Differentiation of cartilaginous anlagen in entire embryonic mouse limbs cultured in a rotating bioreactor.

Mechanisms involved in development of the embryonic limb have remained the same throughout eons of genetic and environmental evolution under Earth gravity (1 g). During the spaceflight era it has been of interest to explore the ancient theory that form of the skeleton develops in response to gravity, and that changes in gravitational forces can change the developmental pattern of the limb. This has been shown in vivo and in vitro, allowing the hypergravity of centrifugation and microgravity of space to be used as tools to increase our knowledge of limb development. In recapitulations of spaceflight experiments, premetatarsals were cultured in suspension in a bioreactor, and found to be shorter and less differentiated than those cultured in standard culture dishes. This study only measured length of the metatarsals, and did not account for possible changes due to the skeletal elements having a more in vivo 3D shape while in suspension vs. flattened tissues compressed by their own weight. A culture system with an outcome closer to in vivo and that supports growth of younger limb buds than traditional systems will allow studies of early Hox gene expression, and contribute to the understanding of very early stages of development. The purpose of the current experiment was to determine if entire limb buds could be cultured in the bioreactor, and to compare the growth and differentiation with that of culturing in a culture dish system. Fore and hind limbs from E11-E13 ICR mouse embryos were cultured for six days, either in the bioreactor or in center-well organ culture dishes, fixed, and embedded for histology. E13 specimens grown in culture dishes were flat, while bioreactor culture specimens had a more in vivo-like 3D limb shape. Sections showed excellent cartilage differentiation in both culture systems, with more cell maturation, and hypertrophy in the specimens cultured in the bioreactor. Younger limb buds fused together during culture, so an additional set of E11.5 limb buds was cultured with and without encapsulation in alginate prior to culturing in the bioreactor. Encapsulated limbs grown in the bioreactor did not fuse together, but developed only the more proximal elements while limbs grown in culture dishes formed proximal and distal elements. Alginate encapsulation may have reduced oxygenation to the progress zone of the developing limb bud resulting in lack of development of the more distal elements. These results show that the bioreactor supports growth and differentiation of skeletal elements in entire E13 limb buds, and that a method to culture younger limb buds without fusing together needs to be developed if any morphometric analysis is to be performed.     

Changes of deoxyribonucleoprotein in the spleen, thymus and liver of rats exposed to weightlessness and artificial gravity aboard the Cosmos biosatellites

Changes of deoxyribonucleoprotein in the spleen, thymus and liver of rats exposed to wegithlessness or artifical gravity on board biosatellites Cosmos 782 and Cosmos 936 after 20 days of flight were investigated. The level of polydeoxyribonucleotides in the spleen and thymus of rats exposed during the flight to weightlessness increased 4 – 11 hours after landing, suggesting breakdown of a part of the deoxyribonucleoprotein present. The use of artifical gravity prevented this breakdown in the thymus but not in the spleen. The breakdown was accompanied in the majority of cases by a decrease in teh deoxyribonucleoprotein content. We believe the breakdown of deoxyribonucleoprotein is due to a nonspecific stress reaction to the change from the weightless state to that of terrestrial gravity during landing. The polydeoxyribonucleotide level and amount of deoxyribonucleoprotein in the majority of cases returned to normal values during the 25 days of readaptation. No substantial change of deoxyribonucleoprotein was found in the liver. The different findings in the three organs are due to the fact that breakdown of deoxyribonucleoprotein takes place in sensitive cells underlying pycnosis. These cells are found in the spleen and thymus, but not in the liver.     

太阳风粘性效应的重新考察

利用Helios飞船对太阳风高速流的等离子体和磁场观测,本文考察了0.3-1AU作用在高速流上各种体积力的径向分布.结果表明:普遍采用的在碰撞为主等离子体中导出的经典粘性系数表式不适用于太阳风高速流;为使惯性力与各体积力之和相等,必须有大小跟这一空间范围内的太阳重力相近的粘性减速力.本文提出一种新的方法,导出了太阳风高速流中实际粘性力的径向分布,并求出了实际粘性系数的径向分布.结果发现,相对于经典粘性系数,1AU处太阳风高速流的粘性系数的减小不小于十分之一.      

Chondrogenesis in aggregates of embryonic limb cells grown in a rotating wall vessel.

Previous studies in this lab have shown that chondrogenesis is affected in growth plates of rats exposed to microgravity, and in micromass cultures of embryonic limb mesenchyme differentiating in space. In order to provide a three dimensional aspect not seen in the micromass system, and a tissue homogeneity not possible with explants of limb or limb elements, and to alleviate certain difficulties regarding crew time and stowage, we began culturing embryonic limb cells in Rotating Wall Vessels (RWV). First, these cells were attached to beads, and grown for up to 65 days in a type of RWV known as STLV at the Johnson Space Center. During this time, the cells and beads aggregated and the aggregates continued to increase in size, and differentiated into Alcian blue staining chondrocytes. Because our intent was to use these aggregates for implanting into bony defects in addition to their use in studies of chondrogenic regulation at 1g and microgravity, aggregates of these cells without beads were grown in the commercially available version of the STLV, and their ability to ossify when subcutaneously implanted assessed.     

Influence of gravity on the circadian timing system.

The circadian timing system (CTS) is responsible for daily temporal coordination of physiological and behavioral functions both internally and with the external environment. Experiments in altered gravitational environments have revealed changes in circadian rhythms of species ranging from fungi to primates. The altered gravitational environments examined included both the microgravity environment of spaceflight and hyperdynamic environments produced by centrifugation. Acute exposure to altered gravitational environments changed homeostatic parameters such as body temperature. These changes were time of day dependent. Exposure to gravitational alterations of relatively short duration produced changes in both the homeostatic level and the amplitude of circadian rhythms. Chronic exposure to a non-earth level of gravity resulted in changes in the period of the expressed rhythms as well as in the phase relationships between the rhythms and between the rhythms and the external environment. In addition, alterations in gravity appeared to act as a time cue for the CTS. Altered gravity also affected the sensitivity of the pacemaker to other aspects of the environment (i.e., light) and to shifts of time cues. Taken together, these studies lead to the conclusion that the CTS is indeed sensitive to gravity and its alterations. This finding has implications for both basic biology and space medicine.     

The mechanics of air-breathing in anuran larvae: implications to the development of amphibians in microgravity.

Because of their rapid development, amphibians have been important model organisms in studies of how microgravity affects vertebrate growth and differentiation. Both urodele (salamanders) and anuran (frogs and toads) embryos have been raised in orbital flight, the latter several times. The most commonly reported and striking effects of microgravity on tadpoles are not in the vestibular system, as one might suppose, but in their lungs and tails. Pathological changes in these organs disrupt behavior and retard larval growth. What causes malformed (typically lordotic) tadpoles in microgravity is not known, nor have axial pathologies been reported in every flight experiment. Lung pathology, however, has been consistently observed and is understood to result from the failure of the animals to inflate their lungs in a timely and adequate fashion. We suggest that malformities in the axial skeleton of tadpoles raised in microgravity are secondary to problems in respiratory function. We have used high speed videography to investigate how tadpoles breathe air in the 1G environment. The video images reveal alternative species-specific mechanisms, that allow tadpoles to separate air from water in less that 150 ms. We observed nothing in the biomechanics of air-breathing in 1G that would preclude these same mechanisms from working in microgravity. Thus our kinematic results suggest that the failure of tadpoles to inflate their lungs properly in microgravity is due to the tadpoles' inability to locate the air-water interface and not a problem with the inhalation mechanism per se.     

投放激光制导炸弹的战术研究

对攻击机本机照射激光束、投放激光制导炸弹的战术进行研究,即攻击机本机照射激光束、采用机翼非水平转弯投放方式投放激光制导炸弹、转弯退出的战术.其关键点在于攻击机采用转弯退出战术把激光束连续地照射目标的战术要求和退出意图二者融合.该战术由于采用机动投放和曲线退出战术方式,能够大大提高攻击机的生存力.采用简化的攻击机质点模型,通过对攻击机转弯退出过程的分析,给出采用转弯退出战术的具体条件.投放激光制导炸弹任务过程的数学仿真结果表明,所给出的条件能够满足攻击机本机照射激光束、投放激光制导炸弹、转弯退出的战术要求.      

基于双力源的叠加式多维力传感器校准装置研究CSCD

介绍了一种基于叠加机原理的双力源多维力传感器校准装置的设计与研制,对装置的测量不确定度进行了分析,通过对三分量力传感器的校准实验,证明了校准装置不仅可以对多分量力传感器进行单分量校准,还能方便地实现耦合误差的校准。     

Long term effects of low doses of 56Fe ions on the brain and retina of the mouse: ultrastructural and behavioral studies.

Eight month old male C57BL6 mice were exposed without anesthesia to whole-body irradiation in circular holders. The mice were tested for behavioral decrements after 0.5 and 50 rads of Fe particle irradiation at 6 and 12 months post irradiation to obtain long term results. A standard maze was used and the animals were timed for completion thereof. A string test also was administered to the mice, testing their ability to grasp and move along a string to safety. The results from animals exposed to 50 rads were significantly different from [correction of fron] control results to p = < .001 in both systems of testing. The hippocampus (believed to be the location of environmental interaction in the brain) and the retina were examined for ultrastructural changes. The ultrastructural changes were similar to those we found in our Cosmos 782, 936 and in our Argon experiments. The mouse data indicate that iron particles were able to induce long term changes in the central nervous system which lead to behavioral impairment.     

Organization of cytoskeleton during differentiation of gravisensitive root sites under clinorotation.

Key role in cell gravisensing is attributed to the actin cytoskeleton which acts as a mediator in signaling reactions, including graviperception. Despite of increased attention to the actin cytoskeleton, major gaps in our understanding of its functioning in plant gravisensing still remain. To fill these gaps, we propose a novel approach focused on the investigation of actin involvement in the development of columella cells and cells in the transition zone of roots submitted to clinorotation. Both statocytes and cells in the transition zone represent the postmitotic cells which take origin in root meristems and are specified into graviperceptive (root cap) and gravireacting (transition zone) root tissues. The aim of the research was to investigate and compare the microfilament arrangements in root cap statocytes and peripheral root tissues (epidermis and cortex cells) in the transition zone and to find out how the actin cytoskeleton is involved in their specification under clinostat conditions. So far, our experiments have shown that under clinorotation the cytoplasmic microfilament network in the cortex cells in the transition zone is significantly enhanced. It is suggested that more abundant cytoplasmic microfilaments could strengthen the cortical actin cytoskeleton arranged parallel with the cortical microtubules, which are found to be partially disorganized in this area. Due to microtubule disorganization, the functioning of cellulose-synthesizing machinery and proper deposition of cell wall might be affected and could cause the alterations in the growth mode. But, in our case growth of the cells in the transition zone under clinorotation was rather stable. Due to our opinion, general stability of cell growth under clinorotation is promoted by mutual functional interrelation between actin and tubulin cytoskeletons. It is suggested that a strengthened cortical actin cytoskeleton restricts the cell growth instead of disorganized microtubules.     

Improvements in the re-flight of spaceflight experiments on plant tropisms

In order to effectively study phototropism, the directed growth in response to light, we performed a series of experiments in microgravity to better understand light response without the “complications” of a 1-g stimulus. These experiments were named TROPI (for tropisms) and were performed on the European Modular Cultivation System (EMCS), a laboratory facility on the International Space Station (ISS). TROPI-1 was performed in 2006, and while it was a successful experiment, there were a number of technical difficulties. We had the opportunity to perform TROPI-2 in 2010 and were able to optimize experimental conditions as well as to extend the studies of phototropism to fractional gravity created by the EMCS centrifuge. This paper focuses on how the technical improvements in TROPI-2 allowed for a better experiment with increased scientific return. Major modifications in TROPI-2 compared to TROPI-1 included the use of spaceflight hardware that was off-gassed for a longer period and reduced seed storage (less than 2 months) in hardware. These changes resulted in increased seed germination and more vigorous growth of seedlings. While phototropism in response to red illumination was observed in hypocotyls of seedlings grown in microgravity during TROPI-1, there was a greater magnitude of red-light-based phototropic curvature in TROPI-2. Direct downlinking of digital images from the ISS in TROPI-2, rather than the use of analog tapes in TROPI-1, resulted in better quality images and simplified data analyses. In TROPI-2, improved cryo-procedures and the use of the GLACIER freezer during transport of samples back to Earth maintained the low temperature necessary to obtain good-quality RNA required for use in gene profiling studies.     

The ASTROCULTURE(TM) flight experiment series, validating technologies for growing plants in space.

A flight experiment, ASTROCULTURE(TM)-1 (ASC-1), to evaluate the operational characteristics and hardware performance of a porous tube nutrient delivery system (PTNDS) was flown on STS-50 as part of the U.S. Microgravity Laboratory-1 mission, 25 June to 9 July, 1992. This experiment is the first in a series of planned ASTROCULTURE(TM) flights to validate the performance of subsystems required to grow plants in microgravity environments. Results indicated that the PTNDS was capable of supplying water and nutrients to plants in microgravity and that its performance was similar in microgravity to that in 1g on Earth. The data demonstrated that water transfer rates through a rooting matrix are a function of pore size of the tubes, the degree of negative pressure on the 'supply' fluid, and the pressure differential between the 'supply' and 'recovery' fluid loops. A slightly greater transfer rate was seen in microgravity than in 1g, but differences were likely related to the presence of hydrostatic pressure effects at 1g. Thus, this system can be used to support plant growth in microgravity or in partial gravity as on a lunar or Mars base. Additional subsystems to be evaluated in the ASTROCULTURE(TM) flight series of experiments include lighting, humidity control and condensate recovery, temperature control, nutrient composition control, CO2 and O2 control, and gaseous contaminant control.     

航空发动机空中停车率的优化

提出了民用航空发动机空中停车率与维修成本控制的优化决策方法.以空中停车损失期望作为间接营运成本指标,以维修成本与间接营运成本之和最小为优化目标,建立了确定最优空中停车率的数学规划方法,并给出了空中停车率与维修成本优化控制的简化方法.本文所提供的优化决策方法对航空公司进行在翼航空发动机的任务可靠性水平与维修费用控制决策有参考价值.     

Biologically weighted measurement of UV radiation in space and on Earth with the biofilm technique.

Biological dosimetry has provided experimental proof of the high sensitivity of the biologically effective UVB doses to changes in atmospheric ozone and has thereby confirmed the predictions from model calculations. The biological UV dosimeter 'biofilm' whose sensitivity is based on dried spores of B. subtilis as UV target weights the incident UV radiation according to its DNA damaging potential. Biofilm dosimetry was applicated in space experiments as well as in use in remote areas on Earth. Examples are long-term UV measurements in Antarctica, measurements of diurnal UV profiles parallel in time at different locations in Europe, continuous UV measurements in the frame of the German UV measurement network and personal UV dosimetry. In space biofilms were used to determine the biological efficiency of the extraterrestrial solar UV, to simulate the effects of decreasing ozone concentrations and to determine the interaction of UVB and vitamin D production of cosmonauts in the MIR station.     

On wavelet techniques in atmospheric sciences

Wavelet analysis has been formalized extensively due to the efforts of mathematicians, physicists and engineers in the last two decades. It has generated a tremendous interest in these communities both in theoretical and applied areas, in such a way that wavelet analysis is also considered now as a nucleus of shared aspirations and ideas. Initially applied to seismic signal studies in geophysics in the 1980s, wavelet techniques have been explored in the atmospheric sciences since the pioneer applications in turbulence studies. If one decides to apply the wavelet analysis to a given signal, it is worthwhile to assess the actual need of the technique itself and the best way to perform it. In atmospheric signal applications, two main directions have been followed: the singularity and the variance analysis. In this paper, the potential uses of this tool supported by some recently published works in the field of atmospheric sciences are discussed. Therefore, initially the characteristics and main properties of the wavelet analysis are presented, focusing on those that are mostly used in the analysis of atmospheric signals. Continuous and discrete wavelet transforms are also discussed, as well as the scalograms and the variance analysis. Finally, some examples of wavelet analysis applied to a wide range of atmospheric science phenomena are presented.     

Predictive fault-tolerant control of an all-thruster satellite in 6-DOF motion via neural network model updating

The problem of controlling an all-thruster spacecraft in the coupled translational-rotational motion in presence of actuators fault and/or failure is investigated in this paper. The nonlinear model predictive control approach is used because of its ability to predict the future behavior of the system. The fault/failure of the thrusters changes the mapping between the commanded forces to the thrusters and actual force/torque generated by the thruster system. Thus, the basic six degree-of-freedom kinetic equations are separated from this mapping and a set of neural networks are trained off-line to learn the kinetic equations. Then, two neural networks are attached to these trained networks in order to learn the thruster commands to force/torque mappings on-line. Different off-nominal conditions are modeled so that neural networks can detect any failure and fault, including scale factor and misalignment of thrusters. A simple model of the spacecraft relative motion is used in MPC to decrease the computational burden. However, a precise model by the means of orbit propagation including different types of perturbation is utilized to evaluate the usefulness of the proposed approach in actual conditions. The numerical simulation shows that this method can successfully control the all-thruster spacecraft with ON-OFF thrusters in different combinations of thruster fault and/or failure.     

半球谐振子疲劳寿命分析与仿真

半球谐振子的寿命和损伤是直接影响高精度半球谐振陀螺使用时长和安全性的重要因素。目前国内加工的半球谐振子所用的熔融石英玻璃材料主要依靠进口,采用传统的疲劳寿命实验确定方法成本过于昂贵,因此需要利用疲劳分析软件对半球谐振子的疲劳寿命进行分析。文章通过半球谐振陀螺应力分析,采用ANSYS软件对熔融石英半球谐振子进行应力分析仿真,确定因残余应力所引起的疲劳危险部位,并在疲劳部位进行裂纹扩展分析,得到 、 和 型应力强度因子,以使在半球谐振子结构设计和使用过程中对易疲劳的部位进行有效监控和预防。     

The effects of heavy particle irradiation on exploration and response to environmental change.

Free radicals produced by exposure to heavy particles have been found to produce motor and cognitive behavioral toxicity effects in rats similar to those found during aging. The present research was designed to investigate the effects of exposure to 56Fe particles on the ability of male Sprague-Dawley rats to detect novel arrangements in a given environment. Using a test of spatial memory previously demonstrated to be sensitive to aging, open field activity and reaction to spatial and non-spatial changes were measured in a group that received a dose of 1.5 Gy (n=10) of 56Fe heavy particle radiation or in non-radiated controls (n=10). Animals irradiated with 1.5 Gy of 56Fe particles exhibited some age-like effects in rats tested, even though they were, for the most part, subtle. Animals took longer to enter, visited less and spent significantly less time in the middle and the center portions of the open field, independently of total frequency and duration of activity of both groups. Likewise, irradiated subjects spend significantly more time exploring novel objects placed in the open field than did controls. However, irradiated subjects did not vary from controls in their exploration patterns when objects in the open field were spatially rearranged. Thus, irradiation with a dose of 1.5 Gy of 56Fe high-energy particle radiation elicited age-like effects in general open field exploratory behavior, but did not elicit age-like effects during the spatial and non-spatial rearrangement tasks.     

面向网络化制造的数控车间组织模式

分析了网络化制造的基本特征和内涵,提出了面向企业的网络化制造系统运行模式;在对某大型航空制造企业的数控车间调研与应用实施的基础上,针对其组织模式存在问题,采用分行企业和独立制造岛的思想建立了适合数控车间网络化、敏捷化制造的分形组织模型;针对车间原有生产模式、产品特性,提出了基于成组技术的数控车间生产单元和生产线的划分方法,建立了面向产品流程的机构组成规则和车间内部的服务支援规则.该模型、方法、原则对传统的离散制造企业具有普遍的适用性,现已成功地被该数控车间采纳.