On the quality of mutations in mammalian cells induced by high LET radiations

The deleterious effects of accelerated heavy ions as component of the space radiation environment on living cells are of increasing importance for long duration human space flight activities. The most important aspect of such densely ionizing particle radiation is attributed to the type and quality of biological damage induced by them. This issue is addressed by investigating cell inactivation and mutation induction at the Hprt locus (coding for hypoxanthine-guanine-phosphoribosyl-transferase) of cultured V79 Chinese hamster cells exposed to densely ionizing radiation (accelerated heavy ions with different LETs from oxygen to gold, specific energies ranging from 1.9 to 69.7 MeV/u, corresponding LET values range from 62 to 13,223 keV/μm) and to sparsely ionizing radiation (200 kV X-rays). 30 spontaneous, 40 X-ray induced and 196 heavy ion induced 6-thioguanine resistant Hprt mutant colonies were characterized by Southern technique using the restriction enzymes EcoRI, PstI and BglII and a full length Hprt cDNA probe isolated from the plasmid pHPT12. Restriction patterns of the spontaneous Hprt mutants were indistinguishable from the wild type pattern, as these mutants probably contain only small deletions or even point mutations in the Hprt locus. In contrast, the overall spectrum of heavy ion induced mutations revealed a majority of partial or total deletions of the Hprt gene. With constant particle fluence (3 × 10⁶ particles/cm²) the quality of heavy ion induced mutations in the Hprt locus depends on physical parameters of the beam (atomic number, specific energy, LET). This finding suggests a relationship between the type of DNA damage and track structure. The fraction of mutants with severe deletions in the Hprt locus after exposure to oxygen ions increases from 65% at 60 keV/μm up to a maximum (100%) at 300 keV/μm and declines with higher LET values to 75% at 750 keV/μm. With heavier ions (Ca- and Au-ions) and even higher LET-values this mutant fraction decreases to 58% at 13,200 keV/μm. Heavy ion induced DNA break points in the Hprt locus are not randomly distributed.     

On some large solar energetic particle events: Direct impulsive component and broad associated coronal mass ejections

Using the proton intensity and X-ray flux data from the GOES, combined with the observations of the associated solar eruptions by the Large Angle and Spectrometric Coronagraph Experiment (LASCO) on board the Solar and Heliospheric Observatory (SOHO), 14 large SEP events occurring in the period 2000 January–2002 April have been studied. It is found that: (1) events with the SEPs increasing shortly after the maximum of their parent flares (<1 h; hereafter prompt events) have rapid and great (up to four orders of magnitude) SEP increments in high-energy channels (> ∼100 MeV); however, for events whose onset of the SEP injection lags the flare maximum for a long time (>3 h; hereafter delayed events), the high-energy SEPs show no obvious enhancements (within one order of magnitude); (2) peak intensity of the prompt events is distinctly larger than that of the delayed events; (3) CMEs associated with the poorly magnetically connected events (source region <W30°) in our survey are all halo CMEs. From these observational differences, we propose a special scenario of the production of the largest SEP events: both CMEs and flares are induced in the same coronal process; high-energy particles accelerated in the reconnection region can escape easily from the open field lines and/or be transported by fast CMEs into interplanetary space, indicating a direct impulsive component in large gradual SEP events. Meanwhile, the broad width of the associated CMEs implies that the CME width is more important in SEP events production than previously considered.     

Modelling of protons spectra encountered in space using medical accelerator and its microdosimetric characterization

Radiation environments in space are mainly composed of protons coming from the Galactic Cosmic Rays (GCRs) pervading the universe, the Solar Particle Events (SPEs) resulting from solar flares and coronal mass ejections, and the two Van Allen Belts surrounding the Earth due to the presence of the geomagnetic field trapping charged particles. Their wide spectra of energies up to hundreds of GeV imply diverse radiobiological effects to astronauts and radiation damage to electronics in the spacecraft. Even if lower in abundance, heavy ions such as He, C, O, Si, Fe are present in space and constitute an even bigger hazard due to their high penetrability and high linear energy transfer (LET). Most irradiation facilities available for research and testing worldwide provide usually only monoenergetic beams of high-energy protons or other heavier particles limiting studies of radiobiological effects and effects on electronics to a set of discrete energies.This paper introduces a procedure where a proton fluence spectra of interest for space radiation protection, previously generated by Monte Carlo simulations was delivered using a clinical proton therapy accelerator. Particularly, it reports the first results of modelling a proton radiation field in space in the energy range from 70 to 230 MeV during a single experimental session by programming a treatment planning system (TPS) to deliver required proton irradiation energies. Moreover, the angular distribution of the proton irradiation field has been varied to reproduce the isotropic exposure experienced by humans in space. The obtained proton radiation field was characterized using a 3D sensitive volume SOI microdosimeter developed by the Centre for Medical Radiation Physics (CMRP), University of Wollongong, Australia.     

Hypervelocity impacts on HST solar arrays and the debris and meteoroids population

Accurate debris and meteoroid flux models are crucial for the design of manned and unmanned space missions. For the most abundant particle sizes smaller than a few millimetres, knowledge of the populations can only be gained from in situ detectors or the analysis of retrieved space hardware. The measurement of impact flux from exposed surfaces improves with increased surface area and exposure time.A post-flight impact investigation was initiated by the European Space Agency to record and analyse the impact fluxes and any potential resulting damage on the two flexible solar arrays of the Hubble Space Telescope. The arrays were deployed during the first Hubble Space Telescope servicing mission in December 1993 and retrieved in March 2002. They have a total exposed surface area of roughly 120 m², including 42 m² covered with solar cells. This new Hubble post-flight impact study follows a similar activity undertaken after the retrieval of one of the first solar arrays, in 1993. The earlier study provided the first opportunity for a numerical survey of damage to exposed surfaces from more than 600 km altitude, and of impacts from particles larger than 1 mm. The results have proven very valuable in validation of important flux model regimes. The second set of Hubble solar arrays has again provided an unrivalled opportunity to measure the meteoroid and debris environment, now sampled during a long interval in low Earth orbit, and to identify changes in the space debris environment since the previous survey. The retrieved solar array wings exhibit thousands of craters, many of which are visible to the naked eye. A few hundred impacts have completely penetrated the 0.7 mm thick array. The largest impact features are about 7–8 mm in diameter. The cover glass of the solar cells is particularly well suited to the recognition of small impact features by optical and electron microscopy. In this paper, we present the first results of the impact survey. Data upon the abundance of craters of specific measured size ranges are plotted as cumulative flux curves, and compared to the results of model predictions. The most significant change to the particle flux since 1993 is a decrease in the small debris population.     

Analysis of electromagnetic and electrostatic effects of particle impacts on spacecraft

Particle impacts on spacecraft can cause considerable damage, even leading to complete failure. A theory for the resulting vehicle potential changes and the electromagnetic radiation from impact-induced plasma has been published by Close et al. (2010). Here we compare this theory to impacts registered by the Radio and Plasma Wave Science instrumentation on the Cassini spacecraft. We study both low-velocity (16 km/s) large particles (2.6 μm radius) detected in Saturn’s rings and high-velocity (450 km/s) small particles (1 nm radius) in the solar wind. The agreement with the theory is quite good. We also apply these results to earth orbit and conclude that both Electrostatic Discharge and Electromagnetic Pulse radiation are likely and could lead to spacecraft failure.     

Prospects for observing dark-matter remnants with GLAST

The satellite-based experiment, GLAST (Gamma-ray Large Area Space Telescope), is under construction and is planned to measure the cosmic γ-ray flux in the energy range 20 MeV to >300 GeV, with supporting measurements for γ-ray bursts from 10 keV to 25 MeV. With its launch in 2007, GLAST will open a new and important window on a wide variety of high-energy phenomena, including exotic relics from the Big Bang. Among these may be the decay/annihilation products of the hypothesized super symmetric image of the known particles. Single-photon energy thresholds for channels leading to such final states have been excluded in a model-dependent manner by accelerator searches to energies greater than 50 GeV. The ability of GLAST to set limits on this important component of cosmological evolution is presented along with an update on the present status of this mission.     

High energy emission from pulsars: Outer gap scenario

I will give a brief review of the recent development in the emission models of isolated, rapidly rotating neutron stars, focusing on the γ-ray radiation mechanism in their outer magnetospheres. By examining the Poisson equation for the electrostatic potential, I show that an active particle accelerator must extend from the vicinity of the neutron star surface to the vicinity of light cylinder. Furthermore, combining the Poisson equation with the Boltzmann equations for electrons/positrons and γ-rays, and assuming that the gap trans-field thickness is large compared to the longitudinal width, I demonstrate that the energy distribution of ultra-relativistic particles cannot be described by a power-law but by a quasi-monoenergetic distribution at the terminal Lorentz factor. The particles are accelerated in the gap and escape from it with large Lorentz factors. Is is shown that such energetic particles migrating outside of the gap contribute significantly to the γ-ray luminosity and reproduce the observed soft γ-ray spectrum between 100 MeV and 3 GeV for the Vela pulsar.     

Modeling of effect of polarization on UV sky radiance during twilight

The spatial distribution of the vector of the Stokes parameters characterizing the radiance intensity and the radiance polarization describes the radiation field in the atmosphere. A simplified treatment of light as the scalar has only restricted application. A few studies compared previously results of the vector and scalar radiative transfer models and showed that scalar models are in error by up to 10% for many cases. Though several observational conditions were exploited, an effect of polarization on modeling of UV radiance has not been investigated yet for twilight. The paper presents a preliminary study of modeled UV radiance during twilight taking into account polarization. The intensity and the degree of linear polarization of the scattered UV radiance for two cases of the ground-based observations are discussed. In the first case, radiation incoming from the zenith for the solar zenith angles (SZA) from 90° to 98° is under investigation. Radiation in the solar principal plane for the beginning of twilight (SZA = 90.1°) was calculated in the second case. The study showed that the UV radiation field in the twilight atmosphere can be handled correctly only using the vector theory. The errors of scalar radiative transfer strongly depend on wavelength, line of an observation and solar position. The revealed distortion of the zenith radiance caused by using of the scalar approximation reaches maximum of 15% at 340 nm for the solar zenith angle (SZA) equal to 98°. The shorter wavelengths have the smaller errors, about 5% at 305 nm for SZA = 98°, due to the larger part of the single scattered radiance. The error of the scalar modeling may be as large as −17% for radiance incoming from the horizon for SZA = 90.1°. Scalar radiative transfer models underestimate the integral intensity in the principal plane up to 3–4% ± 0.5% at SZA = 90.1° for wavelengths from 320 to 340 nm. This should be taken into account in problems of radiative budget estimation and remote sensing of the atmosphere exploiting the twilight period.     

What gamma-ray deexcitation lines reveal about solar-flares

Gamma-ray emission from solar flares reveals information about the nature of the accelerated particles and about the physical conditions of the medium through which the accelerated particles are transported. In this paper, we present the gamma-ray line-production and loop transport models used in our calculations of high-energy emission. We discuss the calculated interaction time history, the depth distribution, the interacting-particle angular distribution, and fluence ratios of the narrow gamma-ray lines. We show the relationship between the γ-ray observables and the parameters of the transport and line-production models. For illustration, we use calculations of 4.44 MeV ¹²C nuclear deexcitation line-production. Applications of the calculations to flare observations by both SMM and RHESSI are also presented.     

Giant radio pulses from the Crab pulsar

Individual giant radio pulses (GRPs) from the Crab pulsar last only a few microseconds. However, during that time they rank among the brightest objects in the radio sky reaching peak flux densities of up to 1500 Jy even at high radio frequencies. Our observations show that GRPs can be found in all phases of ordinary radio emission including the two high frequency components (HFCs) visible only between 5 and 9 GHz [Moffett, D.A., Hankins, T.H. Multifrequency radio observations of the Crab pulsar. Astrophys. J. 468, 779–783, 1996]. This leads us to believe that there is no difference in the emission mechanism of the main pulse (MP), inter pulse (IP) and HFCs. High resolution dynamic spectra from our recent observations of giant pulses with the Effelsberg telescope at a center frequency of 8.35 GHz show distinct spectral maxima within our observational bandwidth of 500 MHz for individual pulses. Their narrow band components appear to be brighter at higher frequencies (8.6 GHz) than at lower ones (8.1 GHz). Moreover, there is an evidence for spectral evolution within and between those structures. High frequency features occur earlier than low frequency ones. Strong plasma turbulence might be a feasible mechanism for the creation of the high energy densities of ∼6.7 × 10⁴ erg cm⁻³ and brightness temperatures of ∼10³¹ K.     

Nonlinear processes in cosmic-ray precursor of strong supernova shock: Maximum energy and average energy spectrum of accelerated particles

The instability in the cosmic-ray precursor of a supernova shock is studied. The level of turbulence in this region determines the maximum energy of accelerated particles. The consideration is not limited by the case of weak turbulence. It is assumed that the Kolmogorov type nonlinear wave interactions together with the ion-neutral collisions restrict the amplitude of random magnetic field. As a result, the maximum energy of accelerated particles strongly depends on the age of a SNR. The average spectrum of cosmic rays injected in the interstellar medium in the course of adiabatic SNR evolution takes the approximate form E⁻² at energies larger than 10–30 GeV/nucleon with the maximum energy that is close to the position of the knee in cosmic-ray spectrum at 4 × 10¹⁵ eV. At an earlier stage of SNR evolution – the ejecta-dominated stage, the particles are accelerated to higher energies and have a rather steep power-law distribution. These results suggest that the knee may mark the transition from the ejecta-dominated to the adiabatic evolution of SNR shocks which accelerate cosmic rays.     

An analysis of solar radio burst events on December 1, 2004

We present the analysis of the radio observations of December 1, 2004 from 07:00 UT to 07:40 UT in the 1.100–1.340 GHz band by Solar Broadband Radio Dynamic Spectrometer (SBRS) in Huairou Station. There are three groups of radio fine structures during the impulsive phase of this flare denoted by N1, Z2, and Z3. N1 has several emission lines with mixed fast and slow frequency drift rate which may reflect the conditions of flare loop and fast flows out from reconnection site; Z2 and Z3 are zebra patterns. The radio observations combined with hard X-ray and other observations show that the fine structures are connected with energetic particles. The information about magnetic field and energetic particle during the burst are also estimated based on our model.     

Mice heterozygous for the ATM gene are more sensitive to both X-ray and heavy ion exposure than are wildtypes

Previous studies have shown that the eyes of ATM heterozygous mice exposed to low-LET radiation (X-rays) are significantly more susceptible to the development of cataracts than are those of wildtype mice. The findings, as well as others, run counter to the assumption underpinning current radiation safety guidelines, that individuals are all equally sensitive to the biological effects of radiation. A question, highly relevant to human space activities is whether or not, in similar fashion there may exist a genetic predisposition to high-LET radiation damage.Mice haplodeficient for the ATM gene and wildtypes were exposed to 325 mGy of 1 GeV/amu ⁵⁶Fe ions at the AGS facility of Brookhaven National Laboratory. The fluence was equivalent to 1 ion per lens epithelial cell nuclear area. Controls consisted of irradiated wildtype as well as unirradiated wildtype and heterozygous mice. Prevalence analyses for stage 0.5–3.0 cataracts indicated that not only cataract onset but also progression were accelerated in the mice haplo-deficient for the ATM gene.The data show that heterozygosity for the ATM gene predisposes the eye to the cataractogenic influence of heavy ions and suggest that ATM heterozygotes in the human population may also be radiosensitive. This may have to be considered in the selection of individuals who will be exposed to both HZE particles and low-LET radiation as they may be predisposed to increased late normal tissue damage.     

Experimental and numerical simulation of super-pressure balloon apex section: Mechanical behavior in realistic flight conditions

In order to predict the flight parameters and to improve the life time of long duration super-pressure balloon, a research program on modelization and experimental simulation of the balloon envelope mechanical behavior is carried out.The balloon is a 10 m diameter type made with multilayer polymeric films and tapes. A facility was developed to measure, via a stereo-correlation system, the 3D displacement and the in plane solicitation strain of a 1.5 m diameter balloon envelope part in realistic flight conditions, i.e. pressure, temperature and loading at the sample boundaries. A time dependant non-linear Maxwell model of the polymeric material behavior was identified from uniaxial creep and relaxation tests and implemented in a Finite Elements code, simulating the sample tested in the facility. The Poisson ratio of the transparent and supple balloon film has been measured with an image correlation system.Experimental results are obtained both at room and in cold conditions (−60 °C) for various values of differential pressure.Vertical displacement and in plane 2D strain of apex part deduced from the numerical modeling are compared to experimental results.     

Energetic particle transport in anisotropic magnetic turbulence

We study energetic particle transport in a magnetic field configuration which models the solar wind magnetic turbulence plus the background field. A power-law Fourier amplitude is used for the fully 3D turbulence model, and in order to model anisotropic turbulence, the constant amplitude surfaces in k space are ellipsoids. The turbulence correlation lengths parallel (perpendicular) to the background magnetic field l_∥ (l_⊥) are varied in a wide range, and proton energies from 1 MeV to 10 GeV are assumed. Considering propagation on a distance corresponding to 1 AU, it is found that transport parallel and perpendicular to the background field heavily depends on the turbulence anisotropy, that is on the ratio l_∥/l_⊥. The spatial distribution of energetic particle follows the shape of magnetic flux tube up to about 10 MeV, while for larger energies the structure of the magnetic flux tube is progressively washed out. The scatterplots of particle distribution show intermittent, non Gaussian structures for l_∥ ≪ l_⊥ (quasi slab turbulence), while a more diffusive, Gaussian structure is obtained for l_∥ ≫ l_⊥ (quasi 2D turbulence). The long time behavior of transport shows that anomalous (subdiffusive perpendicular and superdiffusive parallel) transport regimes are obtained for l_∥ ≪ l_⊥, while Gaussian diffusive transport is obtained for both l_∥ ≫ l_⊥ and the isotropic turbulence case.     

Detection of meteors and sub-relativistic dust grains by the fluorescence detectors of ultra high energy cosmic rays

Fluorescence detectors of ultra high energy cosmic rays (UHECR) allow to record not only the extensive air showers, initiated by the UHECR particles, but also to detect light, produced by meteors and by the fast dust grains. It is shown that the fluorescence detector operated at the mountain site can register signals from meteors with kinetic energy threshold of about 25 J (meteor mass ∼ 5 × 10⁻⁶ g, velocity ∼ 3 × 10⁶ cm/s). The same detector might be used for recording of the dust grains of smaller mass (of about 10⁻¹⁰ g) but with velocity 10⁹ cm/s, close to the light velocity (sub-relativistic dust grains). The light signal from a sub-relativistic dust grain is expected in much shorter time scale (∼0.001 s), in comparison with the meteor signal (∼0.1–1 s), and much longer than duration of the UHECR signals (tens of μs). The fluorescence detector capable to register various phenomena: from meteors to UHECR – should have a variable pixel and selecting system integration time. A study of the new phenomenon of sub-relativistic grains will help to understand the mechanism of particle and dust grain acceleration in astrophysical objects (in SN explosions, for example).     

RIO: the R-process isotope observer

Our galaxy is filled with a “gas” of relativistic nuclei and electrons – the galactic cosmic rays (GCRs). The source of GCR nuclei is unknown, but there is a general consensus among high-energy astrophysicists that GCRs are accelerated by supernova (SN) shocks in the interstellar medium. The evidence in support of this picture is strong but indirect. However, the fact that the cosmic-ray spectrum extends continuously, without steps or peaks, to more than five orders of magnitude in energy beyond the limit of SN shock acceleration appears to be incompatible with this picture, at least without extreme fine-tuning of models. A “smoking gun” is needed to definitively establish that SN shocks are indeed the accelerator of GCRs. If GCRs are accelerated in SN shocks, they will be enhanced in freshly-synthesized r-process material. We are currently studying the R-process Isotope Observer (RIO) as a Mission of Opportunity for the International Space Station. RIO will make the first measurements of the isotopic abundances of the “ultraheavy” GCRs (those in the range 32 ⩽ Z ⩽ 42) and will determine the fractional contribution of freshly-synthesized r-process material in GCRs through the measurement of several key isotopic ratios.     

Study of >100 GeV electrons with BETS detector using a long duration balloon flight in Antarctica

We have observed cosmic-ray electrons from 10 to 1000 GeV by a long duration balloon flight using Polar Patrol Balloon (PPB) in Antarctica. The observation was carried out for 13 days at an altitude of 35 km in January 2004. The detector is an imaging calorimeter composed of scintillating-fiber belts and plastic scintillators inserted between lead plates. The geometrical factor of detector is about 600 cm²sr and the total thickness of lead absorber is 9 radiation lengths. The performance of the detector has been confirmed by the CERN-SPS beam test and also investigated by Monte-Carlo simulations. New telemetry system using a commercial satellite of iridium, power supply by solar batteries, and automatic level control using CPU have successfully been developed and operated during the flight. We have collected 5.7 × 10³ events over 100 GeV including nearly 100 candidates of primary electrons.     

Progress in laboratory performance verification of the R-process Isotope Observer

The source of galactic cosmic rays (GCR) remains unknown, despite many decades of research. It is generally accepted among high-energy astrophysicists that GCR are accelerated by supernova (SN) shocks in the interstellar medium, but the evidence for this remains indirect. However, the fact that the cosmic-ray spectrum continues far past the limit of SN shock acceleration is a serious problem for this model. Most SNae occur in superbubbles – regions of the interstellar medium containing massive stars and SN remnants. Thus, SNae should be accelerating the ejecta of other SNae. The local medium of the superbubble will be enhanced in freshly synthesized R-process material. We are currently developing the R-process Isotope Observer (RIO), which could make the first measurements of the isotopic abundances of the “ultraheavy” GCR (those in the range 32 ⩽ Z ⩽ 42) in order to determine the fractional contribution of freshly synthesized R-process material in GCR through the measurement of several key isotopic ratios. We have recently performed a series of tests of the RIO detector with 400 A MeV ⁸⁴Kr at the HIMAC accelerator in Japan. The results of these tests indicate that a mass resolution of ∼0.3 u can be achieved with RIO. We also discuss the prospects for accommodation of the RIO mission.     

Hard X-ray footpoint motions in solar flares: Comparing magnetic reconnection models with observations

Hard X-ray observations from the Reuven Ramaty High Energy Solar Spectroscopic Imager (RHESSI) of the October 29, 2003 GOES X10 two-ribbon flare are used together with magnetic field observations from the Michelson Doppler Imager (MDI) onboard SoHO to compare footpoint motions with predictions from magnetic reconnection models. The temporal variations of the velocity v of the hard X-ray footpoint motions and the photospheric magnetic field strength B in footpoints are investigated. The underlying photospheric magnetic field strength is generally higher (B ∼ 700–1200 G) in the slower moving (v ∼ 20–50 km s⁻¹) western footpoint than in the faster (v ∼ 20–100 km s⁻¹) moving eastern source (∼100–600 G). Furthermore, a rough temporal correlation between the HXR flux and the product vB² is observed.