with a recirculating hydroponic system (Nutrient Film Technique). Cultivation was performed under controlled environmental conditions (12 h photoperiod, light intensity 350 μmol m⁻² s⁻¹, temperature regime 26/20 °C light/dark, relative humidity 65–75%). Fertigation was performed with a standard Hoagland solution, modified for soybean specific requirements, and EC and pH were kept at 2.0 dS m⁻¹ and 5.5 respectively.     

Low ionization lines in high luminosity quasars: The calcium triplet

In order to investigate where and how low ionization lines are emitted in quasars we are studying a new collection of spectra of the CaII triplet at λ8498, λ8542, λ8662 observed with the Very Large Telescope (VLT) using the Infrared Spectrometer And Array Camera (ISAAC). Our sample involves luminous quasars at intermediate redshift for which CaII observations are almost nonexistent. We fit the CaII triplet and the OI λ8446 line using the Hβ profile as a model. We derive constraints on the line emitting region from the relative strength of the CaII triplet, OI λ8446 and Hβ.     

Detectability of planetary characteristics in disk-averaged spectra II: synthetic spectra and light-curves of earth

Spatially and spectrally resolved models were used to explore the observational sensitivity to changes in atmospheric and surface properties and the detectability of surface biosignatures in the globally averaged spectra and light-curves of the Earth. Compared with previous efforts to characterize the Earth using disk-averaged models, a more comprehensive and realistic treatment of the surface and atmosphere was taken into account here. Our results are presented as a function of viewing geometry and phases at both visible/near-infrared (0.5-1.7 microm) and mid-infrared (5-25 microm) wavelength ranges, applicable to the proposed NASA-Terrestrial Planet Finder visible coronagraph and mid-infrared interferometer and to the ESADarwin mission architectures. Clouds can change the thermal emission by as much as 50% compared with the cloud-free case and increase the visible albedo by up to 500% for completely overcast cases at the dichotomy phase. Depending on the observed phase and their distribution and type, clouds can also significantly alter the spectral shape. Moreover, clouds impact the detectability of surface biosignatures in the visible wavelength range. Modeling the disk-averaged sensitivity to the "red-edge," a distinctive spectral signature of vegetation, showed that Earth's land vegetation could be seen in disk-averaged spectra, even with cloud cover, when the signal was averaged over the daily time scale. We found that vegetation is more readily discriminated from clouds at dichotomy (50% illumination) rather than at full phase. The detectability of phytoplankton was also explored, but was found to be more difficult to detect in the disk-average than land vegetation.     

Origin of informational polymers and the search for non-terran life: protection of the polymeric state of DNA by phosphate minerals

An in-depth analysis of the effects exerted on the DNA backbone by 25 crystal phosphate minerals is reported. Degradation of DNA oligomers was performed with two different reactions: Hydrolysis following Nucleophilic Degradation (HND), initiated by the nucleophilic addition of formamide on both purine and pyrimidine nucleobases, and Hydrolysis following Nucleophilic Substitution (HNS) carried on by water and starting with the removal of a nondegraded base. A complete panel of effects on the phosphoester bonds, from protection to enhanced instability to absence of interference, is described. These effects differ in the different degradation pathways and in different physical-chemical conditions. The relationship between the hardness of the mineral and its protective ability is discussed. In addition to its interest per se, this study was prompted by the observed catalytic abilities of soluble and mineral phosphates (Saladino et al., 2006c) on the synthetic reactions by formamide. The relevance of these observations in the search for nonterran life is discussed.     

Biosignatures from Earth-like planets around M dwarfs

Coupled one-dimensional photochemical-climate calculations have been performed for hypothetical Earth-like planets around M dwarfs. Visible/near-infrared and thermal-infrared synthetic spectra of these planets were generated to determine which biosignature gases might be observed by a future, space-based telescope. Our star sample included two observed active M dwarfs-AD Leo and GJ 643-and three quiescent model stars. The spectral distribution of these stars in the ultraviolet generates a different photochemistry on these planets. As a result, the biogenic gases CH4, N2O, and CH3Cl have substantially longer lifetimes and higher mixing ratios than on Earth, making them potentially observable by space-based telescopes. On the active M-star planets, an ozone layer similar to Earth's was developed that resulted in a spectroscopic signature comparable to the terrestrial one. The simultaneous detection of O2 (or O3) and a reduced gas in a planet's atmosphere has been suggested as strong evidence for life. Planets circling M stars may be good locations to search for such evidence.     

Detectability of planetary characteristics in disk-averaged spectra. I: The Earth model

Over the next 2 decades, NASA and ESA are planning a series of space-based observatories to detect and characterize extrasolar planets. This first generation of observatories will not be able to spatially resolve the terrestrial planets detected. Instead, these planets will be characterized by disk-averaged spectroscopy. To assess the detectability of planetary characteristics in disk-averaged spectra, we have developed a spatially and spectrally resolved model of the Earth. This model uses atmospheric and surface properties from existing observations and modeling studies as input, and generates spatially resolved high-resolution synthetic spectra using the Spectral Mapping Atmospheric Radiative Transfer model. Synthetic spectra were generated for a variety of conditions, including cloud coverage, illumination fraction, and viewing angle geometry, over a wavelength range extending from the ultraviolet to the farinfrared. Here we describe the model and validate it against disk-averaged visible to infrared observations of the Earth taken by the Mars Global Surveyor Thermal Emission Spectrometer, the ESA Mars Express Omega instrument, and ground-based observations of earthshine reflected from the unilluminated portion of the Moon. The comparison between the data and model indicates that several atmospheric species can be identified in disk-averaged Earth spectra, and potentially detected depending on the wavelength range and resolving power of the instrument. At visible wavelengths (0.4-0.9 microm) O3, H2O, O2, and oxygen dimer [(O2)2] are clearly apparent. In the mid-infrared (5-20 microm) CO2, O3, and H2O are present. CH4, N2O, CO2, O3, and H2O are visible in the near-infrared (1-5 microm). A comprehensive three-dimensional model of the Earth is needed to produce a good fit with the observations.     

Disk-averaged synthetic spectra of Mars

The principal goal of the NASA Terrestrial Planet Finder (TPF) and European Space Agency's Darwin mission concepts is to directly detect and characterize extrasolar terrestrial (Earthsized) planets. This first generation of instruments is expected to provide disk-averaged spectra with modest spectral resolution and signal-to-noise. Here we use a spatially and spectrally resolved model of a Mars-like planet to study the detectability of a planet's surface and atmospheric properties from disk-averaged spectra. We explore the detectability as a function of spectral resolution and wavelength range, for both the proposed visible coronograph (TPFC) and mid-infrared interferometer (TPF-I/Darwin) architectures. At the core of our model is a spectrum-resolving (line-by-line) atmospheric/surface radiative transfer model. This model uses observational data as input to generate a database of spatially resolved synthetic spectra for a range of illumination conditions and viewing geometries. The model was validated against spectra recorded by the Mars Global Surveyor-Thermal Emission Spectrometer and the Mariner 9-Infrared Interferometer Spectrometer. Results presented here include disk-averaged synthetic spectra, light curves, and the spectral variability at visible and mid-infrared wavelengths for Mars as a function of viewing angle, illumination, and season. We also considered the differences in the spectral appearance of an increasingly ice-covered Mars, as a function of spectral resolution, signal-to-noise and integration time for both TPF-C and TPFI/ Darwin.     

Spectral signatures of photosynthesis. II. Coevolution with other stars and the atmosphere on extrasolar worlds

As photosynthesis on Earth produces the primary signatures of life that can be detected astronomically at the global scale, a strong focus of the search for extrasolar life will be photosynthesis, particularly photosynthesis that has evolved with a different parent star. We take previously simulated planetary atmospheric compositions for Earth-like planets around observed F2V and K2V, modeled M1V and M5V stars, and around the active M4.5V star AD Leo; our scenarios use Earth's atmospheric composition as well as very low O2 content in case anoxygenic photosynthesis dominates. With a line-by-line radiative transfer model, we calculate the incident spectral photon flux densities at the surface of the planet and under water. We identify bands of available photosynthetically relevant radiation and find that photosynthetic pigments on planets around F2V stars may peak in absorbance in the blue, K2V in the red-orange, and M stars in the near-infrared, in bands at 0.93-1.1 microm, 1.1-1.4 microm, 1.5-1.8 microm, and 1.8-2.5 microm. However, underwater organisms will be restricted to wavelengths shorter than 1.4 microm and more likely below 1.1 microm. M star planets without oxygenic photosynthesis will have photon fluxes above 1.6 microm curtailed by methane. Longer-wavelength, multi-photo-system series would reduce the quantum yield but could allow for oxygenic photosystems at longer wavelengths. A wavelength of 1.1 microm is a possible upper cutoff for electronic transitions versus only vibrational energy; however, this cutoff is not strict, since such energetics depend on molecular configuration. M star planets could be a half to a tenth as productive as Earth in the visible, but exceed Earth if useful photons extend to 1.1 microm for anoxygenic photosynthesis. Under water, organisms would still be able to survive ultraviolet flares from young M stars and acquire adequate light for growth.     

Tomographic reconstruction of wet and total refractivity fields from GNSS receiver networks

One of the most attractive scientific issues in the use of GNSS (Global Navigation Satellite System) signals, from a meteorological point of view, is the retrieval of high resolution tropospheric water vapour maps. The real-time (or quasi real-time) knowledge of such distributions could be very useful for several applications, from operative meteorology to atmospheric modelling. Moreover, the exploitation of wet refractivity field reconstruction techniques can be used for atmospheric delay compensation purposes and, as a very promising activity, it could be applied for example to calibrate SAR or Interferometric-SAR (In-SAR) observations for land remote sensing. This is in fact one of the objectives of the European Space Agency project METAWAVE (Mitigation of Electromagnetic Transmission errors induced by Atmospheric Water vapour Effects), in which several techniques are investigated and results were compared to identify a strategy to remove the contribution of water vapour induced propagation delays in In-SAR products. Within this project, the tomographic reconstruction of three dimensional wet refractivity fields from tropospheric delays observed by a local GNSS network (9 dual frequency GPS receivers) deployed over Como area (Italy), during 12–18 October, 2008, was performed. Despite limitations due to the network design, internal consistency tests prove the efficiency of the adopted tomographic approach: the rms of the difference between reconstructed and GNSS observed Zenith Wet Delays (ZWD) are in the order of 4 mm. A good agreement is also observed between our ZWDs and corresponding delays obtained by vertically integrating independent wet refractivity fields, taken by co-located meteorological analysis. Finally, during the observing period, reconstructed vertical wet refractivity profiles evolution reveals water vapour variations induced by simple cloud covering. Even if our main goal was to demonstrate the effectiveness in adopting tomographic reconstruction procedures for the evaluation of propagation delays inside water vapour fields, the actual water vapour vertical variability and its evolution with time is well reproduced, demonstrating also the effectiveness of the inferred 3D wet refractivity fields.     

Soybean cultivar selection for Bioregenerative Life Support Systems (BLSS) – Theoretical selection

The development of plant-based Bioregenerative Life Support Systems (BLSS) is a requirement for the realization of long-duration exploratory-class manned missions in so far as they help fulfilling astronauts’ needs including nutritional demands, air regeneration and psychological support.