The role of weightlessness in the genetic damage from preflight gamma-irradiation of organisms in experiments aboard the Salyut 6 orbital station

The effect of weightlessness on chromosomal aberration frequency in preflight irradiated $\text{Crepis capillaris}$ seeds, on the viability, fertility and mutation frequency in $\text{Arabidopsis thaliana}$, and on the frequency of nondisjunction and loss of X chromosomes in preflight irradiated $\text{Drosophila melanogaster}$ gametes was studied aboard the Salyut 6 orbital station. The following effects were observed: a flight-time dependent amplification of the effects of preflight

-irradiation in $\text{A}$. $\text{thaliana}$ with respect to all the parameters studied; unequal effects in seeds and seedlings of $\text{Crepis capillaris}$; and a significant increase in the frequency of nondisjunction and loss of chromosomes during meiosis in $\text{Drosophila}$ females. These observations are discussed in terms of the data of ground-based model experiments and flight experiments with a different time of exposure of objects to weightlessness. An attempt is made to elucidate the role of weightlessness in the modification of ionizing radiation effects.     

Optical and electron-microscopic studies of the Funaria hygrometrica protonema after cultivation for 96 days in space

$\text{Funaria hygrometrica}$ protonema cells grown in the “IFS-2” (Inoculating fixing system) for 96 days on board the Salyut 6 — Soyuz 32 orbital scientific station were examined by light and electron-microscopy. Investigation of experimental and control cells of the moss protonema showed common features as well as distinctions in their structure. Protonema cells of $\text{Funaria hygrometrica}$ both differentiate and undergo photosynthesis during space flight. Changes in cell shape, decreased cell size, a reduction in the volume of starch granules, and altered chloroplast structure were observed.     

Solar UV and ozone balloon measurements

Absolute solar UV spectra were obtained with a $\text{1}\text{4}$m spectrometer on a balloon flight from Palestine, Texas on September 23, 1981. This balloon reached a float altitude of 39 km at solar noon. The ozone density profiles derived from these spectra are discussed. The measurements are compared with data obtained from the same calibrated instrument flown in 1976 at solar minimum.     

The action of simulated and true weightlessness on the digestive tract of rats

Rats on board the Soviet Cosmos 936 satellite for 18$\text{1}\text{2}$ days showed a decreased glycoprotein secretion from the salivary mucous glands, stomach and intestine, and an increased leucine aminopeptidase and acid phosphatase content from the small intestine. Grimelius positive cells were activated. One group of rats were centrifuged at 1 g during the flight to simulate terrestrial gravity. Some investigations have suggested that under these conditions muscular and cardiac disorders diminished. In the digestive tract the benefits of centrifugation at 1 g are minimal and limited to a few glycoprotein components. The digestive changes are probably the expression of a stress response, unrelated to weightlessness. Similar changes, concomitant with a glycocorticoid hypersecretion, were found in rats after 15 days of hypokinesia on Earth. These digestive changes persisted even in adrenalectomized rats.     

The role of calcium ions in cytological effects of hypogravity

Electron-cytochemical and biochemical methods made it possible to reveal certain differences in ATPase activity stimulation by calcium ions in root apex cells of pea seedlings and moss protonema $\text{Funaria}$$\text{hygrometrica}$ grown under stationary and slow clinostatic (2 rev/min) conditions. It was showed that under clinostatic conditions in comparison with the control variant the ATPase activity decreases in plasmalemma. The protein content in the plasmalemma fraction was also twice as low under these conditions. The root apex cells of the pea seedlings grown under spaceflight conditions were found to contain high concentrations of membrane-bound calcium. The data obtained are discussed in relation to problems of possible mechanisms of disturbance in calcium balance and the system of active calcium ion transport through plasmalemma under hypogravity.     

On different sensitivities of microorganisms to lowered gravitation

The influence of lowered gravitation on biomass and CO₂ production in $\text{B.megaterium}$, a xerophyte, and $\text{Spirillum azotocolligens}$, an aqueous spirillum, in liquid nutrient medium on a horizontal clinostat at 0.1 $\text{g}$ has been studied. As controls we considered: 1) growth under stationary conditions of cultivation with test tubes oriented horizontally; 2) growth on a synchronously revolving centrifuge; and 3) growth on a swing with stirring. A horizontal clinostat at 0.1 g stimulates biomass production and CO₂ release in $\text{B.megaterium}$ as compared with the controls. $\text{Spirillum azotocolligens}$ growth is reduced as a result of clinostating. The best development and CO₂ production are observed under stationary conditions. The results do not support the assumption that microorganisms living in water are more resistant to lowered gravitation than those living in soil.     

Standard data procedures for future space projects

After a general definition of data- and instrument autonomy and an introduction to the End-to-End Data System concept the future guidelines for “Packet Telemetry and -Telecommand” are overviewed. These and other guidelines have been initiated by a NASA/ESA working group and further developed by an international “$\text{C}$onsultative $\text{C}$ommittee for $\text{S}$pace $\text{D}$ata $\text{S}$ystems” (CCSDS), coordinated with 6 space agencies. The status of these documents is reviewed, especially the document on Packet Telemetry, which has reached a mature stage. A pilot project, $\text{A}$utonomous $\text{P}$ayload $\text{C}$ontrol (APC), for the study and the demonstration of these new procedures is introduced shortly.     

Evidence for mass-loading of the Venus magnetosheath

The observed magnetic field configuration in the Venus magnetosheath contains information about the solar wind mass-loading processes occurring as a result of the extension of the neutral atmosphere into the magnetosheath. In this paper, magnetic field signatures of various mass-loading processes are discussed and experimental results from the Pioneer Venus Orbiter magnetometer experiment are examined for evidence of these signatures. The data suggest that the ?$\text{V}$X$\text{B}$ acceleration process, stochastic pickup of ionospheric ions, and $\text{J}$X$\text{B}$ force “scavenging” at the ionopause all occur at various times.     

The influence of space flight factors on viability and mutability of plants.

The experiments with air-dried Crepis capillaris seeds aboard the Soyuz 16 spaceship and the orbital stations Salyut 5, 6, 7 have revealed an increase in the frequency of aberrant cells in seedlings grown from flight-exposed seeds during the flight (experiment) and after the flight on Earth (flight control) as compared to the ground-based control. The increase in seedlings grown during the flight is more significant than in the flight control. During the flight Arabidopsis thaliana developed from cotyledons to the flowering stage. Analysis of seeds setting on these plants after the flight has shown a reduction in the fertility of these plants and an increase in the frequency of recessive mutants ("Light block-1"). An increased frequency of mutants was also retained in the progeny of plants which had passed through a complete cycle of development during the flight ("Fiton-3"). Suppression of embryo viability was observed in all experiments and expressed itself in reduced germinating ability of seeds from the exposed plants and in the early death of seedlings. Damages resulting from chromosome aberrations are eliminated in the first postflight generation and damages resulting from gene mutations and micro-aberrations are preserved for a longer time.     

Magnetic flux ropes in the Venus ionosphere: In situ observations of force-free structures?

Force-free magnetic structures with cylindrical geometry appear under a variety of conditions in nature. Filamentary helical magnetic structures are observed to be associated with prominences and flares in the solar atmosphere, and can arise in superconductors and laboratory plasmas. Another example of cylindrical quasi-force-free configurations appears to exist in the Venus ionosphere. Magnetic flux ropes with diameters of ～20 – 30 km have been observed by the Pioneer Venus Orbiter to be a nearly ubiquitous feature of the dayside Venus ionosphere. Models of flux ropes suggest that many of these structures tend to be quasi-force-free, i.e., $\text{J}$×$\text{B}$～0, while others are correlated with pressure variations in the ambient thermal plasma, $\text{J}$×$\text{B}$=-?(nkT).     

Ultraviolet spectroscopy of cometary comae: An update

Since its launch in 1978 the $\text{International Ultraviolet Explorer}$ ($\text{IUE}$) satellite observatory has been used to record ultraviolet spectra of nearly two dozen comets. These observations have been applied principally to studies of the composition, chemistry and evolution of the gaseous coma and more recently, with the substantially increased data base, to comparative analyses. The observations of Comets Bowell (1982 I) and Cernis (1983?) at a heliocentric distance of ≈ 3.4 AU show these two comets to be virtually identical and pose problems for water ice vaporization models. The most significant recent result from $\text{IUE}$ was the discovery of S₂ in the Earth-approaching comet IRAS-Araki-Alcock (1983d) and the use of the S₂ emission as a monitor of short-term variations in cometary activity. In early 1984, periodic comet Encke was observed for the second time by $\text{IUE}$, this time post-perihelion.     

The state experiment — mesospheric dynamics

The $\text{ST}$ructure and $\text{A}$tmospheric $\text{T}$urbulence $\text{E}$nvironment (STATE) experiment was conducted during the second week of June 1983 at Poker Flat Research Range, Alaska. The measurements focus on a study of the middle atmosphere dynamics by comparison between in-situ probe measurements and MST radar measurements. Rocket launchings were conducted at three periods which were selected by monitoring the doppler velocity spectra of the MST radar.The STATE program has included the efforts of several scientists in planning and carrying out the ground-based and rocket measurements. An overview of the program is given together with some preliminary results. The regions in intense backscatter signals detected by the MST radar are shown to correlate with large irregularities in the electron profiles measured.     

Temperature measurements during the CAMP program

The Cold Arctic Mesopause Program (CAMP) was conducted at ESRANGE, Sweden, in July/August 1982. During the time period of several weeks, the temperature was monitored by ground-based OH emission spectrometers and by stellite radiance measurements. Rocket launchings occurred on the nights of $\text{3}\text{4}$ and $\text{11}\text{12}$ August. On $\text{3}\text{4}$ August, seven rocket payloads were launched during a period of noctilucent cloud sighting over ESRANGE. The presence of the NLC was confirmed by several rocket-borne photometer profiles. The temperature measurements showed that the temperature profiles in the stratosphere and lower mesosphere were near the expected values of high latitude summer models. A large amplitude wave structure with three temperature minima of 139K, 114K and 111K were observed at altitudes between 83 and 94 km. The temperature minimum at 83 km was the location of the observed NLC. The temperature minima caused by the growth of the gravity wave amplitude in the highly stable mesosphere provide the regions for the growth of particles by nucleation to optical scattering size, as well as regions where the nuclei for condensation can be formed through ion chemistry paths.     

Ralicon anodes fabricated by electron beam lithography for image photon counting detectors

The Anger wedge and strip anode event location system developed for microchannel plate image photon detectors at the Space Sciences Laboratory of the University of California, Berkeley, has been extended in the present work by the use of electron beam lithography (EBL). Computer-aided design methods have been used to develop several types of RALICON ($\text{R}$eadout $\text{A}$nodes of $\text{Lit}$hographic $\text{Con}$struction) for use in photon counting microchannel plate imaging detectors. These anodes are suitable for linear, two dimensional or radial position measurements and they incorporate novel design features made possible by the EBL fabrication technique which significantly extend their application relative to published wedge-strip anode designs.     

Preliminary results of the Giotto radio science experiment

Doppler and ranging measurements using the radio signal of the GIOTTO spacecraft were taken before, during, and after the encounter with Comet Halley on $\text{13}\text{14}$ March 1986. The spacecraft velocity was found to decrease by a total of 23.3 cm s^?1 due to impacting gas and (primarily) dust in the cometary atmosphere. A preliminary dust production rate $\text{Q}{}_{\mathrm{d}}\text{? 10 × 10}{}^3\text{kg s}{}^{\mathrm{?1}}$ is found to be consistent with this deceleration. Power spectra of the carrier phase fluctuations reveal an increase in level and a flattening of the spectrum just prior to encounter, presumably associated with the enhanced dust impact rate. Finally, simulated Doppler time profiles are computed using the radial dependence of plasma density observed by the GIOTTO $\text{in situ}$ investigations. It is shown that the cometary electron content profile would have been clearly seen if a dual-frequency downlink radio configuration had been available at encounter.     

Human adaptation to simulated gravitational fields

We present the results of manned studies in which test subjects were exposed to simulated zero $\text{g}$ (water immersion or head-down tilt at ?6°) and head-to-feet acceleration. The findings give evidence that humans have different individual tolerances to an acceleration of +3 G_z after exposure to zero $\text{g}$, whether simulated by immersion or by head-down tilt. The paper discusses the functional relationship between water balance and cardiac output in the establishment of adaptive reactions to simulated zero $\text{g}$.     

Biological investigations aboard the biosatellite Cosmos-1129

Experiments on insects, higher plants and lower fungi were carried out aboard the biological satellite Cosmos-1129, in Earth orbit, from 25 September to 14 October 1979. The main objective of these experiments was to gain more profound knowledge of the effect of weightlessness on living organisms and to study the mechanisms by which these various organisms with different life cycles can adjust and develop in weightlessness. Experiments on insects ($\text{Drosophila melanogaster}$) were made with a view towards understanding gravitational preference in flies, the life cycle of which took place on board the biosatellite under conditions of artificial gravity. Experiments on higher plants ($\text{Zea mays, Arabidopsis taliana, Lycopersicum esculentum}$) and lower fungi ($\text{Physarum polycephalum}$) were performed.