Molecular aspects of adaptation to extreme cold environments

Here are reviewed and summarized the strategies adopted by living organisms to survive low temperatures, from a molecular and membrane point of view. The presentation is aimed at a wide variety of readers.

Two prime examples of connections between biological cold adaptation and the molecular level are (1) antifreeze proteins in fish from cold sea water, (the DNA sequence of the protein gene is now known) (2) the fluidity characteristics of cell membranes in a wide variety of organisms. In model membranes of phospholipids, stabler “s-phases” have recently been found to form at low temperatures. Antarctic endolithic organisms, living just under the surface of rocks, are exposed to long periods of low temperatures, and may develop such phases in their membranes. In the saturated phosphatidyl cholines, only lipids with a restricted range of acyl chain lengths show simultaneously s-phases and a main transition : This restricted range is about the restricted range found in natural membranes. The s-phases also form in the presence of natural cryoprotectants, and may be connected with botanical vernalization.     

Meteorites in Antarctica — Statistics on falls, concentration, recovery and alteration on ice-sheet

The Antarctic meteorites are distributed on the blue-ice area surfaces in the ablation zone of the Antarctic ice-sheet, to where meteorites have been transported by the ice-flow within the ice-sheet from the wider accumulation zone. Among the Antarctic meteorite collection H- and L-chondrites are most abundant; this is also true in the non-Antarctic meteorite collection. Meteorite showers also are involved in the collection. Several new types of stony meteorites have been discovered from the Antarctic meteorite collection. The mass and shape of Antarctic meteorites are in agreement with those of resultant fragments of high-speed impact basaltic rocks. In Antarctica, small fragments of meteorite smaller than 1 kg in weight can easily be found and collected. The solidification and the gas retention ages of Antarctic meteorites also are concentrated around 4.5×10⁹ years, but some of them are considerably younger. Their cosmic-ray exposure ages are extended up to 9×10⁶ years and their terrestrial ages are 9×10⁴-7×10⁵ years.     

Significance of rhizosphere microorganisms in reclaiming water in a CELSS.

Plant-microbe interactions, such as those of the rhizosphere, may be ideally suited for recycling water in a Controlled Ecological Life Support System (CELSS). The primary contaminant of waste hygiene water will be surfactants or soaps. We identified changes in the microbial ecology in the rhizosphere of hydroponical1y grown lettuce during exposure to surfactant. Six week old lettuce plants were transferred into a chamber with a recirculating hydroponic system. Microbial density and population composition were determined for the nutrient solution prior to introduction of plants and then again with plants prior to surfactant addition. The surfactant Igepon was added to the recirculating nutrient solution to a final concentration of 1.0 g L-1. Bacteria density and species diversity of the solution were monitored over a 72-h period following introduction of Igepon. Nine distinct bacterial types were identified in the rhisosphere; three species accounted for 87% of the normal rhizosphere population. Microbial cell number increased in the presence of Igepon, however species diversity declined. At the point when Igepon was degraded from solution, diversity was reduced to only two species. Igepon was found to be degraded directly by only one species found in the rhizosphere. Since surfactants are degraded from the waste hygiene water within 24 h, the potential for using rhizosphere bacteria as a waste processor in a CELSS is promising.     

Study of long path VLF signal propagation characteristics as observed from Indian Antarctic station,Maitri

To examine the quality and propagation characteristics of the Very Low Frequency (VLF) radio waves in a very long propagation path, Indian Centre for Space Physics, Kolkata, participated in the 27th Indian scientific expedition to Antarctica during 2007–2008. One Stanford University made AWESOME VLF receiving system was installed at the Indian Antarctic station Maitri and about five weeks of data were recorded successfully from the Indian transmitter VTX and several other transmitting stations worldwide. The quality of the signal from the VTX transmitter was found to be very good, consistent and highly stable in day and night. The signal shows the evidences of the presence of the 24 h solar radiation in the Antarctic region during local summer. Here we report the both narrow band and broadband VLF observations from this site. The diurnal variations of VTX signal (18.2 kHz) are presented systematically for Antarctica path and also compared the same with the variations for a short propagation path (VTX-Kolkata). We compute the spatial distribution of the VTX signal along the VTX-Antarctica path using the most well-known LWPC model for an all-day and all-night propagation conditions. The calculated signal amplitudes corresponding to those conditions relatively corroborate the observations. We also present the attenuation rate of the dominant waveguide modes corresponding to those propagation conditions where the effects of the Antarctic polar ice on the attenuation of different propagating waveguide modes are visible.     

Satellite observations of sea ice

An overview is presented of Antarctic and Arctic sea ice studies using data from the Nimbus-5 ESMR and the Nimbus-7 SMMR passive microwave radiometers. Four years (1973–1976) of ESMR data for the Antarctic Ocean define the characteristics of the seasonal cycle including regional contrasts and interannual variations. Major advances include the discovery of the Weddell polynya and the presence of substantial areas of open water in the Antarctic winter pack ice. Regional differences in sea ice extent on time-scales of about a month are shown to be associated with variations in surface-wind fields. In the Arctic, the computation of sea ice concentration is complicated by the presence of multiyear ice, but the amount of multiyear ice becomes an important measurable quantity with dual-polarized, multifrequency passive microwave sensors. Analysis of SMMR data demonstrates its advantage for studying the spatial and temporal variability of the Arctic ice cover. Large observed interannual variations in the distribution of the multiyear pack ice and the presence of significant divergent areas in the central Arctic during winter contrast markedly with the classical view of the Arctic pack ice.     

Italian polar data center for capacity building associated with the IHY

The International Heliophysical Year offers a good opportunity to develop and coordinate studies on the Sun–Earth system by using a large variety of simultaneous data obtained by satellite/spacecraft and ground based instruments. Among these data we recall the ones coming from solar and interplanetary medium observations, auroral, neutron monitor, geomagnetic field, ionospheric, meteorological, and other atmospheric observatories. In this context, an Information System for the Italian Research in Antarctica has started in 2003, aiming to collect information on the scientific research projects funded by the National Antarctic Research Program of Italy since its establishment (1985). It belongs to Joint Committee on Antarctic Data Management of Scientific Committee on Antarctic Research as Italian Antarctic Data Center. This project, as the Italian Polar Database, gathers also information on research activities conducted in North Pole regions. This Information System can be a relevant resource for capacity building associated with the International Heliophysical Year, especially for people involved in interdisciplinary researches. We describe the present status of the Italian Polar Data Center and its potential use.     

Mini-belt as a fine spatial structure of the outer radiation belt in quiet and disturbed conditions

Electron flux data from LANL geostationary spacecrafts were statistically treated and ordered in a special magnetic coordinate system (effective L-coordinate and MLT). The data treating procedure allowed to obtain the dynamics of quasi-trapped electrons of different energies on effective L-shells ranging from 6.6 to 7.0. It was found that in quiet conditions a stable fine spatial structure of quasi-trapped electrons exists with maximum of fluxes near L = 6.78 and MLT=12. This structure may be looked at as an asymmetrical “mini-belt”. The position of the maximum depends on electron energy and changes with magnetic activity. The dynamics of this mini-belt for both quiet and disturbed periods is illustrated and discussed. During isolated magnetic storms the mini-belt maximum shifts in a regular manner outward and inward; a diffusion wave of quasi-trapped particles propagates from outside of the geostationary orbit and serves as a source of new particles for the mini-belt. The azimuthal geometry of this diffusion wave extracted from experimental data is illustrated. The possible role of the “mini-belt” is discussed in relation with well-known “anomalous” dynamics of the inner radiation belt.     

Energetic neutron and gamma-ray spectra under the earth radiation belts according to “SALUTE-7”-“KOSMOS-1686” orbital complex and “CORONAS-i” satellite data

The spectra of neutrons >10 MeV and gamma-rays 1.5–100 MeV under the Earth Radiation Belts, restored from the data, obtained onboard orbital complex “SALUTE-7”-“KOSMOS-1686”, are presented. The spectra shapes are similar to those for albedo neutrons and gamma-rays, but absolute values of their fluxes (0.2 cm⁻² s⁻¹ for neutrons, 0.8 cm⁻² s⁻¹ for gamma-rays at the equator and 1.2 cm⁻² s⁻¹, 1.9 cm⁻² s⁻¹, accordingly, at L=1.9) are several times as large. It is possibly explained by the fact that most of the detected particles were produced by the cosmic ray interactions with the orbital complex matter. Neutron and gamma-ray fluxes obtained from “CORONAS-I” data are near those for albedo particles.     

Mercury: can any ice exist at subpolar regions?

The heat transfer in a regolith subsurface layer of thickness 20 m has been theoretically simulated for the areas near Mercury's north pole aiming at the clarification of the possible existence of subsurface ice formations of different form. The paper considers different models of the icy regolith structure and composition: pure uniform amorphous ice; a porous dispersive system with ice-filled pores and voids; permafrost. For comparison the heat transfer in dry iceless regolith has been considered as well. It has been shown that the line of maximum distribution of subsurface icy formations depends on the icy regolith model, but for any one in the “hot” regions it does not go below 70°. For the “cool” regions this line has been shown to go from 5° to 10° southward than that for the “hot” ones. The possible thickness of icy regolith near the pole has been estimated for different models assuming an interior heat flow of 15 mW m⁻². It has been shown that the maximum thickness of this layer takes place at the pole and is equal to 10 km for any model.     

Establishment of atmospheric weighted mean temperature model in the polar regions

Due to the special geographical location and extreme climate environment, the polar regions (Antarctic and Arctic) have an important impact on global climate change. Atmospheric weighted mean temperature (Tm) is a crucial parameter in the retrieval of precipitable water vapor (PWV) from the zenith wet delay (ZWD) of ground-based Global Navigation Satellite System (GNSS) signal propagation. In this paper, the correlation between weighted mean temperature and surface temperature (Ts) is studied firstly. It is shown that the correlation coefficients between Tm and Ts are 0.93 in the Antarctic and 0.94 in the Arctic. The linear regression Tm model and quadratic function Tm model of the Antarctic and the Arctic are established respectively using the radiosonde profiles of 12 stations in the Antarctic and 58 stations in the Arctic from 2008 to 2015. The accuracies of the linear regression Tm model, the quadratic function Tm model and GPT2w Tm model which is a state-of-the-art global Tm model are verified using the radiosonde profiles from 2016 to 2018 in the Antarctic and Arctic. Root Mean Square (RMS) errors of the linear regression Tm model, the quadratic function Tm model and GPT2w Tm model in the Antarctic are 3.07 K, 2.87 K and 4.32 K respectively, and those in the Arctic are 3.53 K, 3.38 K and 4.82 K, which indicates that the quadratic function Tm model has a higher accuracy compared to linear regression Tm model, and the accuracies of the two regional Tm models are better than that of GPT2w Tm model in the polar regions. In order to better evaluate the accuracy of Tm in the PWV retrieval, the PWV values of radiosondes are used for comparisons as the reference value. The RMS errors of PWV derived from the two Tm models are similar for 1.28 mm in the Antarctic and 1 mm in the Arctic respectively. In addition, the spatial and temporal variation characteristics of Tm are analyzed in the polar regions by spectral analysis of Tm data using fast Fourier transform. The results show that the Tm has obvious seasonality and annual periodicity in the polar regions, and the maximum difference between warm season and cold season is about 63 K. After comparing and analyzing the influences of latitude, longitude and elevation on the Tm in the polar regions, it is found that latitude and elevation have a greater influence on the Tm than the longitude. As the latitude and elevation increase, the Tm decreases, and vice versa in the polar regions.     

On the stereoselective aminoacylation of RNA

Aminoacylation of the “internal” 2′-hydroxyl groups of poly(A) with the imidazolide of racemic DL-leucine resulted in the formation of an enantiomeric excess of the D-ester, whereas aminoacylation with the imidazolide of racemic N-3,5-dinitrobenzoyl-DL-leucine gave an enantiomeric excess of the L-ester. Comparison of these results with those obtained earlier for alanine shows that the larger side chain tends to favor the formation of the D-ester. High resolution proton magnetic resonance spectroscopy was performed on the D- and L-alanyl “internal” 2′-esters of 3′,5′-ApA. The resonance of the side chain methyl group of the D-ester is upfield from that of the L-ester, and the separation of the peaks increases with a decrease in temperature.     

Behavioural and biochemical investigations of the influence of altered gravity on the CNS of aquatic vertebrates during ontogeny.

Quantitative data are presented on the influences of hyper-gravity (3 +/- 1g) and of simulated weightlessness (approximately 0g) during early ontogeny of cichlid fish (Oreochromis mossambicus) and clawed toad (Xenopus laevis, Daudin) demonstrating changes in the swimming behaviour and the brain energy and plasma membrane metabolism. After return to 1g conditions, hyper-g reared fish and toads express the well known "loop-swimming" behaviour. By means of a computer based video analyzing system different types of swimming movements and velocities were quantitatively determined. Analyses of the brain energy and plasma-membrane metabolism of hyper-g fish larvae demonstrated an increase in energy availability (glucose 6Pi dehydrogenase, G-6P-DH), a decrease of cellular energy transformation (creatine kinase activity, CK) but no changes in energy consumptive processes (e.g. ATPases) and cytochrome oxidase activity (Cyt.-Ox). In contrast hypo-g fish larvae showed a slight increase in brain CK activity. In addition, unlike 1g controls, hyper-g fish larvae showed pronounced variations in the composition (=polarity) of sialoglycosphingolipids (=gangliosides), typical constituents of the nerve cell membranes, and a slight increase in the activity of sialidase, the enzyme responsible for ganglioside degradation.     

Microflora in the basal strata at Antarctic ice core above the Vostok lake.

The microbiological investigations of the Antarctic ice core at the Vostok station become especially important in connection with the discovery of an subglacial lake in this region. This lake is considered by the world-wide scientific community to be an important object for searching for relict forms of life on the Earth and also as a model for solving a number of problems of exobiology--for instance for development of methods to penetrate into underice sea at Europe--Jupiter's satellite. For the first time the Antarctic ice core samples were taken from the horizons which correspond to the basal zone (3534-3541 m) and to the accreation ice zone (3555-3611 m) above the subglacial lake Vostok. As a result of the microbiological investigations it was shown that the total number of microbial cells have been in the same range of quantities as at the upper, younger horizons and varied from 1.3 x 10(2) up to 9.6 x 10(2) cl/ml. Some periodicity in the cell concentration and in their morphological diversity was revealed along the core. The maximal number and the greatest morphological variety were detected at horizons with the depth of 3534, 3555 and 3595 m. A drop in the cell concentration two or three times as much was found in ice layers under each of the above mentioned horizons. The discovered stratification is apparently connected with the periodicity of the lake water interactions with the basal ice layer and obviously depends on the complex natural events which took place in the geological history of our planet.     

Cold resistance and metabolic activity of lichens below 0°C

Laboratory measurements show that lichens are extremely tolerant of freezing stress and of low-temperature exposure. Metabolic activity recovered quickly after severe and extended cold treatment. Experimental results demonstrate also that CO₂ exchange is already active at around −20°C. The psychrophilic character of polar lichen species is demonstrated by optimum temperatures for net photosynthesis between 0 and 15°C. In situ measurements show that lichens begin photosynthesizing below 0°C if the dry thalli receive fresh snow. The lowest temperature measured in active lichens was −17°C at a continental Antarctic site. The fine structure and the hydration state of photobiont and mycobiont cells were studied by low-temperature scanning electron microscopy (LTSEM) of frozen hydrated specimens. Water potentials of the frozen system are in the range of or even higher than those allowing dry lichens to start photosynthesis by water vapor uptake at +10°C. The great success of lichens in polar and high alpine regions gives evidence of their physiological adaptation to low temperatures. In general lichens are able to persist through glacial periods, but extended snow cover and glaciation are limiting factors.     

Microbial life at extremely low nutrient levels

Many microorganisms (“oligotrophs”) grow in distilled water: spp., spp., spp., spp., spp., , spp., ( ) , spp., yeasts, and spp. Also, certain lower fungi can be found here. In the laboratory, these organisms thrive on contaminations of the air (CO, hydrocarbons, H₂, alcohols etc.). All are euryosmotic and often grow also in higher concentrations of salts and nutrients. Natural locations with extremely low nutrient levels (snow, rain water pools, springs, free ocean water, Antarctic rocks and soils) do not contain more than 1–5 mg/1 of organic carbon. Oligotrophs found here are especially adapted to constant famine: they frequently live attached to surfaces, form polymers and storage products even while starving, and often aggregate. Many of these oligotrophs alter their morphology (surface to volume ratio) with changing nutrient concentrations. Extreme oligotrophs also occur in generally nutrient-rich environments such as sewage aeration tanks or compost soil. Here they are thought to survive in nutrient-depauperate microhabitats.     

Theromonuclear flashes in accreting neutron star envelopes

The thermonuclear model for x-ray burster and “soft transient” is discussed. The mass accretion rate M, the chemical composition of accreted matter, the gravitational potential of the neutron star and thermal state of outer layer / or core/ are the main parameters which determine the burning regime. Importance of nuclear network used for the properties of subsequent flashes is pointed out.     

The pGAPS experiment: An engineering balloon flight of prototype GAPS

The General Anti-Particle Spectrometer (GAPS) project is being carried out to search for primary cosmic-ray antiparticles especially for antideuterons produced by cold dark matter. GAPS plans to realize the science observation by Antarctic long duration balloon flights in the late 2010s. In preparation for the Antarctic science flights, an engineering balloon flight using a prototype of the GAPS instrument, “pGAPS”, was successfully carried out in June 2012 in Japan to verify the basic performance of each GAPS subsystem. The outline of the pGAPS flight campaign is briefly reported.     

The X-ray properties of accreting black holes

Accreting black holes in binary systems exhibit two properties that distinguish them from the vast majority of binary X-ray sources: (1) rapid ≤ 1 s variability and (2) bi-modal spectral behaviour. These findings are based primarily on the observed properties of Cyg X-1 where an estimate of the X-ray source mass from radial velocity studies indicates a mass of 10 M. The recently suggested black hole candidacy of LMC X-3 is based on a similar mass estimate. The X-ray properties are similar to those of Cyg X-1 in its “high state”. The unique spectral properties of these systems are used to search for other similar systems, and new “possible” candidates are suggested, that include several transient sources. A possible spectral connection between these systems and their more massive counterparts in AGN is discussed.     

Effects of slow clinorotation on lipid contents and proton permeability of thylakoid membranes of pea chloroplasts.

Photochemical characteristics and lipid composition of thylakoid membranes from 12 day-old pea leaves that were exposed to slow clino-rotation were examined and compared with a vertical control. Proton permeability of thylakoid membranes was estimated from light-induced proton uptake (delta H+) and post-illumination proton efflux in chloroplast suspensions. The delta pH magnitude was calculated from the level of light-induced quenching of 9-aminoacridine fluorescence. Proton permeability of thylakoid membranes increased during exposure to clino-rotation. When subsequently transferred to darkness, proton efflux increased almost 2-fold in clinorotated leaves. The results were compared with data on pigment and polar lipid composition of photosynthetic membranes in clino-rotated and control plants. It was concluded that both the increase of proton permeability and the decrease of polar lipid content in chloroplasts were induced by clino-rotation.