Physical implications of discrepancy between summer and winter PC indices observed in the course of magnetospheric substorms

The PC index based on a statistically justified relationship between the polar cap magnetic activity and the interplanetary electric field E_KL has been derived as a value standardized for the E_KL intensity regardless of season, UT and hemisphere. As a result, the summer and winter PC indices are consistent with one another under ordinary conditions. Discrepancies between the summer and winter PC indices arising in the course of magnetospheric substorms are analyzed in this paper. It is argued that the channel of enhanced conductivity, formed in the auroral oval owing to intense auroral particle precipitation, strongly improves the conditions for closure of the Region 1 field-aligned currents in the winter dark polar region but only trivially affects the conditions of the Region 1 FAC closure in the summer sunlit ionosphere. Since the coefficients describing the relationship between E_KL and the polar cap magnetic activity were derived for statistically justified (i.e., mean) conditions, their application to such abnormal situation, as intense field-aligned currents in the winter dark polar region, leads to overestimation of the winter PC index. The summer and winter PC indices level off as soon as the intense auroral particle precipitation terminates and the auroral ionosphere in the winter and summer polar caps returns to the ordinary (statistically justified) state.     

Survival and death of the haloarchaeon Natronorubrum strain HG-1 in a simulated martian environment

Halophilic archaea are of interest to astrobiology due to their survival capabilities in desiccated and high salt environments. The detection of remnants of salty pools on Mars stimulated investigations into the response of haloarchaea to martian conditions. Natronorubrum sp. strain HG-1 is an extremely halophilic archaeon with unusual metabolic pathways, growing on acetate and stimulated by tetrathionate. We exposed Natronorubrum strain HG-1 to ultraviolet (UV) radiation, similar to levels currently prevalent on Mars. In addition, the effects of low temperature (4, −20, and −80 °C), desiccation, and exposure to a Mars soil analogue from the Atacama desert on the viability of Natronorubrum strain HG-1 cultures were investigated. The results show that Natronorubrum strain HG-1 cannot survive for more than several hours when exposed to UV radiation equivalent to that at the martian equator. Even when protected from UV radiation, viability is impaired by a combination of desiccation and low temperature. Desiccating Natronorubrum strain HG-1 cells when mixed with a Mars soil analogue impaired growth of the culture to below the detection limit. Overall, we conclude that Natronorubrum strain HG-1 cannot survive the environment currently present on Mars. Since other halophilic microorganisms were reported to survive simulated martian conditions, our results imply that survival capabilities are not necessarily shared between phylogenetically related species.     

Validation of B0 and B1 in the IRI 2001 model at low solar activity for Ilorin an equatorial station

A new set of data obtained at low solar activity from Ilorin, Nigeria (geog. latitude 8.5°N, geog longitude, 4.6°E, dip 4.1°S) is used to validate the IRI 2001 model at low solar activity. The results show in general a good agreement between model and observed B0 at night but an over estimation during daytime. The overestimation is greatest during the morning period (0600LT–1000LT). The model prediction for B1 is fairly good at night and during the day. A dependence of B0 on solar zenith angle χ is observed during the daytime. A formulation of the form B0 = A[cos(χ)ⁿ] is therefore proposed. Values of the constants n and A were determined for the period of low solar activity for this station.     

Molecular analysis of the graviperception signal transduction in the flagellate Euglena gracilis: Involvement of a transient receptor potential-like channel and a calmodulin

Euglena gracilis, a unicellular, photosynthetic flagellate is a model system for environmentally controlled behavior responses. The organism shows pronounced negative gravitaxis. This movement is based on physiological mechanisms, which in the past had been only indirectly assessed. It was shown that mechano-sensitive calcium channels are involved in the gravitaxis response. Recent studies have demonstrated that members of the transient receptor potential (TRP) family function as mechano-sensitive channels in several different cell types. We have sequenced part of a TRP gene in Euglena and applied RNA interference (RNAi) to confirm that these channels are involved in graviperception. It was found that RNAi against the putative TRP channel abolished gravitaxis. The genes of three calmodulins were sequences in Euglena, one of which was previously known in its protein structure (cal 1). The other two were unknown (cal 2 and cal 3). Cal 2 has been analyzed in detail. The biosynthesis of the corresponding proteins of cal 1 and cal 2 was inhibited by means of RNA interference to see whether this blockage impairs gravitaxis. RNAi of cal 1 leads to a long-term loss of free swimming in the cells (while euglenoid movement persists). It induced pronounced cell form aberrations and the division of cells was hampered. After recovery from RNAi the cell showed precise negative gravitaxis again. Thus cal 1 does not seem to be involved in gravitaxis. In contrast, the blockage of cal 2 has no pronounced influence on motility and cell form but leads to a complete loss of gravitactic orientation for more than 30 days showing that this calmodulin is an element in the signal transduction chain. The data are discussed in the context of the current model of the gravitaxis signal transduction chain in Euglena gracilis.     

Spaceflight results in increase of thick filament but not thin filament proteins in the paramyosin mutant of Caenorhabditis elegans

We have investigated the effect of microgravity during spaceflight on body-wall muscle fiber size and muscle proteins in the paramyosin mutant of Caenorhabditis elegans. Both mutant and wild-type strains were subjected to 10 days of microgravity during spaceflight and compared to ground control groups. No significant change in muscle fiber size or quantity of the protein was observed in wild-type worms; where as atrophy of body-wall muscle and an increase in thick filament proteins were observed in the paramyosin mutant unc-15(e73) animals after spaceflight. We conclude that the mutant with abnormal muscle responded to microgravity by increasing the total amount of muscle protein in order to compensate for the loss of muscle function.     

Effects of different carbon dioxide and LED lighting levels on the anti-oxidative capabilities of Gynura bicolor DC

Gynura bicolor DC is not only an edible plant but also a kind of traditional Chinese herbal medicine. G. bicolor DC grown in controlled environmental chambers under 3 CO₂ concentrations [450 (ambient), 1500 (elevated), 8000 (super-elevated) μmol mol⁻¹] and 3 LED lighting conditions [white (WL), 85% red + 15% blue (RB15), 70% red + 30% blue (RB30) ] were investigated to reveal plausible antioxidant anabolic responses to CO₂ enrichment and LED light quality. Under ambient and elevated CO₂ levels, blue light increasing from 15% to 30% was conducive to the accumulation of anthocyanins and total flavonoids, and the antioxidant activity of extract was also increased, but plant biomass was decreased. These results demonstrated that the reinforcement of blue light could induce more antioxidant of secondary metabolites, but depress the effective growth of G. bicolor DC under ambient and elevated CO₂ levels. In addition, compared with the ambient and elevated CO₂ levels, the increased anthocyanins, total flavonoids contents and antioxidant enzyme activities of G. bicolor DC under super-elevated CO₂ level could serve as important components of antioxidative defense mechanism against CO₂ stress. Hence, G. bicolor DC might have higher tolerance to CO₂ stress.     

Short-term forecasting regional model to predict M(3000)F2 over the European sector: Comparisons with the IRI model during moderate,disturbed, and very disturbed geomagnetic conditions

The hourly measurements of M(3000)F2 (M(3000)F2_meas) and the hourly quiet-time values of M(3000)F2 (M(3000)F2_QT) relative to the ionospheric observatories of Poitiers, Lannion, Dourbes, Slough, Rome, Juliusruh, Kaliningrad, Uppsala, Lyckesele, Sodankyla, and Kiruna as well as the hourly time-weighted accumulation series derived from the geomagnetic planetary index ap (ap(τ)), were considered during the period January 1957–December 2003 and used for the development of 11 short-term forecasting local models (STFLM) of M(3000)F2.     

Involvement of nitric oxide in the mechanism of biochemical alterations induced by simulated microgravity in Microcystis aeruginosa

Simulated microgravity (SMG) can inhibit proliferation and enhance microcystin production of Microcystis aeruginosa. We investigated the role of nitric oxide (NO) in regulating the SMG induced changes of proliferation, photochemical system II photochemical activity, pigment, soluble protein and microcystin production in M. aeruginosa. M. aeruginosa was exposed to 0.1 mM sodium nitroprusside (SNP, NO donor) or 0.02 mM 2-(4-carboxyphenyl)-4, 4, 5, 5-tetramethylimidazoline-1-oxyl-3-oxide (c-PTIO, NO scavenger) alone or in combination with SMG for 48 h. SMG and SNP inhibited the growth of M. aeruginosa while c-PTIO had no effect on cell number. As to yield, the negative effect of SMG was augmented by SNP and suppressed by c-PTIO. The intracellular concentrations of chlorophyll a, carotenoid, phycocyanin, soluble protein and microcystin were increased by SMG after 48 h. The effects of SMG on these metabolic processes could be enhanced by SNP and be partly eliminated by c-PTIO. Moreover, SNP and c-PTIO only functioned in these biochemical processes under SMG, unlike in the regulation of cell proliferation and yield. These results showed that the effects of SMG could be enhanced by adding exogenous NO and be mitigated by scavenging endogenous NO, revealing the involvement of NO in the changes in biochemistry processes induced by SMG in M. aeruginosa.     

Influence of the interaction between light intensity and CO2 concentration on productivity and quality of spinach (Spinacia oleracea L.) grown in fully controlled environment

The effects of the factorial combination of two light intensities (200 and 800 μmol m⁻² s⁻¹) and two CO₂ concentrations (360 and 800 ppm) were studied on the productivity and nutritional quality of spinach (Spinacia oleracea L.) grown under controlled environment. After 6 weeks within a growth chamber, spinach plants were sampled and analyzed for productivity and quality. There were no statistically significant interactions between the effects of light and CO₂ for all of the variables studied, except for the nitrate and oxalic acid content of the leaves. High light and high CO₂ independently one from the other, promoted spinach productivity, and the accumulation of ascorbic acid, while their interactive effect limited the accumulation of nitrate and oxalic acid in the spinach leaves. The results highlight the importance of considering the effects of the interaction among environmental variables on maximizing production and the nutritional quality of the food when cultivating and modeling the plant response in controlled environment systems such as for bioregenerative life support.     

The effects of CO2 on growth and transpiration of radish (Raphanus sativus) in hypobaria

Plants grown on long-term space missions will likely be grown in low pressure environments (i.e., hypobaria). However, in hypobaria the transpiration rates of plants can increase and may result in wilting if the water is not readily replaced. It is possible to reduce transpiration by increasing the partial pressure of CO₂ (pCO₂), but the effects of pCO₂ at high levels (>120 Pa) on the growth and transpiration of plants in hypobaria are not known. Therefore, the effects of pCO₂ on the growth and transpiration of radish (Raphanus sativus var. Cherry Bomb II) in hypobaria were studied. The fresh weight (FW), leaf area, dry weight (DW), CO₂ assimilation rates (C_A), dark respiration rates (DR), and transpiration rates from 26 day-old radish plants that were grown for an additional seven days at different total pressures (33, 66 or 101 kPa) and pCO₂ (40 Pa, 100 Pa and 180 Pa) were measured. In general, the dry weight of plants increased with CO₂ enrichment and with lower total pressure. In limiting pCO₂ (40 Pa) conditions, the transpiration for plants grown at 33 kPa was approximately twice that of controls (101 kPa total pressure with 40 Pa pCO₂). Increasing the pCO₂ from 40 Pa to 180 Pa reduced the transpiration rates for plants grown in hypobaria and in standard atmospheric pressures. However, for plants grown in hypobaria and high pCO₂ (180 Pa) leaf damage was evident. Radish growth can be enhanced and transpiration reduced in hypobaria by enriching the gas phase with CO₂ although at high levels leaf damage may occur.     

The effect of geocenter motion on Jason-2 orbits and the mean sea level

We compute a series of Jason-2 GPS and SLR/DORIS-based orbits using ITRF2005 and the std0905 standards ( Lemoine et al., 2010). Our GPS and SLR/DORIS orbit data sets span a period of 2 years from cycle 3 (July 2008) to cycle 74 (July 2010). We extract the Jason-2 orbit frame translational parameters per cycle by the means of a Helmert transformation between a set of reference orbits and a set of test orbits. We compare the annual terms of these time-series to the annual terms of two different geocenter motion models where biases and trends have been removed. Subsequently, we include the annual terms of the modeled geocenter motion as a degree-1 loading displacement correction to the GPS and SLR/DORIS tracking network of the POD process. Although the annual geocenter motion correction would reflect a stationary signal in time, under ideal conditions, the whole geocenter motion is a non-stationary process that includes secular trends. Our results suggest that our GSFC Jason-2 GPS-based orbits are closely tied to the center of mass (CM) of the Earth consistent with our current force modeling, whereas GSFC’s SLR/DORIS-based orbits are tied to the origin of ITRF2005, which is the center of figure (CF) for sub-secular scales. We quantify the GPS and SLR/DORIS orbit centering and how this impacts the orbit radial error over the globe, which is assimilated into mean sea level (MSL) error, from the omission of the annual term of the geocenter correction. We find that for the SLR/DORIS std0905 orbits, currently used by the oceanographic community, only the negligence of the annual term of the geocenter motion correction results in a – 4.67 ± 3.40 mm error in the Z-component of the orbit frame which creates 1.06 ± 2.66 mm of systematic error in the MSL estimates, mainly due to the uneven distribution of the oceans between the North and South hemisphere.     

Cowpeas and pinto beans: Performance and yields of candidate space crops in the laboratory biosphere closed ecological system

An experiment utilizing cowpeas (Vigna unguiculata L.), pinto beans (Phaseolus vulgaris L.) and Apogee ultra-dwarf wheat (Triticum sativa L.) was conducted in the soil-based closed ecological facility, Laboratory Biosphere, from February to May 2005. The lighting regime was 13 h light/11 h dark at a light intensity of 960 μmol m⁻² s⁻¹, 45 mol m⁻² day⁻¹ supplied by high-pressure sodium lamps. The pinto beans and cowpeas were grown at two different planting densities. Pinto bean production was 341.5 g dry seed m⁻² (5.42 g m⁻² day⁻¹) and 579.5 dry seed m⁻² (9.20 g m⁻² day⁻¹) at planted densities of 32.5 plants m⁻² and 37.5 plants m⁻², respectively. Cowpea yielded 187.9 g dry seed m⁻² (2.21 g m⁻² day⁻¹) and 348.8 dry seed m⁻² (4.10 g m⁻² day⁻¹) at planted densities of 20.8 plants m⁻² and 27.7 plants m⁻², respectively. The crop was grown at elevated atmospheric carbon dioxide levels, with levels ranging from 300–3000 ppm daily during the majority of the crop cycle. During early stages (first 10 days) of the crop, CO₂ was allowed to rise to 7860 ppm while soil respiration dominated, and then was brought down by plant photosynthesis. CO₂ was injected 27 times during days 29–71 to replenish CO₂ used by the crop during photosynthesis. Temperature regime was 24–28 °C day/deg 20–24 °C night. Pinto bean matured and was harvested 20 days earlier than is typical for this variety, while the cowpea, which had trouble establishing, took 25 days more for harvest than typical for this variety. Productivity and atmospheric dynamic results of these studies contribute toward the design of an envisioned ground-based test bed prototype Mars base.