Testing interplanetary transfer of bacteria between Earth and Mars as a result of natural impact phenomena and human spaceflight activities

Interplanetary transport of microbes between Earth and Mars can be envisioned to occur either naturally as a consequence of impacts (lithopanspermia) or as a result of human and robotic spaceflight. In either case, the considerations for modeling successful transfer of microbial life are similar. The probability of microbes surviving either natural or human-mediated transfer is a function of: the initial population size and composition (i.e., the bioload); survival of launch, transit through space, entry and deposition; and ability to survive and proliferate on the recipient planet. Modeling this process for testing lithopanspermia and for mitigation of forward and back contamination for planetary protection purposes calls for accurate simulation of all aspects of transfer.     

Resistance of bacterial endospores to outer space for planetary protection purposes--experiment PROTECT of the EXPOSE-E mission

Spore-forming bacteria are of particular concern in the context of planetary protection because their tough endospores may withstand certain sterilization procedures as well as the harsh environments of outer space or planetary surfaces. To test their hardiness on a hypothetical mission to Mars, spores of Bacillus subtilis 168 and Bacillus pumilus SAFR-032 were exposed for 1.5 years to selected parameters of space in the experiment PROTECT during the EXPOSE-E mission on board the International Space Station. Mounted as dry layers on spacecraft-qualified aluminum coupons, the "trip to Mars" spores experienced space vacuum, cosmic and extraterrestrial solar radiation, and temperature fluctuations, whereas the "stay on Mars" spores were subjected to a simulated martian environment that included atmospheric pressure and composition, and UV and cosmic radiation. The survival of spores from both assays was determined after retrieval. It was clearly shown that solar extraterrestrial UV radiation (λ≥110?nm) as well as the martian UV spectrum (λ≥200?nm) was the most deleterious factor applied; in some samples only a few survivors were recovered from spores exposed in monolayers. Spores in multilayers survived better by several orders of magnitude. All other environmental parameters encountered by the "trip to Mars" or "stay on Mars" spores did little harm to the spores, which showed about 50% survival or more. The data demonstrate the high chance of survival of spores on a Mars mission, if protected against solar irradiation. These results will have implications for planetary protection considerations.     

Planetary protection—A microbial ethics approach

Planetary protection policies designed to reduce the cross-transfer of life on spacecraft from one planet to another can either be formulated from the pragmatic instrumental needs of scientific exploration, or from ethical principles. I address planetary protection concerns by starting from a normative ethical framework for the treatment of microorganisms. This argues that they have intrinsic value at the level of the individual through to the level of the community, but at the individual level this ethic can only be theoretical. This approach yields a solution to the problem of the inevitable contamination of Mars by human explorers and suggests that in some instances the local contamination of other planets may be acceptable. An exception would be where this contamination would cause destruction of microbial ecosystems. Within the framework of such an ethic, the term ‘planetary protection’ may be normatively too narrow and ‘planetary preservation’ may better describe the activity of controlling cross-inoculation of planets. I discuss an example of a contamination event that might be ethically acceptable within the framework of ‘preservation’, but would be regarded as unacceptable under current planetary ‘protection’ guidelines.     

Assessing the plausibility of life on other worlds

As the field of astrobiology matures and search strategies for life on other worlds are developed, the need to analyze in a systematic way the plausibility for life on other planetary systems becomes increasingly apparent. We propose the adoption of a simple plausibility of life (POL) rating system based on specific criteria. Category I applies to any body shown to have conditions essentially equivalent to those on Earth. Category II applies to bodies for which there is evidence of liquid water and sources of energy and where organic compounds have been detected or can reasonably be inferred (Mars, Europa). Category III applies to worlds where conditions are physically extreme but possibly capable of supporting exotic forms of life unknown on Earth (Titan, Triton). Category IV applies to bodies that could have seen the origin of life prior to the development of conditions so harsh as to make its perseverance at present unlikely but conceivable in isolated habitats (Venus, Io). Category V would be reserved for sites where conditions are so unfavorable for life by any reasonable definition that its origin or persistence there cannot be rated a realistic probability (the Sun, gas giant planets). The proposed system is intended to be generic. It assumes that life is based on polymeric chemistry occurring in a liquid medium with uptake and degradation of energy from the environment. Without any additional specific assumptions about the nature of life, the POL system is universally applicable.     

火星探测的主要科学问题

在太阳系的行星中,火星与地球之间存在最多的相似之处,因此,火星是一颗承载人类最多梦想的星球。火星有水和生命存在的问题,激发了人类火星探索的好奇心,成为人类持续探测火星的推动力。火星的起源和演化与太阳系形成过程的关系,火星与类地行星的共性和特性,是当代行星科学研究的重要内容。为了人类社会的可持续发展,火星可否改造成为适宜人类居住的绿色星球——这些是人类在火星探测中必须面对的重大科学问题。只有这些重大科学问题被一一解答,我们才能清晰地去思考地球和人类自身的未来！     

Survival and germinability of Bacillus subtilis spores exposed to simulated Mars solar radiation: implications for life detection and planetary protection

Bacterial spores have been considered as microbial life that could survive interplanetary transport by natural impact processes or human spaceflight activity. Deposition of terrestrial microbes or their biosignature molecules onto the surface of Mars could negatively impact life detection experiments and planetary protection measures. Simulated Mars solar radiation, particularly the ultraviolet component, has been shown to reduce spore viability, but its effect on spore germination and resulting production of biosignature molecules has not been explored. We examined the survival and germinability of Bacillus subtilis spores exposed to simulated martian conditions that include solar radiation. Spores of B. subtilis that contain luciferase resulting from expression of an sspB-luxAB gene fusion were deposited on aluminum coupons to simulate deposition on spacecraft surfaces and exposed to simulated Mars atmosphere and solar radiation. The equivalent of 42 min of simulated Mars solar radiation exposure reduced spore viability by nearly 3 logs, while germination-induced bioluminescence, a measure of germination metabolism, was reduced by less than 1 log. The data indicate that spores can retain the potential to initiate germination-associated metabolic processes and produce biological signature molecules after being rendered nonviable by exposure to Mars solar radiation.     

Hydrogen cyanide polymerization: a preferred cosmochemical pathway

Current research in cosmochemistry shows that crude organic solids of high molecular weight are readily formed in planetary, interplanetary and interstellar environments. Underlying much of this ubiquitous chemistry is a low energy route leading directly to the synthesis of hydrogen cyanide and its polymers. Evidence from laboratory and extraterrestrial investigations suggests that these polymers plus water yield heteropolypeptides, a truly universal process that accounts not only for the past synthesis of protein ancestors on Earth but also for reactions proceeding elsewhere today within our solar system, on planetary bodies and satellites around other stars and in the dusty molecular clouds of spiral galaxies. The existence of this preferred pathway - hydrogen cyanide polymerization - surely increases greatly the probability that carbon-based life is widespread in the universe.     

Special regions in Mars exploration: Problems and potential

“Special regions” on Mars are areas designated in the COSPAR planetary protection policy as areas that may support Earth microbes inadvertently introduced to Mars, or that may have a high probability of supporting indigenous martian life. Since absolutely nothing is known about martian life, the operational definition of a special region is a place that may allow the formation and maintenance of liquid water, on or under the surface of Mars. This paper will review the special-regions concept, the implications of recent recommendations on avoiding them, and the work of the Mars science community in providing an operational definition of those areas on Mars that are “non-special.”     

Future robotic exploration using honeybee search strategy: Example search for caves on Mars

Autonomous control has an increasing role in Earth and Space based applications. High level autonomy can greatly improve planetary exploration and is, in many cases, essential. It has been suggested during the Mars cave exploration programme, that an effective way to explore a larger surface area would be the use of many, small and fully autonomous robots. However, there are many challenges to overcome if such a swarm exploration programme is to be implemented. This paper summarises these challenges and focuses on one of the most crucial one: strategy. Many effective group exploration behaviours can be observed in nature, most of which are optimised to work with agents that have limited capabilities as individuals. For this paper a computer program has been written to simulate the way bees search for new hives and investigate whenever it is an optimal method to search for cave entrances on Mars. It has been found that this method, using simple autonomous robots which can be constructed using available technologies, could greatly improve the speed and range of a planetary exploration mission. The simulation results show that 50 swarm robots can cover an area of over 300 meters square completely in 5 sols while they are searching for cave entrances and returning results to the Lander which is a major performance improvement on any previous mission. Furthermore areas of interests found by the explorers are sorted in order of importance automatically and without the need of computational analysis, hence larger quantities of data were collected from the more important areas. Therefore the system – just like a hive of bees – can make a complex decision easily and quickly to find the place which matches the required criteria best. Using a high performance search strategy such as the one described in this paper is crucial if we plan to search for important resources or even life on Mars and other bodies in the solar system.     

Critical issues in connection with human planetary missions: protection of and from the environment.

Activities associated with human missions to the Moon or to Mars will interact with the environment in two reciprocal ways: (i) the mission needs to be protected from the natural environmental elements that can be harmful to human health, the equipment or to their operations: (ii) the specific natural environment of the Moon or Mars should be protected so that it retains its value for scientific and other purposes. The following environmental elements need to be considered in order to protect humans and the equipment on the planetary surface: (i) cosmic ionizing radiation, (ii) solar particle events; (iii) solar ultraviolet radiation; (iv) reduced gravity; (v) thin atmosphere; (vi) extremes in temperatures and their fluctuations; (vii) surface dust; (viii) impacts by meteorites and micrometeorites. In order to protect the planetary environment. the requirements for planetary protection as adopted by COSPAR for lander missions need to be revised in view of human presence on the planet. Landers carrying equipment for exobiological investigations require special consideration to reduce contamination by terrestrial microorganisms and organic matter to the Greatest feasible extent. Records of human activities on the planet's surface should be maintained in sufficient detail that future scientific experimenters can determine whether environmental modifications have resulted from explorations. Grant numbers: 14056/99/NL/PA.     

Can identification of a fourth domain of life be made from sequence data alone, and could it be done on Mars?

A central question in astrobiology is whether life exists elsewhere in the universe. If so, is it related to Earth life? Technologies exist that enable identification of DNA- or RNA-based microbial life directly from environmental samples here on Earth. Such technologies could, in principle, be applied to the search for life elsewhere; indeed, efforts are underway to initiate such a search. However, surveying for nucleic acid-based life on other planets, if attempted, must be carried out with caution, owing to the risk of contamination by Earth-based life. Here we argue that the null hypothesis must be that any DNA discovered and sequenced from samples taken elsewhere in the universe are Earth-based contaminants. Experience from studies of low-biomass ancient DNA demonstrates that some results, by their very nature, will not enable complete rejection of the null hypothesis. In terms of eliminating contamination as an explanation of the data, there may be value in identification of sequences that lie outside the known diversity of the three domains of life. We therefore have examined whether a fourth domain could be readily identified from environmental DNA sequence data alone. We concluded that, even on Earth, this would be far from trivial, and we illustrate this point by way of examples drawn from the literature. Overall, our conclusions do not bode well for planned PCR-based surveys for life on Mars, and we argue that other independent biosignatures will be essential in corroborating any claims for the presence of life based on nucleic acid sequences.     

Exchange of meteorites (and life?) between stellar systems

It is now generally accepted that meteorite-size fragments of rock can be ejected from planetary bodies. Numerical studies of the orbital evolution of such planetary ejecta are consistent with the observed cosmic ray exposure times and infall rates of these meteorites. All of these numerical studies agree that a substantial fraction (up to one-third) of the ejecta from any planet in our Solar System is eventually thrown out of the Solar System during encounters with the giant planets Jupiter and Saturn. In this paper I examine the probability that such interstellar meteorites might be captured into a distant solar system and fall onto a terrestrial planet in that system within a given interval of time. The overall conclusion is that it is very unlikely that even a single meteorite originating on a terrestrial planet in our solar system has fallen onto a terrestrial planet in another stellar system, over the entire period of our Solar System's existence. Although viable microorganisms may be readily exchanged between planets in our solar system through the interplanetary transfer of meteoritic material, it seems that the origin of life on Earth must be sought within the confines of the Solar System, not abroad in the galaxy.     

Rosetta: The ESA comet rendezvous mission

Rosetta was selected in November 1993 for the ESA Cornerstone 3 mission, to be launched in 2003, dedicated to the exploration of the small bodies of the solar system (asteroids and comets). Following this selection, the Rosetta mission and its spacecraft have been completely reviewed: this paper presents the studies performed the proposed mission and the resulting spacecraft design.

Three mission opportunities have been identified in 2003–2004, allowing rendezvous with a comet. From a single Ariane 5 launch, the transfer to the comet orbit will be supported by planetary gravity assists (two from Earth, one from Venus or Mars); during the transfer sequence, two asteroid fly-bys will occur, allowing first mission science phases. The comet rendezvous will occur 8–9 years after launch; Rosetta will orbit around the comet and the main science mission phase will take place up to the comet perihelion (1–2 years duration).

The spacecraft design is driven (i) by the communication scenario with the Earth and its equipment, (ii) by the autonomy requirements for the long cruise phases which are not supported by the ground stations, (iii) by the solar cells solar array for the electrical power supply and (iv) by the navigation scenario and sensors for cruise, target approach and rendezvous phases. These requirements will be developed and the satellite design will be presented.     

Mars through the looking glass: an interdisciplinary analysis of forward and backward contamination

The exploration of the planet Mars represents a significant milestone in humanity's exploration of the Universe. In the quest to better explore and understand Mars, issues of forward and backward contamination are particularly pertinent. This paper provides an interdisciplinary analysis of forward and backward contamination. Its substantive material reflects the scientific and technical concerns through the looking glass of legal and policy issues, and vice versa. The paper critically surveys contamination issues of current, nascent and proposed Mars missions from a scientific viewpoint. It then makes a comparative review of legal and policy mechanisms designed to protect against such contamination. It draws cross-linkages between the sciences and the law in this area. The paper then delineates a suggested interdisciplinary framework to protect against forward and backward contamination. This framework is based on co-operation between the various nations undertaking Mars missions, as well as between the various fields of expertise. It highlights the importance of an interdisciplinary overview in the implementation of measures protecting against forward and backward contamination. Strategies on the implementation of these measures are also outlined.     

Planetary parks—formulating a wilderness policy for planetary bodies

Building on an earlier article, establishment of a planetary park system for other planetary bodies is further developed. Reasons are elaborated for such a system to protect representative regions of other planetary bodies. Although a parks system might seem supererogatory, and an over-reaction to the currently very limited environmental impact of robotic and human exploration and settlement activities, four arguments are provided that suggest that such a system does have a value, even in advance of robotic and human missions. Planetary parks incorporate concepts of planetary protection, but they extend the reasons for practical protection policies beyond the utilitarian protection of scientific resources emphasized by planetary protection, into other utilitarian and intrinsic value arguments. Planetary parks might still allow for the development of non-park areas by commercial enterprise.     

Glycine identification in natural jarosites using laser desorption Fourier transform mass spectrometry: implications for the search for life on Mars

The jarosite group minerals have received increasing attention since the discovery of jarosite on the martian surface by the Mars Exploration Rover Opportunity. Given that jarosite can incorporate foreign ions within its structure, we have investigated the use of jarosite as an indicator of aqueous and biological processes on Earth and Mars. The use of laser desorption Fourier transform mass spectrometry has revealed the presence of organic matter in several jarosite samples from various locations worldwide. One of the ions from the natural jarosites has been attributed to glycine because it was systematically observed in combinations of glycine with synthetic ammonium and potassium jarosites, Na(2)SO(4) and K(2)SO(4). The ability to observe these organic signatures in jarosite samples with an in situ instrumental technique, such as the one employed in this study, furthers the goals of planetary geologists to determine whether signs of life (e.g., the presence of biomolecules or biomolecule precursors) can be detected in the rock record of terrestrial and extraterrestrial samples.     

Bacterial growth at the high concentrations of magnesium sulfate found in martian soils

The martian surface environment exhibits extremes of salinity, temperature, desiccation, and radiation that would make it difficult for terrestrial microbes to survive. Recent evidence suggests that martian soils contain high concentrations of MgSO? minerals. Through warming of the soils, meltwater derived from subterranean ice-rich regolith may exist for an extended period of time and thus allow the propagation of terrestrial microbes and create significant bioburden at the near surface of Mars. The current report demonstrates that halotolerant bacteria from the Great Salt Plains (GSP) of Oklahoma are capable of growing at high concentrations of MgSO? in the form of 2 M solutions of epsomite. The epsotolerance of isolates in the GSP bacterial collection was determined, with 35% growing at 2 M MgSO?. There was a complex physiological response to mixtures of MgSO? and NaCl coupled with other environmental stressors. Growth also was measured at 1 M concentrations of other magnesium and sulfate salts. The complex responses may be partially explained by the pattern of chaotropicity observed for high-salt solutions as measured by agar gelation temperature. Select isolates could grow at the high salt concentrations and low temperatures found on Mars. Survival during repetitive freeze-thaw or drying-rewetting cycles was used as other measures of potential success on the martian surface. Our results indicate that terrestrial microbes might survive under the high-salt, low-temperature, anaerobic conditions on Mars and present significant potential for forward contamination. Stringent planetary protection requirements are needed for future life-detection missions to Mars.     

Venus, Mars, and the ices on Mercury and the moon: astrobiological implications and proposed mission designs

Venus and Mars likely had liquid water bodies on their surface early in the Solar System history. The surfaces of Venus and Mars are presently not a suitable habitat for life, but reservoirs of liquid water remain in the atmosphere of Venus and the subsurface of Mars, and with it also the possibility of microbial life. Microbial organisms may have adapted to live in these ecological niches by the evolutionary force of directional selection. Missions to our neighboring planets should therefore be planned to explore these potentially life-containing refuges and return samples for analysis. Sample return missions should also include ice samples from Mercury and the Moon, which may contain information about the biogenic material that catalyzed the early evolution of life on Earth (or elsewhere). To obtain such information, science-driven exploration is necessary through varying degrees of mission operation autonomy. A hierarchical mission design is envisioned that includes spaceborne (orbital), atmosphere (airborne), surface (mobile such as rover and stationary such as lander or sensor), and subsurface (e.g., ground-penetrating radar, drilling, etc.) agents working in concert to allow for sufficient mission safety and redundancy, to perform extensive and challenging reconnaissance, and to lead to a thorough search for evidence of life and habitability.     

On biogenicity criteria for endolithic microborings on early Earth and beyond

Micron-sized cavities created by the actions of rock-etching microorganisms known as euendoliths are explored as a biosignature for life on early Earth and perhaps Mars. Rock-dwelling organisms can tolerate extreme environmental stresses and are excellent candidates for the colonization of early Earth and planetary surfaces. Here, we give a brief overview of the fossil record of euendoliths in both sedimentary and volcanic rocks. We then review the current understanding of the controls upon the distribution of euendolithic microborings and use these to propose three lines of approach for testing their biogenicity: first, a geological setting that demonstrates a syngenetic origin for the euendolithic microborings; second, microboring morphologies and distributions that are suggestive of biogenic behavior and distinct from ambient inclusion trails; and third, elemental and isotopic evidence suggestive of biological processing. We use these criteria and the fossil record of terrestrial euendoliths to outline potential environments and techniques to search for endolithic microborings on Mars.