Effects of Microgravity on Human Physiology and Ground-Based Models for Space Physiological Studies

With the development of manned spaceflight, more and more researches are involved in the area of gravitation physiology. When astronauts are exposed to microgravity, a series of special physiological or pathological changes will occur, which will start self-regulation mechanisms to reduce abnormalities and help the organism to better adapt to microgravity. However, these adaptive changes may also induce degradation or damage to physiological functions. This paper summarizes the physiological effects of microgravity on the human body from the aspects of skeletal and mineral metabolism, muscle structure and function, vestibular functions, cardiovascular function and pulmonary function, as well as expounds some commonly used ground-based space analogies. The paper will provide a reference for further study on the physiological effects of microgravity.     

A mathematical and experimental simulation of the hematological response to weightlessness

Two ground-based methods of weightlessness simulation--a computer model of erythropoiesis feedback regulation and bedrest--were used to investigate the mechanisms which lead to loss of red cell mass during spaceflight. Both methods were used to simulate the first Skylab mission of 28 days. Human bedrest subjects lose red cell mass linearly with time and in this study the loss was 6.7% at the end of four weeks (compared to 14% in Skylab). Postbedrest recovery of red cell mass was delayed for two weeks during which time a further decline in this quantity was noted. This is consistent with the first Skylab mission but not with the two longer flights of two and three months. Hemoconcentration, observed early in the study, was essentially maintained despite red cell loss because of continued loss of plasma volume. The computer model, using the time-varying hematocrit data to estimate red cell production rates, predicted dynamic behavior of plasma volume and red cell mass that was in close agreement with the measured values. The results support the hypothesis that red cell loss during supine bedrest is a normal physiological feedback process in response to hemoconcentration enhanced tissue oxygenation and suppression of red cell production. In contrast, the delayed postbedrest recovery of red cell mass was more difficult to explain, especially in the light of enhanced reticulocyte indices observed at the onset on ambulation. Model simulation suggested the possibilities, still to be experimentally demonstrated, that this period was marked by some combination of increased oxygen-hemoglobin affinity, small reductions in mean red cell life span, ineffective erythropoiesis, or abnormal reticulocytosis. The question of whether hemoconcentration is the sole contributor to spaceflight red cell losses also remains to be resolved.     

Fluid volumes changes induced by spaceflight

The blood volume (BV), plasma volume (PV), and extracellular fluid volume changes produced in crewmembers during spaceflights of 11-84 days were compared to changes after 14 or 28 days of bedrest. Spaceflight and bedrest produce approximately equal BV changes but the recorded PV change after spaceflight was less. However, the diurnal change in PV may explain the smaller decreases recorded after spaceflight. The cardiovascular deconditioning caused by spaceflight and bedrest was compared using the mean heart rate response to lower body negative pressure (LBNP) testing at -50 mmHg pressure. These tests show approximately equal LBNP produced heart rate changes after bedrest and spaceflight. A countermeasure which includes 4 hr of LBNP treatment at -30 mmHg and the ingestion of one l. of saline was studied and found capable of returning the heart rate response and the PV of bedrested subjects to control (prebedrest) levels suggesting that it would be useful to the crewmembers after a spaceflight.     

Effects of prolonged space flight on rat skeletal muscle

The effect of a 20-day space flight on water, Na+, K+, Mg2+, Ca2+ and glycogen contents as well as on activities of glycogen metabolism enzymes--glycogen synthetase and glycogen phosphorylase--of rat skeletal muscles was studied. This data is regarded as an integral test characterizing the state of contractile tissue of the animals at the final stage of flight aboard biosatellites. The measurements indicate that there were no significant changes of cations and glycogen contents nor of the enzymic activities in fast-twitch muscles during the 20-day spaceflight. At the same time dehydration in these muscles was observed, which disappeared on the 25th postflight day. In slow-twitch antigravitational skeletal muscle (m. soleus) there was a decrease of K+ and increase of Na+ in the tissue contents. The changes disappeared at the end of the on-earth readaptation period. From the pattern of these observations, we can conclude that the 20-day space flight leads to some reversible biochemical changes of the rat skeletal muscles. A conclusion can be drawn about necessity of creating, aboard the spaceship, an artificial load on antigravitational skeletal muscles.     

Respective effects of hindlimb suspension, confinement and spaceflight on myotendinous junction ultrastructure

This study compares the effects of 14-day confinement and spaceflight with the respective effects of 8, 18 and 29-day hindlimb suspension on rat soleus and plantaris MTJ ultrastructure. Independently of the experimental situation, greater morphological changes were observed in the soleus as compared to the plantaris MTJ. 18 days of suspension and 14 days of confinement resulted in ultrastructural modifications of the digit-like processes in the soleus MTJ. Additional changes were observed in the myofibrils, microtendon and tendon after 29 days of suspension and 4 days of spaceflight. These results emphasize the influence of the intensity and duration of the muscle loading on the MTJ ultrastructure.     

Caenorhabditis elegans survives atmospheric breakup of STS-107, space shuttle Columbia

The nematode Caenorhabditis elegans, a popular organism for biological studies, is being developed as a model system for space biology. The chemically defined liquid medium, C. elegans Maintenance Medium (CeMM), allows axenic cultivation and automation of experiments that are critical for spaceflight research. To validate CeMM for use during spaceflight, we grew animals using CeMM and standard laboratory conditions onboard STS-107, space shuttle Columbia. Tragically, the Columbia was destroyed while reentering the Earth's atmosphere. During the massive recovery effort, hardware that contained our experiment was found. Live animals were observed in four of the five recovered canisters, which had survived on both types of media. These data demonstrate that CeMM is capable of supporting C. elegans during spaceflight. They also demonstrate that animals can survive a relatively unprotected reentry into the Earth's atmosphere, which has implications with regard to the packaging of living material during space flight, planetary protection, and the interplanetary transfer of life.     

The role of HZE particles in space flight: results from spaceflight and ground-based experiments.

Selected results from experiments investigating the potentially specific radiobiological importance of the cosmic HZE (= high Z, energetic) particles are discussed. Results from the Biostack space flight experiments, which were designed to meet the experimental requirements imposed by the microdosimetric nature of this radiation field, clearly indicate the existence of radiation mechanisms which become effective only at higher values of LET (linear energy transfer). Accelerator irradiation studies are reviewed which conform with this conjecture. The recently discovered production of "micro-lesions" in mammalian tissues by single HZE particles is possibly the most direct evidence. Open questions concerning the establishment of radiation standards for manned spaceflight, such as late effects, interaction with flight dynamic parameters, and weightlessness, are indicated.     

失重模拟技术的发展及其比较研究

失重或称微重力环境是载人航天轨道飞行中的重要环境因素,地面上失重模拟实验是航天前的重要准备工作之一。失重模拟方法及其设备多种多样,要依其目的不同而进行选择。文章对上述问题进行了充分的比较研究,并在此基础上指出了中性浮力水槽和失重飞机是失重模拟设备中最常用和最有效的工具。建议从我国的实际情况出发,适时的加以建造。     

Skill maintenance in extended spaceflight: a human factors analysis of space and analogue work environments

This paper discusses the implications of increasing mission lengths of manned spaceflight for the design of future space systems from a human factors point of view. It is argued that the increase in mission duration has brought about a number of new problems, which have not been sufficiently addressed in space research. Therefore, a review of analogue work environments is carried out to make up for the paucity of space research found in the area of human performance in long-duration spaceflight. This resulted in an evaluation of seven analogue environments concerning their similarity to space with industrial process control and nuclear submarines coming out as the closest match on the technical dimension. Finally, some recommendations are given from the lessons learned in spaceflight, simulation studies and appropriate analogue environments.     

The ankle ergometer: a new tool for quantifying changes in mechanical properties of human muscle as a result of spaceflight

A mechanical device for studying changes in mechanical properties of human muscle as a result of spaceflight is presented. Its main capacities are to allow during a given experiment investigation of both contractile and visco-elastic properties of a musculo-articular complex using respectively isometric contractions, isokinetic movements, quick-release tests and sinusoidal perturbations. This device is a motor driven ergometer associated to an experimental protocol designed for pre- and post-flight experiments. As microgravity preferentially affects postural muscles, the apparatus was designed to test muscle groups crossing the ankle joint. Three subjects were tested during the Euromir '94 mission. Preliminary results obtained on the european astronaut are briefly reported. During the next two years the experiments will be performed during six missions.     

Spaceflight and clinorotation cause cytoskeleton and mitochondria changes and increases in apoptosis in cultured cells.

The cytoskeleton is a complex network of fibers that is sensitive to environmental factors including microgravity and altered gravitational forces. Cellular functions such as transport of cell organelles depend on cytoskeletal integrity; regulation of cytoskeletal activity plays a role in cell maintenance, cell division, and apoptosis. Here we report cytoskeletal and mitochondria alterations in cultured human lymphocyte (Jurkat) cells after exposure to spaceflight and in insect cells of Drosophila melanogaster (Schneider S-1) after exposure to conditions created by clinostat rotation. Jurkat cells were flown on the space shuttle in Biorack cassettes while Schneider S-1 cells were exposed to altered gravity forces as produced by clinostat rotation. The effects of both treatments were similar in the different cell types. Fifty percent of cells displayed effects on the microtubule network in both cell lines. Under these experimental conditions mitochondria clustering and morphological alterations of mitochondrial cristae was observed to various degrees after 4 and 48 hours of culture. Jurkat cells underwent cell divisions during exposure to spaceflight but a large number of apoptotic cells was also observed. Similar results were obtained in Schneider S-1 cells cultured under clinostat rotation. Both cell lines displayed mitochondria abnormalities and mitochondria clustering toward one side of the cells which is interpreted to be the result of microtubule disruption and failure of mitochondria transport along microtubules. The number of mitochondria was increased in cells exposed to altered gravity while cristae morphology was severely affected indicating altered mitochondria function. These results show that spaceflight as well as altered gravity produced by clinostat rotation affects microtubule and mitochondria organization and results in increases in apoptosis. Grant numbers: NAG 10-0224, NAG2-985.     

Spatial factors and muscle spindle input influence the generation of neuromuscular responses to stimulation of the human foot

Removal of the mechanical pressure gradient on the soles leads to physiological adaptations that ultimately result in neuromotor degradation during spaceflight. We propose that mechanical stimulation of the soles serves to partially restore the afference associated with bipedal loading and assists in attenuating the negative neuromotor consequences of spaceflight. A dynamic foot stimulus device was used to stimulate the soles in a variety of conditions with different stimulation locations, stimulation patterns and muscle spindle input. Surface electromyography revealed the lateral side of the sole elicited the greatest neuromuscular response in ankle musculature, followed by the medial side, then the heel. These responses were modified by preceding stimulation. Neuromuscular responses were also influenced by the level of muscle spindle input. These results provide important information that can be used to guide the development of a “passive” countermeasure that relies on sole stimulation and can supplement existing exercise protocols during spaceflight.     

Redefining safety in commercial space: Understanding debates over the safety of private human spaceflight initiatives in the United States

In 2009 President Obama proposed a budget for the National Aeronautics and Space Administration (NASA) that canceled the Constellation program and included the development of commercial crew transportation systems into low Earth orbit. This significant move to shift human spaceflight into the private sector sparked political debate, but much of the discourse has focused on impacts to “safety.” Although no one disputes the importance of keeping astronauts safe, strategies for defining safety reveal contrasting visions for the space program and opposing values regarding the privatization of U.S. space exploration. In other words, the debate over commercial control has largely become encoded in arguments over safety. Specifically, proponents of using commercial options for transporting astronauts to the International Space Station (ISS) argue that commercial vehicles would be safe for astronauts, while proponents of NASA control argue that commercial vehicles would be unsafe, or at least not as safe as NASA vehicles. The cost of the spaceflight program, the technical requirements for designing a vehicle, the track record of the launch vehicle, and the experience of the launch provider are all incorporated into what defines safety in human spaceflight. This paper analyzes these contested criteria through conceptual lenses provided by fields of science and technology policy (STP) and science, technology, and society (STS). We ultimately contend that these differences in definition result not merely from ambiguous understandings of safety, but from intentional and strategic choices guided by normative positions on the commercialization of human spaceflight. The debate over safety is better considered a proxy debate for the partisan preferences embedded within the dispute over public or private spaceflight.     

Vascular functions in humans following cardiovascular adaptations to spaceflight

Purpose: Diminished vascular function is a primary cardiovascular risk of spaceflight identified in the 2004 NASA Bioastronautics Critical Path Roadmap based on: (1) structural and functional alterations in arterial vessels of animals undergoing hindlimb unloading and; (2) lower peripheral vascular resistance (PVR) in astronauts who became presyncopal after spaceflight.Methods: We conducted a critical review of published data obtained from spaceflight and relevant ground-based microgravity simulations in an effort to interpret the meaning of altered responses in PVR and their relationship to postflight presyncope.Results: Presyncope reported in astronauts on landing day was associated with lower peripheral resistance. However, non-presyncopal astronauts demonstrated significantly elevated vascular resistance in the upright posture after compared with before spaceflight. Results from both space and ground experiments suggest that preflight maximal vasoconstrictor capacity is inherently lower in presyncopal astronauts, but unaltered by spaceflight.Conclusions: Vasoconstrictor reserve is associated with lower blood volume adaptation to microgravity. Rather than reduced vascular function, low inherent maximal vasoconstrictor capacity and reduced vasoconstrictor reserve secondary to decreased circulating vascular volume explain lower peripheral vascular resistance in astronauts who experience presyncopal episodes on landing day.     

The use of micro-worlds for human factors research in extended spaceflight.

This article provides an analysis of the utility of micro-worlds for human factors research in extended spaceflight. It outlines potential areas of application of the micro-world research paradigm in the context of spaceflight. While the research literature provides several examples of micro-worlds that simulate different work environments of high complexity, little work has been done with this tool in the operational environment of spaceflight. The author presents a micro-world, called CAMS, that has been especially developed for use in space-related research. The results from a research programme (comprising seven studies using CAMS) are presented. Based on a comparative analysis of the benefits and limitations of micro-worlds compared to other simulation types, the author argues that micro-worlds can be a very effective tool, provided their inherent limitations are taken into consideration.     

How the science and engineering of spaceflight contribute to understanding the plasticity of spinal cord injury.

Space programs support experimental investigations related to the unique environment of space and to the technological developments from many disciplines of both science and engineering that contribute to space studies. Furthermore, interactions between scientists, engineers and administrators, that are necessary for the success of any science mission in space, promote interdiscipline communication, understanding and interests which extend well beyond a specific mission. NASA-catalyzed collaborations have benefited the spinal cord rehabilitation program at UCLA in fundamental science and in the application of expertise and technologies originally developed for the space program. Examples of these benefits include: (1) better understanding of the role of load in maintaining healthy muscle and motor function, resulting in a spinal cord injury (SCI) rehabilitation program based on muscle/limb loading; (2) investigation of a potentially novel growth factor affected by spaceflight which may help regulate muscle mass; (3) development of implantable sensors, electronics and software to monitor and analyze long-term muscle activity in unrestrained subjects; (4) development of hardware to assist therapies applied to SCI patients; and (5) development of computer models to simulate stepping which will be used to investigate the effects of neurological deficits (muscle weakness or inappropriate activation) and to evaluate therapies to correct these deficiencies.     

In vitro edible muscle protein production system (MPPS): stage 1, fish

The working efficiency and state-of-mind of a Space vehicle crew on long-term missions is dependent on the suitability of living conditions including food. Our purpose was to establish the feasibility of an in vitro muscle protein production system (MPPS) for the fabrication of surrogate muscle protein constructs as food products for Space travelers. In the experimental treatments, we cultivated the adult dorsal abdominal skeletal muscle mass of Carassius (Gold fish). An ATCC fish fibroblast cell line was used for tissue engineering investigations. No antibiotics were used during any phase of the research. Our four treatments produced these results: a low contamination rate, self-healing, cell proliferation, a tissue engineered construct of non-homologous co-cultured cells with explants, an increase in tissue size in homologous co-cultures of explants with crude cell mixtures, maintenance of explants in media containing fetal bovine serum substitutes, and harvested explants which resembled fresh fish filets.

We feel that not only have we pointed the way to an innovative, viable means of supplying safe, healthy, nutritious food to Space voyagers on long journeys, but our research also points the way to means of alleviating food supply and safety problems in both the public and private sectors worldwide.     

航天任务飞行控制分析系统的设计和应用

介绍了一个基于windows环境下的多任务航天任务飞行控制分析系统的设计以及实际应用,该系统为任务分析人员、指挥决策人员在任务准备、实施中快速灵活地进行各种复杂条件下的航天任务分析提供了可视化的操作平台以及全面的图表和文字报告。系统采用数据库技术,为用户提供了各种航天器参数和测站参数等信息。文中介绍了该系统的软件结构、数学模型和功能设计,并给出了具体的仿真应用。     

载人飞船泄复压过程中轨道舱的噪声环境试验研究

为了考察航天员对载人飞船泄复压过程中轨道舱内噪声环境的适应性,在KM6水平舱进行了相应的模拟试验,对轨道舱中的噪声环境进行了试验测量与研究.试验结果及航天员的实际感受表明,泄复压过程中轨道舱内的噪声环境满足要求,产生的噪声对航天员不会造成伤害.