Repair and misrepair of heavy-ion-induced chromosomal damage.

The premature chromosome condensation (PCC) technique was used to investigate chromosomal damage, repair, and misrepair in the G phase of a human/hamster hybrid cell line that contains a single human chromosome. Plateau-phase cell cultures were exposed to either x-rays or a 425 MeV/u beam of neon ions near the Bragg peak where the LET is 183 kev/micrometers. An in situ hybridization technique coupled to fluorescent staining of PCC spreads confirmed the linearity of the dose response for initial chromatin breakage in the human chromosome to high doses (1600 cGy x-ray or 1062 cGy Ne). On Giemsa-stained slides, initial chromatin breakage in the total genome and the rejoining kinetics of these breaks were determined. As a measure of chromosomal misrepair, ring PCC aberrations were also scored. Ne ions were about 1.5 x more effective per unit dose compared to x-rays at producing the initially measured chromatin breakage. 90% of the x-ray-induced breaks rejoined in cells incubated at 37 degrees C after exposure. In contrast, only 50% of Ne-ion-induced breaks rejoined. In the irradiated G1 cells, ring PCC aberrations increased with time apparently by first order kinetics after either x-ray or Ne exposures. However, far fewer rings formed in Ne-irradiated cells after a dose giving a comparable initial number of chromatin breaks. Following x-ray exposures, the yield of rings formed after long repair times (6 to 9 hrs) fit a quadratic dose-response curve. These results indicate quantitative and qualitative differences in the chromosomal lesions induced by low- and high-LET radiations.     

Correlation between cell death and induction of non-rejoining PCC breaks by carbon-ion beams.

We have shown a correlation between cell death and induction of non-rejoining chromatin breaks in two normal human cells and three human tumor cell lines irradiated by carbon-ion beams and X rays. Non-rejoining chromatin breaks were measured by counting the number of remaining chromatin fragments detected by the premature chromosome condensation (PCC) technique. Carbon-ion beams were accelerated by the Heavy Ion Medical Accelerator in Chiba (HIMAC). The cells were irradiated by two different mono-LET beams (LET = 13 keV/micrometer and 77 keV/micrometer ) and 200 kV X rays. The RBE values of cell death for carbon-ion beams relative to X rays were 1.1 to 1.4 for 13 keV/micrometer beams and 2.5 to 2.9 for 77 keV/micrometer beams. The induction rate of non-rejoining PCC breaks per cell per Gy was found to be highest for the 77 keV/micrometer beams for all of the cell lines.The results found in this study show that there is a good correlation between cell death and induction of non-rejoining PCC breaks for these human cell lines.     

Residual chromatin breaks as biodosimetry for cell killing by carbon ions

We have studied the relationship between cell killing and the induction of residual chromatin breaks on various human cell lines and primary cultured cells obtained by biopsy from patients irradiated with either X-rays or heavy-ion beams to identify potential bio-marker of radiosensitivity for radiation-induced cell killing. The carbon-ion beams were accelerated with the Heavy Ion Medical Accelerator in Chiba (HIMAC). Six primary cultures obtained by biopsy from 6 patients with carcinoma of the cervix were irradiated with two different mono-LET beams (LET= 13 keV/μm, 76 keV/μm) and 200kV X rays. Residual chromatin breaks were measured by counting the number of non-rejoining chromatin fragments detected by the premature chromosome condensation (PCC) technique after a 24 hour postirradiation incubation period. The induction rate of residual chromatin breaks per cell per Gy was the highest for 76 keV/μm beams on all of the cells. Our results indicated that cell which was more sensitive to the cell killing was similarly more susceptible to induction of residual chromatin breaks. Furthermore there is a good correlation between these two end points in various cell lines and primary cultured cells. This suggests that the detection of residual chromatin breaks by the PCC technique may be useful as a predictive assay of tumor response to cancer radiotherapy.     

基于PCC-LSTM模型的短期负荷预测方法

短期负荷预测是电网合理调度和平稳运行的基础。为提高短期负荷预测精度,提出了一种基于Pearson相关系数(PCC)和长短期记忆(LSTM)神经网络的短期负荷预测方法。该方法运用Pearson相关性分析对原始多维输入变量组成的时间序列进行相关性分析,选取与电力负荷数据相关性较大的影响因素作为输入量,实现原始数据的降维和选优;再通过LSTM神经网络结合Adam优化算法,对与电力负荷相关性较大的影响因素和负荷实际输出序列之间的非线性关系建立网络模型。以嘉捷BOX和重庆丽苑维景国际大酒店的负荷数据作为实际算例,并与Prophet、LSTNet、门控循环(GRU)神经网络模型方法进行对比。结果表明:所提PCC-LSTM模型预测精度均在91%以上,最高可达95.44%,有效提高了负荷预测的精度。      

LET dependence of cell death, mutation induction and chromatin damage in human cells irradiated with accelerated carbon ions.

We investigated the LET dependence of cell death, mutation induction and chromatin break induction in human embryo (HE) cells irradiated by accelerated carbon-ion beams. The results showed that cell death, mutation induction and induction of non-rejoining chromatin breaks detected by the premature chromosome condensation (PCC) technique had the same LET dependence. Carbon ions of 110 to 124keV/micrometer were the most effective at all endpoints. However, the number of initially induced chromatin breaks was independent of LET. About 10 to 15 chromatin breaks per Gy per cell were induced in the LET range of 22 to 230 keV/micrometer. The deletion pattern of exons in the HPRT locus, analyzed by the polymerase chain reaction (PCR), was LET-specific. Almost all of the mutants induced by 124 keV/micrometer beams showed deletion of the entire gene, while all mutants induced by 230keV/micrometer carbon-ion beams showed no deletion. These results suggest that the difference in the density distribution of carbon-ion track and secondary electron with various LET is responsible for the LET dependency of biological effects.     

Chromosome aberrations in human lymphocytes induced by 250 MeV protons: effects of dose, dose rate and shielding.

Although the space radiation environment consists predominantly of energetic protons, astronauts inside a spacecraft are chronically exposed to both primary particles as well as secondary particles that are generated when the primary particles penetrate the spacecraft shielding. Secondary neutrons and secondary charged particles can have an LET value that is greater than the primary protons and, therefore, produce a higher relative biological effectiveness (RBE). Using the accelerator facility at Loma Linda University, we exposed human lymphocytes in vitro to 250 MeV protons with doses ranging from 0 to 60 cGy at three different dose rates: a low dose rate of 7.5 cGy/h, an intermediate dose rate of 30 cGy/h and a high dose rate of 70 cGy/min. The effect of 15 g/cm2 aluminum shielding on the induction of chromosome aberrations was investigated for each dose rate. After exposure, lymphocytes were incubated in growth medium containing phytohemagglutinin (PHA) and chromosome spreads were collected using a chemical-induced premature chromosome condensation (PCC) technique. Aberrations were analyzed using the fluorescence in situ hybridization (FISH) technique with three different colored chromosome-painting probes. The frequency of reciprocal and complex-type chromosome exchanges were compared in shielded and unshielded samples.     

Chromosome aberrations as biomarkers of radiation exposure: modelling basic mechanisms.

The space radiation environment is a mixed field consisting of different particles having different energies, including high charge and energy (HZE) ions. Conventional measurements of absorbed doses may not be sufficient to completely characterise the radiation field and perform reliable estimates of health risks. Biological dosimetry, based on the observation of specific radiation-induced endpoints (typically chromosome aberrations), can be a helpful approach in case of monitored exposure to space radiation or other mixed fields, as well as in case of accidental exposure. Furthermore, various ratios of aberrations (e.g. dicentric chromosomes to centric rings and complex exchanges to simple exchanges) have been suggested as possible fingerprints of radiation quality, although all of them have been subjected to some criticisms. In this context a mechanistic model and a Monte Carlo code for the simulation of chromosome aberration induction were developed. The model, able to provide dose-responses for different aberrations (e.g. dicentrics, rings, fragments, translocations, insertions and other complex exchanges), was further developed to assess the dependence of various ratios of aberrations on radiation quality. The predictions of the model were compared with available data, whose experimental conditions were faithfully reproduced. Particular attention was devoted to the scoring criteria adopted in different laboratories and to possible biases introduced by interphase death and mitotic delay. This latter aspect was investigated by taking into account both metaphase data and data obtained with Premature Chromosome Condensation (PCC).     

基于STM32的直流电机调速新方法

为满足不同的需求,经常使用PWM波调整直流电机的转速。但在实际应用中,即使是一个固定的PWM值,直流电机的转速也未必恒定。为了解决这个问题,通常用PID算法控制电机的转速,使其达到一个稳定值。但PID算法比较复杂,要进行建模、参数整定等。提出一种简单、可行的方法,在很短的定时时间内,测试电机的转速,将其与设定转速值进行比较,得到误差。根据误差值的正负,调整PWM波中的正脉宽的宽度,以达到对电机转速的快速、实时调整。经过实测：采用STM32微处理器,能够快速调整电机转速,并使其很快达到一个稳定值。     

Measuring the economic benefits of a national planetary protection policy to regulate future private space activities between Earth and Mars: Results of a contingent valuation survey in Greece

The emergence of private space actors may soon enable the growth of the novel market segments of space research and exploration, space resources utilization, and human access to space. The interdisciplinary field of Planetary Protection has to keep up with these advances. Planetary Protection is defined as a set of guidelines that aim to prevent the forward contamination of celestial bodies with biological material from Earth and the backward contamination of the terrestrial biosphere with extraterrestrial biological material. As space entrepreneurs acquire and develop the resources and competencies for commercial access to space, significant questions are expected to be raised in the future with respect to potential forward and backward contamination issues, particularly with respect to activities between Earth and Mars. Although such private activities do not seem to pose a serious Planetary Protection threat at the moment, certain preparatory steps need to be taken in order to prudently inform the relevant policy-making procedures. This work describes the application of the Contingent Valuation Method, a useful tool of the environmental economics discipline, with the aim of demonstrating a novel approach to estimate the economic valuation of the external benefits of preventing forward and backward contamination between Earth and Mars. Particularly, via a survey specifically developed for this purpose, a set of questions are used to elicit the perceived economic value that respondents place on the prevention of forward and backward contamination; the survey is administered to a national probability sample in Greece, and the generated data is processed through statistical analysis. The Contingent Valuation Method is a popular and well-established stated preference valuation technique; these techniques are often the more suitable choice for ex-ante valuations of future changes, and are currently the only known approach to capture all the aspects of the economic value of non-market goods. Through an initial proof-of-concept in Greece, the goal of this work is to provide useful insights on the expected external benefits of a national Planetary Protection policy to regulate future private space activities between Earth and Mars, and to encourage a larger-scale application of this tool in other countries around the world.     

企业的竞争战略与生产系统的动态发展

当今日益变化的市场对传统的生产系统提出了挑战.为了面对变化的市场,形成自我竞争优势,增强企业的应变能力,本文认为生产系统应具有持续的动态发展性,需要从战略的角度重新审视生产管理.目前,柔性化制造原则在生产管理者们心中的地位越来越高,无论是传统的工业企业还是全新的工业企业,对柔性化系统运用的需求在增长.本文介绍了柔性化原则在为企业减少或重新定义不确定性时的应用.     

A multi-objective optimization framework of constellation design for emergency observation

To meet the urgent needs of emergency observation missions, a well-designed constellation is key to ensuring system performance. This paper presents a multi-objective optimization framework which is well suited for constellation designers to identify key trade-offs and make decisions. Firstly, this work investigates the metrics of responsiveness and coverage simultaneously to approximate to the actual conditions of emergencies, and a multi-objective mathematical model is constructed to explore solutions to enhance the overall system performance. Then, according to the characteristics of emergency missions, a hierarchical chromosome encoding method is proposed in the framework to encode a solution that explores different sized constellations without constraining its specific geometry. Finally, we propose the use of radial axes plots to help constellation designers to gain pragmatic insights for informed decision making. The simulation of disaster management verifies that the proposed chromosome encoding method effectively promotes the convergence of the algorithm, and the multi-objective optimization framework helps to generate a rapid and adequate observation satellite constellation to provide maximum coverage performance while ensuring timeliness.     

Coronal mass ejection detection using wavelets,curvelets and ridgelets: Applications for space weather monitoring

Coronal mass ejections (CMEs) are large-scale eruptions of plasma and magnetic field that can produce adverse space weather at Earth and other locations in the Heliosphere. Due to the intrinsic multiscale nature of features in coronagraph images, wavelet and multiscale image processing techniques are well suited to enhancing the visibility of CMEs and suppressing noise. However, wavelets are better suited to identifying point-like features, such as noise or background stars, than to enhancing the visibility of the curved form of a typical CME front. Higher order multiscale techniques, such as ridgelets and curvelets, were therefore explored to characterise the morphology (width, curvature) and kinematics (position, velocity, acceleration) of CMEs. Curvelets in particular were found to be well suited to characterising CME properties in a self-consistent manner. Curvelets are thus likely to be of benefit to autonomous monitoring of CME properties for space weather applications.     

基于水下机器人的海产品智能检测与自主抓取系统

针对水下机器人实现自主抓取过程中缺乏引导系统的问题,提出了一种依托水下机器人的海产品智能检测与自主抓取系统,用来解决水下目标的智能检测问题,并引导水下机器人进行海产品的自主抓取。将卷积神经网络检测算法应用到水下场景,利用水下图像数据集训练特定的网络模型DSOD检测海产品。建立短基线定位系统定位水下作业的机器人。通过分析相机成像坐标系与定位系统坐标系之间的关系,提出了一种计算海产品实际位置的坐标转换方法,计算海产品的实际位置。设计了一种基于反馈机制的多信号分析方法,引导机器人在水下移动并抓捕海产品。为了验证所提系统的有效性,搭建了一款水下抓捕机器人,并成功将所提算法应用到机器人,在真实海洋环境中进行海产品的自主抓取实验。      

半球谐振子疲劳寿命分析与仿真

半球谐振子的寿命和损伤是直接影响高精度半球谐振陀螺使用时长和安全性的重要因素。目前国内加工的半球谐振子所用的熔融石英玻璃材料主要依靠进口,采用传统的疲劳寿命实验确定方法成本过于昂贵,因此需要利用疲劳分析软件对半球谐振子的疲劳寿命进行分析。文章通过半球谐振陀螺应力分析,采用ANSYS软件对熔融石英半球谐振子进行应力分析仿真,确定因残余应力所引起的疲劳危险部位,并在疲劳部位进行裂纹扩展分析,得到 、 和 型应力强度因子,以使在半球谐振子结构设计和使用过程中对易疲劳的部位进行有效监控和预防。     

Distributing space weather monitoring instruments and educational materials worldwide for IHY 2007: The AWESOME and SID project

The International Heliophysical Year (IHY) aims to advance our understanding of the fundamental processes that govern the Sun, Earth, and heliosphere. The IHY Education and Outreach Program is dedicated to inspiring the next generation of space and Earth scientists as well as spreading the knowledge, beauty, and relevance of our solar system to the people of the world. In our Space Weather Monitor project we deploy a global network of sensors to high schools and universities to provide quantitative diagnostics of solar-induced ionospheric disturbances, thunderstorm intensity, and magnetospheric activity. We bring real scientific instruments and data in a cost-effective way to students throughout the world. Instruments meet the objectives of being sensitive enough to produce research-quality data, yet inexpensive enough for placement in high schools and universities. The instruments and data have been shown to be appropriate to, and usable by, high school age and early university students. Data contributed to the Stanford data center is openly shared and partnerships between groups in different nations develop naturally. Students and teachers have direct access to scientific expertise.     

Phasing with near rectilinear Halo orbits: Design and comparison

In the next future, space agencies are planning to return to the Moon. The objective is to assemble an orbiting space station, called Gateway, on a Near Rectilinear Halo Orbit around the Moon as a base for future Moon and deep space missions. Within this framework, multiple side missions will be planned to sustain the Gateway (Artemis mission). The proposed work is thought in framework of the preliminary design of future cargo missions, in particular on the design of an efficient phasing trajectory, under the Circular Restricted Three body problem hypotheses, to bring a cargo vehicle from the end of the Earth-Moon transfer to the beginning of the proximity operations such as rendezvous and docking with the space station. The work aims covering the lack of literature in phasing trajectories with the NRHO by proposing three different strategies to connect the Earth-Moon transfer trajectory with the proximity operations. The three strategies are classified based on the choice of the parking orbits or the choice of the manifolds. Two strategies use butterfly and Halo orbits to park the vehicle before transferring to the target orbit. The third strategy, instead, uses manifolds to allow a direct phasing. In the paper, the three innovative strategies are designed and compare in a specific scenario.     

Genetic risks associated with radiation exposures during space flight.

The genetic risks associated with manned space flight are judged to be of little significance to the general population. The risks may be significant to the irradiated individual, particularly if one focuses attention on the incidence of dominant and chromosomal mutations that are expressed in the first generation offspring. Even so, the risk is not increased to a great extent by the low linear energy transfer (LET) component of the space radiations. It is the presumed high LET component, neutrons especially, that would make the major contribution to the risk, because the relative biological effectiveness (RBE) values for this component, relative to low dose-rate photon irradiation, are between 10 and 40, depending upon the particular genetic effect and dose-rate comparison. The appropriate RBE value would probably be 20 or greater, so that even small neutron doses become magnified in their contribution. Under the assumed condition of protracted exposure to 8 rads of low LET radiation and 2 rads of high LET radiation, or from 48 to 88 rem, the individual's risk of transmitting a new dominant mutation that will be expressed in his immediate offspring is estimated to increase by at least 4% and as much as about 40%. The HZE-particle component is not expected to make a significant contribution to the total risk.     

用一般脉宽调制器构造的变频式脉宽调制器

提出为直流变换器增加一个控制变量的概念,以弥补现行直流变换器只有一个调节变量引起的一些缺陷.本文选定的控制变量是直流变换器的运行频率f,将它与一次电源电压V联系起来,使f随V上升按预设的规律下降,以解决直流变换器以固定频率运行时这种情况下出现的许多问题,如开关管发热加剧等.实现这一设想的主要问题是将一般脉宽调制器构造成变频式脉宽调制器.现以脉宽调制器1842/3/4/5(简记为184X)和1842A/3A/4A/5A(简记为184XA)为例,给出了将它构造成变频式脉宽调制器的方法,示出了可实现的变频特性.设计了两个将一次电源电压转换为变频式脉宽调制器输入电压的线性转换电路,推导了计算这些电路中各元件参数的表达式.实验结果与设计一致.