In vitro plant cell growth in microgravity and on clinostat.

For the study of gravity's role in the processes of plant cell differentiation in-vitro, a model "seed-seedling-callus" has been used. Experiments were carried out on board the orbital stations Salyut-7 and Mir as well as on clinostat. They lasted from 18 to 72 days. It was determined that the exclusion of a one-sided action of gravity vector by means of clinostat and spaceflight conditions does not impede the formation and growth of callus tissue; however, at cell and subcellular levels structural and functional changes do take place. No significant changes were observed either on clinostat or in space concerning the accumulation of fresh biomass, while the percentage of dry material in space is lower than in control. Both in microgravity (MG) and in control, even after 72 days of growth, cells with a normally developed ultrastructure are present. In space, however, callus tissue more often contains cells in which the cross-section area of a cell, a nuclei and of mitochondria are smaller and the vacuole area--bigger than in controls. In microgravity a considerable decrease in the number of starch-containing cells and a reduction in the mean area of starch grains in amyloplasts is observed. In space the amount of soluble proteins in callus tissue is 1.5 times greater than in control. However, no differences were observed in fractions when separated by the SDS-PAGE method. In microgravity the changes in cell wall material components was noted. In the space-formed callus changes in the concentration of ions K, Na, Mg, Ca and P were observed. However, the direction of these changes depends on the age of callus. Discussed are the possible reasons for modification of morphological and metabolic parameters of callus cells when grown under changed gravity conditions.     

Ultrastructural analysis of organization of roots obtained from cell cultures at clinostating and under microgravity.

Data are presented of a comparative analysis on rhizogenesis in the Arabidopsis thaliana tissue culture growing in a solid nutrient medium under stationary conditions, clinostatic conditions and microgravity. Tissue samples weighing 100 mg. were set in the Petri dishes and placed in a horizontal slow clinostat /2 revs/min/. After 14 days of growth they were analyzed. On clinostating the number of roots formed from the callus cells was approximately one half the control. The formed root cap manifested no essential differences, in comparison with the stationary control, in the number of layers and cell sizes in its layers. In callusogenic roots, formed from clinostated cells, differentiation including root cap cells, proceeds without noticeable deviations from the norm. At the same time, gravireceptor cells do not function under these conditions. This is clearly displayed at a structural level in the location of amyloplasts-statoliths throughout the cytoplasm. The callus cell cultures experienced microgravity for 8 days. The number of formed roots under the influence of this factor was 36% relative to the stationary control. Root cap formation was abnormal. Gravireceptor cells did not formed under microgravity.     

The effect of microgravity on the development of plant protoplasts flown on Biokosmos 9.

An experiment using plant protoplasts has been accepted for the IML-1 Space Shuttle mission scheduled for 1991. Preparatory experiments have been performed using both fast and slow rotating clinostats and in orbit to study the effect of simulated and real weightlessness on protoplast regeneration. Late access to the space vehicles before launch has required special attention since it is important to delay cell wall regeneration until the samples are in orbit. On a flight on Biokosmos 9 ("Kosmos-2044") in September 1989 some preliminary results were obtained. Compared to the ground control, the growth of both carrot and rapeseed protoplasts was decreased by 18% and 44% respectively, after 14 days in orbit. The results also indicated that there is less cell wall regeneration under micro-g conditions. Compared to the ground controls the production of cellulose in rapeseed and carrot flight samples was only 46% and 29% respectively. The production of hemicellulose in the flight samples was 63% and 67% respectively of that of the ground controls. In both cases all samples reached the stage of callus development. The peroxidase activity was also found to be lower in the flight samples than in the ground controls, and the number of different isoenzymes was decreased in the flight samples. In general, the regeneration processes were retarded in the flight samples with respect to the ground controls. From a simulation experiment for IML-1 performed in January 1990 at ESTEC, Holland, regenerated plants have been obtained. These results are discussed and compared to the results obtained on Biokosmos 9. Protoplast regeneration did not develop beyond the callus stage in either the flight or the ground control samples from the Biokosmos 9 experiment.     

Ontogeny of plants under various gravity condition.

The results of experiments performed under conditions of microgravity (MG) or under its simulation on the horizontal clinostat (HC) with the callus, seedlings of various species and embryogenic structures have revealed a definite role of gravity as an ecological factor in the processes of cytomorphogenesis, growth, and development. The transformation of differentiated somatic cells of arabidopsis seed into undifferentiated callus was not inhibited under MG, though modifications of the whole callus morphology and of mean cell and nucleus size were observed. The morphogenesis of polar structures such as root-hair bearing cells of Lactuca primary root has been shown to be modified in the course of differentiation under mass acceleration diminished below 0.1 g. Seed germination and seedling morphogenesis under MG follow their normal course, but a significant stimulation of shoot growth with no effect on primary root growth has been determined. A successful in vitro regeneration of Nicotiana tabacum plantlets from leaf cells and subsequent formation of shoots and roots on a continuously rotating HC as well as the formation of viable seeds during seed-to-seed growth of Arabidopsis plants under MG have indicated that gravity plays but a limited role in the processes of embryogenesis and organogenesis.     

Spaceflight opportunities on the ISS for plant research--the ESA perspective.

Two ESA facilities will be available for plant research and other biological experiments on the International Space Station: the Modular Cultivation System (MCS) and BIOLAB. While BIOLAB will be launched with the European "Columbus" Module, MCS will be part of the Early Utilisation Agreement with NASA and integrated in the US Lab. Both facilities use standard Experiment Containers, mounted on two centrifuge rotors providing either microgravity or variable g-levels up to 2xg. Transparent covers allow illumination and observation (also near-infrared) of the internal experiment hardware containing the plant specimen. Standard interface plates provide each container with power and data lines, gas supply (controlled CO2, O2 and water vapour concentration; ethylene removal), and--for MCS only--connectors to water reservoirs. Besides the two concepts of environmental control in both facilities, there is a difference in container size (BIOLAB 0.36 l, height with respect to the g-vector 60 mm; MCS 0.58 l, height 160 mm) and in the degree of automation. The design of BIOLAB and MCS will be complimentary to NASA's Plant Research Unit (volume 20 l, height 380 mm) and should allow continuation of Space research on protoplasts, callus cultures, algae, fungi and seedlings, as earlier flown on Biorack, and new experiments with larger specimens of fungi, mosses and vascular plants.     

虚拟匹配单元对力觉交互系统性能影响分析

讨论虚拟现实力觉交互系统中,阻抗再现方式下虚拟匹配单元对系统稳定性和透明性的影响.对不同的虚拟环境,根据弹簧单元和阻尼单元的不同物理性质,将其与虚拟环境串联或并联,作为连接操作端与虚拟端的双端口系统;对于单自由度力觉交互系统,当虚拟环境驱动点为弹簧元素时,以串联弹簧单元和串联阻尼单元为例,通过对附加虚拟匹配单元后的系统性能评价,及与原系统的比较,分析了虚拟匹配单元对系统性能尤其是稳定性的影响,仿真和分析结果吻合较好.在力觉交互控制系统设计中,可根据需要选择不同的虚拟匹配单元以提高系统的稳定性.      

Differenciation et proliferation cellulaires dans des racines de mais cultive en microgravite (Biocosmos 1985)

A cytological study was performed on Maize roots ( s) which were grown in space (flight Biocosmos 1985) and on control roots on earth. Two criteria were selected : cell elongation in the cortical zone in the four mm of the extremity of the root and mitotic activity of the meristem. The results show that in microgravity the length of the meristem is reduced of 1/3 and that its mitotic activity increases of about twofold comparatively to the synchronous control. In parallel the cell differentiation begin closer to the root cap junction. These results are discussed relative to the influence of gravistimulation on cell proliferation and cell differentiation in roots.     

用于铯光泵磁强计的无磁恒温控制系统设计

针对铯光泵磁强计对原子气室温度高稳定性及无磁干扰的要求，设计了一种用于铯光泵磁强计的无磁恒温控制系统。该系统主要包括无磁加热器件与无磁恒温控制电路。无磁加热器件采用微机电系统工艺的双层对称四线结构，可有效抑制电流产生的磁场；无磁恒温控制电路通过交流加热进一步减小恒定磁场干扰，以现场可编程门阵列为核心，使用直接数字式频率合成技术产生高频加热信号，再经功率放大进行无磁恒温控制，减少硬件电路资源。测试结果表明，气室恒温控制的温度噪声峰峰值可达到0.02 ℃，满足光泵磁强计的性能要求。     

Clinostation influence on regeneration of cell wall in Solanum tuberosum L. protoplasts.

Regeneration of cell walls in protoplasts was investigated using light- and electronmicroscopic methods. The protoplasts were isolated from mesophyll of Solanum tuberosum leaves and were cultivated on the horizontal low rotating clinostat (2 rpm) and in control for 10 days. Using a fluorescent method (with Calcofluor white) it was demonstrated that changes in vector gravity results in a regeneration inhibition of cell wall. With electron-microscopical and electro-cytochemical methods (staining with alcianum blue) dynamics of the regeneration of cell walls in protoplasts was studied; carbohydrate matrix of cell walls is deposited at the earliest stages of this process. The influence of microgravity on the cell wall regeneration is discussed in higher plants.     

The effects of extended clinorotation on potato minituber formation and structure.

Formation and structure of potato minitubers grown aseptically for 30 days on a horizontal clinostat and in stationary control have been studied by light and electron microscopy. It was demonstrated that the number of plants that formed minitubers, their size and fresh weight, was higher when clino-rotated than in the stationary control. It was revealed that the amount of amyloplasts in parenchyma cell sections was doubled in minitubers formed under clino-rotation. Other factors (shape of minitubers and size of reserve parenchyma cells) did not differ from the stationary control. The changes in amyloplast ultrastructure suggest accelerated cell maturity of potato reserve parenchyma in extended clino-rotation.     

The role of cellulases in the mechanism of changes of cell walls of Funaria hygrometrica moss protonema at clinostating.

Using electroncytochemical and biochemical methods, differences between the cytochemical reaction intensity and activity of the cellulosolytic enzymes in Funaria hygrometrica moss cells grown for 30 days in the horizontal clinostat (2 rev/min) and in control have been studied. It has been shown that on clinostating the precipitate amount and size increases with the cellulase activity enhancement in the periplasmic space and protonema cell walls, when compared to control. Using biochemical methods it has been found that the activity of both endo-1,4-beta-glucanase and exo-1,4-beta-glucanase was higher under these conditions. A decrease of cellulose total content, its crystalline form, and pectic substances as well as an increase of hemicellulose content have been revealed in the clinostated material compared to control. Data obtained are discussed regarding the possible mechanism of cellulase activation and synthesis inhibition and cellulose crystallization in plant cell walls at clinostating.     

Long clinostation influence on the localization of free and weakly bound calcium in cell walls of Funaria hygrometrica moss protonema cells.

The pyroantimonate method was used to study the localization of free and weakly bound calcium in cells of moss protonema of Funaria hygrometrica Hedw. cultivated on a clinostat (2rev/min). Electroncytochemical study of control cells cultivated at 1 g revealed that granular precipitate marked chloroplasts, mitochondria, Golgi apparatus, lipid drops, nucleoplasma, nucleolus, nucleus membranes, cell walls and endoplasmic reticulum. In mitochondria the precipitate was revealed in stroma, in chloroplast it was found on thylakoids and envelope membranes. The cultivation of protonema on clinostat led to the intensification in cytochemical reaction product deposit. A considerable intensification of the reaction was noted in endomembranes, vacuoles, periplasmic space and cell walls. At the same time analysis of pectinase localization was made using the electroncytochemical method. A high reaction intensity in walls in comparison to that in control was found out to be a distinctive peculiarity of the cells cultivated on clinostat. It testifies to the fact that increasing of free calcium concentrations under conditions of clinostation is connected with pectinic substances hydrolysis and breaking of methoxy groups of pectins. Data obtained are discussed in relation to problems of possible mechanisms of disturbance in calcium balance of plant cells and the role of cell walls in gomeostasis of cell grown under conditions of simulated weightlessness.     

原子磁强计原子气室无磁加热温控系统设计

介绍一种用于原子磁强计原子气室的无磁加热温控系统设计。针对温控系统加热电流产生的磁场干扰,设计了双层加热丝加热膜结构的加热器件,该加热器件利用同层平行反向电流和层间平行反向电流产生的磁场相互抵消以减小加热电流磁场噪声;设计了滑动平均滤波器对采集的温度信号实施滤波以减小随机噪声的干扰;结合设计的高频加热信号的产生电路、幅度控制电路和功率放大电路开展了实验测试。测试结果表明,研制的双层加热丝加热膜结构加热器件相比单层加热丝加热膜结构加热器件的电流磁场噪声抑制能力提高了约16倍,实现的无磁加热温控系统的温控能力达到1. 2‰。     

原子磁强计原子气室无磁加热温控系统设计

介绍一种用于原子磁强计原子气室的无磁加热温控系统设计。针对温控系统加热电流产生的磁场干扰,设计了双层加热丝加热膜结构的加热器件,该加热器件利用同层平行反向电流和层间平行反向电流产生的磁场相互抵消以减小加热电流磁场噪声;设计了滑动平均滤波器对采集的温度信号实施滤波以减小随机噪声的干扰;结合设计的高频加热信号的产生电路、幅度控制电路和功率放大电路开展了实验测试。测试结果表明,研制的双层加热丝加热膜结构加热器件相比单层加热丝加热膜结构加热器件的电流磁场噪声抑制能力提高了约16倍,实现的无磁加热温控系统的温控能力达到1.2‰。     

基于波导缝隙天线的TEM室频率扩展方法

为突破实际测试当中的局限,研究了横电磁波传输（TEM）室频率范围的扩展方法。运用电磁场与微波技术相关原理,通过分析TEM室壳体开缝对高次模的影响,利用壳体表面电流分布变化规律和波导缝隙天线原理重新解释了开缝对高次模的抑制作用,进而提出了一种TEM室壳体表面开缝的设计方法。通过电磁场数值仿真,验证了抑制高次模工程方法的有效性,并对其控制参数和约束条件的准确性进行了分析评估;通过加工实物并进行实测,进一步验证了新方法的效果。仿真与测试结果表明,开缝抑制高次模的工程方法能够在不减小测试空间与影响主模传输的前提下,将测试带宽扩展了42.9%。      

Combined effects of radiation and trauma.

Injuries, caused by both whole-body irradiation and wounds or burns, have been relatively little studied. Possibly because many investigators think that these injuries are just modified radiation-induced diseases for which the same treatment principles are valid. Other authors had the impression that, for instance, the radiation burn trauma is a new kind of disease which differs significantly from either radiation syndrome alone or from burn disease. There are many experimental data on animals which suggest that the pathology of combined injuries differs significantly from that of radiation-induced disease or of thermal or mechanical traumas. Wounds or burns which, in general, do not cause septicaemia could become entrance ports for bacteria when animals are exposed to whole-body irradiation. Thrombocytopenia is the reason for hemorrhages in wounds. The susceptibility to shock is increased considerably in combined injuries and the formation of callus in the bone fractures is significantly delayed. The healing of wounds and burns in the initial phase of the radiation syndrome does not always differ from healing in the non-irradiated organism. However, a few days or weeks later very serious wound infections and hemorrhages can occur. The additional injuries almost always worsen the development and prognosis of radiation-induced disease. The recommended treatment for combined injuries will differ in many respects from the treatment of wounds and burns or the radiation syndrome.     

燃料电池无人机动力系统半实物仿真

针对燃料电池无人机（UAV）动力系统飞行测试困难的问题,为了提升动力系统设计与开发水平,以燃料电池、锂电池、DC/DC功率转换器、电子调速器和直流无刷电机组成的动力系统作为实物介入,无人机动力学、自动驾驶仪、螺旋桨、飞行环境等数学模型为软件部分,无人机的油门信号控制及飞行过程中电机的载荷等为模拟仿真部分,以信号发生器、测功机及扭矩加载装置为软硬件接口,设计并搭建了燃料电池无人机动力系统半实物（HIL）仿真平台。面向典型任务剖面,基于状态机管理策略,对燃料电池/锂电池电动无人机的动力系统进行了半实物仿真研究,分析了半实物仿真平台和管理策略的有效性和实用性。      

Longevity in space; experiment on the life span of Paramecium cell clone in space.

Life span is the most interesting and also the most important biologically relevant time to be investigated on the space station. As a model experiment, we proposed an investigation to assess the life span of clone generation of the ciliate Paramecium. In space, clone generation will be artificially started by conjugation or autogamy, and the life span of the cell populations in different gravitational fields (microgravity and onboard 1 x g control) will be precisely assessed in terms of fission age as compared with the clock time. In order to perform the space experiment including long-lasting culture and continuous measurement of cell division, we tested the methods of cell culture and of cell-density measurement, which will be available in closed environments under microgravity. The basic design of experimental hardware and a preliminary result of the cultivation procedure are described.     

Longevity in space; Experiment on the life span of Paramecium cell clone in space

Life span is the most interesting and also the most important biologically relevant time to be investigated on the space station. As a model experiment, we proposed an investigation to assess the life span of clone generation of the ciliate Paramecium. In space, clone generation will be artificially started by conjugation or autogamy, and the life span of the cell populations in different gravitational fields (microgravity and onboard 1 x g control) will be precisely assessed in terms of fission age as compared with the clock time. In order to perform the space experiment including long-lasting culture and continuous measurement of cell division, we tested the methods of cell culture and of cell-density measurement, which will be available in closed environments under microgravity. The basic design of experimental hardware and a preliminary result of the cultivation procedure are described.