Solid-state integrating detectors as an indicator of biological doses from HZE particles.

For interpretation of results obtained in future biological experiments in the International Space Station (ISS), biologically equivalent doses have to be determined using small-scale detectors without disturbing the surrounding radiation field. The detectors should be lightweight, stable, safe, and simple in handling. Solid-state integrating detectors (SSID) can satisfy these requirements. This paper demonstrates that combination of SSID such as thermoluminescence dosimeters and radiophotoluminescence glasses can be practically used for the evaluation of biologically equivalent doses. Statistical errors (type-A uncertainty) of this method will be satisfactorily small relative to those generally observed in biological responses. Permissible levels of systematic errors (type-B uncertainty) depend on dosimetry purposes (most-probable or conventional) and variability of biological responses.     

Closed Artificial ecosystems as a means of ecosystem studies for Earth and space needs.

Closed Artificial ecosystems (CAES) have good prospects for wide use as new means for quantitative studies of different types of both natural ecosystems and man-made ones. The paper deals with the discussion of three points of CAES applications. The first one is of importance for theoretical ecology development and is connected with bringing together "holistic" and "merological" approaches in ecosystems studies. Using CAES, we can combine both approaches, taking into account the biotic turnover of limiting substrates which few in number even for complicated natural ecosystems. The second CAES use concerns the development of "ecosystems health" concept and application of a key-factor-approach for the indication and measurement of healthy unhealthy state and functioning of ecosystems or their links. The third use is more of an applied nature, oriented to the intensification of bioremediation or biodepollution processes in different types of ecosystems, including the global biosphere. Grant numbers: N 99-04-96017, N25.     

Catalase activity as a potential indicator of the reducer component of small closed ecosystems.

Dynamics of catalase activity has been shown to reflect the growth curve of microorganisms in batch cultivation (celluloselythic bacteria Bacillus acidocaldarius and bacteria of the associated microflora Chlorella vulgaris). Gas and substrate closure of the three component ecosystems with spatially separated components "producer-consumer-reducer" (Chl. vulgaris-Paramecium caudatum-B. acidocaldarius, two bacterial strains isolated from the associated microflora Chl. vulgaris) demonstrated that the functioning of the reducer component can be estimated by the catalase activity of mciroorganisms of this component.     

The use of microcosms as an experimental approach to understanding terrestrial ecosystem functioning.

Since 1986, a series of microcosm experiments has been conducted at the Unit of Comparative Plant Ecology (UCPE) in an attempt to test our understanding of the principles controlling the structure and dynamics of plant communities and ecosystems. In each experiment microcosms have been seeded with a common pool of organisms, and systems have been allowed to assemble under replicated controlled conditions. Experiment variables have included mineral nutrient supply, temperature, moisture supply, soil depth, carbon dioxide concentration, mycorrhizas, rhizobia, herbivores and carnivores. Results from these experiments are presented to illustrate the value of synthesised ecosystems in ecological research.     

On monitoring the bacterial component as an indicator of the state of small man-made ecosystems.

High reproduction rates make the bacterial component of ecosystems a good indicator of the state of the system on the whole. This determines the necessity to develop rapid monitoring of the functional state of the bacterial component of small ecosystems. Information about substrate concentration in the population is indicative of the state of the bacterial culture. Conventional methods of monitoring the concentration of integral substrate in the system take time much longer than the changes in the ecosystem. The paper presents theoretical foundations for the logical sequence "catalase activity--intracellular substrate concentration--estimate of substrate consumed by bacteria" for experimental verification and as a consequence of development of the integral method of monitoring the bacterial population on the basis of determining bacterial catalase activity. Grant numbers: 04-96017, N25.     

Middle atmosphere summer duration as an indicator of long-term circulation changes

Summer duration (SD) is defined here as the time interval between spring and autumn turn around of zonal winds in the stratosphere. SD long-term trends are obtained from analysis of middle stratosphere NCEP and ECMWF data. They are found to be dependent on latitude and altitude. Wind data are available since 1948. The corresponding analysis suggests a breakpoint in the trend at around 1980: SD increases before 1980, and decreases afterwards. Corresponding changes of stratospheric wave activity are analyzed and found to be a major contribution to the SD trends. Long-term computer runs of the Whole Atmosphere Community Climate Model (WACCM 1b) are consistent with these results. Vegetation data on the ground indicate similar trends with a break.     

The pleurodele, an animal model for space biology studies.

Pleurodeles waltl, an Urodele amphibian is proposed as a model for space biology studies. Our laboratory is developing three types of experiments in space using this animal: 1) in vivo fertilization and development ("FERTILE" project); 2) influence of microgravity and space radiation on the organization and preservation of specialized structures in the neurons and muscle cells (in vitro; "CELIMENE" PROJECT); 3) influence of microgravity on tissue regeneration (muscle, bone, epidermis and spinal cord).     

The hydroxyl radical as an indicator of SEP fluxes in the high-latitude terrestrial atmosphere

The low background values at nighttime of the mesospheric hydroxyl (OH) radical make it easier to single out the atmospheric response to the external solar forcing in Polar Regions. Because of the short lifetime of HO_x, it is possible to follow the trails of Solar Energetic Particle (SEP) events in the terrestrial atmosphere, as shown by Storini and Damiani (2008). The sensitivity of this indicator makes discernible not only extreme particle events with a flux peak of several thousand pfu [1 pfu = 1 particle/(cm² s sr)] at energies >10 MeV, but also those with lower flux up to about 300 pfu. Using data from the Microwave Limb Sounder (MLS) on board the EOS AURA satellite, we examined the correlation of OH abundance vs. solar proton flux for almost all the identified SEP events spanning from November 2004 to December 2006 (later on no more SEP events occurred during Solar Cycle no. 23). The channels at energies greater than 5 MeV and 10 MeV showed the best correlation values (r ∼ 0.90–0.95) at altitudes around 65–75 km whereas, as expected, the most energetic channels were most highly correlated at lower altitudes. Therefore, it is reasonably possible to estimate the solar proton flux from values of mesospheric OH (and viceversa) and it could be useful in studying periods with gaps in the records of solar particles.     

Experimental and mathematical modeling of the consumer's influence on productivity of algae in a model aquatic ecosystem.

A "producer-consumer" (Chlorella vulgaris-Paramecium caudatum) closed aquatic system has been investigated experimentally and theoretically. It has been found that there is a direct relationship between the growth of the paramecia population and their release of ammonia nitrogen, which is the best form of nitrogen for Chlorella growth. The theoretical study of a model of a "producer-consumer" aquatic biotic cycle with spatially separated compartments has confirmed the contribution of paramecia to nitrogen cycling. It has been shown that an increase in the concentration of nitrogen released as metabolites of paramecia is accompanied by an increase in the productivity of microalgae.     

Use of an otolith-deficient mutant in studies of fish behavior in microgravity.

The mutant strain (ha) of medaka (Oryzias latipes) lack utricular otoliths as fry, and some never form otoliths for life. The cross (F1 generation) between the strain having good eyesight and another strain having ordinary eyesight augmented visual acuity of the F1 generation. Crossing the good eyesight strain and ha mutant produced fish having good eyesight and less sensitivity to gravity in the F2 population. Their tolerance to microgravity was tested by parabolic flight using an airplane. The fish exhibited less looping and no differences in degree of looping between light and dark conditions, suggesting that loss of eyesight (in darkness) is not a direct cause for looping behavior in microgravity. The ha embryos could not form utricular otoliths. They did form saccular otoliths, but with a delay. Fry of the mutant fish lacking the utricular otoliths are highly dependent on light upon hatching and exhibit a perfect dorsal-light response (DLR). As they grow, they eventually shift from being light-dependent to being gravity-dependent. Continuous treatment of the fry with altered light direction suppressed this shift to gravity dependence. Being less dependent on gravity, these fish can serve as models in studying the differences expected for the vestibular system of fish reared in microgravity. When these fish were exposed to microgravity (parabolic flights) of an airplane, they spent far less time looping than fish reared in an ordinary light regimen.     

The CEEF, closed ecosystem as a laboratory for determining the dynamics of radioactive isotopes.

Nuclear power generation is now confronted with a very difficult situation all over the world because of the problems of radioactive waste disposal and of the accidents, which have occurred. Nuclear power generation now supplies nearly 30% of total electric power demand in Japan. Therefore it is very difficult to change quickly the construction plans of nuclear facilities already designed. A nuclear fuel reprocessing center is now under construction in Rokkasho-Mura in Aomori Prefecture. If this center starts its operation, small amounts of 14CO2 are expected to be released into the atmosphere and will enter the global cycle. The simulation experiment of 14C trace amounts which enter into ecosystems is now being planned using stable isotope 13C within CEEF (Closed Ecology Experiment Facilities).     

The geomagnetic solar flare effect identified by SIIG as an indicator of a solar flare observed by GOES satellites

This paper studies the efficiency of geomagnetic solar flare effects (gsfe) in X solar flare detection; so during the period 1999–2007 a comparison between solar flare (sf) observed by satellites of the Geostationary Operational Environmental Satellite (GOES) programme and gsfe published by the Service International des Indices Geomagnetiques (SIIG) is made.     

基于飞机三维模型的装配单元快速划分方法

以航空企业实际调研为基础,在传统飞机装配单元划分经验知识的指导下结合当前飞机数字化技术开展基于飞机三维模型的装配单元快速划分技术研究,提出以三维工艺分离面作为数字化装配单元划分依据,建立其建模算法和流程,并在此基础上进行装配单元构件快速识别和建模研究,建立装配单元快速识别和建模算法,从而实现装配单元的快速划分.这些技术和方法已在CATIA V5系统上得到初步开发和验证.     

C.E.B.A.S. MINI MODULE: test results of an artificial (man-made) aquatic ecosystem.

The original Closed Equilibrated Biological Aquatic System (C.E.B.A.S.) is a long-term multi-generation research facility for experiments with aquatic animals and plants in a space station the development of which is surrounded by a large international scientific program. In addition, a miniaturized laboratory prototype, the C.E.B.A.S. MINI MODULE, with a total volume of about 10-12 liters for a Spacelab middeck locker was developed and a first version was tested successfully for two weeks with a population of fishes (Xiphophorus helleri) in the animal tank and a Ceratophyllum spec. in the illuminated higher plant growth chamber. The water recycling system consisted of a bacteria filter and a mechanical filter and the silastic tubing gas exchanger was separated by valves for the utilization in emergency cases only. Data were collected with the acquisition module of the original C.E.B.A.S. process control system. In addition, an optimized version was tested for 7 weeks with fishes and plants and thereafter with fish and with plants only for 2 and 1 weeks, resp.. The paper presents the relevant water parameters (e.g., pH, pressure, temperature, oxygen saturation, flow rate, ion concentrations) during the test period as well as morphological and physiological data of the enclosed animals and plants. On the basis of the given results the possible role of the C.E.B.A.S. system as a scientific tool in artificial ecosystem research and for the development of a combined animal-plant intensive aquaculture system and its utilization in bioregenerative life support is discussed.     

Phase separated membrane bioreactor: results from model system studies.

The operation and evaluation of a bioreactor designed for high intensity oxygen transfer in a microgravity environment is described. The reactor itself consists of a zero headspace liquid phase separated from the air supply by a long length of silicone rubber tubing through which the oxygen diffuses in and the carbon dioxide diffuses out. Mass transfer studies show that the oxygen is film diffusion controlled both externally and internally to the tubing and not by diffusion across the tube walls. Methods of upgrading the design to eliminate these resistances are proposed. Cell growth was obtained in the fermenter using Saccharomyces cerevisiae showing that this concept is capable of sustaining cell growth in the terrestrial [correction of terrestial] simulation.     

A "cryptic" microbial mat: a new model ecosystem for extant life on Mars.

If life were present on Mars to day, it would face potentially lethal environmental conditions such as a lack of water, frigid temperatures, ultraviolet radiation, and soil oxidants. In addition, the Viking missions did not detect near-surface organic carbon available for assimilation. Autotrophic organisms that lived under a protective layer of sand or gravel would be able to circumvent the ultraviolet radiation and lack of fixed carbon. Two terrestrial photosynthetic near-surface microbial communities have been identified, one in the inter- and supertidal of Laguna Ojo de Liebre (Baja California Sur, Mexico) and one in the acidic gravel near several small geysers in Yellowstone National Park (Wyoming, U.S.A.). Both communities have been studied with respect to their ability to fix carbon under different conditions, including elevated levels of inorganic carbon. Although these sand communities have not been exposed to the entire suite of Martian environmental conditions simultaneously, such communities can provide a useful model ecosystem for a potential extant Martian biota.     

Solar activity prediction studies and services in NAOC

Solar activity prediction services started in 1960’s in National Astronomical Observatories, Chinese Academy of Sciences (NAOC). As one of the members of the International Space Environment Service (ISES), Regional Warning Center of China (RWC-China) was set up in 1990’s. Solar Activity Prediction Center (SAPC), as one of the four sub-centers of RWC-China, is located in NAOC. Solar activity prediction studies and services in NAOC cover short-term, medium-term, and long-term forecast of solar activities. Nowadays, certain prediction models, such as solar X-ray flare model, solar proton event model, solar 10 cm radio flux model, have been established for the practical prediction services. Recently, more and more physical analyses are introduced in the studies of solar activity prediction, such as the magnetic properties of solar active regions and magnetic structure of solar atmosphere. Besides traditional statistics algorithms, Machine Learning and Artificial Intelligence techniques, such as Support Vector Machine (SVM) method, are employed in the establishment of forecast models. A Web-based integrated platform for solar activity data sharing and forecast distribution is under construction.     

The closed equilibrated biological aquatic system: a 12 months test of an artificial aquatic ecosystem.

The Closed Equilibrated Biological Aquatic System" (C.E.B.A.S.) is finally disposed for long-term multi-generation experiments with aquatic organisms in a space station. Therefore a minimum operation time of three months is required. It is verified in three versions of laboratory prototypes. The third one passed successfully a 12 months mid-term test in 1995/96 thus demonstrating its high biological stability. The third version of the C.E.B.A.S. consists of a 100 l animal tank, two plant cultivators with a volume of 15 l each with independent illuminations, a 3.0 l semibiological "mechanical" filter, a 3.0 l bacteria filter, a heating/cooling device and a dummy filter unit. The live-bearing teleost Xiphophorus helleri is the vertebrate and the pulmonate water snail Biomphalana glabrata the invertebrate experimental animal in the system. The rootless higher water plant Ceratophyllum demersum is the producer organism. Ammonia oxidizing bacteria and other microorganisms settle in the filters. A sample data acquisition is combined with temperature and plant illumination control. Besides of the space aspects the C.E.B.A.S. proved to be an extremely suitable tool to investigate the organism and subcomponent interactions in a well defined terrestrial aquatic closed ecosystem by providing physical, chemical and biological data which allow an approach to a comprehensive system analysis. Moreover the C.E.B.A.S. is the base for the development of innovative combined animal-plant aquaculture systems for human nutrition on earth which could be implemented into bioregenerative life support systems with a higher degree of complexity suitable for lunar or planetary bases.     

Crustaceans as a model for microgravity-induced muscle atrophy.

Atrophy of skeletal muscles is a serious problem in a microgravity environment. It is hypothesized that the unloading of postural muscles, which no longer must resist gravity force, causes an accelerated breakdown of contractile proteins, resulting in a reduction in muscle mass and strength. A crustacean model using the land crab, Gecarcinus lateralis, to assess the effects of spaceflight on protein metabolism is presented. The model is compared to a developmentally-regulated atrophy in which a premolt reduction in muscle mass allows the withdrawal of the large claws at molt. The biochemical mechanisms underlying protein breakdown involves both Ca(2+)-dependent and multicatalytic proteolytic enzymes. Crustacean claw muscle can be used to determine the interactions between shortening and unloading at the molecular level.     

Near-Earth radiation model deficiencies as seen on CRRES.

The Space Radiation (SPACERAD) experiments on the Combined Release and Radiation Effects Satellite (CRRES) gathered 14 months of radiation particle data in an 18 degrees inclination orbit between 350 km and 36000 km from July 1990 to October 1991. When compared to the NASA radiation belt models AP8 and AE8, the data show the proton model (AP8) does not take into account a second belt formed after major solar flare/shock injection events, and the electron model (AE8) is misleading, at best, in calculating dose in near-Earth orbits. The second proton belt, although softer in energy than the main proton belt, can produce upsets in proton sensitive chips and would produce significant dose in satellites orbiting in it. The MeV electrons observed on CRRES show a significant particle population above 5 MeV (not in the AE8 model) which must be included in any meaningful dose predictions for satellites operating between L-shells of 1.7 and 3.0 RE.